PDBsum entry: 2c4m

Transferase

PDB id: 2c4m

Contents

Protein chains

789 a.a. *

Ligands

PLP ×4

PO4 ×9

FMT ×19

EDO ×3

Waters ×1154

* Residue conservation analysis

PDB id:

2c4m

Name:

Transferase

Title:

Starch phosphorylase: structural studies explain oxyanion- dependent kinetic stability and regulatory control.

Structure:

Glycogen phosphorylase. Chain: a, b, c, d. Synonym: starch phosphorylase. Engineered: yes. Mutation: yes. Other_details: plp (1634) cofactor is covalently linked to lys634 via schiff base (c4a-nz)

Source:

Corynebacterium callunae. Organism_taxid: 1721. Atcc: dsm 20145. Expressed in: escherichia coli. Expression_system_taxid: 562. Expression_system_cell_line: xl1 blue.

Resolution:

1.90Å R-factor: 0.216 R-free: 0.232

Authors:

A.Purvis,B.Nidetzky,K.Watson

Key ref:

A.Purvis et al. Starch phosphorylase: structural studies explain oxyanion-Dependent kinetic stability and regulatory control. To be published,

Date:

20-Oct-05 Release date: 27-Feb-07

Protein chains

Q8KQ56  (Q8KQ56_9CORY) -  Phosphorylase

Seq: 796 a.a.

Struc: 789 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.2.4.1.1 - Phosphorylase.
• Pathway: Glycogen
• Reaction: (1,4-alpha-D-glucosyl)(n) + phosphate = (1,4-alpha-D-glucosyl)(n-1) + alpha-D-glucose 1-phosphate

(1,4-alpha-D-glucosyl)(n) + phosphate (Bound ligand (Het Group name = PO4) corresponds exactly) = (1,4-alpha-D-glucosyl)(n-1) + alpha-D-glucose 1-phosphate (Bound ligand (Het Group name = PLP) matches with 63.00% similarity)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

 Gene Ontology (GO) functional annotation 

• Biological process: carbohydrate metabolic process (1 term)
• Biochemical function: transferase activity (4 terms)