PDBsum entry 1z1f

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Transferase PDB id
Protein chain
388 a.a. *
Waters ×87
* Residue conservation analysis
PDB id:
Name: Transferase
Title: Crystal structure of stilbene synthase from arachis hypogaea (resveratrol-bound form)
Structure: Stilbene synthase. Chain: a. Engineered: yes
Source: Arachis hypogaea. Peanut. Organism_taxid: 3818. Expressed in: escherichia coli. Expression_system_taxid: 562.
Biol. unit: Dimer (from PDB file)
2.90Å     R-factor:   0.192     R-free:   0.252
Authors: Y.Shomura,I.Torayama,D.Y.Suh,T.Xiang,A.Kita,U.Sankawa,K.Miki
Key ref:
Y.Shomura et al. (2005). Crystal structure of stilbene synthase from Arachis hypogaea. Proteins, 60, 803-806. PubMed id: 16028220 DOI: 10.1002/prot.20584
03-Mar-05     Release date:   30-Aug-05    
Go to PROCHECK summary

Protein chain
Pfam   ArchSchema ?
Q9SLV5  (Q9SLV5_ARAHY) -  Resveratrol synthase
389 a.a.
388 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     metabolic process   2 terms 
  Biochemical function     catalytic activity     4 terms  


DOI no: 10.1002/prot.20584 Proteins 60:803-806 (2005)
PubMed id: 16028220  
Crystal structure of stilbene synthase from Arachis hypogaea.
Y.Shomura, I.Torayama, D.Y.Suh, T.Xiang, A.Kita, U.Sankawa, K.Miki.
No abstract given.

  Selected figure(s)  
Figure 1.
Figure 1. (A) Ribbon diagram of the homodimeric structure of peanut STS-resveratrol with each monomer colored separately. Bound resveratrols are depicted in ball-and-stick models. Structurally divergent areas, a1, a2, and a3, are highlighted in green in the right monomer, and the N- and C-termini are labeled. Figures are produced with DINO[12] and POV-Ray.[13] (B) Enlarged view of the active site pockets in STS-apo and STS-resveratrol in the indicated colors. An F[o] - F[c] difference Fourier electron density map calculated with data from a resveratrol-derivative crystal is indicated in brown (contoured at 1.5 ), and the refined model of resveratrol is depicted in magenta. Catalytic-triad residues[4] (Cys164, His303, and Asn336) are indicated in red characters. (C) Sequence alignments around Met98 and Thr132 for peanut STS1 (GeneBank accession code: AB027606), pine STS1 (Swiss-Prot accession code: P48407), alfalfa CHS2 (Swiss-Prot accession code: P30074), and pine CHS1 (Swiss-Prot accession code: O65872). Unvaried residues are colored in red. The residues belonging to a1 is enclosed with a blue frame. (D) Comparison among the structures of peanut STS, pine STS (PDB code: 1U0U, chain A), and alfalfa CHS (1BL5, chain A) around the area a1. Each model is indicated in a different color, and water molecules found in the peanut STS structure are depicted with red balls. An F[o] - F[c] difference Fourier electron density map calculated with data from a peanut STS-apo crystal, and the model without waters and corresponding residues is indicated in orange (contoured at 1.8 ). The hydrogen-bond network found in the peanut STS structure is indicated with dotted lines of magenta and the bond lengths are shown in Angstrom units.
  The above figure is reprinted by permission from John Wiley & Sons, Inc.: Proteins (2005, 60, 803-806) copyright 2005.  

Literature references that cite this PDB file's key reference

  PubMed id Reference
20358127 I.Abe, and H.Morita (2010).
Structure and function of the chalcone synthase superfamily of plant type III polyketide synthases.
  Nat Prod Rep, 27, 809-838.  
19507202 M.Tosin, D.Spiteller, and J.B.Spencer (2009).
Malonyl carba(dethia)- and malonyl oxa(dethia)-coenzyme A as tools for trapping polyketide intermediates.
  Chembiochem, 10, 1714-1723.  
18476876 O.Yu, and J.M.Jez (2008).
Nature's assembly line: biosynthesis of simple phenylpropanoids and polyketides.
  Plant J, 54, 750-762.  
17109150 B.Liu, T.Raeth, T.Beuerle, and L.Beerhues (2007).
Biphenyl synthase, a novel type III polyketide synthase.
  Planta, 225, 1495-1503.  
17698806 J.R.Gledhill, M.G.Montgomery, A.G.Leslie, and J.E.Walker (2007).
Mechanism of inhibition of bovine F1-ATPase by resveratrol and related polyphenols.
  Proc Natl Acad Sci U S A, 104, 13632-13637.
PDB codes: 2jiz 2jj1 2jj2
16551366 K.T.Watts, P.C.Lee, and C.Schmidt-Dannert (2006).
Biosynthesis of plant-specific stilbene polyketides in metabolically engineered Escherichia coli.
  BMC Biotechnol, 6, 22.  
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