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PDBsum entry 1yis

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protein ligands links
Lyase PDB id
1yis
Jmol
Contents
Protein chain
421 a.a. *
Ligands
SO4
Waters ×228
* Residue conservation analysis
PDB id:
1yis
Name: Lyase
Title: Structural genomics of caenorhabditis elegans: adenylosuccin
Structure: Adenylosuccinate lyase. Chain: a. Synonym: fumarate lyase (53.6 kd) (1h824). Engineered: yes
Source: Caenorhabditis elegans. Organism_taxid: 6239. Expressed in: escherichia coli. Expression_system_taxid: 562.
Biol. unit: Tetramer (from PDB file)
Resolution:
2.40Å     R-factor:   0.174     R-free:   0.202
Authors: J.Symersky,N.Schormann,S.Lu,Y.Zhang,E.Karpova,S.Qiu,W.Huang, J.Zhou,M.Luo,A.Arabshahi,A.Mckinstry,C.-H.Luan,D.Luo,D.John J.Tsao,L.Delucas,Q.Shang,R.Gray,S.Li,T.Bray,Y.-J.Chen,South Collaboratory For Structural Genomics (Secsg)
Key ref: J.Symersky et al. Structural genomics of caenorhabditis elegans: adenylosuccinate lyase. To be published, .
Date:
12-Jan-05     Release date:   25-Jan-05    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
Q21774  (PUR8_CAEEL) -  Adenylosuccinate lyase
Seq:
Struc:
478 a.a.
421 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: E.C.4.3.2.2  - Adenylosuccinate lyase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]

      Pathway:
Purine Biosynthesis (late stages)
      Reaction:
1. N6-(1,2-dicarboxyethyl)AMP = fumarate + AMP
2. (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4- carboxamido)succinate = fumarate + 5-amino-1-(5-phospho-D- ribosyl)imidazole-4-carboxamide
N(6)-(1,2-dicarboxyethyl)AMP
= fumarate
+ AMP
(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4- carboxamido)succinate
= fumarate
+ 5-amino-1-(5-phospho-D- ribosyl)imidazole-4-carboxamide
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     'de novo' AMP biosynthetic process   3 terms 
  Biochemical function     catalytic activity     4 terms