PDBsum entry: 1x6n

Hydrolase

PDB id: 1x6n

Contents

Protein chain

539 a.a. *

Ligands

AO3

Waters ×185

* Residue conservation analysis

PDB id:

1x6n

Name:

Hydrolase

Title:

Crystal structure of s. Marcescens chitinase a mutant w167a in complex with allosamidin

Structure:

Chitinase a. Chain: a. Engineered: yes. Mutation: yes

Source:

Serratia marcescens. Organism_taxid: 615. Gene: chia. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

2.00Å R-factor: 0.214 R-free: 0.238

Authors:

N.N.Aronson Jr.,B.A.Halloran,M.F.Alexyev,X.E.Zhou,Y.Wang, E.J.Meehan,L.Chen

Key ref:

N.N.Aronson jr. et al. Muation of trp167 at the -3 subsite of the chitin-Binding cleft of s. Marcescens chitinase a causes enhanced transglycosylation. To be published,

Date:

11-Aug-04 Release date: 26-Jul-05

Protein chain

P07254  (CHIA_SERMA) -  Chitinase A

Seq: 563 a.a.

Struc: 539 a.a.*

* PDB and UniProt seqs differ at 8 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.2.1.14 - Chitinase.
• Reaction: Hydrolysis of the 1,4-beta-linkages of N-acetyl-D-glucosamine polymers of chitin.

 Gene Ontology (GO) functional annotation 

• Biological process: carbohydrate metabolic process (2 terms)
• Biochemical function: catalytic activity (4 terms)