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PDBsum entry 1wze

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protein ligands Protein-protein interface(s) links
Oxidoreductase PDB id
1wze
Jmol
Contents
Protein chains
327 a.a. *
Ligands
NAD ×2
Waters ×438
* Residue conservation analysis
PDB id:
1wze
Name: Oxidoreductase
Title: Structural basis for alteration of cofactor specificity of malate dehydrogenase from thermus flavus
Structure: Malate dehydrogenase. Chain: a, b. Engineered: yes. Mutation: yes. Other_details: malate dehydrogenase mutant ex7
Source: Thermus thermophilus. Organism_taxid: 274. Strain: at-62. Gene: mdh. Expressed in: escherichia coli. Expression_system_taxid: 562.
Biol. unit: Dimer (from PQS)
Resolution:
2.00Å     R-factor:   0.193     R-free:   0.228
Authors: T.Tomita,S.Fushinobu,T.Kuzuyama,M.Nishiyama
Key ref: T.Tomita et al. Structural basis for alteration of cofactor specificity of malate dehydrogenase from thermus flavus. To be published, .
Date:
04-Mar-05     Release date:   14-Mar-06    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
P10584  (MDH_THETH) -  Malate dehydrogenase
Seq:
Struc:
327 a.a.
327 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 7 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: E.C.1.1.1.37  - Malate dehydrogenase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]

      Pathway:
Citric acid cycle
      Reaction: (S)-malate + NAD+ = oxaloacetate + NADH
(S)-malate
+
NAD(+)
Bound ligand (Het Group name = NAD)
matches with 59.00% similarity
= oxaloacetate
+ NADH
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     oxidation-reduction process   5 terms 
  Biochemical function     catalytic activity     5 terms