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PDBsum entry 1wnh

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protein links
Hydrolase inhibitor PDB id
1wnh
Jmol
Contents
Protein chain
220 a.a. *
Waters ×186
* Residue conservation analysis
PDB id:
1wnh
Name: Hydrolase inhibitor
Title: Crystal structure of mouse latexin (tissue carboxypeptidase inhibitor)
Structure: Latexin. Chain: a. Synonym: carboxypeptidase inhibitor. Engineered: yes
Source: Mus musculus. House mouse. Organism_taxid: 10090. Gene: lxn. Expressed in: escherichia coli. Expression_system_taxid: 562.
Biol. unit: Dimer (from PQS)
Resolution:
1.83Å     R-factor:   0.211     R-free:   0.237
Authors: A.Aagaard,P.Listwan,N.Cowieson,T.Huber,T.Ravasi,C.A.Wells, J.U.Flanagan,D.A.Hume,B.Kobe,J.L.Martin
Key ref:
A.Aagaard et al. (2005). An inflammatory role for the mammalian carboxypeptidase inhibitor latexin: relationship to cystatins and the tumor suppressor TIG1. Structure, 13, 309-317. PubMed id: 15698574 DOI: 10.1016/j.str.2004.12.013
Date:
04-Aug-04     Release date:   15-Feb-05    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
P70202  (LXN_MOUSE) -  Latexin
Seq:
Struc:
222 a.a.
220 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     cytoplasm   2 terms 
  Biological process     inflammatory response   4 terms 
  Biochemical function     enzyme inhibitor activity     4 terms  

 

 
DOI no: 10.1016/j.str.2004.12.013 Structure 13:309-317 (2005)
PubMed id: 15698574  
 
 
An inflammatory role for the mammalian carboxypeptidase inhibitor latexin: relationship to cystatins and the tumor suppressor TIG1.
A.Aagaard, P.Listwan, N.Cowieson, T.Huber, T.Ravasi, C.A.Wells, J.U.Flanagan, S.Kellie, D.A.Hume, B.Kobe, J.L.Martin.
 
  ABSTRACT  
 
Latexin, the only known mammalian carboxypeptidase inhibitor, has no detectable sequence similarity with plant and parasite inhibitors, but it is related to a human putative tumor suppressor protein, TIG1. Latexin is expressed in the developing brain, and we find that it plays a role in inflammation, as it is expressed at high levels and is inducible in macrophages in concert with other protease inhibitors and potential protease targets. The crystal structure of mouse latexin, solved at 1.83 A resolution, shows no structural relationship with other carboxypeptidase inhibitors. Furthermore, despite a lack of detectable sequence duplication, the structure incorporates two topologically analogous domains related by pseudo two-fold symmetry. Surprisingly, these domains share a cystatin fold architecture found in proteins that inhibit cysteine proteases, suggesting an evolutionary and possibly functional relationship. The structure of the tumor suppressor protein TIG1 was modeled, revealing its putative membrane binding surface.
 
  Selected figure(s)  
 
Figure 4.
Figure 4. Diversity and Similarity in CP Inhibitors, Cystatin, and Monellin
(A-F) Structures of CP inhibitors from (A) potato (4CPA [Rees and Lipscomb, 1982]) and (B) leech (1DTV [Reverter et al., 2000]) are very different from the structure of mammalian CP inhibitor, (C) latexin. However, latexin (N-terminal domain, [D]) is structurally related to proteins in the (E) cystatin (cysteine protease inhibitor, chicken cystatin) (1CEW [Bode et al., 1988]) and (F) monellin (sweet-tasting protein) (1MOL [Somoza et al., 1993]) fold family.
 
  The above figure is reprinted by permission from Cell Press: Structure (2005, 13, 309-317) copyright 2005.  
  Figure was selected by the author.  

Literature references that cite this PDB file's key reference

  PubMed id Reference
21466706 Y.Li, Z.Basang, H.Ding, Z.Lu, T.Ning, H.Wei, H.Cai, and Y.Ke (2011).
Latexin expression is downregulated in human gastric carcinomas and exhibits tumor suppressor potential.
  BMC Cancer, 11, 121.  
20525390 E.Pérez, J.L.Gallegos, L.Cortés, K.G.Calderón, J.C.Luna, F.E.Cázares, M.C.Velasquillo, J.B.Kouri, and F.C.Hernández (2010).
Identification of latexin by a proteomic analysis in rat normal articular cartilage.
  Proteome Sci, 8, 27.  
19919722 D.Kordis, and V.Turk (2009).
Phylogenomic analysis of the cystatin superfamily in eukaryotes and prokaryotes.
  BMC Evol Biol, 9, 266.  
19924245 G.J.King, K.E.Chen, G.Robin, J.K.Forwood, B.Heras, A.S.Thakur, B.Kobe, S.P.Blomberg, and J.L.Martin (2009).
Interaction between plate make and protein in protein crystallisation screening.
  PLoS One, 4, e7851.  
19796245 G.Van Zant, and Y.Liang (2009).
Natural genetic diversity as a means to uncover stem cell regulatory pathways.
  Ann N Y Acad Sci, 1176, 170-177.  
19452125 M.L.Rose, and M.T.Hincke (2009).
Protein constituents of the eggshell: eggshell-specific matrix proteins.
  Cell Mol Life Sci, 66, 2707-2719.  
17894344 N.P.Cowieson, A.J.Miles, G.Robin, J.K.Forwood, B.Kobe, J.L.Martin, and B.A.Wallace (2008).
Evaluating protein:protein complex formation using synchrotron radiation circular dichroism spectroscopy.
  Proteins, 70, 1142-1146.  
18374916 Y.Liang, and G.Van Zant (2008).
Aging stem cells, latexin, and longevity.
  Exp Cell Res, 314, 1962-1972.  
17996039 I.Pallarés, C.Berenguer, F.X.Avilés, J.Vendrell, and S.Ventura (2007).
Self-assembly of human latexin into amyloid-like oligomers.
  BMC Struct Biol, 7, 75.  
17152080 R.Koike, K.Kinoshita, and A.Kidera (2007).
Probabilistic alignment detects remote homology in a pair of protein sequences without homologous sequence information.
  Proteins, 66, 655-663.  
17220891 Y.Liang, M.Jansen, B.Aronow, H.Geiger, and G.Van Zant (2007).
The quantitative trait gene latexin influences the size of the hematopoietic stem cell population in mice.
  Nat Genet, 39, 178-188.  
15893421 D.Keppler (2006).
Towards novel anti-cancer strategies based on cystatin function.
  Cancer Lett, 235, 159-176.  
17112720 P.R.Mittl, and M.G.Grütter (2006).
Opportunities for structure-based design of protease-directed drugs.
  Curr Opin Struct Biol, 16, 769-775.  
16556043 D.Keppler, and F.Sierra (2005).
Role of cystatins in tumor neovascularization.
  Future Oncol, 1, 661-672.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time.