PDBsum entry 1vra

Transferase

PDB id: 1vra

Contents

Protein chains

191 a.a. *

213 a.a. *

Ligands

SO4 ×2

UNL

EDO ×16

Waters ×390

* Residue conservation analysis

PDB id:

1vra

Name:

Transferase

Title:

Crystal structure of arginine biosynthesis bifunctional protein argj (10175521) from bacillus halodurans at 2.00 a resolution

Structure:

Arginine biosynthesis bifunctional protein argj. Chain: a. Fragment: alpha chain, residues 1-196. Engineered: yes. Arginine biosynthesis bifunctional protein argj. Chain: b. Fragment: beta chain, residues 197-411. Engineered: yes

Source:

Bacillus halodurans. Organism_taxid: 86665. Gene: argj. Expressed in: escherichia coli. Expression_system_taxid: 562. Other_details: both chain a and chain b were expressed from a single construct which encodes residues 1-411 of the argj gene. Construct which encodes residues 1-411 of the argj gene

Biol. unit:

Dimer (from PQS)

Resolution:

2.00Å R-factor: 0.141 R-free: 0.158

Authors:

Joint Center For Structural Genomics (Jcsg)

Key ref:

Joint center for structural genomics (jcsg) Crystal structure of arginine biosynthesis bifunctional protein argj (10175521) from bacillus halodurans at 2.00 a resolution. To be published, .

Date:

17-Feb-05 Release date: 12-Apr-05

Protein chain

Q9K8V3  (ARGJ_BACHD) -  Arginine biosynthesis bifunctional protein ArgJ from Halalkalibacterium halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)

Seq: 411 a.a.

Struc: 191 a.a.

Protein chain

Q9K8V3  (ARGJ_BACHD) -  Arginine biosynthesis bifunctional protein ArgJ from Halalkalibacterium halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)

Seq: 411 a.a.

Struc: 213 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class 1: Chains A, B: E.C.2.3.1.1 - amino-acid N-acetyltransferase.
• Pathway: Ornithine Biosynthesis
• Reaction: L-glutamate + acetyl-CoA = N-acetyl-L-glutamate + CoA + H⁺
• Enzyme class 2: Chains A, B: E.C.2.3.1.35 - glutamate N-acetyltransferase.
• Reaction: N₂-acetyl-L-ornithine + L-glutamate = N-acetyl-L-glutamate + L-ornithine

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site