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PDBsum entry 1vqr

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protein Protein-protein interface(s) links
Unknown function PDB id
1vqr
Jmol
Contents
Protein chains
284 a.a. *
269 a.a. *
Waters ×193
* Residue conservation analysis
PDB id:
1vqr
Name: Unknown function
Title: Crystal structure of a virulence factor (cj0248) from campyl jejuni subsp. Jejuni at 2.25 a resolution
Structure: Hypothetical protein cj0248. Chain: a, b, c, d. Engineered: yes
Source: Campylobacter jejuni subsp. Jejuni nct organism_taxid: 192222. Strain: subsp. Jejuni nctc 11168. Gene: cj0248. Expressed in: escherichia coli. Expression_system_taxid: 562.
Biol. unit: Tetramer (from PQS)
Resolution:
2.25Å     R-factor:   0.195     R-free:   0.233
Authors: Joint Center For Structural Genomics (Jcsg)
Key ref:
Q.Xu et al. (2006). Crystal structure of virulence factor CJ0248 from Campylobacter jejuni at 2.25 A resolution reveals a new fold. Proteins, 62, 292-296. PubMed id: 16287129 DOI: 10.1002/prot.20611
Date:
17-Dec-04     Release date:   28-Dec-04    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
Q0PBQ7  (Q0PBQ7_CAMJE) -  Putative uncharacterized protein
Seq:
Struc:
285 a.a.
284 a.a.
Protein chains
Pfam   ArchSchema ?
Q0PBQ7  (Q0PBQ7_CAMJE) -  Putative uncharacterized protein
Seq:
Struc:
285 a.a.
269 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 

 
DOI no: 10.1002/prot.20611 Proteins 62:292-296 (2006)
PubMed id: 16287129  
 
 
Crystal structure of virulence factor CJ0248 from Campylobacter jejuni at 2.25 A resolution reveals a new fold.
Q.Xu, R.Schwarzenbacher, D.McMullan, P.Abdubek, S.Agarwalla, E.Ambing, H.Axelrod, T.Biorac, J.M.Canaves, H.J.Chiu, A.M.Deacon, M.DiDonato, M.A.Elsliger, A.Godzik, C.Grittini, S.K.Grzechnik, J.Hale, E.Hampton, G.W.Han, J.Haugen, M.Hornsby, L.Jaroszewski, H.E.Klock, E.Koesema, A.Kreusch, P.Kuhn, S.A.Lesley, M.D.Miller, K.Moy, E.Nigoghossian, J.Paulsen, K.Quijano, R.Reyes, C.Rife, G.Spraggon, R.C.Stevens, H.van den Bedem, J.Velasquez, A.White, G.Wolf, K.O.Hodgson, J.Wooley, I.A.Wilson.
 
  ABSTRACT  
 
No abstract given.

 
  Selected figure(s)  
 
Figure 1.
Figure 1. Crystal structure of CJ0248 from Campylobacter jejuni. (A) Ribbon diagram of CJ0248 color-coded from N-terminus (blue) to C-terminus (red) showing the domain organization. Helices H1-H19 are indicated. (B) Diagram showing the secondary structure elements in CJ0248 superimposed on its primary sequence. The -helices, -bulges, and disordered regions (dots) are indicated for chain A.
Figure 2.
Figure 2. (A) CJ0248 in surface representation colored according to electrostatic potential in a range where red is negative (-75 kT/e) and blue is positive (+75 kT/e). Note the deep pocket guarded by a negatively charged entrance. (B) Superposition of CJ0248 (blue ribbon; same view as A) with the catalytic core domain (residues 230-345 and 377-393) of human phosphodiesterase 4B2B (PDB: 1f0j; yellow ribbon with zinc depicted as yellow sphere). (C) Same view as B, but zoomed into the active site of human phosphodiesterase with zinc (purple sphere) and coordinating residues shown in white sticks. CJ0248 residues in the postulated active site are shown in blue sticks and labeled in bold. The HD motif (His274 and Asp275 versus Leu146 and Arg147) and zinc center are not conserved in CJ0248.
 
  The above figures are reprinted by permission from John Wiley & Sons, Inc.: Proteins (2006, 62, 292-296) copyright 2006.