PDBsum entry 1vom

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Muscle protein PDB id
Protein chain
730 a.a. *
Waters ×705
* Residue conservation analysis
PDB id:
Name: Muscle protein
Title: Complex between dictyostelium myosin and mgadp and vanadate at 1.9a resolution
Structure: Myosin. Chain: a. Fragment: truncated at residue 762. Engineered: yes. Other_details: this molecule was truncated to yield a fragment that crystallizes readily
Source: Dictyostelium discoideum. Organism_taxid: 44689. Expressed in: dictyostelium discoideum. Expression_system_taxid: 44689
1.90Å     R-factor:   0.194    
Authors: I.Rayment,C.A.Smith
Key ref:
C.A.Smith and I.Rayment (1996). X-ray structure of the magnesium(II).ADP.vanadate complex of the Dictyostelium discoideum myosin motor domain to 1.9 A resolution. Biochemistry, 35, 5404-5417. PubMed id: 8611530 DOI: 10.1021/bi952633+
09-Nov-95     Release date:   23-Dec-96    
Go to PROCHECK summary

Protein chain
Pfam   ArchSchema ?
P08799  (MYS2_DICDI) -  Myosin-2 heavy chain
2116 a.a.
730 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 7 residue positions (black crosses)

 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     myosin complex   1 term 
  Biochemical function     ATP binding     2 terms  


DOI no: 10.1021/bi952633+ Biochemistry 35:5404-5417 (1996)
PubMed id: 8611530  
X-ray structure of the magnesium(II).ADP.vanadate complex of the Dictyostelium discoideum myosin motor domain to 1.9 A resolution.
C.A.Smith, I.Rayment.
The structure of the vanadate-trapped ADP complex of a truncated head of Dictyostelium myosin II consisting of residues Asp 2-Asn 762 has been determined by molecular replacement at 1.9 A resolution and refined to a crystallographic R-factor of 19.4%. The crystals belong to the orthorhombic space group C2221 where a = 84.50 A, b = 145.4 A, and c = 152.8 A. The conformation of the protein is similar to that of MgADP.AlF4.SlDc [Fisher, A.J., et al. (1995) Biochemistry 34, 8960-8972]. The nucleotide binding site contains a complex between MgADP and vanadate where MgADP exhibits a very similar conformation to that seen in previous complexes. The vanadate ion adopts a trigonal bipyramidal coordination. The three equatorial oxygen ligands are fairly short, average 1.7 A, relative to a single bond distance of approximately 1.8 A and are coordinated to the magnesium ion, N zeta of Lys 185, and five other protein ligands. The apical coordination to the vanadate ion is filled by a terminal oxygen on the beta-phosphate of ADP and a water molecule at bond distances of 2.1 and 2.3 A, respectively. The long length of the apical bonds suggests that the bond order is considerably less than unity. This structure confirms the earlier suggestion that vanadate is a model for the transition state of ATP hydrolysis and thus provides insight into those factors that are responsible for catalysis. In particular, it shows that the protein ligands and water structure surrounding the gamma-phosphate pocket are oriented to stabilize a water molecule in an appropriate position for in-line nucleophilic attack on the gamma-phosphorus of ATP. This structure reveals also an orientation of the COOH-terminal region beyond Thr 688 which is very different from that observed in either MgADP.BeFx.SlDc or chicken skeletal myosin subfragment 1. This is consistent with the COOH-terminal region of the molecule playing an important role in the transduction of chemical energy of hydrolysis of ATP into mechanical movement.

Literature references that cite this PDB file's key reference

  PubMed id Reference
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Conformational changes induced by ATP-hydrolysis in an ABC transporter: A molecular dynamics study of the Sav1866 exporter.
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The Transition-Like State and P(i) Entrance into the Catalytic A Subunit of the Biological Engine A-ATP Synthase.
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PDB codes: 3nd8 3nd9 3p20
21277856 N.Naber, A.Larson, S.Rice, R.Cooke, and E.Pate (2011).
Multiple conformations of the nucleotide site of Kinesin family motors in the triphosphate state.
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20018897 C.L.Parke, E.J.Wojcik, S.Kim, and D.K.Worthylake (2010).
ATP hydrolysis in Eg5 kinesin involves a catalytic two-water mechanism.
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PDB code: 3hqd
20164408 F.Fourniol, and C.A.Moores (2010).
Kinesin motor activation: microtubules pull the switches.
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21132353 G.Offer, and K.W.Ranatunga (2010).
Crossbridge and filament compliance in muscle: implications for tension generation and lever arm swing.
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20050614 H.H.Nguyen, L.Wang, H.Huang, E.Peisach, D.Dunaway-Mariano, and K.N.Allen (2010).
Structural determinants of substrate recognition in the HAD superfamily member D-glycero-D-manno-heptose-1,7-bisphosphate phosphatase (GmhB) .
  Biochemistry, 49, 1082-1092.
PDB codes: 3l8e 3l8f 3l8g 3l8h
20459085 J.J.Frye, V.A.Klenchin, C.R.Bagshaw, and I.Rayment (2010).
Insights into the importance of hydrogen bonding in the gamma-phosphate binding pocket of myosin: structural and functional studies of serine 236.
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PDB codes: 3myh 3myk 3myl
20399183 M.Cecchini, Y.Alexeev, and M.Karplus (2010).
Pi release from myosin: a simulation analysis of possible pathways.
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20194770 R.Elber, and A.West (2010).
Atomically detailed simulation of the recovery stroke in myosin by Milestoning.
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Electron tomography of cryofixed, isometrically contracting insect flight muscle reveals novel actin-myosin interactions.
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PDB codes: 2w49 2w4a 2w4g 2w4t
19804726 D.Hyatt, R.Cooke, and E.Pate (2009).
Analysis of the interaction of the nucleotide base with myosin and the effect on substrate efficacy.
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19008235 E.Forgacs, T.Sakamoto, S.Cartwright, B.Belknap, M.Kovács, J.Tóth, M.R.Webb, J.R.Sellers, and H.D.White (2009).
Switch 1 Mutation S217A Converts Myosin V into a Low Duty Ratio Motor.
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19581439 H.Onishi (2009).
Yuji Tonomura: a pioneer in the field of energy transduction in muscle contraction.
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19125340 M.Stewart, K.Franks-Skiba, and R.Cooke (2009).
Myosin regulatory light chain phosphorylation inhibits shortening velocities of skeletal muscle fibers in the presence of the myosin inhibitor blebbistatin.
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19966224 R.V.Agafonov, I.V.Negrashov, Y.V.Tkachev, S.E.Blakely, M.A.Titus, D.D.Thomas, and Y.E.Nesmelov (2009).
Structural dynamics of the myosin relay helix by time-resolved EPR and FRET.
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19413972 S.Xu, H.D.White, G.W.Offer, and L.C.Yu (2009).
Stabilization of helical order in the thick filaments by blebbistatin: further evidence of coexisting multiple conformations of myosin.
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19292432 Y.Yang, and Q.Cui (2009).
Does water relay play an important role in phosphoryl transfer reactions? Insights from theoretical study of a model reaction in water and tert-butanol.
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19534504 Y.Yang, and Q.Cui (2009).
The hydrolysis activity of adenosine triphosphate in myosin: a theoretical analysis of anomeric effects and the nature of the transition state.
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19756531 Z.Song, K.J.Parker, I.Enoh, H.Zhao, and O.Olubajo (2009).
Myosin-catalyzed ATP hydrolysis elucidated by 31P NMR kinetic studies and 1H PFG-diffusion measurements.
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18528569 C.Lucas-Lopez, J.S.Allingham, T.Lebl, C.P.Lawson, R.Brenk, J.R.Sellers, I.Rayment, and N.J.Westwood (2008).
The small molecule tool (S)-(-)-blebbistatin: novel insights of relevance to myosin inhibitor design.
  Org Biomol Chem, 6, 2076-2084.
PDB codes: 3bz7 3bz8 3bz9
17981900 C.M.Yengo, S.K.Ananthanarayanan, C.A.Brosey, S.Mao, and M.J.Tyska (2008).
Human deafness mutation E385D disrupts the mechanochemical coupling and subcellular targeting of myosin-1a.
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19008861 D.Luo, T.Xu, R.P.Watson, D.Scherer-Becker, A.Sampath, W.Jahnke, S.S.Yeong, C.H.Wang, S.P.Lim, A.Strongin, S.G.Vasudevan, and J.Lescar (2008).
Insights into RNA unwinding and ATP hydrolysis by the flavivirus NS3 protein.
  EMBO J, 27, 3209-3219.
PDB codes: 2jlq 2jlr 2jls 2jlu 2jlv 2jlw 2jlx 2jly 2jlz
19325727 I.Aprodu, A.Redaelli, and M.Soncini (2008).
Actomyosin interaction: mechanical and energetic properties in different nucleotide binding States.
  Int J Mol Sci, 9, 1927-1943.  
18725645 J.C.Klein, A.R.Burr, B.Svensson, D.J.Kennedy, J.Allingham, M.A.Titus, I.Rayment, and D.D.Thomas (2008).
Actin-binding cleft closure in myosin II probed by site-directed spin labeling and pulsed EPR.
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18568345 M.J.Harris, and H.J.Woo (2008).
Energetics of subdomain movements and fluorescence probe solvation environment change in ATP-bound myosin.
  Eur Biophys J, 38, 1.  
18765799 R.V.Agafonov, Y.E.Nesmelov, M.A.Titus, and D.D.Thomas (2008).
Muscle and nonmuscle myosins probed by a spin label at equivalent sites in the force-generating domain.
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18700726 S.Watanabe, N.Umeki, R.Ikebe, and M.Ikebe (2008).
Impacts of Usher syndrome type IB mutations on human myosin VIIa motor function.
  Biochemistry, 47, 9505-9513.  
18462751 W.A.Kronert, C.M.Dambacher, A.F.Knowles, D.M.Swank, and S.I.Bernstein (2008).
Alternative relay domains of Drosophila melanogaster myosin differentially affect ATPase activity, in vitro motility, myofibril structure and muscle function.
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18216256 X.D.Li, H.S.Jung, Q.Wang, R.Ikebe, R.Craig, and M.Ikebe (2008).
The globular tail domain puts on the brake to stop the ATPase cycle of myosin Va.
  Proc Natl Acad Sci U S A, 105, 1140-1145.  
18339764 Y.E.Nesmelov, R.V.Agafonov, A.R.Burr, R.T.Weber, and D.D.Thomas (2008).
Structure and dynamics of the force-generating domain of myosin probed by multifrequency electron paramagnetic resonance.
  Biophys J, 95, 247-256.  
18619975 Y.Yang, H.Yu, and Q.Cui (2008).
Extensive conformational transitions are required to turn on ATP hydrolysis in myosin.
  J Mol Biol, 381, 1407-1420.  
17449872 A.Málnási-Csizmadia, J.Tóth, D.S.Pearson, C.Hetényi, L.Nyitray, M.A.Geeves, C.R.Bagshaw, and M.Kovács (2007).
Selective perturbation of the myosin recovery stroke by point mutations at the base of the lever arm affects ATP hydrolysis and phosphate release.
  J Biol Chem, 282, 17658-17664.  
17488874 A.T.Torelli, J.Krucinska, and J.E.Wedekind (2007).
A comparison of vanadate to a 2'-5' linkage at the active site of a small ribozyme suggests a role for water in transition-state stabilization.
  RNA, 13, 1052-1070.
PDB codes: 2p7d 2p7e 2p7f
18024585 A.Ward, C.L.Reyes, J.Yu, C.B.Roth, and G.Chang (2007).
Flexibility in the ABC transporter MsbA: Alternating access with a twist.
  Proc Natl Acad Sci U S A, 104, 19005-19010.
PDB codes: 3b5w 3b5x 3b5y 3b5z 3b60
17704146 F.Takagi, and M.Kikuchi (2007).
Structural change and nucleotide dissociation of Myosin motor domain: dual go model simulation.
  Biophys J, 93, 3820-3827.  
17717642 G.Tünnemann, P.Karczewski, H.Haase, M.C.Cardoso, and I.Morano (2007).
Modulation of muscle contraction by a cell-permeable peptide.
  J Mol Med, 85, 1405-1412.  
17384472 H.Kagawa (2007).
Quantum chemical studies of the myosin ATPase mechanism.
  J Nippon Med Sch, 74, 4.  
17640901 H.Onishi, and M.F.Morales (2007).
A closer look at energy transduction in muscle.
  Proc Natl Acad Sci U S A, 104, 12714-12719.  
17291159 H.Yu, L.Ma, Y.Yang, and Q.Cui (2007).
Mechanochemical coupling in the myosin motor domain. I. Insights from equilibrium active-site simulations.
  PLoS Comput Biol, 3, e21.  
17305418 H.Yu, L.Ma, Y.Yang, and Q.Cui (2007).
Mechanochemical coupling in the myosin motor domain. II. Analysis of critical residues.
  PLoS Comput Biol, 3, e23.  
17956731 J.Ménétrey, P.Llinas, M.Mukherjea, H.L.Sweeney, and A.Houdusse (2007).
The structural basis for the large powerstroke of myosin VI.
  Cell, 131, 300-308.
PDB code: 2v26
17704147 M.F.Halstead, K.Ajtai, A.R.Penheiter, J.D.Spencer, Y.Zheng, E.A.Morrison, and T.P.Burghardt (2007).
An unusual transduction pathway in human tonic smooth muscle myosin.
  Biophys J, 93, 3555-3566.  
17164240 M.Herget, G.Oancea, S.Schrodt, M.Karas, R.Tampé, and R.Abele (2007).
Mechanism of substrate sensing and signal transmission within an ABC transporter: use of a Trojan horse strategy.
  J Biol Chem, 282, 3871-3880.  
17485460 P.M.Jones, and A.M.George (2007).
Nucleotide-dependent allostery within the ABC transporter ATP-binding cassette: a computational study of the MJ0796 dimer.
  J Biol Chem, 282, 22793-22803.  
17900617 S.Tang, J.C.Liao, A.R.Dunn, R.B.Altman, J.A.Spudich, and J.P.Schmidt (2007).
Predicting allosteric communication in myosin via a pathway of conserved residues.
  J Mol Biol, 373, 1361-1373.  
17913331 T.P.Burghardt, J.Y.Hu, and K.Ajtai (2007).
Myosin dynamics on the millisecond time scale.
  Biophys Chem, 131, 15-28.  
17596847 W.Zheng, B.R.Brooks, and G.Hummer (2007).
Protein conformational transitions explored by mixed elastic network models.
  Proteins, 69, 43-57.  
18158894 Y.Sun, H.W.Schroeder, J.F.Beausang, K.Homma, M.Ikebe, and Y.E.Goldman (2007).
Myosin VI walks "wiggly" on actin with large and variable tilting.
  Mol Cell, 28, 954-964.  
16470332 B.Brenner (2006).
The stroke size of myosins: a reevaluation.
  J Muscle Res Cell Motil, 27, 173-187.  
16627949 C.A.Smith, J.A.Cross, A.L.Bognar, and X.Sun (2006).
Mutation of Gly51 to serine in the P-loop of Lactobacillus casei folylpolyglutamate synthetase abolishes activity by altering the conformation of two adjacent loops.
  Acta Crystallogr D Biol Crystallogr, 62, 548-558.
PDB codes: 2gc5 2gc6 2gca 2gcb
16531409 C.Chen, A.K.Saxena, W.N.Simcoke, D.N.Garboczi, P.L.Pedersen, and Y.H.Ko (2006).
Mitochondrial ATP synthase. Crystal structure of the catalytic F1 unit in a vanadate-induced transition-like state and implications for mechanism.
  J Biol Chem, 281, 13777-13783.
PDB code: 2f43
16541253 C.Oswald, I.B.Holland, and L.Schmitt (2006).
The motor domains of ABC-transporters. What can structures tell us?
  Naunyn Schmiedebergs Arch Pharmacol, 372, 385-399.  
16352607 D.W.Zhang, G.A.Graf, R.D.Gerard, J.C.Cohen, and H.H.Hobbs (2006).
Functional asymmetry of nucleotide-binding domains in ABCG5 and ABCG8.
  J Biol Chem, 281, 4507-4516.  
17215884 F.J.Sharom (2006).
Shedding light on drug transport: structure and function of the P-glycoprotein multidrug transporter (ABCB1).
  Biochem Cell Biol, 84, 979-992.  
16603626 H.Onishi, S.V.Mikhailenko, and M.F.Morales (2006).
Toward understanding actin activation of myosin ATPase: the role of myosin surface loops.
  Proc Natl Acad Sci U S A, 103, 6136-6141.  
16867993 J.Wang, F.Sun, D.W.Zhang, Y.Ma, F.Xu, J.D.Belani, J.C.Cohen, H.H.Hobbs, and X.S.Xie (2006).
Sterol transfer by ABCG5 and ABCG8: in vitro assay and reconstitution.
  J Biol Chem, 281, 27894-27904.  
17154545 L.Shaner, R.Sousa, and K.A.Morano (2006).
Characterization of Hsp70 binding and nucleotide exchange by the yeast Hsp110 chaperone Sse1.
  Biochemistry, 45, 15075-15084.  
16361336 P.Petrone, and V.S.Pande (2006).
Can conformational change be described by only a few normal modes?
  Biophys J, 90, 1583-1593.  
16982629 S.Fujita-Becker, G.Tsiavaliaris, R.Ohkura, T.Shimada, D.J.Manstein, and K.Sutoh (2006).
Functional characterization of the N-terminal region of myosin-2.
  J Biol Chem, 281, 36102-36109.  
16450056 S.Fujita-Becker, T.F.Reubold, and K.C.Holmes (2006).
The actin-binding cleft: functional characterisation of myosin II with a strut mutation.
  J Muscle Res Cell Motil, 27, 115-123.  
16617427 S.Mesentean, S.Fischer, and J.C.Smith (2006).
Analyzing large-scale structural change in proteins: comparison of principal component projection and Sammon mapping.
  Proteins, 64, 210-218.  
16645962 Y.Liu, M.Scolari, W.Im, and H.J.Woo (2006).
Protein-protein interactions in actin-myosin binding and structural effects of R405Q mutation: a molecular dynamics study.
  Proteins, 64, 156-166.  
16844693 Z.E.Sauna, K.Nandigama, and S.V.Ambudkar (2006).
Exploiting reaction intermediates of the ATPase reaction to elucidate the mechanism of transport by P-glycoprotein (ABCB1).
  J Biol Chem, 281, 26501-26511.  
16075160 A.Málnási-Csizmadia, J.L.Dickens, W.Zeng, and C.R.Bagshaw (2005).
Switch movements and the myosin crossbridge stroke.
  J Muscle Res Cell Motil, 26, 31-37.  
15890884 C.L.Reyes, and G.Chang (2005).
Structure of the ABC transporter MsbA in complex with ADP.vanadate and lipopolysaccharide.
  Science, 308, 1028-1031.
PDB code: 1z2r
16691487 F.J.Sharom, M.R.Lugo, and P.D.Eckford (2005).
New insights into the drug binding, transport and lipid flippase activities of the p-glycoprotein multidrug transporter.
  J Bioenerg Biomembr, 37, 481-487.  
16006554 J.B.Thoden, and H.M.Holden (2005).
The molecular architecture of human N-acetylgalactosamine kinase.
  J Biol Chem, 280, 32784-32791.
PDB codes: 2a2c 2a2d
15750603 J.S.Allingham, R.Smith, and I.Rayment (2005).
The structural basis of blebbistatin inhibition and specificity for myosin II.
  Nat Struct Mol Biol, 12, 378-379.
PDB code: 1yv3
15642268 M.A.Ferenczi, S.Y.Bershitsky, N.Koubassova, V.Siththanandan, W.I.Helsby, P.Panine, M.Roessle, T.Narayanan, and A.K.Tsaturyan (2005).
The "roll and lock" mechanism of force generation in muscle.
  Structure, 13, 131-141.  
16118217 S.D.Auerbach, and K.A.Johnson (2005).
Kinetic effects of kinesin switch I and switch II mutations.
  J Biol Chem, 280, 37061-37068.  
15863618 S.Fischer, B.Windshügel, D.Horak, K.C.Holmes, and J.C.Smith (2005).
Structural mechanism of the recovery stroke in the myosin molecular motor.
  Proc Natl Acad Sci U S A, 102, 6873-6878.  
15579901 S.S.Rosenfeld, A.Houdusse, and H.L.Sweeney (2005).
Magnesium regulates ADP dissociation from myosin V.
  J Biol Chem, 280, 6072-6079.  
15189142 A.L.Davidson, and J.Chen (2004).
ATP-binding cassette transporters in bacteria.
  Annu Rev Biochem, 73, 241-268.  
14691941 A.R.Ferré-D'Amaré (2004).
The hairpin ribozyme.
  Biopolymers, 73, 71-78.  
15454448 C.Karatzaferi, M.K.Chinn, and R.Cooke (2004).
The force exerted by a muscle cross-bridge depends directly on the strength of the actomyosin bond.
  Biophys J, 87, 2532-2544.  
15627371 D.I.Levitsky (2004).
Actomyosin systems of biological motility.
  Biochemistry (Mosc), 69, 1177-1189.  
15070725 D.J.Crampton, S.Guo, D.E.Johnson, and C.C.Richardson (2004).
The arginine finger of bacteriophage T7 gene 4 helicase: role in energy coupling.
  Proc Natl Acad Sci U S A, 101, 4373-4378.  
15630611 D.M.Warshaw (2004).
Lever arms and necks: a common mechanistic theme across the myosin superfamily.
  J Muscle Res Cell Motil, 25, 467-474.  
15184651 D.Risal, S.Gourinath, D.M.Himmel, A.G.Szent-Györgyi, and C.Cohen (2004).
Myosin subfragment 1 structures reveal a partially bound nucleotide and a complex salt bridge that helps couple nucleotide and actin binding.
  Proc Natl Acad Sci U S A, 101, 8930-8935.
PDB codes: 1s5g 1sr6
14747312 G.Li, and Q.Cui (2004).
Analysis of functional motions in Brownian molecular machines with an efficient block normal mode approach: myosin-II and Ca2+ -ATPase.
  Biophys J, 86, 743-763.  
15159388 G.Tombline, L.A.Bartholomew, I.L.Urbatsch, and A.E.Senior (2004).
Combined mutation of catalytic glutamate residues in the two nucleotide binding domains of P-glycoprotein generates a conformation that binds ATP and ADP tightly.
  J Biol Chem, 279, 31212-31220.  
14638679 G.Tombline, L.Bartholomew, K.Gimi, G.A.Tyndall, and A.E.Senior (2004).
Synergy between conserved ABC signature Ser residues in P-glycoprotein catalysis.
  J Biol Chem, 279, 5363-5373.  
15189875 J.D.Lawson, E.Pate, I.Rayment, and R.G.Yount (2004).
Molecular dynamics analysis of structural factors influencing back door pi release in myosin.
  Biophys J, 86, 3794-3803.  
15711883 J.Van Dijk, C.Lafont, M.L.Knetsch, J.Derancourt, D.J.Manstein, E.C.Long, and P.Chaussepied (2004).
Conformational changes in actin-myosin isoforms probed by Ni(II).Gly-Gly-His reactivity.
  J Muscle Res Cell Motil, 25, 527-537.  
15020589 K.Ajtai, S.P.Garamszegi, S.Watanabe, M.Ikebe, and T.P.Burghardt (2004).
The myosin cardiac loop participates functionally in the actomyosin interaction.
  J Biol Chem, 279, 23415-23421.  
15647158 K.C.Holmes, R.R.Schröder, H.L.Sweeney, and A.Houdusse (2004).
The structure of the rigor complex and its implications for the power stroke.
  Philos Trans R Soc Lond B Biol Sci, 359, 1819-1828.  
15117946 M.I.Austermuhle, J.A.Hall, C.S.Klug, and A.L.Davidson (2004).
Maltose-binding protein is open in the catalytic transition state for ATP hydrolysis during maltose transport.
  J Biol Chem, 279, 28243-28250.  
15146428 P.B.Furspan, S.Chatterjee, and R.R.Freedman (2004).
Increased tyrosine phosphorylation mediates the cooling-induced contraction and increased vascular reactivity of Raynaud's disease.
  Arthritis Rheum, 50, 1578-1585.  
15510214 P.D.Coureux, H.L.Sweeney, and A.Houdusse (2004).
Three myosin V structures delineate essential features of chemo-mechanical transduction.
  EMBO J, 23, 4527-4537.
PDB codes: 1w7i 1w7j 1w8j
15292263 P.P.Chakrabarti, Y.Suveyzdis, A.Wittinghofer, and K.Gerwert (2004).
Fourier transform infrared spectroscopy on the Rap.RapGAP reaction, GTPase activation without an arginine finger.
  J Biol Chem, 279, 46226-46233.  
15286375 R.Nitta, M.Kikkawa, Y.Okada, and N.Hirokawa (2004).
KIF1A alternately uses two loops to bind microtubules.
  Science, 305, 678-683.
PDB codes: 1vfv 1vfw 1vfx 1vfz
14754887 S.Danko, K.Yamasaki, T.Daiho, and H.Suzuki (2004).
Distinct natures of beryllium fluoride-bound, aluminum fluoride-bound, and magnesium fluoride-bound stable analogues of an ADP-insensitive phosphoenzyme intermediate of sarcoplasmic reticulum Ca2+-ATPase: changes in catalytic and transport sites during phosphoenzyme hydrolysis.
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15647160 W.Zeng, P.B.Conibear, J.L.Dickens, R.A.Cowie, S.Wakelin, A.Málnási-Csizmadia, and C.R.Bagshaw (2004).
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Probing the conformational states of the SH1-SH2 helix in myosin: a cross-linking approach.
  Biochemistry, 37, 16704-16710.  
9790677 L.Moschcovich, Y.M.Peyser, C.Salomon, T.P.Burghardt, and A.Muhlrad (1998).
Interaction of myosin subfragment 1 with two non-nucleotide ATP analogues.
  Biochemistry, 37, 15137-15143.  
9949764 N.Sasaki, and K.Sutoh (1998).
Structure-mutation analysis of the ATPase site of Dictyostelium discoideum myosin II.
  Adv Biophys, 35, 1.  
9685384 N.Sasaki, T.Shimada, and K.Sutoh (1998).
Mutational analysis of the switch II loop of Dictyostelium myosin II.
  J Biol Chem, 273, 20334-20340.  
9741621 R.Dominguez, Y.Freyzon, K.M.Trybus, and C.Cohen (1998).
Crystal structure of a vertebrate smooth muscle myosin motor domain and its complex with the essential light chain: visualization of the pre-power stroke state.
  Cell, 94, 559-571.
PDB codes: 1br1 1br2 1br4
9585528 T.Hiratsuka (1998).
Prodan fluorescence reflects differences in nucleotide-induced conformational states in the myosin head and allows continuous visualization of the ATPase reactions.
  Biochemistry, 37, 7167-7176.  
9778376 U.S.Rao (1998).
Drug binding and nucleotide hydrolyzability are essential requirements in the vanadate-induced inhibition of the human P-glycoprotein ATPase.
  Biochemistry, 37, 14981-14988.  
9789002 W.Liang, and J.A.Spudich (1998).
Nucleotide-dependent conformational change near the fulcrum region in Dictyostelium myosin II.
  Proc Natl Acad Sci U S A, 95, 12844-12847.  
9675167 W.Wriggers, and K.Schulten (1998).
Nucleotide-dependent movements of the kinesin motor domain predicted by simulated annealing.
  Biophys J, 75, 646-661.  
9765269 X.D.Li, T.E.Rhodes, R.Ikebe, T.Kambara, H.D.White, and M.Ikebe (1998).
Effects of mutations in the gamma-phosphate binding site of myosin on its motor function.
  J Biol Chem, 273, 27404-27411.  
9546383 Y.E.Goldman (1998).
Wag the tail: structural dynamics of actomyosin.
  Cell, 93, 1-4.  
9765259 Y.Kakuta, E.V.Petrotchenko, L.C.Pedersen, and M.Negishi (1998).
The sulfuryl transfer mechanism. Crystal structure of a vanadate complex of estrogen sulfotransferase and mutational analysis.
  J Biol Chem, 273, 27325-27330.
PDB code: 1bo6
9305951 A.M.Gulick, C.B.Bauer, J.B.Thoden, and I.Rayment (1997).
X-ray structures of the MgADP, MgATPgammaS, and MgAMPPNP complexes of the Dictyostelium discoideum myosin motor domain.
  Biochemistry, 36, 11619-11628.
PDB codes: 1mma 1mmg 1mmn
9230689 A.M.Gulick, and I.Rayment (1997).
Structural studies on myosin II: communication between distant protein domains.
  Bioessays, 19, 561-569.  
9057826 B.C.Phan, Y.M.Peyser, E.Reisler, and A.Muhlrad (1997).
Effect of complexes of ADP and phosphate analogs on the conformation of the Cys707-Cys697 region of myosin subfragment 1.
  Eur J Biochem, 243, 636-642.  
9346898 B.Patterson, K.M.Ruppel, Y.Wu, and J.A.Spudich (1997).
Cold-sensitive mutants G680V and G691C of Dictyostelium myosin II confer dramatically different biochemical defects.
  J Biol Chem, 272, 27612-27617.  
9184168 B.Sankaran, S.Bhagat, and A.E.Senior (1997).
Inhibition of P-glycoprotein ATPase activity by beryllium fluoride.
  Biochemistry, 36, 6847-6853.  
9341208 C.Smyczynski, and A.A.Kasprzak (1997).
Effect of nucleotides and actin on the orientation of the light chain-binding domain in myosin subfragment 1.
  Biochemistry, 36, 13201-13207.  
9315852 E.Pate, N.Naber, M.Matuska, K.Franks-Skiba, and R.Cooke (1997).
Opening of the myosin nucleotide triphosphate binding domain during the ATPase cycle.
  Biochemistry, 36, 12155-12166.  
9062359 H.Fujita, S.Sugiura, S.Momomura, M.Omata, H.Sugi, and K.Sutoh (1997).
Characterization of mutant myosins of Dictyostelium discoideum equivalent to human familial hypertrophic cardiomyopathy mutants. Molecular force level of mutant myosins may have a prognostic implication.
  J Clin Invest, 99, 1010-1015.  
9305989 K.Polosukhina, and S.Highsmith (1997).
Kinetic investigation of the ligand dependence of rabbit skeletal muscle myosin subfragment 1 Cys-697 and Cys-707 reactivities.
  Biochemistry, 36, 11952-11958.  
9094328 L.A.Amos, and R.A.Cross (1997).
Structure and dynamics of molecular motors.
  Curr Opin Struct Biol, 7, 239-246.  
8993313 M.Zhang, M.Zhou, R.L.Van Etten, and C.V.Stauffacher (1997).
Crystal structure of bovine low molecular weight phosphotyrosyl phosphatase complexed with the transition state analog vanadate.
  Biochemistry, 36, 15-23.
PDB codes: 1dg9 1z12 1z13
9245400 N.Naber, R.Cooke, and E.Pate (1997).
Binding of ncd to microtubules induces a conformational change near the junction of the motor domain with the neck.
  Biochemistry, 36, 9681-9689.  
9245402 R.Bertrand, J.Derancourt, and R.Kassab (1997).
Probing the hydrophobic interactions in the skeletal actomyosin subfragment 1 and its nucleotide complexes by zero-length cross-linking with a nickel-peptide chelate.
  Biochemistry, 36, 9703-9714.  
9442886 R.D.Vale, and R.J.Fletterick (1997).
The design plan of kinesin motors.
  Annu Rev Cell Dev Biol, 13, 745-777.  
9238011 R.Mendelson, and E.P.Morris (1997).
The structure of the acto-myosin subfragment 1 complex: results of searches using data from electron microscopy and x-ray crystallography.
  Proc Natl Acad Sci U S A, 94, 8533-8538.
PDB code: 1alm
9003183 S.E.Kurzawa, D.J.Manstein, and M.A.Geeves (1997).
Dictyostelium discoideum myosin II: characterization of functional myosin motor fragments.
  Biochemistry, 36, 317-323.  
9116016 S.Park, K.Ajtai, and T.P.Burghardt (1997).
Mechanism for coupling free energy in ATPase to the myosin active site.
  Biochemistry, 36, 3368-3372.  
9275174 T.P.Burghardt, S.P.Garamszegi, and K.Ajtai (1997).
Probes bound to myosin Cys-707 rotate during length transients in contraction.
  Proc Natl Acad Sci U S A, 94, 9631-9636.  
9369475 T.Shimada, N.Sasaki, R.Ohkura, and K.Sutoh (1997).
Alanine scanning mutagenesis of the switch I region in the ATPase site of Dictyostelium discoideum myosin II.
  Biochemistry, 36, 14037-14043.  
9228065 Y.H.Ko, M.Bianchet, L.M.Amzel, and P.L.Pedersen (1997).
Novel insights into the chemical mechanism of ATP synthase. Evidence that in the transition state the gamma-phosphate of ATP is near the conserved alanine within the P-loop of the beta-subunit.
  J Biol Chem, 272, 18875-18881.  
9108019 Y.W.Xu, S.Moréra, J.Janin, and J.Cherfils (1997).
AlF3 mimics the transition state of protein phosphorylation in the crystal structure of nucleoside diphosphate kinase and MgADP.
  Proc Natl Acad Sci U S A, 94, 3579-3583.
PDB codes: 1kdn 2bef
8995355 Y.Z.Ma, and E.W.Taylor (1997).
Kinetic mechanism of a monomeric kinesin construct.
  J Biol Chem, 272, 717-723.  
8968602 C.L.Berger, J.S.Craik, D.R.Trentham, J.E.Corrie, and Y.E.Goldman (1996).
Fluorescence polarization of skeletal muscle fibers labeled with rhodamine isomers on the myosin heavy chain.
  Biophys J, 71, 3330-3343.  
  8663496 I.Rayment (1996).
The structural basis of the myosin ATPase activity.
  J Biol Chem, 271, 15850-15853.  
8736548 I.Rayment (1996).
Kinesin and myosin: molecular motors with similar engines.
  Structure, 4, 501-504.  
8952512 J.C.Grammer, J.A.Loo, C.G.Edmonds, C.R.Cremo, and R.G.Yount (1996).
Chemistry and mechanism of vanadate-promoted photooxidative cleavage of myosin.
  Biochemistry, 35, 15582-15592.  
8994878 K.C.Holmes (1996).
Muscle proteins--their actions and interactions.
  Curr Opin Struct Biol, 6, 781-789.  
8805581 M.J.Cope, J.Whisstock, I.Rayment, and J.Kendrick-Jones (1996).
Conservation within the myosin motor domain: implications for structure and function.
  Structure, 4, 969-987.  
8981935 M.Sata, and M.Ikebe (1996).
Functional analysis of the mutations in the human cardiac beta-myosin that are responsible for familial hypertrophic cardiomyopathy. Implication for the clinical outcome.
  J Clin Invest, 98, 2866-2873.  
8896589 R.D.Vale (1996).
Switches, latches, and amplifiers: common themes of G proteins and molecular motors.
  J Cell Biol, 135, 291-302.  
8861898 S.M.Block (1996).
Fifty ways to love your lever: myosin motors.
  Cell, 87, 151-157.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.