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PDBsum entry 1v1o

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protein Protein-protein interface(s) links
Virulence factor PDB id
1v1o
Jmol
Contents
Protein chains
196 a.a. *
Waters ×36
* Residue conservation analysis
PDB id:
1v1o
Name: Virulence factor
Title: Staphylococcal superantigen-like protein 7
Structure: Exotoxin 1. Chain: a, b. Synonym: staphylococcal superantigen like protein 7. Engineered: yes
Source: Staphylococcus aureus. Organism_taxid: 1280. Strain: nctc6571. Expressed in: escherichia coli. Expression_system_taxid: 562. Expression_system_variant: jm109(prep4).
Biol. unit: Dimer (from PDB file)
Resolution:
2.75Å     R-factor:   0.213     R-free:   0.259
Authors: C.E.Naylor,D.C.Briggs,S.P.Nair,A.M.Al-Shangiti
Key ref: A.M.Al-Shangiti et al. (2004). Structural relationships and cellular tropism of staphylococcal superantigen-like proteins. Infect Immun, 72, 4261-4270. PubMed id: 15213171
Date:
21-Apr-04     Release date:   01-Jul-04    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
Q9ZFS5  (Q9ZFS5_STAAU) -  Exotoxin 1
Seq:
Struc:
231 a.a.
196 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     extracellular region   1 term 
  Biological process     pathogenesis   1 term 

 

 
Infect Immun 72:4261-4270 (2004)
PubMed id: 15213171  
 
 
Structural relationships and cellular tropism of staphylococcal superantigen-like proteins.
A.M.Al-Shangiti, C.E.Naylor, S.P.Nair, D.C.Briggs, B.Henderson, B.M.Chain.
 
  ABSTRACT  
 
The staphylococcal superantigen-like proteins (SSLs) are a family of polymorphic paralogs encoded in the Staphylococcus aureus genome whose function is unknown. The crystal structure of SSL7 was determined and compared to that of SSL5 and that of a classical superantigen, streptococcal pyrogenic exotoxin. Although the overall architecture of the superantigen family is retained in both SSL7 and SSL5, there are significant differences in the structures which suggest that the characteristic major histocompatibility complex binding site of superantigens has been lost. To complement these data, the abilities of SSL7 and a closely related paralog, SSL9, to interact with cells of the immune system were investigated. In populations of human white blood cells, both SSLs interacted selectively with monocytes via specific saturable but separate binding sites, which led to rapid uptake of the SSLs. In addition, SSLs were rapidly taken up by dendritic cells, but not by macrophages, into the same endosomal compartment as dextran. The ability of these secreted proteins to target antigen-presenting cells may enhance a misplaced antibody response against the proteins, which may facilitate bacterial colonization rather than contribute to host protection. Like classical superantigens, therefore, SSLs may distract the host's immune system, but they may do so via entirely different molecular mechanisms.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
20133685 N.S.Laursen, N.Gordon, S.Hermans, N.Lorenz, N.Jackson, B.Wines, E.Spillner, J.B.Christensen, M.Jensen, F.Fredslund, M.Bjerre, L.Sottrup-Jensen, J.D.Fraser, and G.R.Andersen (2010).
Structural basis for inhibition of complement C5 by the SSL7 protein from Staphylococcus aureus.
  Proc Natl Acad Sci U S A, 107, 3681-3686.
PDB codes: 3kls 3km9
20479083 S.Itoh, E.Hamada, G.Kamoshida, K.Takeshita, T.Oku, and T.Tsuji (2010).
Staphylococcal superantigen-like protein 5 inhibits matrix metalloproteinase 9 from human neutrophils.
  Infect Immun, 78, 3298-3305.  
18197169 J.D.Lambris, D.Ricklin, and B.V.Geisbrecht (2008).
Complement evasion by human pathogens.
  Nat Rev Microbiol, 6, 132-142.  
18507678 S.Monecke, P.Slickers, and R.Ehricht (2008).
Assignment of Staphylococcus aureus isolates to clonal complexes based on microarray analysis and pattern recognition.
  FEMS Immunol Med Microbiol, 53, 237-251.  
18039711 A.Zemla, B.Geisbrecht, J.Smith, M.Lam, B.Kirkpatrick, M.Wagner, T.Slezak, and C.E.Zhou (2007).
STRALCP--structure alignment-based clustering of proteins.
  Nucleic Acids Res, 35, e150.  
17848512 P.A.Ramsland, N.Willoughby, H.M.Trist, W.Farrugia, P.M.Hogarth, J.D.Fraser, and B.D.Wines (2007).
Structural basis for evasion of IgA immunity by Staphylococcus aureus revealed in the complex of SSL7 with Fc of human IgA1.
  Proc Natl Acad Sci U S A, 104, 15051-15056.
PDB code: 2qej
17873401 P.J.Haas, and J.van Strijp (2007).
Anaphylatoxins: their role in bacterial infection and inflammation.
  Immunol Res, 37, 161-175.  
16782791 P.J.Kundrotas, and E.Alexov (2006).
Electrostatic properties of protein-protein complexes.
  Biophys J, 91, 1724-1736.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.