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PDBsum entry 1v0n

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protein ligands links
Hydrolase PDB id
1v0n
Jmol
Contents
Protein chain
302 a.a. *
Ligands
EDO ×2
XIF-XYP
IMD
Waters ×618
* Residue conservation analysis
PDB id:
1v0n
Name: Hydrolase
Title: Xylanase xyn10a from streptomyces lividans in complex with xylobio-isofagomine at ph 7.5
Structure: Endo-1,4-beta-xylanase a. Chain: a. Fragment: catalytic module, residues 42-354. Synonym: xylanase a, 1,4-beta-d-xylan xylanohydrolase a. Engineered: yes
Source: Streptomyces lividans. Organism_taxid: 1916. Expressed in: streptomyces lividans. Expression_system_taxid: 1916.
Resolution:
1.10Å     R-factor:   0.104     R-free:   0.124
Authors: T.M.Gloster,S.J.Williams,S.Roberts,C.A.Tarling,J.Wicki, S.G.Withers,G.J.Davies
Key ref: T.M.Gloster et al. (2004). Atomic resolution analyses of the binding of xylobiose-derived deoxynojirimycin and isofagomine to xylanase Xyn10A. Chem Commun (Camb), 16, 1794-1795. PubMed id: 15306887 DOI: 10.1039/b405152a
Date:
31-Mar-04     Release date:   16-Aug-04    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P26514  (XYNA_STRLI) -  Endo-1,4-beta-xylanase A
Seq:
Struc:
477 a.a.
302 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: E.C.3.2.1.8  - Endo-1,4-beta-xylanase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Endohydrolysis of 1,4-beta-D-xylosidic linkages in xylans.
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     carbohydrate metabolic process   1 term 
  Biochemical function     hydrolase activity, hydrolyzing O-glycosyl compounds     1 term  

 

 
DOI no: 10.1039/b405152a Chem Commun (Camb) 16:1794-1795 (2004)
PubMed id: 15306887  
 
 
Atomic resolution analyses of the binding of xylobiose-derived deoxynojirimycin and isofagomine to xylanase Xyn10A.
T.M.Gloster, S.J.Williams, S.Roberts, C.A.Tarling, J.Wicki, S.G.Withers, G.J.Davies.
 
  ABSTRACT  
 
The atomic resolution structures of xylobiose-derived isofagomine and xylobiose-derived deoxynojirimycin in complex with the xylanase Xyn10A from Streptomyces lividans reveal undistorted (4)C(1) chair conformed sugars and, in the case of the deoxynojirimycin analogue, suggest unusual pK(a) changes of the enzyme's catalytic machinery upon binding.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
19532990 M.Aguilar-Moncayo, T.M.Gloster, J.P.Turkenburg, M.I.García-Moreno, C.Ortiz Mellet, G.J.Davies, and J.M.García Fernández (2009).
Glycosidase inhibition by ring-modified castanospermine analogues: tackling enzyme selectivity by inhibitor tailoring.
  Org Biomol Chem, 7, 2738-2747.
PDB codes: 2wbg 2wc3 2wc4
18074341 A.D.Hill, and P.J.Reilly (2008).
A Gibbs free energy correlation for automated docking of carbohydrates.
  J Comput Chem, 29, 1131-1141.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.