PDBsum entry: 1v0m

Hydrolase

PDB-id: 1v0m

Contents

Description

Header details

Header records

References

PROCHECK

Protein chain

303 a.a. *

Ligands

XDN-XYP

IMD

Waters ×568

* Residue conservation analysis

Tools

Clefts Calculation

PDB id:

1v0m

Name:

Hydrolase

Title:

Xylanase xyn10a from streptomyces lividans in complex with xylobio-deoxynojirimycin at ph 7.5

Structure:

Endo-1,4-beta-xylanase a. Chain: a. Fragment: catalytic module, residues 42-354. Synonym: xylanase a, 1,4-beta-d-xylan xylanohydrolase a. Engineered: yes

Source:

Streptomyces lividans. Organism_taxid: 1916. Expressed in: streptomyces lividans. Expression_system_taxid: 1916.

UniProt:

P26514 (XYNA_STRLI)

Seq:

Struc:

Seq:

477 a.a.

Struc:

303 a.a.

Key:	PfamA domain
Secondary structure	CATH domain

Enzyme class:

E.C.3.2.1.8   [IntEnz]   [ExPASy]   [KEGG]   [BRENDA]

Reaction:

Endohydrolysis of 1,4-beta-D-xylosidic linkages in xylans.

Resolution:

1.07Å

R-factor:

0.099

R-free:

0.121

Authors:

T.M.Gloster,S.J.Williams,S.Roberts,C.A.Tarling,J.Wicki, S.G.Withers,G.J.Davies

Key ref:

T.M.Gloster et al. (2004). Atomic resolution analyses of the binding of xylobiose-derived deoxynojirimycin and isofagomine to xylanase Xyn10A.. Chem Commun (camb), 16, 1794-1795. [PubMed id: 15306887] [DOI: 10.1039/b405152a]

Date:

31-Mar-04

Release date:

16-Aug-04

Related entries:

1e0v xylanase 10a from sreptomyces lividans. Cellobiosyl-enzyme intermediate at 1.7 a
1e0w xylanase 10a from sreptomyces lividans. Native structure at 1.2 angstrom resolution
1e0x xylanase 10a from sreptomyces lividans. Xylobiosyl-enzyme intermediate at 1.65 a
1knl streptomyces lividans xylan binding domain cbm13
1knm streptomyces lividans xylan binding domain cbm13 in complexwith lactose
1mc9 strepromyces lividans xylan binding domain cbm13 in complexwith xylopentaose
1od8 xylanase xyn10a from streptomyces lividans in complex with xylobio-isofagomine lactam
1v0l xylanase xyn10a from streptomyces lividans in complex with xylobio-isofagomine at ph 5.8
1v0k xylanase xyn10a from streptomyces lividans in complex with xylobio-deoxynojirimycin at ph 5.8
1v0n xylanase xyn10a from streptomyces lividans in complex with xylobio-isofagomine at ph 7.5
... plus others (see Header records)

Quick_links

Procheck

Clefts

Surface

Key reference

DOI no: 10.1039/b405152a

Chem Commun (camb) 16:1794-1795 (2004)

PubMed id: 15306887

Atomic resolution analyses of the binding of xylobiose-derived deoxynojirimycin and isofagomine to xylanase Xyn10A.

T.M.Gloster, S.J.Williams, S.Roberts, C.A.Tarling, J.Wicki, S.G.Withers, G.J.Davies.

ABSTRACT

The atomic resolution structures of xylobiose-derived isofagomine and xylobiose-derived deoxynojirimycin in complex with the xylanase Xyn10A from Streptomyces lividans reveal undistorted (4)C(1) chair conformed sugars and, in the case of the deoxynojirimycin analogue, suggest unusual pK(a) changes of the enzyme's catalytic machinery upon binding.