PDBsum entry 1v0h

Oxidoreductase

PDB id: 1v0h

Contents

Protein chain

249 a.a. *

Ligands

HEM

SHA

Metals

_NA

Waters ×403

* Residue conservation analysis

PDB id:

1v0h

Name:

Oxidoreductase

Title:

Ascobate peroxidase from soybean cytosol in complex with salicylhydroxamic acid

Structure:

Ascorbate peroxidase. Chain: x. Engineered: yes. Other_details: salicylhydroxamic acid complex

Source:

Glycine max. Soybean. Organism_taxid: 3847. Expressed in: escherichia coli. Expression_system_taxid: 562. Other_details: n-terminal 6-his tag

Resolution:

1.46Å R-factor: 0.151 R-free: 0.182

Authors:

K.H.Sharp,E.L.Raven,P.C.E.Moody

Key ref:

K.H.Sharp et al. (2004). Crystal structure of the ascorbate peroxidase-salicylhydroxamic acid complex. Biochemistry, 43, 8644-8651. PubMed id: 15236572 DOI: 10.1021/bi049343q

Date:

29-Mar-04 Release date: 23-Jun-04

Protein chain

Q43758  (Q43758_SOYBN) -  L-ascorbate peroxidase from Glycine max

Seq: 250 a.a.

Struc: 249 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.1.11.1.11 - L-ascorbate peroxidase.
• Reaction: L-ascorbate + H2O2 = L-dehydroascorbate + 2 H2O

L-ascorbate + H2O2 (Bound ligand (Het Group name = SHA) matches with 53.33% similarity) = L-dehydroascorbate + 2 × H2O

• Cofactor: Heme

Heme (Bound ligand (Het Group name = HEM) matches with 95.45% similarity)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

DOI no: 10.1021/bi049343q

Biochemistry 43:8644-8651 (2004)

PubMed id: 15236572

Crystal structure of the ascorbate peroxidase-salicylhydroxamic acid complex.

K.H.Sharp, P.C.Moody, K.A.Brown, E.L.Raven.

ABSTRACT

Ascorbate peroxidase is a bifunctional peroxidase that catalyzes the H(2)O(2)-dependent oxidation of both ascorbate and various aromatic substrates. The ascorbate binding site was recently identified as being close to the gamma-heme edge [Sharp, K. H., Mewies, M., Moody, P. C. E., and Raven, E. L. (2003)Nat. Struct. Biol. 10, 303-307]. In this work, the X-ray crystal structure of recombinant soybean cytosolic ascorbate peroxidase (rsAPX) in complex with salicylhydroxamic acid (SHA) has been determined to 1.46 A. The SHA molecule is bound close to the delta-heme edge in a cavity that connects the distal side of the heme to the surface of the protein. There are hydrogen bonds between the phenolic hydroxide of the SHA and the main chain carbonyl of Pro132, between the carbonyl oxygen of SHA and the side chain guanadinium group of Arg38, and between the hydroxamic acid group and the indole nitrogen of Trp41. The structure provides the first information about the location of the aromatic binding site in ascorbate peroxidase and, together with our previous data [Sharp, K. H., et al. (2003) Nat. Struct. Biol. 10, 303-307], completes the structural description of the binding properties of ascorbate peroxidase. The mechanistic implications of the results are discussed in terms of our current understanding of how APX catalyzes oxidation of different types of substrates bound at different locations.