spacer
spacer

PDBsum entry 1tvm

Go to PDB code: 
protein links
Transferase PDB id
1tvm
Jmol
Contents
Protein chain
113 a.a. *
* Residue conservation analysis
PDB id:
1tvm
Name: Transferase
Title: Nmr structure of enzyme gatb of the galactitol-specific phosphoenolpyruvate-dependent phosphotransferase system
Structure: Pts system, galactitol-specific iib component. Chain: a. Synonym: eiib-gat, galacticol-permease iib component, phosphotransferase enzyme ii, b component, gatb. Engineered: yes
Source: Escherichia coli. Organism_taxid: 562. Gene: gatb, c2618, z3256, ecs2896, sf2155, s2281. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
NMR struc: 20 models
Authors: L.Volpon,C.R.Young,N.S.Lim,P.Iannuzzi,M.Cygler,K.Gehring, Montreal-Kingston Bacterial Structural Genomics Initiative (Bsgi)
Key ref: L.Volpon et al. (2006). NMR structure of the enzyme GatB of the galactitol-specific phosphoenolpyruvate-dependent phosphotransferase system and its interaction with GatA. Protein Sci, 15, 2435-2441. PubMed id: 16963640 DOI: 10.1110/ps.062337406
Date:
29-Jun-04     Release date:   06-Sep-05    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P0A435  (PTKB_ECOL6) -  Galactitol-specific phosphotransferase enzyme IIB component
Seq:
Struc:
94 a.a.
113 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     cytoplasm   1 term 
  Biological process     transport   5 terms 
  Biochemical function     transferase activity     2 terms  

 

 
DOI no: 10.1110/ps.062337406 Protein Sci 15:2435-2441 (2006)
PubMed id: 16963640  
 
 
NMR structure of the enzyme GatB of the galactitol-specific phosphoenolpyruvate-dependent phosphotransferase system and its interaction with GatA.
L.Volpon, C.R.Young, A.Matte, K.Gehring.
 
  ABSTRACT  
 
The phosphoenolpyruvate-dependent carbohydrate transport system (PTS) couples uptake with phosphorylation of a variety of carbohydrates in prokaryotes. In this multienzyme complex, the enzyme II (EII), a carbohydrate-specific permease, is constituted of two cytoplasmic domains, IIA and IIB, and a transmembrane channel IIC domain. Among the five families of EIIs identified in Escherichia coli, the galactitol-specific transporter (II(gat)) belongs to the glucitol family and is structurally the least well-characterized. Here, we used nuclear magnetic resonance (NMR) spectroscopy to solve the three-dimensional structure of the IIB subunit (GatB). GatB consists of a central four-stranded parallel beta-sheet flanked by alpha-helices on both sides; the active site cysteine of GatB is located at the beginning of an unstructured loop between beta1 and alpha1 that folds into a P-loop-like structure. This structural arrangement shows similarities with other IIB subunits but also with mammalian low molecular weight protein tyrosine phosphatases (LMW PTPase) and arsenate reductase (ArsC). An NMR titration was performed to identify the GatA-interacting residues.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
17766377 E.León, A.Yee, A.R.Ortíz, J.Santoro, M.Rico, and M.A.Jiménez (2007).
Solution structure of the hypothetical protein TA0095 from Thermoplasma acidophilum: a novel superfamily with a two-layer sandwich architecture.
  Protein Sci, 16, 2278-2286.
PDB code: 2joi
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.