PDBsum entry: 1tsp

Late protein

PDB-id

1tsp


	Asymmetric unit

Contents

Description

Header details

Protein chain

Waters ×218

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		Biological unit, trimer (as deduced by PQS)

PDB id:

1tsp

Name:

Late protein

Title:

Crystal structure of p22 tailspike protein: interdigitated subunits in a thermostable trimer

Structure:

Tailspike-protein. Chain: a. Synonym: tsp

Source:

Enterobacteria phage p22. Organism_taxid: 10754

Biological unit:

Trimer (from PQS)

UniProt:

P12528 (TSPE_BPP22)

Seq:

Struc:

Seq:

Struc:

Seq:

667 a.a.

Struc:

544 a.a.*

Key:		PfamA domain
		Secondary structure			CATH domain

* PDB and UniProt seqs differ at 1 residue position (black cross)

Resolution:

2.00Å

R-factor:

0.184

Authors:

S.Steinbacher,R.Seckler,S.Miller,B.Steipe,R.Huber,P.Reinemer

Key ref:

S.Steinbacher et al. (1994). Crystal structure of P22 tailspike protein: interdigitated subunits in a thermostable trimer.. Science, 265, 383-386. [PubMed id: 8023158] [DOI: 10.1126/science.8023158]

Date:

16-Jun-94

Release date:

15-Sep-95

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Key reference

DOI no: 10.1126/science.8023158 Science 265:383-386 (1994)

PubMed id: 8023158

Crystal structure of P22 tailspike protein: interdigitated subunits in a thermostable trimer.

S.Steinbacher, R.Seckler, S.Miller, B.Steipe, R.Huber, P.Reinemer.

ABSTRACT

The tailspike protein (TSP) of Salmonella typhimurium phage P22 is a part of the apparatus by which the phage attaches to the bacterial host and hydrolyzes the O antigen. It has served as a model system for genetic and biochemical analysis of protein folding. The x-ray structure of a shortened TSP (residues 109 to 666) was determined to a 2.0 angstrom resolution. Each subunit of the homotrimer contains a large parallel beta helix. The interdigitation of the polypeptide chains at the carboxyl termini is important to protrimer formation in the folding pathway and to thermostability of the mature protein.

Literature references that cite this PDB file's key reference

PubMed id Reference

20118935 E.C.Schulz, A.Dickmanns, H.Urlaub, A.Schmitt, M.Mühlenhoff, K.Stummeyer, D.Schwarzer, R.Gerardy-Schahn, and R.Ficner (2010).
Crystal structure of an intramolecular chaperone mediating triple-beta-helix folding.

Nat Struct Mol Biol, 17, 210-215.

18826406 A.S.Olia, and G.Cingolani (2008).
A shifty stop for a hairy tail.

Mol Microbiol, 70, 549-553.

18077713 M.Walter, C.Fiedler, R.Grassl, M.Biebl, R.Rachel, X.L.Hermo-Parrado, A.L.Llamas-Saiz, R.Seckler, S.Miller, and M.J.van Raaij (2008).
Structure of the receptor-binding protein of bacteriophage det7: a podoviral tail spike in a myovirus.

J Virol, 82, 2265-2273.

PDB code: 2v5i

19091116 R.Villafane, M.Zayas, E.B.Gilcrease, A.M.Kropinski, and S.R.Casjens (2008).
Genomic analysis of bacteriophage epsilon34 of Salmonella enterica serovar Anatum (15+).

BMC Microbiol, 8, 227.

18547389 S.Barbirz, J.J.Müller, C.Uetrecht, A.J.Clark, U.Heinemann, and R.Seckler (2008).
Crystal structure of Escherichia coli phage HK620 tailspike: podoviral tailspike endoglycosidase modules are evolutionarily related.

Mol Microbiol, 69, 303-316.

PDB codes: 2vji 2vjj

17611601 C.Plisson, H.E.White, I.Auzat, A.Zafarani, C.São-José, S.Lhuillier, P.Tavares, and E.V.Orlova (2007).
Structure of bacteriophage SPP1 tail reveals trigger for DNA ejection.

EMBO J, 26, 3720-3728.

17655498 R.Mishra, R.Bhat, and R.Seckler (2007).
Chemical chaperone-mediated protein folding: stabilization of P22 tailspike folding intermediates by glycerol.

Biol Chem, 388, 797-804.

16547930 A.V.McDonnell, M.Menke, N.Palmer, J.King, L.Cowen, and B.Berger (2006).
Fold recognition and accurate sequence-structure alignment of sequences directing beta-sheet proteins.

Proteins, 63, 976-985.

16417644 D.T.Pride, T.M.Wassenaar, C.Ghose, and M.J.Blaser (2006).
Evidence of host-virus co-evolution in tetranucleotide usage patterns of bacteriophages and eukaryotic viruses.

BMC Genomics, 7, 8.

16751612 J.Kim, and A.S.Robinson (2006).
Dissociation of intermolecular disulfide bonds in P22 tailspike protein intermediates in the presence of SDS.

Protein Sci, 15, 1791-1793.

16689790 K.Stummeyer, D.Schwarzer, H.Claus, U.Vogel, R.Gerardy-Schahn, and M.Mühlenhoff (2006).
Evolution of bacteriophages infecting encapsulated bacteria: lessons from Escherichia coli K1-specific phages.

Mol Microbiol, 60, 1123-1135.

16555353 K.Sugimoto, S.Kanamaru, K.Iwasaki, F.Arisaka, and I.Yamashita (2006).
Construction of a ball-and-spike protein supramolecule.

Angew Chem Int Ed Engl, 45, 2725-2728.

16505375 R.Simkovsky, and J.King (2006).
An elongated spine of buried core residues necessary for in vivo folding of the parallel beta-helix of P22 tailspike adhesin.

Proc Natl Acad Sci U S A, 103, 3575-3580.

17053784 Y.Xiang, M.C.Morais, A.J.Battisti, S.Grimes, P.J.Jardine, D.L.Anderson, and M.G.Rossmann (2006).
Structural changes of bacteriophage phi29 upon DNA packaging and release.

EMBO J, 25, 5229-5239.

15608653 K.Stummeyer, A.Dickmanns, M.Mühlenhoff, R.Gerardy-Schahn, and R.Ficner (2005).
Crystal structure of the polysialic acid-degrading endosialidase of bacteriophage K1F.

Nat Struct Mol Biol, 12, 90-96.

PDB codes: 1v0e 1v0f

16081648 M.J.Gage, J.L.Zak, and A.S.Robinson (2005).
Three amino acids that are critical to formation and stability of the P22 tailspike trimer.

Protein Sci, 14, 2333-2343.

16179922 M.S.Evans, K.G.Ugrinov, M.A.Frese, and P.L.Clark (2005).
Homogeneous stalled ribosome nascent chain complexes produced in vivo or in vitro.

Nat Methods, 2, 757-762.

16314578 N.L.Smith, E.J.Taylor, A.M.Lindsay, S.J.Charnock, J.P.Turkenburg, E.J.Dodson, G.J.Davies, and G.W.Black (2005).
Structure of a group A streptococcal phage-encoded virulence factor reveals a catalytically active triple-stranded beta-helix.

Proc Natl Acad Sci U S A, 102, 17652-17657.

PDB code: 2c3f

16302960 O.S.Makin, and L.C.Serpell (2005).
Structures for amyloid fibrils.

FEBS J, 272, 5950-5961.

16096708 R.Villafane, S.Costa, R.Ahmed, and C.Salgado (2005).
Conservation of the N-terminus of some phage tail proteins.

Arch Virol, 150, 2609-2621.

15143215 A.V.Kajava, U.Baxa, R.B.Wickner, and A.C.Steven (2004).
A model for Ure2p prion filaments and other amyloids: the parallel superpleated beta-structure.

Proc Natl Acad Sci U S A, 101, 7885-7890.

15133163 B.G.Lefebvre, N.K.Comolli, M.J.Gage, and A.S.Robinson (2004).
Pressure dissociation studies provide insight into oligomerization competence of temperature-sensitive folding mutants of P22 tailspike.

Protein Sci, 13, 1538-1546.

15356868 P.McPhie (2004).
CD studies on films of amyloid proteins and polypeptides: quantitative g-factor analysis indicates a common folding motif.

Biopolymers, 75, 140-147.

15322277 S.Betts, C.Haase-Pettingell, K.Cook, and J.King (2004).
Buried hydrophobic side-chains essential for the folding of the parallel beta-helix domains of the P22 tailspike.

Protein Sci, 13, 2291-2303.

15294822 W.H.Pope, C.Haase-Pettingell, and J.King (2004).
Protein folding failure sets high-temperature limit on growth of phage P22 in Salmonella enterica serovar Typhimurium.

Appl Environ Microbiol, 70, 4840-4847.

12652483 B.G.Lefebvre, and A.S.Robinson (2003).
Pressure treatment of tailspike aggregates rapidly produces on-pathway folding intermediates.

Biotechnol Bioeng, 82, 595-604.

14581223 B.L.Danek, and A.S.Robinson (2003).
Nonnative interactions between cysteines direct productive assembly of P22 tailspike protein.

Biophys J, 85, 3237-3247.

14627734 M.J.Gage, and A.S.Robinson (2003).
C-terminal hydrophobic interactions play a critical role in oligomeric assembly of the P22 tailspike trimer.

Protein Sci, 12, 2732-2747.

12837775 P.R.Weigele, E.Scanlon, and J.King (2003).
Homotrimeric, beta-stranded viral adhesins and tail proteins.

J Bacteriol, 185, 4022-4030.

12417558 A.Horwich (2002).
Protein aggregation in disease: a role for folding intermediates forming specific multimeric interactions.

J Clin Invest, 110, 1221-1232.

12012434 G.J.Thomas (2002).
New structural insights from Raman spectroscopy of proteins and their assemblies.

Biopolymers, 67, 214-225.

11910025 J.F.Kreisberg, S.D.Betts, C.Haase-Pettingell, and J.King (2002).
The interdigitated beta-helix domain of the P22 tailspike protein acts as a molecular clamp in trimer stabilization.

Protein Sci, 11, 820-830.

12169615 J.G.Lawrence, G.F.Hatfull, and R.W.Hendrix (2002).
Imbroglios of viral taxonomy: genetic exchange and failings of phenetic approaches.

J Bacteriol, 184, 4891-4905.

12015881 L.Cowen, P.Bradley, M.Menke, J.King, and B.Berger (2002).
Predicting the beta-helix fold from protein sequence data.

J Comput Biol, 9, 261-276.

11703654 A.V.Kajava, N.Cheng, R.Cleaver, M.Kessel, M.N.Simon, E.Willery, F.Jacob-Dubuisson, C.Locht, and A.C.Steven (2001).
Beta-helix model for the filamentous haemagglutinin adhesin of Bordetella pertussis and related bacterial secretory proteins.

Mol Microbiol, 42, 279-292.

11266625 C.Haase-Pettingell, S.Betts, S.W.Raso, L.Stuart, A.Robinson, and J.King (2001).
Role for cysteine residues in the in vivo folding and assembly of the phage P22 tailspike.

Protein Sci, 10, 397-410.

11717490 M.Akita, A.Suzuki, T.Kobayashi, S.Ito, and T.Yamane (2001).
The first structure of pectate lyase belonging to polysaccharide lyase family 3.

Acta Crystallogr D Biol Crystallogr, 57, 1786-1792.

PDB code: 1ee6

11530935 M.J.van Raaij, G.Schoehn, M.Jaquinod, K.Ashman, M.R.Burda, and S.Miller (2001).
Identification and crystallisation of a heat- and protease-stable fragment of the bacteriophage T4 short tail fibre.

Biol Chem, 382, 1049-1055.

10737931 B.Schuler, F.Fürst, F.Osterroth, S.Steinbacher, R.Huber, and R.Seckler (2000).
Plasticity and steric strain in a parallel beta-helix: rational mutations in the P22 tailspike protein.

Proteins, 39, 89.

PDB codes: 1qq1 1qrb 1qrc

11123920 D.E.Kamen, Y.Griko, and R.W.Woody (2000).
The stability, structural organization, and denaturation of pectate lyase C, a parallel beta-helix protein.

Biochemistry, 39, 15932-15943.

11206055 J.F.Kreisberg, S.D.Betts, and J.King (2000).
Beta-helix core packing within the triple-stranded oligomerization domain of the P22 tailspike.

Protein Sci, 9, 2338-2343.

10653812 R.Khurana, and A.L.Fink (2000).
Do parallel beta-helix proteins have a unique fourier transform infrared spectrum?

Biophys J, 78, 994.

10922032 S.R.Herron, J.A.Benen, R.D.Scavetta, J.Visser, and F.Jurnak (2000).
Structure and function of pectic enzymes: virulence factors of plant pathogens.

Proc Natl Acad Sci U S A, 97, 8762-8769.

10491109 A.Mitraki, A.Barge, J.Chroboczek, J.P.Andrieu, J.Gagnon, and R.W.Ruigrok (1999).
Unfolding studies of human adenovirus type 2 fibre trimers. Evidence for a stable domain.

Eur J Biochem, 264, 599-606.

10397811 D.Foguel, C.R.Robinson, P.C.de Sousa, J.L.Silva, and A.S.Robinson (1999).
Hydrostatic pressure rescues native protein from aggregates.

Biotechnol Bioeng, 63, 552-558.

10022824 J.L.Jiménez, J.I.Guijarro, E.Orlova, J.Zurdo, C.M.Dobson, M.Sunde, and H.R.Saibil (1999).
Cryo-electron microscopy structure of an SH3 amyloid fibril and model of the molecular packing.

EMBO J, 18, 815-821.

10504409 M.R.Burda, and S.Miller (1999).
Folding of coliphage T4 short tail fiber in vitro. Analysing the role of a bacteriophage-encoded chaperone.

Eur J Biochem, 265, 771-778.

10408337 N.D.Lazo, and D.T.Downing (1999).
Fibril formation by amyloid-beta proteins may involve beta-helical protofibrils.

J Pept Res, 53, 633-640.

10606266 P.S.Page-McCaw, K.Amonlirdviman, and P.A.Sharp (1999).
PUF60: a novel U2AF65-related splicing activity.

RNA, 5, 1548-1560.

9672040 C.S.Wang, T.Lozano-Pérez, and B.Tidor (1998).
AmbiPack: a systematic algorithm for packing of macromolecular structures with ambiguous distance constraints.

Proteins, 32, 26-42.

9712782 M.McClelland, and R.K.Wilson (1998).
Comparison of sample sequences of the Salmonella typhi genome to the sequence of the complete Escherichia coli K-12 genome.

Infect Immun, 66, 4305-4312.

9492738 N.D.Lazo, and D.T.Downing (1998).
Amyloid fibrils may be assembled from beta-helical protofibrils.

Biochemistry, 37, 1731-1735.

9684883 S.D.Betts, and J.King (1998).
Cold rescue of the thermolabile tailspike intermediate at the junction between productive folding and off-pathway aggregation.

Protein Sci, 7, 1516-1523.

9636063 S.Miller, B.Schuler, and R.Seckler (1998).
A reversibly unfolding fragment of P22 tailspike protein with native structure: the isolated beta-helix domain.

Biochemistry, 37, 9160-9168.

9792111 S.Miller, B.Schuler, and R.Seckler (1998).
Phage P22 tailspike protein: removal of head-binding domain unmasks effects of folding mutations on native-state thermal stability.

Protein Sci, 7, 2223-2232.

9187652 A.S.Robinson, and J.King (1997).
Disulphide-bonded intermediate on the folding and assembly pathway of a non-disulphide bonded protein.

Nat Struct Biol, 4, 450-455.

15012275 K.E.Hammond-Kosack, and J.D.Jones (1997).
PLANT DISEASE RESISTANCE GENES.

Annu Rev Plant Physiol Plant Mol Biol, 48, 575-607.

18634100 M.A.Speed, J.King, and D.I.Wang (1997).
Polymerization mechanism of polypeptide chain aggregation.

Biotechnol Bioeng, 54, 333-343.

8946845 B.Kobe (1996).
Leucines on a roll.

Nat Struct Biol, 3, 977-980.

8665839 C.Kisker, H.Schindelin, B.E.Alber, J.G.Ferry, and D.C.Rees (1996).
A left-hand beta-helix revealed by the crystal structure of a carbonic anhydrase from the archaeon Methanosarcina thermophila.

EMBO J, 15, 2323-2330.

PDB code: 1thj

9631094 M.A.Speed, D.I.Wang, and J.King (1996).
Specific aggregation of partially folded polypeptide chains: the molecular basis of inclusion body composition.

Nat Biotechnol, 14, 1283-1287.

8639573 S.D.Betts, J.R.Ross, E.Pichersky, and C.F.Yocum (1996).
Cold-sensitive assembly of a mutant manganese-stabilizing protein caused by a Val to Ala replacement.

Biochemistry, 35, 6302-6307.

8855221 S.Steinbacher, U.Baxa, S.Miller, A.Weintraub, R.Seckler, and R.Huber (1996).
Crystal structure of phage P22 tailspike protein complexed with Salmonella sp. O-antigen receptors.

Proc Natl Acad Sci U S A, 93, 10584-10588.

PDB codes: 1tyu 1tyv 1tyw 1tyx

8889178 U.Baxa, S.Steinbacher, S.Miller, A.Weintraub, R.Huber, and R.Seckler (1996).
Interactions of phage P22 tails with their cellular receptor, Salmonella O-antigen polysaccharide.

Biophys J, 71, 2040-2048.

7663345 M.A.Speed, D.I.Wang, and J.King (1995).
Multimeric intermediates in the pathway to the aggregated inclusion body state for P22 tailspike polypeptide chains.

Protein Sci, 4, 900-908.

7583673 P.T.Lansbury, P.R.Costa, J.M.Griffiths, E.J.Simon, M.Auger, K.J.Halverson, D.A.Kocisko, Z.S.Hendsch, T.T.Ashburn, and R.G.Spencer (1995).
Structural model for the beta-amyloid fibril based on interstrand alignment of an antiparallel-sheet comprising a C-terminal peptide.

Nat Struct Biol, 2, 990-998.

8539248 V.Sieber, F.Jurnak, and G.R.Moe (1995).
Circular dichroism of the parallel beta helical proteins pectate lyase C and E.

Proteins, 23, 32-37.

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.