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PDBsum entry 1ts5

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protein Protein-protein interface(s) links
Toxin PDB id
1ts5
Jmol
Contents
Protein chains
194 a.a. *
Waters ×20
* Residue conservation analysis
PDB id:
1ts5
Name: Toxin
Title: I140t mutant of toxic shock syndrome toxin-1 from s. Aureus
Structure: Toxic shock syndrome toxin-1. Chain: a, b. Synonym: tsst-1. Engineered: yes. Mutation: yes
Source: Staphylococcus aureus. Organism_taxid: 1280. Expressed in: staphylococcus aureus. Expression_system_taxid: 1280
Biol. unit: Monomer (from PDB file)
Resolution:
3.10Å     R-factor:   0.179    
Authors: C.A.Earhart,D.T.Mitchell,D.L.Murray,D.M.Pinheiro, M.Matsumura,P.M.Schlievert,D.H.Ohlendorf
Key ref:
C.A.Earhart et al. (1998). Structures of five mutants of toxic shock syndrome toxin-1 with reduced biological activity. Biochemistry, 37, 7194-7202. PubMed id: 9585531 DOI: 10.1021/bi9721896
Date:
10-Oct-97     Release date:   16-Dec-98    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
P06886  (TSST_STAAU) -  Toxic shock syndrome toxin-1
Seq:
Struc:
234 a.a.
194 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 2 residue positions (black crosses)

 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     extracellular region   1 term 
  Biological process     pathogenesis   1 term 

 

 
DOI no: 10.1021/bi9721896 Biochemistry 37:7194-7202 (1998)
PubMed id: 9585531  
 
 
Structures of five mutants of toxic shock syndrome toxin-1 with reduced biological activity.
C.A.Earhart, D.T.Mitchell, D.L.Murray, D.M.Pinheiro, M.Matsumura, P.M.Schlievert, D.H.Ohlendorf.
 
  ABSTRACT  
 
The three-dimensional structures of five mutants of toxic shock syndrome toxin-1 (TSST-1) have been determined. These mutations are in the long central alpha helix and are useful in mapping portions of TSST-1 involved in superantigenicity and lethality. The T128A, H135A, Q139K, and I140T mutations appear to reduce superantigenicity by altering the properties of the T-cell receptor interaction surface. The Q136A mutation is at a largely buried site and causes a dramatic change in the conformation of the beta7-beta9 loop which covers the back of the central alpha helix. As this mutation has the unique ability to reduce the toxin's lethality in rabbits while retaining its superantigenicity, it raises the possibility that this rear loop mediates the ability of TSST-1 to induce lethality and suggests a route for producing nonlethal toxins for therapeutic development.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
19012411 A.J.Brosnahan, M.M.Schaefers, W.H.Amundson, M.J.Mantz, C.A.Squier, M.L.Peterson, and P.M.Schlievert (2008).
Novel toxic shock syndrome toxin-1 amino acids required for biological activity.
  Biochemistry, 47, 12995-13003.  
18419534 D.Devore-Carter, S.Kar, V.Vellucci, V.Bhattacherjee, P.Domanski, and M.K.Hostetter (2008).
Superantigen-like effects of a Candida albicans polypeptide.
  J Infect Dis, 197, 981-989.  
18687870 Y.Kuwahara, A.Ohno, T.Morii, H.Yokoyama, I.Matsui, H.Tochio, M.Shirakawa, and H.Hiroaki (2008).
The solution structure of the C-terminal domain of NfeD reveals a novel membrane-anchored OB-fold.
  Protein Sci, 17, 1915-1924.
PDB code: 2exd
18039711 A.Zemla, B.Geisbrecht, J.Smith, M.Lam, B.Kirkpatrick, M.Wagner, T.Slezak, and C.E.Zhou (2007).
STRALCP--structure alignment-based clustering of proteins.
  Nucleic Acids Res, 35, e150.  
17131300 A.Bavoso, A.Ostuni, J.De Vendel, F.Pollaro, F.Armentano, T.Knight, S.Makker, and A.Tramontano (2006).
Aldehyde modification of peptide immunogen enhances protein-reactive antibody response to toxic shock syndrome toxin-1.
  J Pept Sci, 12, 843-849.  
15691839 B.V.Geisbrecht, B.Y.Hamaoka, B.Perman, A.Zemla, and D.J.Leahy (2005).
The crystal structures of EAP domains from Staphylococcus aureus reveal an unexpected homology to bacterial superantigens.
  J Biol Chem, 280, 17243-17250.
PDB codes: 1yn3 1yn4 1yn5
11544350 J.K.McCormick, J.M.Yarwood, and P.M.Schlievert (2001).
Toxic shock syndrome and bacterial superantigens: an update.
  Annu Rev Microbiol, 55, 77.  
10627489 M.M.Dinges, P.M.Orwin, and P.M.Schlievert (2000).
Exotoxins of Staphylococcus aureus.
  Clin Microbiol Rev, 13, 16.  
10721486 W.W.Kum, K.B.Laupland, and A.W.Chow (2000).
Defining a novel domain of staphylococcal toxic shock syndrome toxin-1 critical for major histocompatibility complex class II binding, superantigenic activity, and lethality.
  Can J Microbiol, 46, 171-179.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.