PDBsum entry: 1tq1

Structural genomics, unknown function

PDB-id: 1tq1

Contents

Description

Header details

Header records

References

PROCHECK

Protein chain

119 a.a. *

* Residue conservation analysis

Tools

Clefts Calculation

PDB id:

1tq1

Name:

Structural genomics, unknown function

Title:

Solution structure of at5g66040, a putative protein from arabidosis thaliana

Structure:

Senescence-associated family protein. Chain: a. Synonym: at5g66040. Engineered: yes

Source:

Arabidopsis thaliana. Thale cress. Organism_taxid: 3702. Expressed in: cell-free synthesis. Other_details: wheat germ cell-free, in vitro expression

UniProt:

Q39129 (STR16_ARATH)

Seq:

120 a.a.

Struc:

119 a.a.*

Key:	PfamA domain
Secondary structure	CATH domain

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme class:

E.C.2.8.1.1   [IntEnz]   [ExPASy]   [KEGG]   [BRENDA]

Reaction:

Thiosulfate + cyanide = sulfite + thiocyanate (see diagram below)

Resolution:

not givenÅ

NMR structure:

5 models

Authors:

C.C.Cornilescu,G.Cornilescu,S.Singh,M.S.Lee,E.M.Tyler, M.N.Shahan,D.Vinarov,J.L.Markley,Center For Eukaryotic Structural Genomics (Cesg)

Key ref:

G.Cornilescu et al. (2006). Solution structure of a single-domain thiosulfate sulfurtransferase from Arabidopsis thaliana.. Protein Sci, 15, 2836-2841. [PubMed id: 17088324] [DOI: 10.1110/ps.062395206]

Date:

16-Jun-04

Release date:

29-Jun-04

Related entries:

Go.20862 related db: targetdb

Quick_links

Procheck

Clefts

Surface

Key reference

DOI no: 10.1110/ps.062395206

Protein Sci 15:2836-2841 (2006)

PubMed id: 17088324

Solution structure of a single-domain thiosulfate sulfurtransferase from Arabidopsis thaliana.

G.Cornilescu, D.A.Vinarov, E.M.Tyler, J.L.Markley, C.C.Cornilescu.

ABSTRACT

We describe the three-dimensional structure of the product of Arabidopsis thaliana gene At5g66040.1 as determined by NMR spectroscopy. This protein is categorized as single-domain sulfurtransferase and is annotated as a senescence-associated protein (sen1-like protein) and ketoconazole resistance protein (http://arabidopsis.org/info/genefamily/STR_genefamily.html). The sequence of At5g66040.1 is virtually identical to that of a protein from Arabidopsis found by others to confer ketoconazole resistance in yeast. Comparison of the three-dimensional structure with those in the Protein Data Bank revealed that At5g66040.1 contains an additional mobile beta-hairpin not found in other rhodaneses that may function in binding specific substrates. This represents the first structure of a single-domain plant sulfurtransferase. The enzymatically active cysteine-containing domain belongs to the CDC25 class of phosphatases, sulfide dehydrogenases, and stress proteins such as senescence specific protein 1 in plants, PspE and GlpE in bacteria, and cyanide and arsenate resistance proteins. Versions of this domain that lack the active site cysteine are found in other proteins, such as phosphatases, ubiquitin hydrolases, and sulfuryltransferases.

Selected figure(s)

Figure 3.
Figure 3. (A) Comparison of ribbon diagrams for At5g66040.1; GlpE, an Str from E. coli (PDB ID 1GMX); a polysulfide-sulfur

The above figure is reprinted by permission from the Protein Society: Protein Sci (2006, 15, 2836-2841) copyright 2006.

Figure was selected by the author.