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Binding protein(gtp)
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PDB id
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1tnd
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* Residue conservation analysis
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Gene Ontology (GO) functional annotation
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Cellular component
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photoreceptor inner segment
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6 terms
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Biological process
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response to stimulus
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18 terms
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Biochemical function
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acyl binding
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10 terms
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DOI no:
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Nature
366:654-663
(1993)
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PubMed id:
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The 2.2 A crystal structure of transducin-alpha complexed with GTP gamma S.
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J.P.Noel,
H.E.Hamm,
P.B.Sigler.
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ABSTRACT
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The 2.2 A crystal structure of activated rod transducin, Gt alpha.GTP gamma S,
shows the bound GTP gamma S molecule occluded deep in a cleft between a domain
structurally homologous to small GTPases and a helical domain unique to
heterotrimeric G proteins. The structure, when combined with biochemical and
genetic studies, suggests: how an activated receptor might open this cleft to
allow nucleotide exchange; a mechanism for GTP-induced changes in effector and
receptor binding surfaces; and a mechanism for GTPase activity not evident from
previous data.
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Literature references that cite this PDB file's key reference
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| |
PubMed id
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Reference
|
|
|
|
|
|
G.J.Kang,
Z.J.Gong,
J.A.Cheng,
Z.R.Zhu,
and
C.G.Mao
(2011).
Cloning and expression analysis of a G-protein α subunit--Gαo in the rice water weevil Lissorhoptrus oryzophilus Kuschel.
|
| |
Arch Insect Biochem Physiol, 76,
43-54.
|
|
|
|
|
|
|
B.R.Temple,
C.D.Jones,
and
A.M.Jones
(2010).
Evolution of a signaling nexus constrained by protein interfaces and conformational States.
|
| |
PLoS Comput Biol, 6,
e1000962.
|
|
|
|
|
|
|
F.D.Ivey,
F.X.Taglia,
F.Yang,
M.M.Lander,
D.A.Kelly,
and
C.S.Hoffman
(2010).
Activated alleles of the Schizosaccharomyces pombe gpa2+ Galpha gene identify residues involved in GDP-GTP exchange.
|
| |
Eukaryot Cell, 9,
626-633.
|
|
|
|
|
|
|
L.Shen,
G.Caruso,
P.Bisegna,
D.Andreucci,
V.V.Gurevich,
H.E.Hamm,
and
E.Dibenedetto
(2010).
Dynamics of mouse rod phototransduction and its sensitivity to variation of key parameters.
|
| |
IET Syst Biol, 4,
12.
|
|
|
|
|
|
|
N.A.Lambert,
C.A.Johnston,
S.D.Cappell,
S.Kuravi,
A.J.Kimple,
F.S.Willard,
and
D.P.Siderovski
(2010).
Regulators of G-protein signaling accelerate GPCR signaling kinetics and govern sensitivity solely by accelerating GTPase activity.
|
| |
Proc Natl Acad Sci U S A, 107,
7066-7071.
|
|
|
|
|
|
|
A.M.Preininger,
M.A.Funk,
W.M.Oldham,
S.M.Meier,
C.A.Johnston,
S.Adhikary,
A.J.Kimple,
D.P.Siderovski,
H.E.Hamm,
and
T.M.Iverson
(2009).
Helix dipole movement and conformational variability contribute to allosteric GDP release in Galphai subunits.
|
| |
Biochemistry, 48,
2630-2642.
|
|
|
PDB code:
|
|
|
|
|
|
|
|
B.Jastrzebska,
M.Golczak,
D.Fotiadis,
A.Engel,
and
K.Palczewski
(2009).
Isolation and functional characterization of a stable complex between photoactivated rhodopsin and the G protein, transducin.
|
| |
FASEB J, 23,
371-381.
|
|
|
|
|
|
|
H.E.Hamm,
S.M.Meier,
G.Liao,
and
A.M.Preininger
(2009).
Trp fluorescence reveals an activation-dependent cation-pi interaction in the Switch II region of Galphai proteins.
|
| |
Protein Sci, 18,
2326-2335.
|
|
|
|
|
|
|
K.Khafizov
(2009).
GoLoco motif proteins binding to Galpha(i1): insights from molecular simulations.
|
| |
J Mol Model, 15,
1491-1499.
|
|
|
|
|
|
|
T.D.Bunney,
O.Opaleye,
S.M.Roe,
P.Vatter,
R.W.Baxendale,
C.Walliser,
K.L.Everett,
M.B.Josephs,
C.Christow,
F.Rodrigues-Lima,
P.Gierschik,
L.H.Pearl,
and
M.Katan
(2009).
Structural insights into formation of an active signaling complex between Rac and phospholipase C gamma 2.
|
| |
Mol Cell, 34,
223-233.
|
|
|
PDB codes:
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|
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|
|
W.Baehr,
and
J.M.Frederick
(2009).
Naturally occurring animal models with outer retina phenotypes.
|
| |
Vision Res, 49,
2636-2652.
|
|
|
|
|
|
|
W.E.McIntire
(2009).
Structural determinants involved in the formation and activation of G protein betagamma dimers.
|
| |
Neurosignals, 17,
82-99.
|
|
|
|
|
|
|
Y.L.Wong,
K.A.Dietrich,
N.Naber,
R.Cooke,
and
S.E.Rice
(2009).
The Kinesin-1 tail conformationally restricts the nucleotide pocket.
|
| |
Biophys J, 96,
2799-2807.
|
|
|
|
|
|
|
A.Goc,
T.E.Angel,
B.Jastrzebska,
B.Wang,
P.L.Wintrode,
and
K.Palczewski
(2008).
Different properties of the native and reconstituted heterotrimeric G protein transducin.
|
| |
Biochemistry, 47,
12409-12419.
|
|
|
|
|
|
|
A.M.Preininger,
J.Parello,
S.M.Meier,
G.Liao,
and
H.E.Hamm
(2008).
Receptor-mediated changes at the myristoylated amino terminus of Galpha(il) proteins.
|
| |
Biochemistry, 47,
10281-10293.
|
|
|
|
|
|
|
A.R.Zurita,
and
L.Birnbaumer
(2008).
The same mutation in Gsalpha and transducin alpha reveals behavioral differences between these highly homologous G protein alpha-subunits.
|
| |
Proc Natl Acad Sci U S A, 105,
2363-2368.
|
|
|
|
|
|
|
A.V.Smrcka,
D.M.Lehmann,
and
A.L.Dessal
(2008).
G protein betagamma subunits as targets for small molecule therapeutic development.
|
| |
Comb Chem High Throughput Screen, 11,
382-395.
|
|
|
|
|
|
|
E.McCusker,
and
A.S.Robinson
(2008).
Refolding of G protein alpha subunits from inclusion bodies expressed in Escherichia coli.
|
| |
Protein Expr Purif, 58,
342-355.
|
|
|
|
|
|
|
K.C.Slep,
M.A.Kercher,
T.Wieland,
C.K.Chen,
M.I.Simon,
and
P.B.Sigler
(2008).
Molecular architecture of Galphao and the structural basis for RGS16-mediated deactivation.
|
| |
Proc Natl Acad Sci U S A, 105,
6243-6248.
|
|
|
PDB codes:
|
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|
|
|
|
|
|
K.Sayar,
O.UÄŸur,
T.Liu,
V.J.Hilser,
and
O.Onaran
(2008).
Exploring allosteric coupling in the alpha-subunit of Heterotrimeric G proteins using evolutionary and ensemble-based approaches.
|
| |
BMC Struct Biol, 8,
23.
|
|
|
|
|
|
|
M.J.Phillips,
G.Calero,
B.Chan,
S.Ramachandran,
and
R.A.Cerione
(2008).
Effector proteins exert an important influence on the signaling-active state of the small GTPase Cdc42.
|
| |
J Biol Chem, 283,
14153-14164.
|
|
|
PDB code:
|
|
|
|
|
|
|
|
V.Hauryliuk,
S.Hansson,
and
M.Ehrenberg
(2008).
Cofactor dependent conformational switching of GTPases.
|
| |
Biophys J, 95,
1704-1715.
|
|
|
|
|
|
|
W.M.Oldham,
and
H.E.Hamm
(2008).
Heterotrimeric G protein activation by G-protein-coupled receptors.
|
| |
Nat Rev Mol Cell Biol, 9,
60-71.
|
|
|
|
|
|
|
Z.Chen,
W.D.Singer,
S.M.Danesh,
P.C.Sternweis,
and
S.R.Sprang
(2008).
Recognition of the activated states of Galpha13 by the rgRGS domain of PDZRhoGEF.
|
| |
Structure, 16,
1532-1543.
|
|
|
PDB codes:
|
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|
|
|
B.Barren,
and
N.O.Artemyev
(2007).
Mechanisms of dominant negative G-protein alpha subunits.
|
| |
J Neurosci Res, 85,
3505-3514.
|
|
|
|
|
|
|
F.Mancia,
and
W.A.Hendrickson
(2007).
Expression of recombinant G-protein coupled receptors for structural biology.
|
| |
Mol Biosyst, 3,
723-734.
|
|
|
|
|
|
|
K.Montgomery,
E.Suzuki,
M.Kessler,
and
A.C.Arai
(2007).
Factors affecting guanine nucleotide binding to rat AMPA receptors.
|
| |
Brain Res, 1177,
1-8.
|
|
|
|
|
|
|
L.S.Weinstein,
T.Xie,
Q.H.Zhang,
and
M.Chen
(2007).
Studies of the regulation and function of the Gs alpha gene Gnas using gene targeting technology.
|
| |
Pharmacol Ther, 115,
271-291.
|
|
|
|
|
|
|
L.Vogeley,
V.D.Trivedi,
O.A.Sineshchekov,
E.N.Spudich,
J.L.Spudich,
and
H.Luecke
(2007).
Crystal structure of the Anabaena sensory rhodopsin transducer.
|
| |
J Mol Biol, 367,
741-751.
|
|
|
PDB codes:
|
|
|
|
|
|
|
|
M.Jaffé,
and
J.Bubis
(2007).
Use of 5'-[p-(fluorosulfonyl)benzoyl] guanosine as an affinity probe for the guanine nucleotide-binding site of transducin.
|
| |
Protein J, 26,
125-133.
|
|
|
|
|
|
|
P.S.Crozier,
M.J.Stevens,
and
T.B.Woolf
(2007).
How a small change in retinal leads to G-protein activation: initial events suggested by molecular dynamics calculations.
|
| |
Proteins, 66,
559-574.
|
|
|
|
|
|
|
W.M.Oldham,
N.Van Eps,
A.M.Preininger,
W.L.Hubbell,
and
H.E.Hamm
(2007).
Mapping allosteric connections from the receptor to the nucleotide-binding pocket of heterotrimeric G proteins.
|
| |
Proc Natl Acad Sci U S A, 104,
7927-7932.
|
|
|
|
|
|
|
Y.Wu,
T.Buranda,
P.C.Simons,
G.P.Lopez,
W.E.McIntire,
J.C.Garrison,
E.R.Prossnitz,
and
L.A.Sklar
(2007).
Rapid-mix flow cytometry measurements of subsecond regulation of G protein-coupled receptor ternary complex dynamics by guanine nucleotides.
|
| |
Anal Biochem, 371,
10-20.
|
|
|
|
|
|
|
C.Galés,
J.J.Van Durm,
S.Schaak,
S.Pontier,
Y.Percherancier,
M.Audet,
H.Paris,
and
M.Bouvier
(2006).
Probing the activation-promoted structural rearrangements in preassembled receptor-G protein complexes.
|
| |
Nat Struct Mol Biol, 13,
778-786.
|
|
|
|
|
|
|
C.Hill,
A.Goddard,
J.Davey,
and
G.Ladds
(2006).
Investigating RGS proteins in yeast.
|
| |
Semin Cell Dev Biol, 17,
352-362.
|
|
|
|
|
|
|
C.L.Piscitelli,
T.E.Angel,
B.W.Bailey,
P.Hargrave,
E.A.Dratz,
and
C.M.Lawrence
(2006).
Equilibrium between metarhodopsin-I and metarhodopsin-II is dependent on the conformation of the third cytoplasmic loop.
|
| |
J Biol Chem, 281,
6813-6825.
|
|
|
PDB code:
|
|
|
|
|
|
|
|
G.Milligan,
and
E.Kostenis
(2006).
Heterotrimeric G-proteins: a short history.
|
| |
Br J Pharmacol, 147,
S46-S55.
|
|
|
|
|
|
|
J.E.Grant,
L.W.Guo,
M.M.Vestling,
K.A.Martemyanov,
V.Y.Arshavsky,
and
A.E.Ruoho
(2006).
The N terminus of GTP gamma S-activated transducin alpha-subunit interacts with the C terminus of the cGMP phosphodiesterase gamma-subunit.
|
| |
J Biol Chem, 281,
6194-6202.
|
|
|
|
|
|
|
K.D.Ridge,
N.G.Abdulaev,
C.Zhang,
T.Ngo,
D.M.Brabazon,
and
J.P.Marino
(2006).
Conformational changes associated with receptor-stimulated guanine nucleotide exchange in a heterotrimeric G-protein alpha-subunit: NMR analysis of GTPgammaS-bound states.
|
| |
J Biol Chem, 281,
7635-7648.
|
|
|
|
|
|
|
M.A.Anderson,
B.Ogbay,
O.G.Kisselev,
D.P.Cistola,
and
G.R.Marshall
(2006).
Alternate binding mode of C-terminal phenethylamine analogs of G(t)alpha(340-350) to photoactivated rhodopsin.
|
| |
Chem Biol Drug Des, 68,
295-307.
|
|
|
|
|
|
|
N.Van Eps,
W.M.Oldham,
H.E.Hamm,
and
W.L.Hubbell
(2006).
Structural and dynamical changes in an alpha-subunit of a heterotrimeric G protein along the activation pathway.
|
| |
Proc Natl Acad Sci U S A, 103,
16194-16199.
|
|
|
|
|
|
|
S.Majumdar,
S.Ramachandran,
and
R.A.Cerione
(2006).
New insights into the role of conserved, essential residues in the GTP binding/GTP hydrolytic cycle of large G proteins.
|
| |
J Biol Chem, 281,
9219-9226.
|
|
|
|
|
|
|
S.Ramachandran,
and
R.A.Cerione
(2006).
How GPCRs hit the switch.
|
| |
Nat Struct Mol Biol, 13,
756-757.
|
|
|
|
|
|
|
V.V.Gurevich,
and
E.V.Gurevich
(2006).
The structural basis of arrestin-mediated regulation of G-protein-coupled receptors.
|
| |
Pharmacol Ther, 110,
465-502.
|
|
|
|
|
|
|
C.R.McCudden,
M.D.Hains,
R.J.Kimple,
D.P.Siderovski,
and
F.S.Willard
(2005).
G-protein signaling: back to the future.
|
| |
Cell Mol Life Sci, 62,
551-577.
|
|
|
|
|
|
|
L.L.Anderson,
G.R.Marshall,
E.Crocker,
S.O.Smith,
and
T.J.Baranski
(2005).
Motion of carboxyl terminus of Galpha is restricted upon G protein activation. A solution NMR study using semisynthetic Galpha subunits.
|
| |
J Biol Chem, 280,
31019-31026.
|
|
|
|
|
|
|
M.E.Burns,
and
V.Y.Arshavsky
(2005).
Beyond counting photons: trials and trends in vertebrate visual transduction.
|
| |
Neuron, 48,
387-401.
|
|
|
|
|
|
|
N.G.Abdulaev,
C.Zhang,
A.Dinh,
T.Ngo,
P.N.Bryan,
D.M.Brabazon,
J.P.Marino,
and
K.D.Ridge
(2005).
Bacterial expression and one-step purification of an isotope-labeled heterotrimeric G-protein alpha-subunit.
|
| |
J Biomol NMR, 32,
31-40.
|
|
|
|
|
|
|
N.G.Abdulaev,
T.Ngo,
C.Zhang,
A.Dinh,
D.M.Brabazon,
K.D.Ridge,
and
J.P.Marino
(2005).
Heterotrimeric G-protein alpha-subunit adopts a "preactivated" conformation when associated with betagamma-subunits.
|
| |
J Biol Chem, 280,
38071-38080.
|
|
|
|
|
|
|
R.Mishra,
S.K.Gara,
S.Mishra,
and
B.Prakash
(2005).
Analysis of GTPases carrying hydrophobic amino acid substitutions in lieu of the catalytic glutamine: implications for GTP hydrolysis.
|
| |
Proteins, 59,
332-338.
|
|
|
|
|
|
|
R.Pereira,
and
R.A.Cerione
(2005).
A switch 3 point mutation in the alpha subunit of transducin yields a unique dominant-negative inhibitor.
|
| |
J Biol Chem, 280,
35696-35703.
|
|
|
|
|
|
|
S.Albrizio,
G.Caliendo,
G.D'Errico,
E.Novellino,
P.Rovero,
and
A.M.D'Ursi
(2005).
Galphas protein C-terminal alpha-helix at the interface: does the plasma membrane play a critical role in the Galphas protein functionality?
|
| |
J Pept Sci, 11,
617-626.
|
|
|
|
|
|
|
T.F.Reubold,
S.Eschenburg,
A.Becker,
M.Leonard,
S.L.Schmid,
R.B.Vallee,
F.J.Kull,
and
D.J.Manstein
(2005).
Crystal structure of the GTPase domain of rat dynamin 1.
|
| |
Proc Natl Acad Sci U S A, 102,
13093-13098.
|
|
|
PDB code:
|
|
|
|
|
|
|
|
A.H.Hawasli,
O.Saifee,
C.Liu,
M.L.Nonet,
and
C.M.Crowder
(2004).
Resistance to volatile anesthetics by mutations enhancing excitatory neurotransmitter release in Caenorhabditis elegans.
|
| |
Genetics, 168,
831-843.
|
|
|
|
|
|
|
A.Y.Wu,
X.B.Tang,
S.E.Martinez,
K.Ikeda,
and
J.A.Beavo
(2004).
Molecular determinants for cyclic nucleotide binding to the regulatory domains of phosphodiesterase 2A.
|
| |
J Biol Chem, 279,
37928-37938.
|
|
|
|
|
|
|
B.Ahvazi,
K.M.Boeshans,
and
P.M.Steinert
(2004).
Crystal structure of transglutaminase 3 in complex with GMP: structural basis for nucleotide specificity.
|
| |
J Biol Chem, 279,
26716-26725.
|
|
|
PDB code:
|
|
|
|
|
|
|
|
B.Ahvazi,
K.M.Boeshans,
W.Idler,
U.Baxa,
P.M.Steinert,
and
F.Rastinejad
(2004).
Structural basis for the coordinated regulation of transglutaminase 3 by guanine nucleotides and calcium/magnesium.
|
| |
J Biol Chem, 279,
7180-7192.
|
|
|
PDB codes:
|
|
|
|
|
|
|
|
C.J.Thomas,
X.Du,
P.Li,
Y.Wang,
E.M.Ross,
and
S.R.Sprang
(2004).
Uncoupling conformational change from GTP hydrolysis in a heterotrimeric G protein alpha-subunit.
|
| |
Proc Natl Acad Sci U S A, 101,
7560-7565.
|
|
|
PDB codes:
|
|
|
|
|
|
|
|
S.Majumdar,
S.Ramachandran,
and
R.A.Cerione
(2004).
Perturbing the linker regions of the alpha-subunit of transducin: a new class of constitutively active GTP-binding proteins.
|
| |
J Biol Chem, 279,
40137-40145.
|
|
|
|
|
|
|
V.Radhika,
D.Onesime,
J.H.Ha,
and
N.Dhanasekaran
(2004).
Galpha13 stimulates cell migration through cortactin-interacting protein Hax-1.
|
| |
J Biol Chem, 279,
49406-49413.
|
|
|
|
|
|
|
J.Cherfils,
and
M.Chabre
(2003).
Activation of G-protein Galpha subunits by receptors through Galpha-Gbeta and Galpha-Ggamma interactions.
|
| |
Trends Biochem Sci, 28,
13-17.
|
|
|
|
|
|
|
J.L.Gu,
W.Lu,
C.Xia,
X.Wu,
and
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