PDBsum entry: 1tfm

Toxin

PDB id: 1tfm

Contents

Protein chains

240 a.a. *

255 a.a. *

Ligands

NAG

P6C

NAG-NAG

NAG-NAG-BMA-BMA

NAG-NAG-BMA

BGC-GAL

GAL

Waters ×106

* Residue conservation analysis

PDB id:

1tfm

Name:

Toxin

Title:

Crystal structure of a ribosome inactivating protein in its inhibited form

Structure:

Himalayan mistletoe ribosome-inactivating protein chain: a. Himalayan mistletoe ribosome-inactivating protein chain: b

Source:

Viscum album. European mistletoe. Organism_taxid: 3972. Organism_taxid: 3972

Biol. unit:

Tetramer (from PQS)

Resolution:

2.80Å R-factor: 0.240 R-free: 0.289

Authors:

V.Mishra,S.Bilgrami,M.Paramasivam,S.Yadav,R.S.Sharma,P.Kaur, A.Srinivasan,C.R.Babu,T.P.Singh

Key ref:

V.Mishra et al. Crystal structure of a ribosome inactivating protein in its naturally inhibited form. To be published,

Date:

27-May-04 Release date: 13-Jul-04

Protein chain

Q6ITZ3  (ML4_VISAL) -  Beta-galactoside-specific lectin 4

Seq: 520 a.a.

Struc: 240 a.a.

Protein chain

Q6ITZ3  (ML4_VISAL) -  Beta-galactoside-specific lectin 4

Seq: 520 a.a.

Struc: 255 a.a.

Enzyme reactions

• Enzyme class: Chains A, B: E.C.3.2.2.22 - rRNA N-glycosylase.
• Reaction: Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.

 Gene Ontology (GO) functional annotation 

• Biological process: negative regulation of translation (1 term)
• Biochemical function: rRNA N-glycosylase activity (1 term)