PDBsum entry: 1sz6

Hydrolase inhibitor

PDB id: 1sz6

Contents

Protein chains

249 a.a. *

263 a.a. *

Ligands

NAG ×4

SO4

AZI ×3

GOL ×15

Metals

_CL ×2

Waters ×318

* Residue conservation analysis

PDB id:

1sz6

Name:

Hydrolase inhibitor

Title:

Mistletoe lectin i from viscum album. Crystal structure at 2 resolution

Structure:

Rrna n-glycosidase a chain. Chain: a. Beta-galactoside specific lectin i b chain. Chain: b

Source:

Viscum album. European mistletoe. Organism_taxid: 3972. Organism_taxid: 3972

Biol. unit:

Dimer (from PDB file)

Resolution:

2.05Å R-factor: 0.211 R-free: 0.253

Authors:

A.G.Gabdoulkhakov,N.E.Guhlistova,A.V.Lyashenko,R.Krauspenhaa S.Stoeva,W.Voelter,S.V.Nikonov,C.Betzel,A.M.Mikhailov

Key ref:

A.G.Gabdoulkhakov et al. Crystal structure of viscum album mistletoe lectin i in native state at 2.05 a resolution, Comparison of structure active site conformation in ricin and in viscumin. To be published,

Date:

04-Apr-04 Release date: 05-Oct-04

Protein chain

P81446  (ML1_VISAL) -  Beta-galactoside-specific lectin 1

Seq: 564 a.a.

Struc: 249 a.a.*

Protein chain

P81446  (ML1_VISAL) -  Beta-galactoside-specific lectin 1

Seq: 564 a.a.

Struc: 263 a.a.*

* PDB and UniProt seqs differ at 41 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chains A, B: E.C.3.2.2.22 - rRNA N-glycosylase.
• Reaction: Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.

 Gene Ontology (GO) functional annotation 

• Biological process: negative regulation of translation (1 term)
• Biochemical function: rRNA N-glycosylase activity (1 term)