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PDBsum entry 1slc
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protein ligands Protein-protein interface(s) links
Complex(lectin/saccharide) PDB id
1slc

 

 

 

 

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Contents
Protein chains
134 a.a. *
Ligands
NAG-BMA-MAN-NAG-
GAL-MAN-NAG-GAL
NDG-BMA-MAN-NAG-
GAL-MAN-NAG-GAL
Waters ×442
* Residue conservation analysis
PDB id:
1slc
Name: Complex(lectin/saccharide)
Title: X-ray crystallography reveals crosslinking of mammalian lectin (galectin-1) by biantennary complex type saccharides
Structure: Bovine galectin-1. Chain: a, b, c, d. Engineered: yes
Source: Bos taurus. Cow. Organism_taxid: 9913. Tissue: heart
Resolution:
2.15Å     R-factor:   0.194    
Authors: Y.Bourne,C.Cambillau
Key ref: Y.Bourne et al. (1994). Crosslinking of mammalian lectin (galectin-1) by complex biantennary saccharides. Nat Struct Biol, 1, 863-870. PubMed id: 7773775
Date:
12-Mar-94     Release date:   26-Jan-95    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
P11116  (LEG1_BOVIN) -  Galectin-1 from Bos taurus
Seq:
Struc: 		135 a.a.
134 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 

 
Nat Struct Biol 1:863-870 (1994)
PubMed id: 7773775  
 
 
Crosslinking of mammalian lectin (galectin-1) by complex biantennary saccharides.
Y.Bourne, B.Bolgiano, D.I.Liao, G.Strecker, P.Cantau, O.Herzberg, T.Feizi, C.Cambillau.
 
  ABSTRACT  
 
Galectins are beta-galactoside-binding proteins that occur intra- and extracellularly in many animal tissues. They have been proposed to form networks of glycoconjugates on the cell surface, where they may modulate various cell response pathways such as growth, activation and adhesion. The high resolution X-ray crystallographic analyses of three crystal forms of bovine galectin-1 in complex with biantennary saccharides of N-acetyllactosamine type reveal infinite chains of lectin dimers cross-linked through N-acetyllactosamine units located at the end of the oligosaccharide antenna. The oligosaccharide adopts a different low energy conformation in each of the three crystal forms.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
20813129 D.Zhang, S.Jiang, Y.Hu, S.Cui, H.Guo, K.Wu, Y.Li, and T.Su (2011).
A multidomain galectin involved in innate immune response of pearl oyster Pinctada fucata.
  Dev Comp Immunol, 35, 1-6.  
20864568 L.A.Earl, S.Bi, and L.G.Baum (2011).
Galectin multimerization and lattice formation are regulated by linker region structure.
  Glycobiology, 21, 6.  
19938027 E.Wéber, A.Hetényi, B.Váczi, E.Szolnoki, R.Fajka-Boja, V.Tubak, E.Monostori, and T.A.Martinek (2010).
Galectin-1-asialofetuin interaction is inhibited by peptides containing the tyr-xxx-tyr motif acting on the glycoprotein.
  Chembiochem, 11, 228-234.  
20146714 F.T.Liu, and G.A.Rabinovich (2010).
Galectins: regulators of acute and chronic inflammation.
  Ann N Y Acad Sci, 1183, 158-182.  
20004209 P.J.Coombs, R.Harrison, S.Pemberton, A.Quintero-Martinez, S.Parry, S.M.Haslam, A.Dell, M.E.Taylor, and K.Drickamer (2010).
Identification of novel contributions to high-affinity glycoprotein-receptor interactions using engineered ligands.
  J Mol Biol, 396, 685-696.  
20466650 T.Schallus, K.Fehér, U.Sternberg, V.Rybin, and C.Muhle-Goll (2010).
Analysis of the specific interactions between the lectin domain of malectin and diglucosides.
  Glycobiology, 20, 1010-1020.
PDB code: 2kr2
20006954 C.Meynier, M.Feracci, M.Espeli, F.Chaspoul, P.Gallice, C.Schiff, F.Guerlesquin, and P.Roche (2009).
NMR and MD investigations of human galectin-1/oligosaccharide complexes.
  Biophys J, 97, 3168-3177.  
19594632 D.K.Hsu, H.Y.Chen, and F.T.Liu (2009).
Galectin-3 regulates T-cell functions.
  Immunol Rev, 230, 114-127.  
19541770 M.C.Miller, A.Klyosov, and K.H.Mayo (2009).
The alpha-galactomannan Davanat binds galectin-1 at a site different from the conventional galectin carbohydrate binding domain.
  Glycobiology, 19, 1034-1045.  
19432560 M.C.Miller, I.V.Nesmelova, D.Platt, A.Klyosov, and K.H.Mayo (2009).
The carbohydrate-binding domain on galectin-1 is more extensive for a complex glycan than for simple saccharides: implications for galectin-glycan interactions at the cell surface.
  Biochem J, 421, 211-221.  
19103599 S.R.Stowell, M.Cho, C.L.Feasley, C.M.Arthur, X.Song, J.K.Colucci, S.Karmakar, P.Mehta, M.Dias-Baruffi, R.P.McEver, and R.D.Cummings (2009).
Ligand reduces galectin-1 sensitivity to oxidative inactivation by enhancing dimer formation.
  J Biol Chem, 284, 4989-4999.  
18393823 P.K.Qasba, B.Ramakrishnan, and E.Boeggeman (2008).
Structure and function of beta -1,4-galactosyltransferase.
  Curr Drug Targets, 9, 292-309.  
18456665 S.R.Stowell, C.M.Arthur, K.A.Slanina, J.R.Horton, D.F.Smith, and R.D.Cummings (2008).
Dimeric Galectin-8 induces phosphatidylserine exposure in leukocytes through polylactosamine recognition by the C-terminal domain.
  J Biol Chem, 283, 20547-20559.  
17961826 J.M.Belitsky, A.Nelson, J.D.Hernandez, L.G.Baum, and J.F.Stoddart (2007).
Multivalent interactions between lectins and supramolecular complexes: Galectin-1 and self-assembled pseudopolyrotaxanes.
  Chem Biol, 14, 1140-1151.  
16391767 J.M.Belitsky, A.Nelson, and J.F.Stoddart (2006).
Monitoring cyclodextrin-polyviologen pseudopolyrotaxanes with the Bradford assay.
  Org Biomol Chem, 4, 250-256.  
16584127 P.K.Qasba, B.Ramakrishnan, and E.Boeggeman (2006).
Mutant glycosyltransferases assist in the development of a targeted drug delivery system and contrast agents for MRI.
  AAPS J, 8, E190-E195.  
16972013 T.Shirai, C.Shionyu-Mitsuyama, T.Ogawa, and K.Muramoto (2006).
Structure based studies of the adaptive diversification process of congerins.
  Mol Divers, 10, 567-573.  
15465324 G.R.Vasta, H.Ahmed, and E.W.Odom (2004).
Structural and functional diversity of lectin repertoires in invertebrates, protochordates and ectothermic vertebrates.
  Curr Opin Struct Biol, 14, 617-630.  
14672941 N.Ahmad, H.J.Gabius, S.André, H.Kaltner, S.Sabesan, R.Roy, B.Liu, F.Macaluso, and C.F.Brewer (2004).
Galectin-3 precipitates as a pentamer with synthetic multivalent carbohydrates and forms heterogeneous cross-linked complexes.
  J Biol Chem, 279, 10841-10847.  
12517452 J.D.Schrag, D.O.Procopio, M.Cygler, D.Y.Thomas, and J.J.Bergeron (2003).
Lectin control of protein folding and sorting in the secretory pathway.
  Trends Biochem Sci, 28, 49-57.  
12829506 L.He, S.André, H.C.Siebert, H.Helmholz, B.Niemeyer, and H.J.Gabius (2003).
Detection of ligand- and solvent-induced shape alterations of cell-growth-regulatory human lectin galectin-1 in solution by small angle neutron and x-ray scattering.
  Biophys J, 85, 511-524.  
14517974 M.G.Ford, T.Weimar, T.Köhli, and R.J.Woods (2003).
Molecular dynamics simulations of galectin-1-oligosaccharide complexes reveal the molecular basis for ligand diversity.
  Proteins, 53, 229-240.  
11809773 K.E.Pace, T.Lebestky, T.Hummel, P.Arnoux, K.Kwan, and L.G.Baum (2002).
Characterization of a novel Drosophila melanogaster galectin. Expression in developing immune, neural, and muscle tissues.
  J Biol Chem, 277, 13091-13098.  
11846886 K.Scott, and J.Zhang (2002).
Partial identification by site-directed mutagenesis of a cell growth inhibitory site on the human galectin-1 molecule.
  BMC Cell Biol, 3, 3.  
12271131 L.Gauthier, B.Rossi, F.Roux, E.Termine, and C.Schiff (2002).
Galectin-1 is a stromal cell ligand of the pre-B cell receptor (BCR) implicated in synapse formation between pre-B and stromal cells and in pre-BCR triggering.
  Proc Natl Acad Sci U S A, 99, 13014-13019.  
11258914 J.C.Sacchettini, L.G.Baum, and C.F.Brewer (2001).
Multivalent protein-carbohydrate interactions. A new paradigm for supermolecular assembly and signal transduction.
  Biochemistry, 40, 3009-3015.  
10880979 G.M.Bradbrook, J.R.Forshaw, and S.Pérez (2000).
Structure/thermodynamics relationships of lectin-saccharide complexes: the Erythrina corallodendron case.
  Eur J Biochem, 267, 4545-4555.  
10410803 C.A.Bush, M.Martin-Pastor, and A.Imberty (1999).
Structure and conformation of complex carbohydrates of glycoproteins, glycolipids, and bacterial polysaccharides.
  Annu Rev Biophys Biomol Struct, 28, 269-293.  
10545323 T.Shirai, C.Mitsuyama, Y.Niwa, Y.Matsui, H.Hotta, T.Yamane, H.Kamiya, C.Ishii, T.Ogawa, and K.Muramoto (1999).
High-resolution structure of the conger eel galectin, congerin I, in lactose-liganded and ligand-free forms: emergence of a new structure class by accelerated evolution.
  Structure, 7, 1223-1233.
PDB codes: 1c1f 1c1l
9760227 D.D.Leonidas, E.H.Vatzaki, H.Vorum, J.E.Celis, P.Madsen, and K.R.Acharya (1998).
Structural basis for the recognition of carbohydrates by human galectin-7.
  Biochemistry, 37, 13930-13940.
PDB codes: 1bkz 2gal 3gal 4gal 5gal
9582341 J.Seetharaman, A.Kanigsberg, R.Slaaby, H.Leffler, S.H.Barondes, and J.M.Rini (1998).
X-ray crystal structure of the human galectin-3 carbohydrate recognition domain at 2.1-A resolution.
  J Biol Chem, 273, 13047-13052.
PDB code: 1a3k
9857034 W.Cheng, E.Bullitt, L.Bhattacharyya, C.F.Brewer, and L.Makowski (1998).
Electron microscopy and x-ray diffraction studies of Lotus tetragonolobus A isolectin cross-linked with a divalent Lewisx oligosaccharide, an oncofetal antigen.
  J Biol Chem, 273, 35016-35022.  
9345618 A.Imberty (1997).
Oligosaccharide structures: theory versus experiment.
  Curr Opin Struct Biol, 7, 617-623.  
  9234729 A.Vyakarnam, S.F.Dagher, J.L.Wang, and R.J.Patterson (1997).
Evidence for a role for galectin-1 in pre-mRNA splicing.
  Mol Cell Biol, 17, 4730-4737.  
8999822 D.N.Cooper, R.P.Boulianne, S.Charlton, E.M.Farrell, A.Sucher, and B.C.Lu (1997).
Fungal galectins, sequence and specificity of two isolectins from Coprinus cinereus.
  J Biol Chem, 272, 1514-1521.  
9363750 H.C.Siebert, R.Adar, R.Arango, M.Burchert, H.Kaltner, G.Kayser, E.Tajkhorshid, C.W.von der Lieth, R.Kaptein, N.Sharon, J.F.Vliegenthart, and H.J.Gabius (1997).
Involvement of laser photo-CIDNP (chemically induced dynamic nuclear polarization)-reactive amino acid side chains in ligand binding by galactoside-specific lectins in solution.
  Eur J Biochem, 249, 27-38.  
9057819 H.J.Gabius (1997).
Animal lectins.
  Eur J Biochem, 243, 543-576.  
9334250 Y.Arata, J.Hirabayashi, and K.Kasai (1997).
Structure of the 32-kDa galectin gene of the nematode Caenorhabditis elegans.
  J Biol Chem, 272, 26669-26677.  
  8844838 C.S.Wright, and G.E.Kellogg (1996).
Differences in hydropathic properties of ligand binding at four independent sites in wheat germ agglutinin-oligosaccharide crystal complexes.
  Protein Sci, 5, 1466-1476.  
8939757 C.S.Wright, and G.Hester (1996).
The 2.0 A structure of a cross-linked complex between snowdrop lectin and a branched mannopentaose: evidence for two unique binding modes.
  Structure, 4, 1339-1352.
PDB code: 1jpc
8955156 H.Ahmed, J.Pohl, N.E.Fink, F.Strobel, and G.R.Vasta (1996).
The primary structure and carbohydrate specificity of a beta-galactosyl-binding lectin from toad (Bufo arenarum Hensel) ovary reveal closer similarities to the mammalian galectin-1 than to the galectin from the clawed frog Xenopus laevis.
  J Biol Chem, 271, 33083-33094.  
8855944 M.Cho, and R.D.Cummings (1996).
Characterization of monomeric forms of galectin-1 generated by site-directed mutagenesis.
  Biochemistry, 35, 13081-13088.  
8704151 P.Booij, R.A.Demel, B.S.de Pater, and J.W.Kijne (1996).
Insertion of pea lectin into a phospholipid monolayer.
  Plant Mol Biol, 31, 169-173.  
8747464 D.D.Leonidas, B.L.Elbert, Z.Zhou, H.Leffler, S.J.Ackerman, and K.R.Acharya (1995).
Crystal structure of human Charcot-Leyden crystal protein, an eosinophil lysophospholipase, identifies it as a new member of the carbohydrate-binding family of galectins.
  Structure, 3, 1379-1393.
PDB code: 1lcl
8749360 D.I.Stuart, and E.Y.Jones (1995).
Recognition at the cell surface: recent structural insights.
  Curr Opin Struct Biol, 5, 735-743.  
8574697 J.M.Rini (1995).
X-ray crystal structures of animal lectins.
  Curr Opin Struct Biol, 5, 617-621.  
8574696 K.Drickamer (1995).
Increasing diversity of animal lectin structures.
  Curr Opin Struct Biol, 5, 612-616.  
8575449 M.Menéndez, M.Gasset, J.Laynez, C.López-Zumel, P.Usobiaga, E.Töpfer-Petersen, and J.J.Calvete (1995).
Analysis of the structural organization and thermal stability of two spermadhesins. Calorimetric, circular dichroic and Fourier-transform infrared spectroscopic studies.
  Eur J Biochem, 234, 887-896.  
7493989 P.Kuhn, C.Guan, T.Cui, A.L.Tarentino, T.H.Plummer, and P.Van Roey (1995).
Active site and oligosaccharide recognition residues of peptide-N4-(N-acetyl-beta-D-glucosaminyl)asparagine amidase F.
  J Biol Chem, 270, 29493-29497.
PDB code: 1pnf
8574693 R.J.Woods (1995).
Three-dimensional structures of oligosaccharides.
  Curr Opin Struct Biol, 5, 591-598.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.

 

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