PDBsum entry: 1sgy

Hydrolase/hydrolase inhibitor

PDB id: 1sgy

Contents

Protein chains

185 a.a. *

51 a.a. *

Ligands

PO4

Waters ×151

* Residue conservation analysis

PDB id:

1sgy

Name:

Hydrolase/hydrolase inhibitor

Title:

Tyr 18 variant of turkey ovomucoid inhibitor third domain complexed with streptomyces griseus proteinase b at ph 6.5

Structure:

Streptogrisin b. Chain: e. Synonym: protease b, sgpb, pronase enzyme b. Ovomucoid. Chain: i. Fragment: third domain. Synonym: tyr18-omtky3. Engineered: yes. Mutation: yes

Source:

Streptomyces griseus. Organism_taxid: 1911. Strain: k1. Meleagris gallopavo. Turkey. Organism_taxid: 9103. Expressed in: escherichia coli. Expression_system_taxid: 562.

Biol. unit:

Dimer (from PQS)

Resolution:

1.80Å R-factor: 0.160

Authors:

K.Huang,W.Lu,S.Anderson,M.Laskowski Jr.,M.N.G.James

Key ref:

K.Huang et al. Recruitment of a buried k+ ion to stabilize the negative charge of ionized p1 in the hydrophobic pocket: crystal structures of glu18, Gln18, Asp18 and asn18 variants of turkey ovomucoid inhibitor third domain complexed with streptomyces griseus protease b at various ph's. To be published,

Date:

25-Mar-99 Release date: 26-Aug-03

Protein chain

P00777  (PRTB_STRGR) -  Streptogrisin-B

Seq: 299 a.a.

Struc: 185 a.a.

Protein chain

P68390  (IOVO_MELGA) -  Ovomucoid

Seq: 185 a.a.

Struc: 51 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: Chain E: E.C.3.4.21.81 - Streptogrisin B.
• Reaction: Hydrolysis of proteins with specificity similar to trypsin.

 Gene Ontology (GO) functional annotation 

• Biological process: proteolysis (1 term)
• Biochemical function: catalytic activity (3 terms)