PDBsum entry 1sem

Go to PDB code: 
protein ligands Protein-protein interface(s) links
Signal transduction protein PDB id
Protein chains
58 a.a. *
Waters ×67
* Residue conservation analysis
PDB id:
Name: Signal transduction protein
Title: Structural determinants of peptide-binding orientation and of sequence specificity in sh3 domains
Structure: Sem-5. Chain: a, b. Engineered: yes. 10-residue proline-rich peptide from msos (ace- pro-pro-pro-val-pro-pro-arg-arg-arg). Chain: c, d
Source: Caenorhabditis elegans. Organism_taxid: 6239. Gene: grb2. Expressed in: escherichia coli. Expression_system_taxid: 562. Mus musculus. House mouse. Organism_taxid: 10090
Biol. unit: Dimer (from PQS)
2.00Å     R-factor:   0.189    
Authors: W.A.Lim,F.M.Richards,R.O.Fox
Key ref: W.A.Lim et al. (1994). Structural determinants of peptide-binding orientation and of sequence specificity in SH3 domains. Nature, 372, 375-379. PubMed id: 7802869
28-Mar-95     Release date:   10-Jul-95    
Go to PROCHECK summary

Protein chains
Pfam   ArchSchema ?
P29355  (SEM5_CAEEL) -  Sex muscle abnormal protein 5
228 a.a.
58 a.a.
Key:    PfamA domain  Secondary structure  CATH domain


Nature 372:375-379 (1994)
PubMed id: 7802869  
Structural determinants of peptide-binding orientation and of sequence specificity in SH3 domains.
W.A.Lim, F.M.Richards, R.O.Fox.
The Src-homology-3 (SH3) domains of the Caenorhabditis elegans protein SEM-5 and its human and Drosophila homologues, Grb2 and Drk (refs 1-4), bind proline-rich sequences found in the nucleotide-exchange factor Sos as part of their proposed function linking receptor tyrosine kinase activation to Ras activation. Here we report the crystal structure at 2.0 A resolution of the carboxy-terminal SH3 domain from SEM-5 complexed to the mSos-derived amino-acid sequence PPPVPPRRR. The peptide is found to bind in an orientation ('minus') that is precisely opposite to that observed previously ('plus' orientation) in other SH3-peptide complexes. This novel ability of peptide-recognition proteins to recognize peptides in two distinct modes may play an important role in the signalling specificity of pathways involving SH3 domains. Comparison of this structure with other SH3 complexes reveals how a conserved binding face can be used to recognize peptides in different orientations, and why the Sos peptide binds in this particular orientation.

Literature references that cite this PDB file's key reference

  PubMed id Reference
21472810 C.B.McDonald, K.L.Seldeen, B.J.Deegan, V.Bhat, and A.Farooq (2011).
Binding of the cSH3 domain of Grb2 adaptor to two distinct RXXK motifs within Gab1 docker employs differential mechanisms.
  J Mol Recognit, 24, 585-596.  
21525870 D.Gfeller, F.Butty, M.Wierzbicka, E.Verschueren, P.Vanhee, H.Huang, A.Ernst, N.Dar, I.Stagljar, L.Serrano, S.S.Sidhu, G.D.Bader, and P.M.Kim (2011).
The multiple-specificity landscape of modular peptide recognition domains.
  Mol Syst Biol, 7, 484.  
20005866 C.B.McDonald, K.L.Seldeen, B.J.Deegan, V.Bhat, and A.Farooq (2010).
Assembly of the Sos1-Grb2-Gab1 ternary signaling complex is under allosteric control.
  Arch Biochem Biophys, 494, 216-225.  
19921743 C.Rubini, P.Ruzza, M.R.Spaller, G.Siligardi, R.Hussain, D.G.Udugamasooriya, M.Bellanda, S.Mammi, A.Borgogno, A.Calderan, L.Cesaro, A.M.Brunati, and A.Donella-Deana (2010).
Recognition of lysine-rich peptide ligands by murine cortactin SH3 domain: CD, ITC, and NMR studies.
  Biopolymers, 94, 298-306.  
20974920 F.Alte, A.Stengel, J.P.Benz, E.Petersen, J.Soll, M.Groll, and B.Bölter (2010).
Ferredoxin:NADPH oxidoreductase is recruited to thylakoids by binding to a polyproline type II helix in a pH-dependent manner.
  Proc Natl Acad Sci U S A, 107, 19260-19265.
PDB code: 3mhp
20853307 J.Cai, R.Ou, Y.S.Xu, L.Yang, Z.Lin, and M.Shu (2010).
Modeling and predicting interactions between the human amphiphysin SH3 domains and their peptide ligands based on amino acid information.
  J Pept Sci, 16, 627-632.  
20585582 K.F.Ahmad, and W.A.Lim (2010).
The minimal autoinhibited unit of the guanine nucleotide exchange factor intersectin.
  PLoS One, 5, e11291.
PDB code: 3jv3
20031970 K.X.Zhang, and B.F.Ouellette (2010).
Pandora, a pathway and network discovery approach based on common biological evidence.
  Bioinformatics, 26, 529-535.  
21098279 O.Aitio, M.Hellman, A.Kazlauskas, D.F.Vingadassalom, J.M.Leong, K.Saksela, and P.Permi (2010).
Recognition of tandem PxxP motifs as a unique Src homology 3-binding mode triggers pathogen-driven actin assembly.
  Proc Natl Acad Sci U S A, 107, 21743-21748.
PDB code: 2kxc
19919182 R.Batra-Safferling, J.Granzin, S.Mödder, S.Hoffmann, and D.Willbold (2010).
Structural studies of the phosphatidylinositol 3-kinase (PI3K) SH3 domain in complex with a peptide ligand: role of the anchor residue in ligand binding.
  Biol Chem, 391, 33-42.
PDB codes: 3i5r 3i5s
20024074 S.Hoffmann, S.A.Funke, K.Wiesehan, S.Moedder, J.M.Glück, S.Feuerstein, M.Gerdts, J.Mötter, and D.Willbold (2010).
Competitively selected protein ligands pay their increase in specificity by a decrease in affinity.
  Mol Biosyst, 6, 116-123.  
19397330 A.Manson, S.T.Whitten, J.C.Ferreon, R.O.Fox, and V.J.Hilser (2009).
Characterizing the role of ensemble modulation in mutation-induced changes in binding affinity.
  J Am Chem Soc, 131, 6785-6793.  
19323566 C.B.McDonald, K.L.Seldeen, B.J.Deegan, and A.Farooq (2009).
SH3 domains of Grb2 adaptor bind to PXpsiPXR motifs within the Sos1 nucleotide exchange factor in a discriminate manner.
  Biochemistry, 48, 4074-4085.  
19197237 C.Neufeld, F.V.Filipp, B.Simon, A.Neuhaus, N.Schüller, C.David, H.Kooshapur, T.Madl, R.Erdmann, W.Schliebs, M.Wilmanns, and M.Sattler (2009).
Structural basis for competitive interactions of Pex14 with the import receptors Pex5 and Pex19.
  EMBO J, 28, 745-754.
PDB codes: 2w84 2w85
19590096 E.J.Stollar, B.Garcia, P.A.Chong, A.Rath, H.Lin, J.D.Forman-Kay, and A.R.Davidson (2009).
Structural, functional, and bioinformatic studies demonstrate the crucial role of an extended peptide binding site for the SH3 domain of yeast Abp1p.
  J Biol Chem, 284, 26918-26927.
PDB code: 2rpn
19909364 J.Zhang, B.Dong, and K.A.Siminovitch (2009).
Contributions of Wiskott-Aldrich syndrome family cytoskeletal regulatory adapters to immune regulation.
  Immunol Rev, 232, 175-194.  
19208164 K.X.Zhang, and B.F.Ouellette (2009).
GAIA: a gram-based interaction analysis tool--an approach for identifying interacting domains in yeast.
  BMC Bioinformatics, 10, S60.  
19888460 N.M.Levinson, P.R.Visperas, and J.Kuriyan (2009).
The tyrosine kinase Csk dimerizes through Its SH3 domain.
  PLoS One, 4, e7683.  
19432457 S.P.Edmondson, J.Turri, K.Smith, A.Clark, and J.W.Shriver (2009).
Structure, stability, and flexibility of ribosomal protein L14e from Sulfolobus solfataricus.
  Biochemistry, 48, 5553-5562.  
19137598 S.Tong, H.Zhou, Y.Gao, Z.Zhu, X.Zhang, M.Teng, and L.Niu (2009).
Crystal structure of human osteoclast stimulating factor.
  Proteins, 75, 245-251.
PDB codes: 3ehq 3ehr
19023120 T.Hou, Z.Xu, W.Zhang, W.A.McLaughlin, D.A.Case, Y.Xu, and W.Wang (2009).
Characterization of domain-peptide interaction interface: a generic structure-based model to decipher the binding specificity of SH3 domains.
  Mol Cell Proteomics, 8, 639-649.  
18786926 C.Matsui, S.Kaieda, T.Ikegami, and Y.Mimori-Kiyosue (2008).
Identification of a Link between the SAMP Repeats of Adenomatous Polyposis Coli Tumor Suppressor and the Src Homology 3 Domain of DDEF.
  J Biol Chem, 283, 33006-33020.  
18165234 L.S.Heikkinen, A.Kazlauskas, K.Melén, R.Wagner, T.Ziegler, I.Julkunen, and K.Saksela (2008).
Avian and 1918 Spanish influenza a virus NS1 proteins bind to Crk/CrkL Src homology 3 domains to activate host cell signaling.
  J Biol Chem, 283, 5719-5727.  
18814309 P.Zhou, F.Tian, X.Chen, and Z.Shang (2008).
Modeling and prediction of binding affinities between the human amphiphysin SH3 domain and its peptide ligands using genetic algorithm-Gaussian processes.
  Biopolymers, 90, 792-802.  
18275817 R.A.Robinson, X.Lu, E.Y.Jones, and C.Siebold (2008).
Biochemical and structural studies of ASPP proteins reveal differential binding to p53, p63, and p73.
  Structure, 16, 259-268.
PDB code: 2vge
18577755 S.T.Whitten, H.W.Yang, R.O.Fox, and V.J.Hilser (2008).
Exploring the impact of polyproline II (PII) conformational bias on the binding of peptides to the SEM-5 SH3 domain.
  Protein Sci, 17, 1200-1211.  
17178840 D.Adolph, N.Flach, K.Mueller, D.H.Ostareck, and A.Ostareck-Lederer (2007).
Deciphering the cross talk between hnRNP K and c-Src: the c-Src activation domain in hnRNP K is distinct from a second interaction site.
  Mol Cell Biol, 27, 1758-1770.  
17539817 J.K.Yin, Y.M.Liang, X.L.He, J.G.Lu, L.Zhang, G.Q.Bao, and Q.J.Ma (2007).
Fusion protein containing SH3 domain of c-Abl induces hepatocarcinoma cells to apoptosis.
  Hepatol Res, 37, 454-463.  
17652093 J.M.Janz, T.P.Sakmar, and K.C.Min (2007).
A novel interaction between atrophin-interacting protein 4 and beta-p21-activated kinase-interactive exchange factor is mediated by an SH3 domain.
  J Biol Chem, 282, 28893-28903.
PDB code: 2p4r
17686488 M.Meiyappan, G.Birrane, and J.A.Ladias (2007).
Structural basis for polyproline recognition by the FE65 WW domain.
  J Mol Biol, 372, 970-980.
PDB codes: 2ho2 2idh 2oei
17227859 N.Kannan, N.Haste, S.S.Taylor, and A.F.Neuwald (2007).
The hallmark of AGC kinase functional divergence is its C-terminal tail, a cis-acting regulatory module.
  Proc Natl Acad Sci U S A, 104, 1272-1277.  
17189480 Q.Wang, M.A.Deloia, Y.Kang, C.Litchke, N.Zhang, M.A.Titus, and K.J.Walters (2007).
The SH3 domain of a M7 interacts with its C-terminal proline-rich region.
  Protein Sci, 16, 189-196.
PDB code: 2i0n
17327393 S.Chen, S.Brier, T.E.Smithgall, and J.R.Engen (2007).
The Abl SH2-kinase linker naturally adopts a conformation competent for SH3 domain binding.
  Protein Sci, 16, 572-581.  
17918937 S.J.Wrenn, R.M.Weisinger, D.R.Halpin, and P.B.Harbury (2007).
Synthetic ligands discovered by in vitro selection.
  J Am Chem Soc, 129, 13137-13143.  
17094785 A.Sanjay, T.Miyazaki, C.Itzstein, E.Purev, W.C.Horne, and R.Baron (2006).
Identification and functional characterization of an Src homology domain 3 domain-binding site on Cbl.
  FEBS J, 273, 5442-5456.  
16983070 C.Gu, S.G.Tangye, X.Sun, Y.Luo, Z.Lin, and J.Wu (2006).
The X-linked lymphoproliferative disease gene product SAP associates with PAK-interacting exchange factor and participates in T cell activation.
  Proc Natl Acad Sci U S A, 103, 14447-14452.  
16870929 E.Ferraro, A.Via, G.Ausiello, and M.Helmer-Citterich (2006).
A novel structure-based encoding for machine-learning applied to the inference of SH3 domain specificity.
  Bioinformatics, 22, 2333-2339.  
17020880 G.Moncalián, N.Cárdenes, Y.L.Deribe, M.Spínola-Amilibia, I.Dikic, and J.Bravo (2006).
Atypical polyproline recognition by the CMS N-terminal Src homology 3 domain.
  J Biol Chem, 281, 38845-38853.
PDB codes: 2j6f 2j6k 2j6o 2j7i
16891373 H.X.Zhou (2006).
Quantitative relation between intermolecular and intramolecular binding of pro-rich peptides to SH3 domains.
  Biophys J, 91, 3170-3181.  
16766517 K.Ma, J.G.Forbes, G.Gutierrez-Cruz, and K.Wang (2006).
Titin as a giant scaffold for integrating stress and Src homology domain 3-mediated signaling pathways: the clustering of novel overlap ligand motifs in the elastic PEVK segment.
  J Biol Chem, 281, 27539-27556.  
16446784 T.Hou, K.Chen, W.A.McLaughlin, B.Lu, and W.Wang (2006).
Computational analysis and prediction of the binding motif and protein interacting partners of the Abl SH3 domain.
  PLoS Comput Biol, 2, e1.  
16531238 V.Kanelis, M.C.Bruce, N.R.Skrynnikov, D.Rotin, and J.D.Forman-Kay (2006).
Structural determinants for high-affinity binding in a Nedd4 WW3* domain-Comm PY motif complex.
  Structure, 14, 543-553.
PDB code: 2ez5
16857672 X.Li, Y.Chen, Y.Liu, J.Gao, F.Gao, M.Bartlam, J.Y.Wu, and Z.Rao (2006).
Structural basis of Robo proline-rich motif recognition by the srGAP1 Src homology 3 domain in the Slit-Robo signaling pathway.
  J Biol Chem, 281, 28430-28437.
PDB code: 2gnc
15700296 A.Rath, A.R.Davidson, and C.M.Deber (2005).
The structure of "unstructured" regions in peptides and proteins: role of the polyproline II helix in protein folding and recognition.
  Biopolymers, 80, 179-185.  
15723349 A.le Maire, T.Weber, S.Saunier, I.Broutin, C.Antignac, A.Ducruix, and F.Dardel (2005).
Solution NMR structure of the SH3 domain of human nephrocystin and analysis of a mutation-causing juvenile nephronophthisis.
  Proteins, 59, 347-355.
PDB code: 1s1n
16228008 D.Jozic, N.Cárdenes, Y.L.Deribe, G.Moncalián, D.Hoeller, Y.Groemping, I.Dikic, K.Rittinger, and J.Bravo (2005).
Cbl promotes clustering of endocytic adaptor proteins.
  Nat Struct Mol Biol, 12, 972-979.
PDB codes: 2ak5 2bz8
16210316 E.C.Lerner, R.P.Trible, A.P.Schiavone, J.M.Hochrein, J.R.Engen, and T.E.Smithgall (2005).
Activation of the Src family kinase Hck without SH3-linker release.
  J Biol Chem, 280, 40832-40837.  
16351739 E.Ferraro, A.Via, G.Ausiello, and M.Helmer-Citterich (2005).
A neural strategy for the inference of SH3 domain-peptide interaction specificity.
  BMC Bioinformatics, 6, S13.  
16155203 F.Bauer, K.Schweimer, H.Meiselbach, S.Hoffmann, P.Rösch, and H.Sticht (2005).
Structural characterization of Lyn-SH3 domain in complex with a herpesviral protein reveals an extended recognition motif that enhances binding affinity.
  Protein Sci, 14, 2487-2498.
PDB code: 1w1f
15767257 J.C.Ferreon, A.C.Ferreon, K.Li, and S.M.Lemon (2005).
Molecular determinants of TRIF proteolysis mediated by the hepatitis C virus NS3/4A protease.
  J Biol Chem, 280, 20483-20492.  
15952887 L.Funke, S.Dakoji, and D.S.Bredt (2005).
Membrane-associated guanylate kinases regulate adhesion and plasticity at cell junctions.
  Annu Rev Biochem, 74, 219-245.  
15880548 L.J.Ball, R.Kühne, J.Schneider-Mergener, and H.Oschkinat (2005).
Recognition of Proline-Rich Motifs by Protein-Protein-Interaction Domains.
  Angew Chem Int Ed Engl, 44, 2852-2869.  
16000375 M.C.Brown, L.A.Cary, J.S.Jamieson, J.A.Cooper, and C.E.Turner (2005).
Src and FAK kinases cooperate to phosphorylate paxillin kinase linker, stimulate its focal adhesion localization, and regulate cell spreading and protrusiveness.
  Mol Biol Cell, 16, 4316-4328.  
16207360 R.Riley, C.Lee, C.Sabatti, and D.Eisenberg (2005).
Inferring protein domain interactions from databases of interacting proteins.
  Genome Biol, 6, R89.  
16313344 W.C.Horne, A.Sanjay, A.Bruzzaniti, and R.Baron (2005).
The role(s) of Src kinase and Cbl proteins in the regulation of osteoclast differentiation and function.
  Immunol Rev, 208, 106-125.  
14737190 C.Landgraf, S.Panni, L.Montecchi-Palazzi, L.Castagnoli, J.Schneider-Mergener, R.Volkmer-Engert, and G.Cesareni (2004).
Protein interaction networks by proteome peptide scanning.
  PLoS Biol, 2, E14.  
15526038 H.Dvir, M.Harel, S.Bon, W.Q.Liu, M.Vidal, C.Garbay, J.L.Sussman, J.Massoulié, and I.Silman (2004).
The synaptic acetylcholinesterase tetramer assembles around a polyproline II helix.
  EMBO J, 23, 4394-4405.
PDB code: 1vzj
15200958 J.A.Marles, S.Dahesh, J.Haynes, B.J.Andrews, and A.R.Davidson (2004).
Protein-protein interaction affinity plays a crucial role in controlling the Sho1p-mediated signal transduction pathway in yeast.
  Mol Cell, 14, 813-823.  
15541654 K.Badour, J.Zhang, and K.A.Siminovitch (2004).
Involvement of the Wiskott-Aldrich syndrome protein and other actin regulatory adaptors in T cell activation.
  Semin Immunol, 16, 395-407.  
14978303 M.R.Ferguson, X.Fan, M.Mukherjee, J.Luo, R.Khan, J.C.Ferreon, V.J.Hilser, R.E.Shope, and R.O.Fox (2004).
Directed discovery of bivalent peptide ligands to an SH3 domain.
  Protein Sci, 13, 626-632.  
15147273 S.Yuzawa, N.N.Suzuki, Y.Fujioka, K.Ogura, H.Sumimoto, and F.Inagaki (2004).
A molecular mechanism for autoinhibition of the tandem SH3 domains of p47phox, the regulatory subunit of the phagocyte NADPH oxidase.
  Genes Cells, 9, 443-456.
PDB code: 1uec
15133021 Y.Kato, K.Nagata, M.Takahashi, L.Lian, J.J.Herrero, M.Sudol, and M.Tanokura (2004).
Common mechanism of ligand recognition by group II/III WW domains: redefining their functional classification.
  J Biol Chem, 279, 31833-31841.  
14668868 A.Zarrinpar, S.H.Park, and W.A.Lim (2003).
Optimization of specificity in a cellular protein interaction network by negative selection.
  Nature, 426, 676-680.  
12833568 F.Santamaria, Z.Wu, C.Boulègue, G.Pál, and W.Lu (2003).
Reexamination of the recognition preference of the specificity pocket of the Abl SH3 domain.
  J Mol Recognit, 16, 131-138.  
12592015 J.C.Ferreon, and V.J.Hilser (2003).
The effect of the polyproline II (PPII) conformation on the denatured state entropy.
  Protein Sci, 12, 447-457.  
12717021 J.C.Ferreon, and V.J.Hilser (2003).
Ligand-induced changes in dynamics in the RT loop of the C-terminal SH3 domain of Sem-5 indicate cooperative conformational coupling.
  Protein Sci, 12, 982-996.  
12670398 K.Badour, J.Zhang, and K.A.Siminovitch (2003).
The Wiskott-Aldrich syndrome protein: forging the link between actin and cell activation.
  Immunol Rev, 192, 98.  
12773374 M.Harkiolaki, M.Lewitzky, R.J.Gilbert, E.Y.Jones, R.P.Bourette, G.Mouchiroud, H.Sondermann, I.Moarefi, and S.M.Feller (2003).
Structural basis for SH3 domain-mediated high-affinity binding between Mona/Gads and SLP-76.
  EMBO J, 22, 2571-2582.
PDB code: 1oeb
12654927 P.C.Henry, V.Kanelis, M.C.O'Brien, B.Kim, I.Gautschi, J.Forman-Kay, L.Schild, and D.Rotin (2003).
Affinity and specificity of interactions between Nedd4 isoforms and the epithelial Na+ channel.
  J Biol Chem, 278, 20019-20028.  
12620234 Q.Liu, D.Berry, P.Nash, T.Pawson, C.J.McGlade, and S.S.Li (2003).
Structural basis for specific binding of the Gads SH3 domain to an RxxK motif-containing SLP-76 peptide: a novel mode of peptide recognition.
  Mol Cell, 11, 471-481.
PDB code: 1h3h
12767128 S.L.Lam, and V.L.Hsu (2003).
NMR identification of left-handed polyproline type II helices.
  Biopolymers, 69, 270-281.  
12688310 S.M.Feller, G.Tuchscherer, and J.Voss (2003).
High affinity molecules disrupting GRB2 protein complexes as a therapeutic strategy for chronic myelogenous leukaemia.
  Leuk Lymphoma, 44, 411-427.  
13129930 T.Kaneko, T.Kumasaka, T.Ganbe, T.Sato, K.Miyazawa, N.Kitamura, and N.Tanaka (2003).
Structural insight into modest binding of a non-PXXP ligand to the signal transducing adaptor molecule-2 Src homology 3 domain.
  J Biol Chem, 278, 48162-48168.
PDB code: 1uj0
12395395 B.Bochicchio, and A.M.Tamburro (2002).
Polyproline II structure in proteins: identification by chiroptical spectroscopies, stability, and functions.
  Chirality, 14, 782-792.  
12437929 B.F.Volkman, K.E.Prehoda, J.A.Scott, F.C.Peterson, and W.A.Lim (2002).
Structure of the N-WASP EVH1 domain-WIP complex: insight into the molecular basis of Wiskott-Aldrich Syndrome.
  Cell, 111, 565-576.
PDB code: 1mke
11668184 B.Fazi, M.J.Cope, A.Douangamath, S.Ferracuti, K.Schirwitz, A.Zucconi, D.G.Drubin, M.Wilmanns, G.Cesareni, and L.Castagnoli (2002).
Unusual binding properties of the SH3 domain of the yeast actin-binding protein Abp1: structural and functional analysis.
  J Biol Chem, 277, 5290-5298.
PDB code: 1jo8
12169629 K.Kami, R.Takeya, H.Sumimoto, and D.Kohda (2002).
Diverse recognition of non-PxxP peptide ligands by the SH3 domains from p67(phox), Grb2 and Pex13p.
  EMBO J, 21, 4268-4276.
PDB code: 1k4u
12384576 L.W.Donaldson, G.Gish, T.Pawson, L.E.Kay, and J.D.Forman-Kay (2002).
Structure of a regulatory complex involving the Abl SH3 domain, the Crk SH2 domain, and a Crk-derived phosphopeptide.
  Proc Natl Acad Sci U S A, 99, 14053-14058.
PDB code: 1ju5
11877742 M.P.Okoh, and M.Vihinen (2002).
Interaction between Btk TH and SH3 domain.
  Biopolymers, 63, 325-334.  
11929862 S.Panni, L.Dente, and G.Cesareni (2002).
In vitro evolution of recognition specificity mediated by SH3 domains reveals target recognition rules.
  J Biol Chem, 277, 21666-21674.  
12186888 X.Jiang, Z.Hanna, M.Kaouass, L.Girard, and P.Jolicoeur (2002).
Ahi-1, a novel gene encoding a modular protein with WD40-repeat and SH3 domains, is targeted by the Ahi-1 and Mis-2 provirus integrations.
  J Virol, 76, 9046-9059.  
11779504 A.W.McGee, S.R.Dakoji, O.Olsen, D.S.Bredt, W.A.Lim, and K.E.Prehoda (2001).
Structure of the SH3-guanylate kinase module from PSD-95 suggests a mechanism for regulated assembly of MAGUK scaffolding proteins.
  Mol Cell, 8, 1291-1301.
PDB code: 1kjw
11551507 C.L.Kielkopf, N.A.Rodionova, M.R.Green, and S.K.Burley (2001).
A novel peptide recognition mode revealed by the X-ray structure of a core U2AF35/U2AF65 heterodimer.
  Cell, 106, 595-605.
PDB code: 1jmt
11500884 G.Tuchscherer, D.Grell, Y.Tatsu, P.Durieux, J.Fernandez-Carneado, B.Hengst, C.Kardinal, and S.Feller (2001).
Targeting Molecular Recognition: Exploring the Dual Role of Functional Pseudoprolines in the Design of SH3 Ligands This work was supported by the Swiss National Science Foundation.
  Angew Chem Int Ed Engl, 40, 2844-2848.  
11518702 H.Korkaya, S.Jameel, D.Gupta, S.Tyagi, R.Kumar, M.Zafrullah, M.Mazumdar, S.K.Lal, L.Xiaofang, D.Sehgal, S.R.Das, and D.Sahal (2001).
The ORF3 protein of hepatitis E virus binds to Src homology 3 domains and activates MAPK.
  J Biol Chem, 276, 42389-42400.  
11331761 J.C.Lougheed, J.M.Holton, T.Alber, J.F.Bazan, and T.M.Handel (2001).
Structure of melanoma inhibitory activity protein, a member of a recently identified family of secreted proteins.
  Proc Natl Acad Sci U S A, 98, 5515-5520.
PDB code: 1i1j
11316885 K.V.Kishan, M.E.Newcomer, T.H.Rhodes, and S.D.Guilliot (2001).
Effect of pH and salt bridges on structural assembly: molecular structures of the monomer and intertwined dimer of the Eps8 SH3 domain.
  Protein Sci, 10, 1046-1055.
PDB codes: 1i07 1i0c
  11463741 M.Geyer, O.T.Fackler, and B.M.Peterlin (2001).
Structure--function relationships in HIV-1 Nef.
  EMBO Rep, 2, 580-585.  
11406576 M.Nishida, K.Nagata, Y.Hachimori, M.Horiuchi, K.Ogura, V.Mandiyan, J.Schlessinger, and F.Inagaki (2001).
Novel recognition mode between Vav and Grb2 SH3 domains.
  EMBO J, 20, 2995-3007.
PDB codes: 1gcp 1gcq
11682324 M.Vidal, V.Gigoux, and C.Garbay (2001).
SH2 and SH3 domains as targets for anti-proliferative agents.
  Crit Rev Oncol Hematol, 40, 175-186.  
11685249 R.Ghose, A.Shekhtman, M.J.Goger, H.Ji, and D.Cowburn (2001).
A novel, specific interaction involving the Csk SH3 domain and its natural ligand.
  Nat Struct Biol, 8, 998.
PDB code: 1jeg
11782293 V.Agrawal, and R.K.Kishan (2001).
Functional evolution of two subtly different (similar) folds.
  BMC Struct Biol, 1, 5.  
  11206067 A.Rath, and A.R.Davidson (2000).
The design of a hyperstable mutant of the Abp1p SH3 domain by sequence alignment analysis.
  Protein Sci, 9, 2457-2469.  
10873829 D.J.Owen, and J.P.Luzio (2000).
Structural insights into clathrin-mediated endocytosis.
  Curr Opin Cell Biol, 12, 467-474.  
  11071920 G.Bottger, P.Barnett, A.T.Klein, A.Kragt, H.F.Tabak, and B.Distel (2000).
Saccharomyces cerevisiae PTS1 receptor Pex5p interacts with the SH3 domain of the peroxisomal membrane protein Pex13p in an unconventional, non-PXXP-related manner.
  Mol Biol Cell, 11, 3963-3976.  
10856234 H.Kang, C.Freund, J.S.Duke-Cohan, A.Musacchio, G.Wagner, and C.E.Rudd (2000).
SH3 domain recognition of a proline-independent tyrosine-based RKxxYxxY motif in immune cell adaptor SKAP55.
  EMBO J, 19, 2889-2899.  
10662684 J.E.Ladbury, and S.Arold (2000).
Searching for specificity in SH domains.
  Chem Biol, 7, R3-R8.  
10931866 K.Oegema, M.S.Savoian, T.J.Mitchison, and C.M.Field (2000).
Functional analysis of a human homologue of the Drosophila actin binding protein anillin suggests a role in cytokinesis.
  J Cell Biol, 150, 539-552.  
10723797 L.A.Norian, and G.A.Koretzky (2000).
Intracellular adapter molecules.
  Semin Immunol, 12, 43-54.  
10930849 M.Barzik, W.D.Schubert, U.Carl, J.Wehland, and D.W.Heinz (2000).
Crystallization and preliminary X-ray analysis of the EVH1 domain of Vesl-2b.
  Acta Crystallogr D Biol Crystallogr, 56, 930-932.  
10744724 M.T.Bedford, D.Sarbassova, J.Xu, P.Leder, and M.B.Yaffe (2000).
A novel pro-Arg motif recognized by WW domains.
  J Biol Chem, 275, 10359-10369.  
10861385 N.Okishio, M.Nagai, R.Fukuda, S.Nagatomo, and T.Kitagawa (2000).
Interactions of phosphatidylinositol 3-kinase Src homology 3 domain with its ligand peptide studied by absorption, circular dichroism, and UV resonance raman spectroscopies.
  Biopolymers, 57, 208-217.  
11063574 N.Schiering, E.Casale, P.Caccia, P.Giordano, and C.Battistini (2000).
Dimer formation through domain swapping in the crystal structure of the Grb2-SH2-Ac-pYVNV complex.
  Biochemistry, 39, 13376-13382.
PDB code: 1fyr
11206058 P.A.Dalby, R.H.Hoess, and W.F.DeGrado (2000).
Evolution of binding affinity in a WW domain probed by phage display.
  Protein Sci, 9, 2366-2376.  
11101511 P.Barnett, G.Bottger, A.T.Klein, H.F.Tabak, and B.Distel (2000).
The peroxisomal membrane protein Pex13p shows a novel mode of SH3 interaction.
  EMBO J, 19, 6382-6391.  
10933808 R.Kleene, B.Classen, J.Zdzieblo, and M.Schrader (2000).
SH3 binding sites of ZG29p mediate an interaction with amylase and are involved in condensation-sorting in the exocrine rat pancreas.
  Biochemistry, 39, 9893-9900.  
  11152127 S.M.Larson, and A.R.Davidson (2000).
The identification of conserved interactions within the SH3 domain by alignment of sequences and structures.
  Protein Sci, 9, 2170-2180.  
10716926 X.K.Tong, N.K.Hussain, Heuvel, A.Kurakin, E.Abi-Jaoude, C.C.Quinn, M.F.Olson, R.Marais, D.Baranes, B.K.Kay, and P.S.McPherson (2000).
The endocytic protein intersectin is a major binding partner for the Ras exchange factor mSos1 in rat brain.
  EMBO J, 19, 1263-1271.  
10611245 Y.T.Yan, S.M.Stein, J.Ding, M.M.Shen, and C.Abate-Shen (2000).
A novel PF/PN motif inhibits nuclear localization and DNA binding activity of the ESX1 homeoprotein.
  Mol Cell Biol, 20, 661-671.  
10805734 Z.S.Zhao, E.Manser, and L.Lim (2000).
Interaction between PAK and nck: a template for Nck targets and role of PAK autophosphorylation.
  Mol Cell Biol, 20, 3906-3917.  
10508163 A.M.Mongioví, P.R.Romano, S.Panni, M.Mendoza, W.T.Wong, A.Musacchio, G.Cesareni, and P.P.Di Fiore (1999).
A novel peptide-SH3 interaction.
  EMBO J, 18, 5300-5309.  
10467125 B.Aghazadeh, and M.K.Rosen (1999).
Ligand recognition by SH3 and WW domains: the role of N-alkylation in PPII helices.
  Chem Biol, 6, R241-R246.  
  10022919 F.J.King, E.Hu, D.F.Harris, P.Sarraf, B.M.Spiegelman, and T.M.Roberts (1999).
DEF-1, a novel Src SH3 binding protein that promotes adipogenesis in fibroblastic cell lines.
  Mol Cell Biol, 19, 2330-2337.  
10542231 G.Cestra, L.Castagnoli, L.Dente, O.Minenkova, A.Petrelli, N.Migone, U.Hoffmüller, J.Schneider-Mergener, and G.Cesareni (1999).
The SH3 domains of endophilin and amphiphysin bind to the proline-rich region of synaptojanin 1 at distinct sites that display an unconventional binding specificity.
  J Biol Chem, 274, 32001-32007.  
10339551 G.Wang, G.P.Wylie, P.D.Twigg, D.L.Caspar, J.R.Murphy, and T.M.Logan (1999).
Solution structure and peptide binding studies of the C-terminal src homology 3-like domain of the diphtheria toxin repressor protein.
  Proc Natl Acad Sci U S A, 96, 6119-6124.
PDB code: 1bym
10518213 H.Ye, Y.C.Park, M.Kreishman, E.Kieff, and H.Wu (1999).
The structural basis for the recognition of diverse receptor sequences by TRAF2.
  Mol Cell, 4, 321-330.
PDB codes: 1czy 1czz 1d00 1d01 1d0a 1d0j
10338211 K.E.Prehoda, D.J.Lee, and W.A.Lim (1999).
Structure of the enabled/VASP homology 1 domain-peptide complex: a key component in the spatial control of actin assembly.
  Cell, 97, 471-480.
PDB code: 1evh
10393272 L.Buday (1999).
Membrane-targeting of signalling molecules by SH2/SH3 domain-containing adaptor proteins.
  Biochim Biophys Acta, 1422, 187-204.  
10491105 R.P.Menon, and R.C.Hughes (1999).
Determinants in the N-terminal domains of galectin-3 for secretion by a novel pathway circumventing the endoplasmic reticulum-Golgi complex.
  Eur J Biochem, 264, 569-576.  
10535922 S.Hirotsu, Y.Abe, K.Okada, N.Nagahara, H.Hori, T.Nishino, and T.Hakoshima (1999).
Crystal structure of a multifunctional 2-Cys peroxiredoxin heme-binding protein 23 kDa/proliferation-associated gene product.
  Proc Natl Acad Sci U S A, 96, 12333-12338.
PDB code: 1qq2
10492401 Shivakrupa, R.Singh, and G.Swarup (1999).
Identification of a novel splice variant of C3G which shows tissue-specific expression.
  DNA Cell Biol, 18, 701-708.  
10559253 T.Ago, H.Nunoi, T.Ito, and H.Sumimoto (1999).
Mechanism for phosphorylation-induced activation of the phagocyte NADPH oxidase protein p47(phox). Triple replacement of serines 303, 304, and 328 with aspartates disrupts the SH3 domain-mediated intramolecular interaction in p47(phox), thereby activating the oxidase.
  J Biol Chem, 274, 33644-33653.  
10595512 W.Ruan, P.Pang, and Y.Rao (1999).
The SH2/SH3 adaptor protein dock interacts with the Ste20-like kinase misshapen in controlling growth cone motility.
  Neuron, 24, 595-605.  
10593988 Y.Mochizuki, and T.Takenawa (1999).
Novel inositol polyphosphate 5-phosphatase localizes at membrane ruffles.
  J Biol Chem, 274, 36790-36795.  
9582365 A.Gregorieff, J.F.Cloutier, and A.Veillette (1998).
Sequence requirements for association of protein-tyrosine phosphatase PEP with the Src homology 3 domain of inhibitory tyrosine protein kinase p50(csk).
  J Biol Chem, 273, 13217-13222.  
  9418863 A.R.Comer, S.M.Ahern-Djamali, J.L.Juang, P.D.Jackson, and F.M.Hoffmann (1998).
Phosphorylation of Enabled by the Drosophila Abelson tyrosine kinase regulates the in vivo function and protein-protein interactions of Enabled.
  Mol Cell Biol, 18, 152-160.  
9500553 D.Barilá, and G.Superti-Furga (1998).
An intramolecular SH3-domain interaction regulates c-Abl activity.
  Nat Genet, 18, 280-282.  
9736607 D.J.Owen, P.Wigge, Y.Vallis, J.D.Moore, P.R.Evans, and H.T.McMahon (1998).
Crystal structure of the amphiphysin-2 SH3 domain and its role in the prevention of dynamin ring formation.
  EMBO J, 17, 5273-5285.
PDB code: 1bb9
9485443 H.Hansson, P.T.Mattsson, P.Allard, P.Haapaniemi, M.Vihinen, C.I.Smith, and T.Hard (1998).
Solution structure of the SH3 domain from Bruton's tyrosine kinase.
  Biochemistry, 37, 2912-2924.
PDB codes: 1aww 1awx
  9621042 I.H.Khan, E.T.Sawai, E.Antonio, C.J.Weber, C.P.Mandell, P.Montbriand, and P.A.Luciw (1998).
Role of the SH3-ligand domain of simian immunodeficiency virus Nef in interaction with Nef-associated kinase and simian AIDS in rhesus macaques.
  J Virol, 72, 5820-5830.  
9612082 J.C.Williams, R.K.Wierenga, and M.Saraste (1998).
Insights into Src kinase functions: structural comparisons.
  Trends Biochem Sci, 23, 179-184.  
  9475731 J.Collet, C.A.Spike, E.A.Lundquist, J.E.Shaw, and R.K.Herman (1998).
Analysis of osm-6, a gene that affects sensory cilium structure and sensory neuron function in Caenorhabditis elegans.
  Genetics, 148, 187-200.  
10417854 J.Dorrestijn, F.J.van Bussel, J.A.Maassen, and D.S.Gomes de Mesquita (1998).
Early steps in insulin action.
  Arch Physiol Biochem, 106, 269-289.  
9851931 J.T.Nguyen, C.W.Turck, F.E.Cohen, R.N.Zuckermann, and W.A.Lim (1998).
Exploiting the basis of proline recognition by SH3 and WW domains: design of N-substituted inhibitors.
  Science, 282, 2088-2092.
PDB codes: 1b07 2sem 3sem
9748234 K.H.Kirsch, M.M.Georgescu, and H.Hanafusa (1998).
Direct binding of p130(Cas) to the guanine nucleotide exchange factor C3G.
  J Biol Chem, 273, 25673-25679.  
9529254 R.Steven, T.J.Kubiseski, H.Zheng, S.Kulkarni, J.Mancillas, A.Ruiz Morales, C.W.Hogue, T.Pawson, and J.Culotti (1998).
UNC-73 activates the Rac GTPase and is required for cell and growth cone migrations in C. elegans.
  Cell, 92, 785-795.  
9778343 S.Arold, R.O'Brien, P.Franken, M.P.Strub, F.Hoh, C.Dumas, and J.E.Ladbury (1998).
RT loop flexibility enhances the specificity of Src family SH3 domains for HIV-1 Nef.
  Biochemistry, 37, 14683-14691.
PDB code: 1bu1
9593201 S.Knapp, P.T.Mattson, P.Christova, K.D.Berndt, A.Karshikoff, M.Vihinen, C.I.Smith, and R.Ladenstein (1998).
Thermal unfolding of small proteins with SH3 domain folding pattern.
  Proteins, 31, 309-319.  
9799227 S.Simon, E.Krejci, and J.Massoulié (1998).
A four-to-one association between peptide motifs: four C-terminal domains from cholinesterase assemble with one proline-rich attachment domain (PRAD) in the secretory pathway.
  EMBO J, 17, 6178-6187.  
9209158 A.Borroto, M.A.Jiménez, B.Alarcón, and M.Rico (1997).
1H-NMR analysis of CD3-epsilon reveals the presence of turn-helix structures around the ITAM motif in an otherwise random coil cytoplasmic tail.
  Biopolymers, 42, 75-88.  
9182529 C.Leprince, F.Romero, D.Cussac, B.Vayssiere, R.Berger, A.Tavitian, and J.H.Camonis (1997).
A new member of the amphiphysin family connecting endocytosis and signal transduction pathways.
  J Biol Chem, 272, 15101-15105.  
9122235 C.W.So, C.Caldas, M.M.Liu, S.J.Chen, Q.H.Huang, L.J.Gu, M.H.Sham, L.M.Wiedemann, and L.C.Chan (1997).
EEN encodes for a member of a new family of proteins containing an Src homology 3 domain and is the third gene located on chromosome 19p13 that fuses to MLL in human leukemia.
  Proc Natl Acad Sci U S A, 94, 2563-2568.  
9566119 D.C.Dalgarno, M.C.Botfield, and R.J.Rickles (1997).
SH3 domains and drug design: ligands, structure, and biological function.
  Biopolymers, 43, 383-400.  
9434895 F.Sicheri, and J.Kuriyan (1997).
Structures of Src-family tyrosine kinases.
  Curr Opin Struct Biol, 7, 777-785.  
9261721 G.E.Morley, J.F.Ek-Vitorín, S.M.Taffet, and M.Delmar (1997).
Structure of connexin43 and its regulation by pHi.
  J Cardiovasc Electrophysiol, 8, 939-951.  
9408950 H.V.Patel, S.R.Tzeng, C.Y.Liao, S.H.Chen, and J.W.Cheng (1997).
SH3 domain of Bruton's tyrosine kinase can bind to proline-rich peptides of TH domain of the kinase and p120cbl.
  Proteins, 29, 545-552.  
9210364 J.A.Frazier, and C.M.Field (1997).
Actin cytoskeleton: are FH proteins local organizers?
  Curr Biol, 7, R414-R417.  
9241420 J.Kuriyan, and D.Cowburn (1997).
Modular peptide recognition domains in eukaryotic signaling.
  Annu Rev Biophys Biomol Struct, 26, 259-288.  
9095189 J.T.Nguyen, and W.A.Lim (1997).
How Src exercises self-restraint.
  Nat Struct Biol, 4, 256-260.  
9016723 K.S.Thorn, H.E.Christensen, R.Shigeta, D.Huddler, L.Shalaby, U.Lindberg, N.H.Chua, and C.E.Schutt (1997).
The crystal structure of a major allergen from plants.
  Structure, 5, 19-32.
PDB codes: 1a0k 1plm 3nul
9303002 K.V.Kishan, G.Scita, W.T.Wong, P.P.Di Fiore, and M.E.Newcomer (1997).
The SH3 domain of Eps8 exists as a novel intertwined dimer.
  Nat Struct Biol, 4, 739-743.
PDB code: 1aoj
9346925 M.Anafi, F.Kiefer, G.D.Gish, G.Mbamalu, N.N.Iscove, and T.Pawson (1997).
SH2/SH3 adaptor proteins can link tyrosine kinases to a Ste20-related protein kinase, HPK1.
  J Biol Chem, 272, 27804-27811.  
9428519 M.B.Yaffe, K.Rittinger, S.Volinia, P.R.Caron, A.Aitken, H.Leffers, S.J.Gamblin, S.J.Smerdon, and L.C.Cantley (1997).
The structural basis for 14-3-3:phosphopeptide binding specificity.
  Cell, 91, 961-971.
PDB code: 14ps
9326643 M.I.Wahl, A.C.Fluckiger, R.M.Kato, H.Park, O.N.Witte, and D.J.Rawlings (1997).
Phosphorylation of two regulatory tyrosine residues in the activation of Bruton's tyrosine kinase via alternative receptors.
  Proc Natl Acad Sci U S A, 94, 11526-11533.  
9360613 N.M.Mahoney, P.A.Janmey, and S.C.Almo (1997).
Structure of the profilin-poly-L-proline complex involved in morphogenesis and cytoskeletal regulation.
  Nat Struct Biol, 4, 953-960.
PDB code: 1awi
9111080 P.M.Okamoto, J.S.Herskovits, and R.B.Vallee (1997).
Role of the basic, proline-rich region of dynamin in Src homology 3 domain binding and endocytosis.
  J Biol Chem, 272, 11629-11635.  
9108038 P.Xu, K.I.Mitchelhill, B.Kobe, B.E.Kemp, and H.G.Zot (1997).
The myosin-I-binding protein Acan125 binds the SH3 domain and belongs to the superfamily of leucine-rich repeat proteins.
  Proc Natl Acad Sci U S A, 94, 3685-3690.  
9006950 S.Bon, F.Coussen, and J.Massoulié (1997).
Quaternary associations of acetylcholinesterase. II. The polyproline attachment domain of the collagen tail.
  J Biol Chem, 272, 3016-3021.  
  9045850 S.Negrete-Urtasun, S.H.Denison, and H.N.Arst (1997).
Characterization of the pH signal transduction pathway gene palA of Aspergillus nidulans and identification of possible homologs.
  J Bacteriol, 179, 1832-1835.  
9630982 A.B.Sparks, N.G.Hoffman, S.J.McConnell, D.M.Fowlkes, and B.K.Kay (1996).
Cloning of ligand targets: systematic isolation of SH3 domain-containing proteins.
  Nat Biotechnol, 14, 741-744.  
8807882 A.J.Wilkinson (1996).
Accommodating structurally diverse peptides in proteins.
  Chem Biol, 3, 519-524.  
8805554 C.J.Morton, D.J.Pugh, E.L.Brown, J.D.Kahmann, D.A.Renzoni, and I.D.Campbell (1996).
Solution structure and peptide binding of the SH3 domain from human Fyn.
  Structure, 4, 705-714.
PDB codes: 1nyf 1nyg
8570618 C.Tribioli, S.Droetto, S.Bione, G.Cesareni, M.R.Torrisi, L.V.Lotti, L.Lanfrancone, D.Toniolo, and P.Pelicci (1996).
An X chromosome-linked gene encoding a protein with characteristics of a rhoGAP predominantly expressed in hematopoietic cells.
  Proc Natl Acad Sci U S A, 93, 695-699.  
8961927 D.A.Renzoni, D.J.Pugh, G.Siligardi, P.Das, C.J.Morton, C.Rossi, M.D.Waterfield, I.D.Campbell, and J.E.Ladbury (1996).
Structural and thermodynamic characterization of the interaction of the SH3 domain from Fyn with the proline-rich binding site on the p85 subunit of PI3-kinase.
  Biochemistry, 35, 15646-15653.
PDB codes: 1a0n 1azg
8987987 E.C.Petrella, L.M.Machesky, D.A.Kaiser, and T.D.Pollard (1996).
Structural requirements and thermodynamics of the interaction of proline peptides with profilin.
  Biochemistry, 35, 16535-16543.  
  8649363 F.Parker, F.Maurier, I.Delumeau, M.Duchesne, D.Faucher, L.Debussche, A.Dugue, F.Schweighoffer, and B.Tocque (1996).
A Ras-GTPase-activating protein SH3-domain-binding protein.
  Mol Cell Biol, 16, 2561-2569.  
8647900 G.Weskamp, J.Krätzschmar, M.S.Reid, and C.P.Blobel (1996).
MDC9, a widely expressed cellular disintegrin containing cytoplasmic SH3 ligand domains.
  J Cell Biol, 132, 717-726.  
8630736 H.Park, M.I.Wahl, D.E.Afar, C.W.Turck, D.J.Rawlings, C.Tam, A.M.Scharenberg, J.P.Kinet, and O.N.Witte (1996).
Regulation of Btk function by a major autophosphorylation site within the SH3 domain.
  Immunity, 4, 515-525.  
  8635453 Mendez, A.G.Adams, R.A.Sokolic, H.L.Malech, and T.L.Leto (1996).
Multiple SH3 domain interactions regulate NADPH oxidase assembly in whole cells.
  EMBO J, 15, 1211-1220.  
8824292 J.A.Donovan, Y.Ota, W.Y.Langdon, and L.E.Samelson (1996).
Regulation of the association of p120cbl with Grb2 in Jurkat T cells.
  J Biol Chem, 271, 26369-26374.  
8874002 J.F.Ek-Vitorín, G.Calero, G.E.Morley, W.Coombs, S.M.Taffet, and M.Delmar (1996).
PH regulation of connexin43: molecular analysis of the gating particle.
  Biophys J, 71, 1273-1284.  
8617717 J.Krätzschmar, L.Lum, and C.P.Blobel (1996).
Metargidin, a membrane-anchored metalloprotease-disintegrin protein with an RGD integrin binding sequence.
  J Biol Chem, 271, 4593-4596.  
8736558 J.Qin, G.M.Clore, W.P.Kennedy, J.Kuszewski, and A.M.Gronenborn (1996).
The solution structure of human thioredoxin complexed with its target from Ref-1 reveals peptide chain reversal.
  Structure, 4, 613-620.
PDB codes: 1cqg 1cqh
8650211 J.Shi, C.R.Ross, T.L.Leto, and F.Blecha (1996).
PR-39, a proline-rich antibacterial peptide that inhibits phagocyte NADPH oxidase activity by binding to Src homology 3 domains of p47 phox.
  Proc Natl Acad Sci U S A, 93, 6014-6018.  
8663276 J.W.Nelson, J.Zhu, C.C.Smith, M.Kulka, and L.Aurelian (1996).
ATP and SH3 binding sites in the protein kinase of the large subunit of herpes simplex virus type 2 of ribonucleotide reductase (ICP10).
  J Biol Chem, 271, 17021-17027.  
  8670803 J.den Hertog, and T.Hunter (1996).
Tight association of GRB2 with receptor protein-tyrosine phosphatase alpha is mediated by the SH2 and C-terminal SH3 domains.
  EMBO J, 15, 3016-3027.  
8702917 M.Anafi, M.K.Rosen, G.D.Gish, L.E.Kay, and T.Pawson (1996).
A potential SH3 domain-binding site in the Crk SH2 domain.
  J Biol Chem, 271, 21365-21374.  
8906616 M.C.Weiler, J.L.Smith, and J.N.Masters (1996).
CR16, a novel proline-rich protein expressed in rat brain neurons, binds to SH3 domains and is a MAP kinase substrate.
  J Mol Neurosci, 7, 203-215.  
  8649366 M.E.Nickas, and M.P.Yaffe (1996).
BRO1, a novel gene that interacts with components of the Pkc1p-mitogen-activated protein kinase pathway in Saccharomyces cerevisiae.
  Mol Cell Biol, 16, 2585-2593.  
8662907 M.Matsuda, S.Ota, R.Tanimura, H.Nakamura, K.Matuoka, T.Takenawa, K.Nagashima, and T.Kurata (1996).
Interaction between the amino-terminal SH3 domain of CRK and its natural target proteins.
  J Biol Chem, 271, 14468-14472.  
8718852 M.T.Pisabarro, and L.Serrano (1996).
Rational design of specific high-affinity peptide ligands for the Abl-SH3 domain.
  Biochemistry, 35, 10634-10640.  
9238627 N.G.Hoffman, A.B.Sparks, J.M.Carter, and B.K.Kay (1996).
Binding properties of SH3 peptide ligands identified from phage-displayed random peptide libraries.
  Mol Divers, 2, 5.  
8799133 P.A.Rejto, and G.M.Verkhivker (1996).
Unraveling principles of lead discovery: from unfrustrated energy landscapes to novel molecular anchors.
  Proc Natl Acad Sci U S A, 93, 8945-8950.  
8807900 S.Feng, T.M.Kapoor, F.Shirai, A.P.Combs, and S.L.Schreiber (1996).
Molecular basis for the binding of SH3 ligands with non-peptide elements identified by combinatorial synthesis.
  Chem Biol, 3, 661-670.
PDB codes: 1nlo 1nlp
8599760 S.Grzesiek, A.Bax, G.M.Clore, A.M.Gronenborn, J.S.Hu, J.Kaufman, I.Palmer, S.J.Stahl, and P.T.Wingfield (1996).
The solution structure of HIV-1 Nef reveals an unexpected fold and permits delineation of the binding surface for the SH3 domain of Hck tyrosine protein kinase.
  Nat Struct Biol, 3, 340-345.
PDB code: 1nef
8805558 W.A.Lim (1996).
Reading between the lines: SH3 recognition of an intact protein.
  Structure, 4, 657-659.  
7890694 A.Fuchs, M.C.Dagher, and P.V.Vignais (1995).
Mapping the domains of interaction of p40phox with both p47phox and p67phox of the neutrophil oxidase complex using the two-hybrid system.
  J Biol Chem, 270, 5695-5697.  
7542990 B.J.Mayer, and M.J.Eck (1995).
SH3 domains. Minding your p's and q's.
  Curr Biol, 5, 364-367.  
  7774577 B.S.Knudsen, J.Zheng, S.M.Feller, J.P.Mayer, S.K.Burrell, D.Cowburn, and H.Hanafusa (1995).
Affinity and specificity requirements for the first Src homology 3 domain of the Crk proteins.
  EMBO J, 14, 2191-2198.  
  7588629 C.H.Lee, B.Leung, M.A.Lemmon, J.Zheng, D.Cowburn, J.Kuriyan, and K.Saksela (1995).
A single amino acid in the SH3 domain of Hck determines its high affinity and specificity in binding to HIV-1 Nef protein.
  EMBO J, 14, 5006-5015.  
7559773 C.M.Field, and B.M.Alberts (1995).
Anillin, a contractile ring protein that cycles from the nucleus to the cell cortex.
  J Cell Biol, 131, 165-178.  
7797522 C.Schumacher, B.S.Knudsen, T.Ohuchi, P.P.Di Fiore, R.H.Glassman, and H.Hanafusa (1995).
The SH3 domain of Crk binds specifically to a conserved proline-rich motif in Eps15 and Eps15R.
  J Biol Chem, 270, 15341-15347.  
  7588608 D.N.Sgouras, M.A.Athanasiou, G.J.Beal, R.J.Fisher, D.G.Blair, and G.J.Mavrothalassitis (1995).
ERF: an ETS domain protein with strong transcriptional repressor activity, can suppress ets-associated tumorigenesis and is regulated by phosphorylation during cell cycle and mitogenic stimulation.
  EMBO J, 14, 4781-4793.  
7834743 G.B.Cohen, R.Ren, and D.Baltimore (1995).
Modular binding domains in signal transduction proteins.
  Cell, 80, 237-248.  
7629518 G.O.Cory, R.C.Lovering, S.Hinshelwood, L.MacCarthy-Morrogh, R.J.Levinsky, and C.Kinnon (1995).
The protein product of the c-cbl protooncogene is phosphorylated after B cell receptor stimulation and binds the SH3 domain of Bruton's tyrosine kinase.
  J Exp Med, 182, 611-615.  
7540055 G.Siligardi, and A.F.Drake (1995).
The importance of extended conformations and, in particular, the PII conformation for the molecular recognition of peptides.
  Biopolymers, 37, 281-292.  
7592945 I.Van Seuningen, J.Ostrowski, X.R.Bustelo, P.R.Sleath, and K.Bomsztyk (1995).
The K protein domain that recruits the interleukin 1-responsive K protein kinase lies adjacent to a cluster of c-Src and Vav SH3-binding sites. Implications that K protein acts as a docking platform.
  J Biol Chem, 270, 26976-26985.  
9383403 J.A.Simon, and S.L.Schreiber (1995).
Grb2 SH3 binding to peptides from Sos: evaluation of a general model for SH3-ligand interactions.
  Chem Biol, 2, 53-60.  
7553130 J.M.Robinson, and J.A.Badwey (1995).
The NADPH oxidase complex of phagocytic leukocytes: a biochemical and cytochemical view.
  Histochem Cell Biol, 103, 163-180.  
  8535233 J.W.Becker, A.I.Marcy, L.L.Rokosz, M.G.Axel, J.J.Burbaum, P.M.Fitzgerald, P.M.Cameron, C.K.Esser, W.K.Hagmann, and J.D.Hermes (1995).
Stromelysin-1: three-dimensional structure of the inhibited catalytic domain and of the C-truncated proenzyme.
  Protein Sci, 4, 1966-1976.
PDB codes: 1slm 1sln
7536925 K.Alexandropoulos, G.Cheng, and D.Baltimore (1995).
Proline-rich sequences that bind to Src homology 3 domains with individual specificities.
  Proc Natl Acad Sci U S A, 92, 3110-3114.  
9383454 K.M.Shokat (1995).
Tyrosine kinases: modular signaling enzymes with tunable specificities.
  Chem Biol, 2, 509-514.  
7592689 P.Xu, A.S.Zot, and H.G.Zot (1995).
Identification of Acan125 as a myosin-I-binding protein present with myosin-I on cellular organelles of Acanthamoeba.
  J Biol Chem, 270, 25316-25319.  
7479908 R.J.Rickles, M.C.Botfield, X.M.Zhou, P.A.Henry, J.S.Brugge, and M.J.Zoller (1995).
Phage display selection of ligand residues important for Src homology 3 domain binding specificity.
  Proc Natl Acad Sci U S A, 92, 10909-10913.  
8618911 S.Feng, C.Kasahara, R.J.Rickles, and S.L.Schreiber (1995).
Specific interactions outside the proline-rich core of two classes of Src homology 3 ligands.
  Proc Natl Acad Sci U S A, 92, 12408-12415.
PDB codes: 1qwe 1qwf
7629138 S.S.Yang, L.Van Aelst, and D.Bar-Sagi (1995).
Differential interactions of human Sos1 and Sos2 with Grb2.
  J Biol Chem, 270, 18212-18215.  
7642581 T.Fukazawa, K.A.Reedquist, T.Trub, S.Soltoff, G.Panchamoorthy, B.Druker, L.Cantley, S.E.Shoelson, and H.Band (1995).
The SH3 domain-binding T cell tyrosyl phosphoprotein p120. Demonstration of its identity with the c-cbl protooncogene product and in vivo complexes with Fyn, Grb2, and phosphatidylinositol 3-kinase.
  J Biol Chem, 270, 19141-19150.  
  7781603 T.Raabe, J.P.Olivier, B.Dickson, X.Liu, G.D.Gish, T.Pawson, and E.Hafen (1995).
Biochemical and genetic analysis of the Drk SH2/SH3 adaptor protein of Drosophila.
  EMBO J, 14, 2509-2518.  
7657668 W.Yang, S.N.Malek, and S.Desiderio (1995).
An SH3-binding site conserved in Bruton's tyrosine kinase and related tyrosine kinases mediates specific protein interactions in vitro and in vivo.
  J Biol Chem, 270, 20832-20840.  
7735837 X.Wu, B.Knudsen, S.M.Feller, J.Zheng, A.Sali, D.Cowburn, H.Hanafusa, and J.Kuriyan (1995).
Structural basis for the specific interaction of lysine-containing proline-rich peptides with the N-terminal SH3 domain of c-Crk.
  Structure, 3, 215-226.
PDB codes: 1cka 1ckb
7775479 X.X.Xu, W.Yang, S.Jackowski, and C.O.Rock (1995).
Cloning of a novel phosphoprotein regulated by colony-stimulating factor 1 shares a domain with the Drosophila disabled gene product.
  J Biol Chem, 270, 14184-14191.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.