spacer
spacer

PDBsum entry 1se3

Go to PDB code: 
protein ligands links
Toxin PDB id
1se3
Jmol
Contents
Protein chain
239 a.a. *
Ligands
SIA-GAL-BGC
Waters ×135
* Residue conservation analysis
PDB id:
1se3
Name: Toxin
Title: Staphylococcal enterotoxin b complexed with gm3 trisaccharide
Structure: Staphylococcal enterotoxin b. Chain: a. Synonym: seb. Engineered: yes. Other_details: complexed with gm3 trisaccharide (3'- sialyllactose)
Source: Staphylococcus aureus. Organism_taxid: 1280. Cell_line: s2. Expressed in: staphylococcus aureus. Expression_system_taxid: 1280
Resolution:
2.30Å     R-factor:   0.220     R-free:   0.330
Authors: S.Swaminathan,M.Sax
Key ref: S.Swaminathan et al. (1995). Residues defining V beta specificity in staphylococcal enterotoxins. Nat Struct Biol, 2, 680-686. PubMed id: 7552730
Date:
11-Oct-96     Release date:   16-Jun-97    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P01552  (ETXB_STAAU) -  Enterotoxin type B
Seq:
Struc:
266 a.a.
239 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     extracellular region   1 term 
  Biological process     pathogenesis   1 term 

 

 
Nat Struct Biol 2:680-686 (1995)
PubMed id: 7552730  
 
 
Residues defining V beta specificity in staphylococcal enterotoxins.
S.Swaminathan, W.Furey, J.Pletcher, M.Sax.
 
  ABSTRACT  
 
The three-dimensional structure of staphylococcal enterotoxin C2 has been determined at 2.7 A resolution by x-ray diffraction, while the structures of enterotoxins A and E have been modelled based on their sequence homology to other staphylococcal enterotoxins. The T-cell receptor-binding sites of staphylococcal enterotoxin (SE) B and SEC2 are compared and the stereochemical interactions likely to be responsible for their differing V beta specificities are identified. A similar comparison is made between SEA and SEE.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
20554806 T.K.Inskeep, C.Stahl, J.Odle, J.Oakes, L.Hudson, K.L.Bost, and K.J.Piller (2010).
Oral vaccine formulations stimulate mucosal and systemic antibody responses against staphylococcal enterotoxin B in a piglet model.
  Clin Vaccine Immunol, 17, 1163-1169.  
19593815 J.A.Garnett, Y.Liu, E.Leon, S.A.Allman, N.Friedrich, S.Saouros, S.Curry, D.Soldati-Favre, B.G.Davis, T.Feizi, and S.Matthews (2009).
Detailed insights from microarray and crystallographic studies into carbohydrate recognition by microneme protein 1 (MIC1) of Toxoplasma gondii.
  Protein Sci, 18, 1935-1947.
PDB codes: 3f53 3f5a 3f5e
18809496 A.Imberty, and A.Varrot (2008).
Microbial recognition of human cell surface glycoconjugates.
  Curr Opin Struct Biol, 18, 567-576.  
18506495 J.Hui, Y.Cao, F.Xiao, J.Zhang, H.Li, and F.Hu (2008).
Staphylococcus aureus enterotoxin C2 mutants: biological activity assay in vitro.
  J Ind Microbiol Biotechnol, 35, 975-980.  
14559915 A.C.Papageorgiou, M.D.Baker, J.D.McLeod, S.K.Goda, C.N.Manzotti, D.M.Sansom, H.S.Tranter, and K.R.Acharya (2004).
Identification of a secondary zinc-binding site in staphylococcal enterotoxin C2. Implications for superantigen recognition.
  J Biol Chem, 279, 1297-1303.
PDB code: 1uns
11895985 J.W.Shupp, M.Jett, and C.H.Pontzer (2002).
Identification of a transcytosis epitope on staphylococcal enterotoxins.
  Infect Immun, 70, 2178-2186.  
11934896 Y.I.Chi, I.Sadler, L.M.Jablonski, S.D.Callantine, C.F.Deobald, C.V.Stauffacher, and G.A.Bohach (2002).
Zinc-mediated dimerization and its effect on activity and conformation of staphylococcal enterotoxin type C.
  J Biol Chem, 277, 22839-22846.
PDB code: 1ck1
11290341 C.T.Morita, H.Li, J.G.Lamphear, R.R.Rich, J.D.Fraser, R.A.Mariuzza, and H.K.Lee (2001).
Superantigen recognition by gammadelta T cells: SEA recognition site for human Vgamma2 T cell receptors.
  Immunity, 14, 331-344.  
9878045 A.C.Papageorgiou, C.M.Collins, D.M.Gutman, J.B.Kline, S.M.O'Brien, H.S.Tranter, and K.R.Acharya (1999).
Structural basis for the recognition of superantigen streptococcal pyrogenic exotoxin A (SpeA1) by MHC class II molecules and T-cell receptors.
  EMBO J, 18, 9.
PDB code: 1b1z
10399079 P.M.Lavoie, J.Thibodeau, F.Erard, and R.P.Sékaly (1999).
Understanding the mechanism of action of bacterial superantigens from a decade of research.
  Immunol Rev, 168, 257-269.  
10406939 Y.Ito, G.Seprényi, J.Abe, and T.Kohsaka (1999).
Analysis of functional regions of YPM, a superantigen derived from gram-negative bacteria.
  Eur J Biochem, 263, 326-337.  
9585531 C.A.Earhart, D.T.Mitchell, D.L.Murray, D.M.Pinheiro, M.Matsumura, P.M.Schlievert, and D.H.Ohlendorf (1998).
Structures of five mutants of toxic shock syndrome toxin-1 with reduced biological activity.
  Biochemistry, 37, 7194-7202.
PDB codes: 1aw7 1ts2 1ts3 1ts4 1ts5
9309216 A.C.Papageorgiou, and K.R.Acharya (1997).
Superantigens as immunomodulators: recent structural insights.
  Structure, 5, 991-996.  
9253413 A.Roussel, B.F.Anderson, H.M.Baker, J.D.Fraser, and E.N.Baker (1997).
Crystal structure of the streptococcal superantigen SPE-C: dimerization and zinc binding suggest a novel mode of interaction with MHC class II molecules.
  Nat Struct Biol, 4, 635-643.
PDB code: 1an8
  9194182 G.S.Prasad, R.Radhakrishnan, D.T.Mitchell, C.A.Earhart, M.M.Dinges, W.J.Cook, P.M.Schlievert, and D.H.Ohlendorf (1997).
Refined structures of three crystal forms of toxic shock syndrome toxin-1 and of a tetramutant with reduced activity.
  Protein Sci, 6, 1220-1227.
PDB codes: 2tss 3tss 4tss 5tss
8879218 L.Liao, A.Marinescu, A.Molano, C.Ciurli, R.P.Sekaly, J.D.Fraser, A.Popowicz, and D.N.Posnett (1996).
TCR binding differs for a bacterial superantigen (SEE) and a viral superantigen (Mtv-9).
  J Exp Med, 184, 1471-1482.  
  8757827 R.J.Neill, M.Jett, R.Crane, J.Wootres, C.Welch, D.Hoover, and P.Gemski (1996).
Mitogenic activities of amino acid substitution mutants of staphylococcal enterotoxin B in human and mouse lymphocyte cultures.
  Infect Immun, 64, 3007-3015.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.