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* Residue conservation analysis
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Gene Ontology (GO) functional annotation
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Biochemical function
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sugar binding
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1 term
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DOI no:
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Microbiology
149:3361-3370
(2003)
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PubMed id:
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Structural analysis by X-ray crystallography and calorimetry of a haemagglutinin component (HA1) of the progenitor toxin from Clostridium botulinum.
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K.Inoue,
M.Sobhany,
T.R.Transue,
K.Oguma,
L.C.Pedersen,
M.Negishi.
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ABSTRACT
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Botulism food poisoning is caused primarily by ingestion of the Clostridium
botulinum neurotoxin (BoNT). The 1300 amino acid BoNT forms a progenitor toxin
(PTX) that, when associated with a number of other proteins, increases its oral
toxicity by protecting it from the low pH of the stomach and from intestinal
proteases. One of these associated proteins, HA1, has also been suggested to be
involved with internalization of the toxin into the bloodstream by binding to
oligosaccharides lining the intestine. Here is reported the crystal structure of
HA1 from type C Clostridium botulinum at a resolution of 1.7 Angstrom. The
protein consists of two beta-trefoil domains and bears structural similarities
to the lectin B-chain from the deadly plant toxin ricin. Based on structural
comparison to the ricin B-chain lactose-binding sites, residues of type A HA1
were selected and mutated. The D263A and N285A mutants lost the ability to bind
carbohydrates containing galactose moieties, implicating these residues in
carbohydrate binding.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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N.J.Mantis,
and
S.J.Forbes
(2010).
Secretory IgA: arresting microbial pathogens at epithelial borders.
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Immunol Invest, 39,
383-406.
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H.Hemmi,
A.Kuno,
S.Ito,
R.Suzuki,
T.Hasegawa,
and
J.Hirabayashi
(2009).
NMR studies on the interaction of sugars with the C-terminal domain of an R-type lectin from the earthworm Lumbricus terrestris.
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FEBS J, 276,
2095-2105.
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R.J.Mrsny
(2009).
Lessons from nature: "Pathogen-Mimetic" systems for mucosal nano-medicines.
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Adv Drug Deliv Rev, 61,
172-192.
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T.Nakamura,
T.Tonozuka,
M.Kotani,
K.Obata,
K.Oguma,
and
A.Nishikawa
(2007).
Crystallization and preliminary X-ray analysis of the HA3 component of Clostridium botulinum type C progenitor toxin.
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Acta Crystallogr Sect F Struct Biol Cryst Commun, 63,
1038-1040.
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I.Carpusca,
T.Jank,
and
K.Aktories
(2006).
Bacillus sphaericus mosquitocidal toxin (MTX) and pierisin: the enigmatic offspring from the family of ADP-ribosyltransferases.
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Mol Microbiol, 62,
621-630.
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W.K.Kim,
A.Henschel,
C.Winter,
and
M.Schroeder
(2006).
The many faces of protein-protein interactions: A compendium of interface geometry.
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PLoS Comput Biol, 2,
e124.
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A.Imberty,
E.P.Mitchell,
and
M.Wimmerová
(2005).
Structural basis of high-affinity glycan recognition by bacterial and fungal lectins.
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Curr Opin Struct Biol, 15,
525-534.
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
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Links
