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PDBsum entry 1qrh

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protein dna_rna links
Hydrolase/DNA PDB id
1qrh
Jmol
Contents
Protein chain
261 a.a.
DNA/RNA
Waters ×51
PDB id:
1qrh
Name: Hydrolase/DNA
Title: X-ray structure of the DNA-eco ri endonuclease complexes with an r145k mutation at 2.7 a
Structure: 5'-(tp Cp Gp Cp Gp Ap Ap Tp Tp Cp Gp Cp G )-3'. Chain: m. Engineered: yes. Eco ri endonculease. Chain: a. Fragment: residues 17-277. Mutation: yes
Source: Synthetic: yes. Escherichia coli. Organism_taxid: 562
Biol. unit: Tetramer (from PDB file)
Resolution:
2.50Å     R-factor:   0.161    
Authors: J.Choi,Y.Kim,P.Greene,P.Hager,J.M.Rosenberg
Key ref: J.Choi et al. X-Ray structure of the DNA-Eco ri endonuclease complexes with the ed144 and rk145 mutations. To be published, .
Date:
14-Jun-99     Release date:   23-Jun-99    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
P00642  (T2E1_ECOLX) -  Type-2 restriction enzyme EcoRI
Seq:
Struc:
277 a.a.
261 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Enzyme reactions 
   Enzyme class: E.C.3.1.21.4  - Type Ii site-specific deoxyribonuclease.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.
      Cofactor: Mg(2+)
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     nucleic acid phosphodiester bond hydrolysis   3 terms 
  Biochemical function     hydrolase activity     7 terms