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PDBsum entry 1qps

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protein dna_rna metals links
Hydrolase/DNA PDB id
1qps
Jmol
Contents
Protein chain
261 a.a.
DNA/RNA
Metals
_MN
Waters ×44
PDB id:
1qps
Name: Hydrolase/DNA
Title: The crystal structure of a post-reactive cognate DNA-eco ri complex at 2.50 a in the presence of mn2+ ion
Structure: 5'-d( Tp Cp Gp Cp Gp )-3'. Chain: m. Engineered: yes. 5'-d( Ap Ap Tp Tp Cp Gp Cp Gp )-3'. Chain: n. Engineered: yes. Endonuclease ecori. Chain: a. Fragment: residues 17-277.
Source: Synthetic: yes. Other_details: recognition sequence. Escherichia coli. Organism_taxid: 562
Resolution:
2.50Å     R-factor:   not given    
Authors: M.Horvath,J.Choi,Y.Kim,P.Wilkosz,J.M.Rosenberg
Key ref: M.M.Horvath et al. The integration of recognition and cleavage: X-Ray structures of pre- Transition state and post-Reactive DNA-Eco ri endonuclease complexes. To be published, .
Date:
28-May-99     Release date:   14-Jun-99    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
P00642  (T2E1_ECOLX) -  Type-2 restriction enzyme EcoRI
Seq:
Struc:
277 a.a.
261 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: E.C.3.1.21.4  - Type Ii site-specific deoxyribonuclease.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.
      Cofactor: Mg(2+)
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     nucleic acid phosphodiester bond hydrolysis   3 terms 
  Biochemical function     hydrolase activity     7 terms