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43 a.a.
Key reference
DOI no: 10.1016/j.bbrc.2003.12.068 Biochem Biophys Res Commun 314:123-130 (2004) PubMed id: 14715255
Insights into a conformational epitope of Hev b 6.02 (hevein). C.A.Reyes-López, A.Hernández-Santoyo, M.Pedraza-Escalona, G.Mendoza, A.Hernández-Arana, A.Rodríguez-Romero. ABSTRACT
Hevein (Hev b 6.02) is a major IgE-binding allergen in natural rubber latex and manufactured products. Both tryptophans (Trp(21) and Trp(23)) of the hevein molecule were chemically modified with BNPS-skatole (2-nitrophenylsulfenyl-3-methyl-3(')-bromoindolenine); derivatized allergen failed to significantly inhibit binding of serum IgE in ELISA assays. Similarly, skin prick tests showed that hevein-positive patients gave no response with the modified allergen. Dot blot experiments carried out with anti-hevein mono- and polyclonal antibodies confirmed the importance of Trp(21) and Trp(23) for antibody-recognition, and demonstrated the specific cross-reactivity of other molecules containing hevein-like domains. We also report the structure of Hev b 6.02 at an extended resolution (1.5A) and compare its surface properties around Trp residues with those of similar regions in other allergens. Overall our results indicate that the central part of the protein, which comprises three aromatic and other acidic and polar residues, constitutes a conformational epitope.
Literature references that cite this PDB file's key reference
PubMed id Reference
18331637 K.Manikandan, D.Pal, S.Ramakumar, N.E.Brener, S.S.Iyengar, and G.Seetharaman (2008).
Functionally important segments in proteins dissected using Gene Ontology and geometric clustering of peptide fragments.Genome Biol, 9, R52. 17768356 D.Fuentes-Silva, G.Mendoza-Hernández, V.Stojanoff, L.A.Palomares, E.Zenteno, A.Torres-Larios, and A.Rodríguez-Romero (2007).
Crystallization and identification of the glycosylated moieties of two isoforms of the main allergen Hev b 2 and preliminary X-ray analysis of two polymorphs of isoform II.Acta Crystallogr Sect F Struct Biol Cryst Commun, 63, 787-791.
17724453 M.D.Cunningham (2007).
Neonatal latex allergy.J Perinatol, 27, 533-534.
16264336 J.M.Rolland, A.C.Drew, and R.E.O'Hehir (2005).
Advances in development of hypoallergenic latex immunotherapy.Curr Opin Allergy Clin Immunol, 5, 544-551.
16220560 M.I.Chávez, C.Andreu, P.Vidal, N.Aboitiz, F.Freire, P.Groves, J.L.Asensio, G.Asensio, M.Muraki, F.J.Cañada, and J.Jiménez-Barbero (2005).
On the importance of carbohydrate-aromatic interactions for the molecular recognition of oligosaccharides by proteins: NMR studies of the structure and binding affinity of AcAMP2-like peptides with non-natural naphthyl and fluoroaromatic residues.Chemistry, 11, 7060-7074.
PDB codes: 1znt 1zuv 1zwu
16335802 N.Kamal, S.Chowdhury, T.Madan, D.Sharma, M.Attreyi, W.Haq, S.B.Katti, A.Kumar, and P.U.Sarma (2005).
Tryptophan residue is essential for immunoreactivity of a diagnostically relevant peptide epitope of A. fumigatus.Mol Cell Biochem, 275, 223-231. The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.
