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* Residue conservation analysis
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Gene Ontology (GO) functional annotation
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Biological process
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cell wall macromolecule catabolic process
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2 terms
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Biochemical function
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chitin binding
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2 terms
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DOI no:
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Biochem Biophys Res Commun
314:123-130
(2004)
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PubMed id:
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Insights into a conformational epitope of Hev b 6.02 (hevein).
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C.A.Reyes-López,
A.Hernández-Santoyo,
M.Pedraza-Escalona,
G.Mendoza,
A.Hernández-Arana,
A.Rodríguez-Romero.
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ABSTRACT
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Hevein (Hev b 6.02) is a major IgE-binding allergen in natural rubber latex and
manufactured products. Both tryptophans (Trp(21) and Trp(23)) of the hevein
molecule were chemically modified with BNPS-skatole
(2-nitrophenylsulfenyl-3-methyl-3(')-bromoindolenine); derivatized allergen
failed to significantly inhibit binding of serum IgE in ELISA assays. Similarly,
skin prick tests showed that hevein-positive patients gave no response with the
modified allergen. Dot blot experiments carried out with anti-hevein mono- and
polyclonal antibodies confirmed the importance of Trp(21) and Trp(23) for
antibody-recognition, and demonstrated the specific cross-reactivity of other
molecules containing hevein-like domains. We also report the structure of Hev b
6.02 at an extended resolution (1.5A) and compare its surface properties around
Trp residues with those of similar regions in other allergens. Overall our
results indicate that the central part of the protein, which comprises three
aromatic and other acidic and polar residues, constitutes a conformational
epitope.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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J.J.Hernández-Gay,
A.Ardá,
S.Eller,
S.Mezzato,
B.R.Leeflang,
C.Unverzagt,
F.J.Cañada,
and
J.Jiménez-Barbero
(2010).
Insights into the dynamics and molecular recognition features of glycopeptides by protein receptors: the 3D solution structure of hevein bound to the trisaccharide core of N-glycoproteins.
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Chemistry, 16,
10715-10726.
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Y.Kezuka,
M.Kojima,
R.Mizuno,
K.Suzuki,
T.Watanabe,
and
T.Nonaka
(2010).
Structure of full-length class I chitinase from rice revealed by X-ray crystallography and small-angle X-ray scattering.
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Proteins, 78,
2295-2305.
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PDB code:
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K.Manikandan,
D.Pal,
S.Ramakumar,
N.E.Brener,
S.S.Iyengar,
and
G.Seetharaman
(2008).
Functionally important segments in proteins dissected using Gene Ontology and geometric clustering of peptide fragments.
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Genome Biol, 9,
R52.
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D.Fuentes-Silva,
G.Mendoza-Hernández,
V.Stojanoff,
L.A.Palomares,
E.Zenteno,
A.Torres-Larios,
and
A.Rodríguez-Romero
(2007).
Crystallization and identification of the glycosylated moieties of two isoforms of the main allergen Hev b 2 and preliminary X-ray analysis of two polymorphs of isoform II.
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Acta Crystallogr Sect F Struct Biol Cryst Commun, 63,
787-791.
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M.D.Cunningham
(2007).
Neonatal latex allergy.
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J Perinatol, 27,
533-534.
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J.M.Rolland,
A.C.Drew,
and
R.E.O'Hehir
(2005).
Advances in development of hypoallergenic latex immunotherapy.
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Curr Opin Allergy Clin Immunol, 5,
544-551.
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M.I.Chávez,
C.Andreu,
P.Vidal,
N.Aboitiz,
F.Freire,
P.Groves,
J.L.Asensio,
G.Asensio,
M.Muraki,
F.J.Cañada,
and
J.Jiménez-Barbero
(2005).
On the importance of carbohydrate-aromatic interactions for the molecular recognition of oligosaccharides by proteins: NMR studies of the structure and binding affinity of AcAMP2-like peptides with non-natural naphthyl and fluoroaromatic residues.
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Chemistry, 11,
7060-7074.
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PDB codes:
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N.Kamal,
S.Chowdhury,
T.Madan,
D.Sharma,
M.Attreyi,
W.Haq,
S.B.Katti,
A.Kumar,
and
P.U.Sarma
(2005).
Tryptophan residue is essential for immunoreactivity of a diagnostically relevant peptide epitope of A. fumigatus.
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Mol Cell Biochem, 275,
223-231.
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N.Aboitiz,
M.Vila-Perelló,
P.Groves,
J.L.Asensio,
D.Andreu,
F.J.Cañada,
and
J.Jiménez-Barbero
(2004).
NMR and modeling studies of protein-carbohydrate interactions: synthesis, three-dimensional structure, and recognition properties of a minimum hevein domain with binding affinity for chitooligosaccharides.
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Chembiochem, 5,
1245-1255.
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PDB code:
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
code is
shown on the right.
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