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PDBsum entry 1q7f
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* Residue conservation analysis
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Genes Dev
17:2508-2513
(2003)
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PubMed id:
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Model of the brain tumor-Pumilio translation repressor complex.
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T.A.Edwards,
B.D.Wilkinson,
R.P.Wharton,
A.K.Aggarwal.
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ABSTRACT
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The Brain Tumor (Brat) protein is recruited to the 3' untranslated region (UTR)
of hunchback mRNA to regulate its translation. Recruitment is mediated by
interactions between the Pumilio RNA-binding Puf repeats and the NHL domain of
Brat, a conserved structural motif present in a large family of growth
regulators. In this report, we describe the crystal structure of the Brat NHL
domain and present a model of the Pumilio-Brat complex derived from in silico
docking experiments and supported by mutational analysis of the protein-protein
interface. A key feature of the model is recognition of the outer, convex
surface of the Pumilio Puf domain by the top, electropositive face of the
six-bladed Brat beta-propeller. In particular, an extended loop in Puf repeat 8
fits in the entrance to the central channel of the Brat beta-propeller.
Together, these interactions are likely to be prototypic of the recruitment
strategies of other NHL-containing proteins in development.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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A.G.Cridge,
L.M.Castelli,
J.B.Smirnova,
J.N.Selley,
W.Rowe,
S.J.Hubbard,
J.E.McCarthy,
M.P.Ashe,
C.M.Grant,
and
G.D.Pavitt
(2010).
Identifying eIF4E-binding protein translationally-controlled transcripts reveals links to mRNAs bound by specific PUF proteins.
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Nucleic Acids Res,
38,
8039-8050.
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P.P.Tam,
I.H.Barrette-Ng,
D.M.Simon,
M.W.Tam,
A.L.Ang,
and
D.G.Muench
(2010).
The Puf family of RNA-binding proteins in plants: phylogeny, structural modeling, activity and subcellular localization.
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BMC Plant Biol,
10,
44.
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A.Rybak,
H.Fuchs,
K.Hadian,
L.Smirnova,
E.A.Wulczyn,
G.Michel,
R.Nitsch,
D.Krappmann,
and
F.G.Wulczyn
(2009).
The let-7 target gene mouse lin-41 is a stem cell specific E3 ubiquitin ligase for the miRNA pathway protein Ago2.
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Nat Cell Biol,
11,
1411-1420.
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M.Locke,
C.L.Tinsley,
M.A.Benson,
and
D.J.Blake
(2009).
TRIM32 is an E3 ubiquitin ligase for dysbindin.
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Hum Mol Genet,
18,
2344-2358.
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M.S.Gentry,
J.E.Dixon,
and
C.A.Worby
(2009).
Lafora disease: insights into neurodegeneration from plant metabolism.
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Trends Biochem Sci,
34,
628-639.
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C.A.Worby,
M.S.Gentry,
and
J.E.Dixon
(2008).
Malin decreases glycogen accumulation by promoting the degradation of protein targeting to glycogen (PTG).
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J Biol Chem,
283,
4069-4076.
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F.O'Farrell,
S.S.Esfahani,
Y.Engström,
and
P.Kylsten
(2008).
Regulation of the Drosophila lin-41 homologue dappled by let-7 reveals conservation of a regulatory mechanism within the LIN-41 subclade.
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Dev Dyn,
237,
196-208.
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I.Chaudhuri,
J.Söding,
and
A.N.Lupas
(2008).
Evolution of the beta-propeller fold.
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Proteins,
71,
795-803.
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V.Saccone,
M.Palmieri,
L.Passamano,
G.Piluso,
G.Meroni,
L.Politano,
and
V.Nigro
(2008).
Mutations that impair interaction properties of TRIM32 associated with limb-girdle muscular dystrophy 2H.
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Hum Mutat,
29,
240-247.
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J.Stöckigt,
and
S.Panjikar
(2007).
Structural biology in plant natural product biosynthesis--architecture of enzymes from monoterpenoid indole and tropane alkaloid biosynthesis.
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Nat Prod Rep,
24,
1382-1400.
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C.A.Worby,
M.S.Gentry,
and
J.E.Dixon
(2006).
Laforin, a dual specificity phosphatase that dephosphorylates complex carbohydrates.
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J Biol Chem,
281,
30412-30418.
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P.F.Cho,
C.Gamberi,
Y.A.Cho-Park,
I.B.Cho-Park,
P.Lasko,
and
N.Sonenberg
(2006).
Cap-dependent translational inhibition establishes two opposing morphogen gradients in Drosophila embryos.
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Curr Biol,
16,
2035-2041.
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E.Glasscock,
A.Singhania,
and
M.A.Tanouye
(2005).
The mei-P26 gene encodes a RING finger B-box coiled-coil-NHL protein that regulates seizure susceptibility in Drosophilia.
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Genetics,
170,
1677-1689.
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J.Urano,
M.S.Fox,
and
R.A.Reijo Pera
(2005).
Interaction of the conserved meiotic regulators, BOULE (BOL) and PUMILIO-2 (PUM2).
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Mol Reprod Dev,
71,
290-298.
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M.J.Pallen,
S.A.Beatson,
and
C.M.Bailey
(2005).
Bioinformatics analysis of the locus for enterocyte effacement provides novel insights into type-III secretion.
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BMC Microbiol,
5,
9.
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M.S.Gentry,
C.A.Worby,
and
J.E.Dixon
(2005).
Insights into Lafora disease: malin is an E3 ubiquitin ligase that ubiquitinates and promotes the degradation of laforin.
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Proc Natl Acad Sci U S A,
102,
8501-8506.
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Y.Chen,
and
G.Varani
(2005).
Protein families and RNA recognition.
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FEBS J,
272,
2088-2097.
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T.Loop,
R.Leemans,
U.Stiefel,
L.Hermida,
B.Egger,
F.Xie,
M.Primig,
U.Certa,
K.F.Fischbach,
H.Reichert,
and
F.Hirth
(2004).
Transcriptional signature of an adult brain tumor in Drosophila.
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BMC Genomics,
5,
24.
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
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