PDBsum entry: 1q6z

Lyase

PDB id: 1q6z

Contents

Protein chain

524 a.a. *

Ligands

TZD

Metals

_MG

_CA ×3

Waters ×588

* Residue conservation analysis

PDB id:

1q6z

Name:

Lyase

Title:

High resolution structure of e28a mutant benzoylformate deca from pseudomonas putida complexed with thiamin thiazolone d

Structure:

Benzoylformate decarboxylase. Chain: a. Synonym: bfd, bfdc. Engineered: yes. Mutation: yes

Source:

Pseudomonas putida. Organism_taxid: 303. Strain: rf4738. Atcc: 12633. Gene: mdlc. Expressed in: escherichia coli. Expression_system_taxid: 562.

Biol. unit:

Tetramer (from PDB file)

Resolution:

1.00Å R-factor: 0.122 R-free: 0.140

Authors:

A.K.Bera,M.S.Hasson

Key ref:

A.K.Bera et al. High resolution structure of e28a mutant benzoylformate decarboxylase from pseudomonas putida complexed with thiamin thiazolone diphosphate. To be published,

Date:

14-Aug-03 Release date: 23-Nov-04

Protein chain

P20906  (MDLC_PSEPU) -  Benzoylformate decarboxylase

Seq: 528 a.a.

Struc: 524 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.4.1.1.7 - Benzoylformate decarboxylase.
• Reaction: Benzoylformate = benzaldehyde + CO₂
• Cofactor: Thiamine diphosphate

Thiamine diphosphate (Bound ligand (Het Group name = TZD) matches with 96.30% similarity)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

 Gene Ontology (GO) functional annotation 

• Biological process: aromatic compound catabolic process (2 terms)
• Biochemical function: catalytic activity (8 terms)