 |
|
|
|
|
|
 |
Contents |
 |
|
|
|
|
|
|
|
|
|
|
* Residue conservation analysis
|
|
|
|
|
|
PDB id:
|
|
|
|
| Name: |
|
Ligase
|
|
|
Title:
|
|
Crystal structures of two ubc (e2) enzymes of the ubiquitin- conjugating system in caenorhabditis elegans
|
|
Structure:
|
|
Ubiquitin-conjugating enzyme e2-21.5 kda. Chain: a, b, c. Synonym: ubiquitin- protein ligase, ubiquitin carrier protein. Engineered: yes
|
|
Source:
|
|
Caenorhabditis elegans. Organism_taxid: 6239. Gene: ubc-1 or c35b1.1. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
|
|
Resolution:
|
|
|
2.90Å
|
R-factor:
|
0.233
|
R-free:
|
0.266
|
|
|
Authors:
|
|
N.Schormann,G.Lin,S.Li,J.Symersky,S.Qiu,J.Finley,D.Luo, A.Stanton,M.Carson,M.Luo,Southeast Collaboratory For Structural Genomics (Secsg)
|
|
Key ref:
|
|
N.Schormann
et al.
Crystal structures of two ubc (e2) enzymes of the ubiquitin-Conjugating system in caenorhabditis elegans.
To be published,
|
|
|
Date:
|
|
|
28-Jul-03
|
Release date:
|
05-Aug-03
|
|
|
|
|
|
|
PROCHECK
|
|
|
|
|
|
Headers
|
|
|
|
References
|
|
|
|
|
|
|
|
P52478
(UBC1_CAEEL) -
Ubiquitin-conjugating enzyme E2 1
|
|
|
|
Seq:
Struc:
|
|
|
|
192 a.a.
149 a.a.
|
|
|
|
|
|
|
|
|
|
|
|
|
Key: |
 |
PfamA domain |
|
|
 |
Secondary structure |
|
 |
CATH domain |
|
|
|
|
|
|
|
|
|
|
|
|
|
Enzyme class:
|
|
E.C.6.3.2.19
- Ubiquitin--protein ligase.
|
|
|
|
|
|
|
Reaction:
|
|
ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine
|
|
|
|
|
|
ATP
|
+
|
ubiquitin
|
+
|
protein lysine
|
=
|
AMP
|
+
|
diphosphate
|
+
|
protein N-ubiquityllysine
|
|
|
|
|
|
|
|
|
|
|
|
|
Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
|
|
|
|
|
|
|
|
|
|
|
Gene Ontology (GO) functional annotation
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Biological process
|
regulation of protein metabolic process
|
2 terms
|
|
|
Biochemical function
|
small conjugating protein ligase activity
|
1 term
|
|
|
|
|
|
|
|
|
|
|
Links
