PDBsum entry: 1q34

Ligase

PDB-id: 1q34

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Protein chains

149 a.a. *

Waters ×61

* Residue conservation analysis

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Clefts Calculation

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PDB id:

1q34

Name:

Ligase

Title:

Crystal structures of two ubc (e2) enzymes of the ubiquitin- conjugating system in caenorhabditis elegans

Structure:

Ubiquitin-conjugating enzyme e2-21.5 kda. Chain: a, b, c. Synonym: ubiquitin- protein ligase, ubiquitin carrier protein. Engineered: yes

Source:

Caenorhabditis elegans. Organism_taxid: 6239. Gene: ubc-1 or c35b1.1. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

UniProt:

Chains A, B, C: P52478 (UBC1_CAEEL)

Seq:

192 a.a.

Struc:

149 a.a.

Key:	PfamA domain
Secondary structure	CATH domain

Enzyme class:

E.C.6.3.2.19   [IntEnz]   [ExPASy]   [KEGG]   [BRENDA]

Reaction:

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine (see diagram below)

Resolution:

2.90Å

R-factor:

0.233

R-free:

0.266

Authors:

N.Schormann,G.Lin,S.Li,J.Symersky,S.Qiu,J.Finley,D.Luo, A.Stanton,M.Carson,M.Luo,Southeast Collaboratory For Structural Genomics (Secsg)

Key ref:

n.schormann et al. crystal structures of two ubc (e2) enzymes of the ubiquitin-conjugating system in caenorhabditis elegans. TO BE PUBLISHED, .

Date:

28-Jul-03

Release date:

05-Aug-03

Related entries:

1pzv
a similar protein (ubc-7) from c. Elegans
c35b1.1 related db: targetdb

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