PDBsum entry: 1pzw

Transcription

PDB id: 1pzw

Contents

Protein chain

80 a.a. *

Metals

_ZN

Waters ×56

* Residue conservation analysis

PDB id:

1pzw

Name:

Transcription

Title:

Crystal structure of the zinc finger associated domain of th drosophila transcription factor grauzone

Structure:

Transcription factor grauzone. Chain: a. Fragment: zinc finger associated domain (zad). Synonym: zinc finger associated domain of drosophila grauzo grauzone cg33133-pa. Engineered: yes

Source:

Drosophila melanogaster. Fruit fly. Organism_taxid: 7227. Gene: grauzone. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Biol. unit:

Dimer (from PDB file)

Resolution:

2.00Å R-factor: 0.242 R-free: 0.267

Authors:

R.Jauch,G.P.Bourenkov,H.-R.Chung,H.Urlaub,U.Reidt,H.Jaeckle,

Key ref:

R.Jauch et al. (2003). The zinc finger-associated domain of the Drosophila transcription factor grauzone is a novel zinc-coordinating protein-protein interaction module. Structure, 11, 1393-1402. PubMed id: 14604529 DOI: 10.1016/j.str.2003.09.015

Date:

14-Jul-03 Release date: 04-Nov-03

Protein chain

Q9U405  (GRAU_DROME) -  Transcription factor grauzone

Seq: 570 a.a.

Struc: 80 a.a.

 Gene Ontology (GO) functional annotation 

• Cellular component: nucleus (1 term)
• Biochemical function: zinc ion binding (1 term)

DOI no: 10.1016/j.str.2003.09.015

Structure 11:1393-1402 (2003)

PubMed id: 14604529

The zinc finger-associated domain of the Drosophila transcription factor grauzone is a novel zinc-coordinating protein-protein interaction module.

R.Jauch, G.P.Bourenkov, H.R.Chung, H.Urlaub, U.Reidt, H.Jäckle, M.C.Wahl.

ABSTRACT

About one-third of the more than 300 C2H2 zinc finger proteins of Drosophila contain a conserved sequence motif, the zinc finger-associated domain (ZAD). Genes that encode ZAD proteins are specific for and expanded in the genomes of insects. Only three ZAD-encoding gene functions are established, and the role of ZAD is unknown. Here we present the crystal structure of the ZAD of Grauzone (ZAD(Grau)), a Drosophila transcription factor that specifically controls the maternal Cdc20-like APC subunit Cortex. ZAD forms an atypical treble-clef-like zinc-coordinating fold. Head-to-tail arrangement of two ZAD(Grau) molecules in the crystals suggests dimer formation, an observation supported by crosslinking and dynamic light scattering. The results indicate that ZAD provides a novel protein-protein interaction module that characterizes a large family of insect transcription factors.

Selected figure(s)

Figure 4.
Figure 4. The ZAD[Grau] Dimer(A) Stereo ribbon plot of a ZAD[Grau] dimer as seen in the crystal. The two subunits (Mol I and Mol II) are colored red and blue; the two zinc centers are depicted in ball-and-stick. N- and C termini are labeled. The orientation of the rear subunit is the same as in Figure 2A.(B) Stereo representation of residue interactions in the dimer interface, 90° from the view in (A). The two ZAD[Grau] subunits are colored as in (A) but are rendered semitransparent to clearly reveal the interacting residues in ball-and-stick. All residues and the molecular termini are labeled.(C) Mapping of the contact residues within the dimer (green) onto the surface of a ZAD[Grau] monomer (gray). The orientation of the molecule is the same as that of the red subunit in (A).(D) Mapping in orange of conserved residues on the surface of ZAD[Grau] in two diametric views. The left orientation is the same as in (C), indicating that the largest conserved surface patch and the dimer interface largely coincide.

The above figure is reprinted by permission from Cell Press: Structure (2003, 11, 1393-1402) copyright 2003.

Figure was selected by an automated process.