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Transcription/DNA
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PDB id
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1pue
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Contents |
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* Residue conservation analysis
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PDB id:
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| Name: |
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Transcription/DNA
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Title:
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Pu.1 ets domain-DNA complex
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Structure:
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DNA (5'- d( Ap Ap Ap Ap Ap Gp Gp Gp Gp Ap Ap Gp Tp Gp Gp G)-3'). Chain: a, c. Engineered: yes. DNA (5'- d( Tp Cp Cp Cp Ap Cp Tp Tp Cp Cp Cp Cp Tp Tp Tp T)-3'). Chain: b, d. Engineered: yes. Protein (transcription factor pu.1 (tf pu.1)).
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Source:
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Synthetic: yes. Mus musculus. House mouse. Organism_taxid: 10090. Expressed in: escherichia coli. Expression_system_taxid: 562
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Biol. unit:
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Not given
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Resolution:
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2.10Å
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R-factor:
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0.225
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R-free:
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0.287
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Authors:
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R.Kodandapani,F.Pio,C.Z.Ni,G.Piccialli,M.Klemsz,S.Mckercher, R.A.Maki,K. R.Ely
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Key ref:
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R.Kodandapani
et al.
(1996).
A new pattern for helix-turn-helix recognition revealed by the PU.1 ETS-domain-DNA complex.
Nature,
380,
456-460.
PubMed id:
DOI:
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Date:
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08-Jul-96
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Release date:
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12-Feb-97
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PROCHECK
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Headers
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References
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P17433
(SPI1_MOUSE) -
Transcription factor PU.1
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Seq:
Struc:
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272 a.a.
88 a.a.*
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Key: |
 |
PfamA domain |
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Secondary structure |
|
 |
CATH domain |
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*
PDB and UniProt seqs differ
at 1 residue position (black
cross)
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Gene Ontology (GO) functional annotation
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Biological process
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regulation of transcription, DNA-dependent
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1 term
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Biochemical function
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transcription factor activity
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2 terms
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| |
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DOI no:
|
Nature
380:456-460
(1996)
|
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PubMed id:
|
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|
|
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| |
|
A new pattern for helix-turn-helix recognition revealed by the PU.1 ETS-domain-DNA complex.
|
|
R.Kodandapani,
F.Pio,
C.Z.Ni,
G.Piccialli,
M.Klemsz,
S.McKercher,
R.A.Maki,
K.R.Ely.
|
|
|
|
|
| |
ABSTRACT
|
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| |
|
|
The Ets family of transcription factors, of which there are now about 35 members
regulate gene expression during growth and development. They share a conserved
domain of around 85 amino acids which binds as a monomer to the DNA sequence
5'-C/AGGAA/T-3'. We have determined the crystal structure of an ETS domain
complexed with DNA, at 2.3-A resolution. The domain is similar to alpha + beta
(winged) 'helix-turn-helix' proteins and interacts with a ten-base-pair region
of duplex DNA which takes up a uniform curve of 8 degrees. The domain contacts
the DNA by a novel loop-helix-loop architecture. Four of amino acids that
directly interact with the DNA are highly conserved: two arginines from the
recognition helix lying in the major groove, one lysine from the 'wing' that
binds upstream of the core GGAA sequence, and another lysine, from the 'turn' of
the 'helix-turn-helix' motif, which binds downstream and on the opposite strand.
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 |
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Literature references that cite this PDB file's key reference
|
|
|
| |
PubMed id
|
|
Reference
|
|
|
|
|
|
G.H.Wei,
G.Badis,
M.F.Berger,
T.Kivioja,
K.Palin,
M.Enge,
M.Bonke,
A.Jolma,
M.Varjosalo,
A.R.Gehrke,
J.Yan,
S.Talukder,
M.Turunen,
M.Taipale,
H.G.Stunnenberg,
E.Ukkonen,
T.R.Hughes,
M.L.Bulyk,
and
J.Taipale
(2010).
Genome-wide analysis of ETS-family DNA-binding in vitro and in vivo.
|
| |
EMBO J, 29,
2147-2160.
|
|
|
|
|
|
|
K.Kitano,
S.Y.Kim,
and
T.Hakoshima
(2010).
Structural basis for DNA strand separation by the unconventional winged-helix domain of RecQ helicase WRN.
|
| |
Structure, 18,
177-187.
|
|
|
PDB code:
|
|
|
|
|
|
|
|
P.Burda,
P.Laslo,
and
T.Stopka
(2010).
The role of PU.1 and GATA-1 transcription factors during normal and leukemogenic hematopoiesis.
|
| |
Leukemia, 24,
1249-1257.
|
|
|
|
|
|
|
R.Rohs,
X.Jin,
S.M.West,
R.Joshi,
B.Honig,
and
R.S.Mann
(2010).
Origins of specificity in protein-DNA recognition.
|
| |
Annu Rev Biochem, 79,
233-269.
|
|
|
|
|
|
|
T.Shimokawa,
S.Nunomura,
Y.Enomoto,
and
C.Ra
(2010).
Amino acid residues in the beta3 strand and subsequent loop of the conserved ETS domain that mediate basic leucine zipper (bZIP) recruitment and potentially distinguish functional attributes of Ets proteins.
|
| |
Biochem J, 430,
129-139.
|
|
|
|
|
|
|
V.B.Agarkar,
N.D.Babayeva,
P.J.Wilder,
A.Rizzino,
and
T.H.Tahirov
(2010).
Crystal structure of mouse Elf3 C-terminal DNA-binding domain in complex with type II TGF-beta receptor promoter DNA.
|
| |
J Mol Biol, 397,
278-289.
|
|
|
PDB code:
|
|
|
|
|
|
|
|
H.Kamberaj,
and
A.van der Vaart
(2009).
Correlated motions and interactions at the onset of the DNA-induced partial unfolding of Ets-1.
|
| |
Biophys J, 96,
1307-1317.
|
|
|
|
|
|
|
P.Gupta,
G.U.Gurudutta,
D.Saluja,
and
R.P.Tripathi
(2009).
PU.1 and partners: regulation of haematopoietic stem cell fate in normal and malignant haematopoiesis.
|
| |
J Cell Mol Med, 13,
4349-4363.
|
|
|
|
|
|
|
R.Janowski,
S.Panjikar,
A.N.Eddine,
S.H.Kaufmann,
and
M.S.Weiss
(2009).
Structural analysis reveals DNA binding properties of Rv2827c, a hypothetical protein from Mycobacterium tuberculosis.
|
| |
J Struct Funct Genomics, 10,
137-150.
|
|
|
PDB code:
|
|
|
|
|
|
|
|
M.B.Kamath,
I.B.Houston,
A.J.Janovski,
X.Zhu,
S.Gowrisankar,
A.G.Jegga,
and
R.P.DeKoter
(2008).
Dose-dependent repression of T-cell and natural killer cell genes by PU.1 enforces myeloid and B-cell identity.
|
| |
Leukemia, 22,
1214-1225.
|
|
|
|
|
|
|
P.Di Lello,
L.M.Miller Jenkins,
C.Mas,
C.Langlois,
E.Malitskaya,
A.Fradet-Turcotte,
J.Archambault,
P.Legault,
and
J.G.Omichinski
(2008).
p53 and TFIIEalpha share a common binding site on the Tfb1/p62 subunit of TFIIH.
|
| |
Proc Natl Acad Sci U S A, 105,
106-111.
|
|
|
PDB code:
|
|
|
|
|
|
|
|
Y.Suwa,
T.Nakamura,
S.Toma,
S.Ikemizu,
H.Kai,
and
Y.Yamagata
(2008).
Preparation, crystallization and preliminary X-ray diffraction analysis of the DNA-binding domain of the Ets transcription factor in complex with target DNA.
|
| |
Acta Crystallogr Sect F Struct Biol Cryst Commun, 64,
171-174.
|
|
|
|
|
|
|
A.Dakic,
L.Wu,
and
S.L.Nutt
(2007).
Is PU.1 a dosage-sensitive regulator of haemopoietic lineage commitment and leukaemogenesis?
|
| |
Trends Immunol, 28,
108-114.
|
|
|
|
|
|
|
F.Spyrakis,
P.Cozzini,
C.Bertoli,
A.Marabotti,
G.E.Kellogg,
and
A.Mozzarelli
(2007).
Energetics of the protein-DNA-water interaction.
|
| |
BMC Struct Biol, 7,
4.
|
|
|
|
|
|
|
J.C.Kwok,
J.Perdomo,
and
B.H.Chong
(2007).
Identification of a monopartite sequence in PU.1 essential for nuclear import, DNA-binding and transcription of myeloid-specific genes.
|
| |
J Cell Biochem, 101,
1456-1474.
|
|
|
|
|
|
|
T.Kiyota,
A.Kato,
and
Y.Kato
(2007).
Ets-1 regulates radial glia formation during vertebrate embryogenesis.
|
| |
Organogenesis, 3,
93.
|
|
|
|
|
|
|
A.Datta,
U.Sinha-Datta,
N.K.Dhillon,
S.Buch,
and
C.Nicot
(2006).
The HTLV-I p30 interferes with TLR4 signaling and modulates the release of pro- and anti-inflammatory cytokines from human macrophages.
|
| |
J Biol Chem, 281,
23414-23424.
|
|
|
|
|
|
|
C.Guillouf,
I.Gallais,
and
F.Moreau-Gachelin
(2006).
Spi-1/PU.1 oncoprotein affects splicing decisions in a promoter binding-dependent manner.
|
| |
J Biol Chem, 281,
19145-19155.
|
|
|
|
|
|
|
C.W.Liew,
K.D.Rand,
R.J.Simpson,
W.W.Yung,
R.E.Mansfield,
M.Crossley,
M.Proetorius-Ibba,
C.Nerlov,
F.M.Poulsen,
and
J.P.Mackay
(2006).
Molecular analysis of the interaction between the hematopoietic master transcription factors GATA-1 and PU.1.
|
| |
J Biol Chem, 281,
28296-28306.
|
|
|
|
|
|
|
C.Qian,
Q.Zhang,
S.Li,
L.Zeng,
M.J.Walsh,
and
M.M.Zhou
(2005).
Structure and chromosomal DNA binding of the SWIRM domain.
|
| |
Nat Struct Mol Biol, 12,
1078-1085.
|
|
|
PDB codes:
|
|
|
|
|
|
|
|
D.Yaniw,
and
J.Hu
(2005).
Epithelium-specific ets transcription factor 2 upregulates cytokeratin 18 expression in pulmonary epithelial cells through an interaction with cytokeratin 18 intron 1.
|
| |
Cell Res, 15,
423-429.
|
|
|
|
|
|
|
G.M.Lee,
L.W.Donaldson,
M.A.Pufall,
H.S.Kang,
I.Pot,
B.J.Graves,
and
L.P.McIntosh
(2005).
The structural and dynamic basis of Ets-1 DNA binding autoinhibition.
|
| |
J Biol Chem, 280,
7088-7099.
|
|
|
PDB code:
|
|
|
|
|
|
|
|
G.U.Gangenahalli,
P.Gupta,
D.Saluja,
Y.K.Verma,
V.Kishore,
R.Chandra,
R.K.Sharma,
and
T.Ravindranath
(2005).
Stem cell fate specification: role of master regulatory switch transcription factor PU.1 in differential hematopoiesis.
|
| |
Stem Cells Dev, 14,
140-152.
|
|
|
|
|
|
|
J.A.Listman,
N.Wara-aswapati,
J.E.Race,
L.W.Blystone,
N.Walker-Kopp,
Z.Yang,
and
P.E.Auron
(2005).
Conserved ETS domain arginines mediate DNA binding, nuclear localization, and a novel mode of bZIP interaction.
|
| |
J Biol Chem, 280,
41421-41428.
|
|
|
|
|
|
|
L.Aravind,
V.Anantharaman,
S.Balaji,
M.M.Babu,
and
L.M.Iyer
(2005).
The many faces of the helix-turn-helix domain: transcription regulation and beyond.
|
| |
FEMS Microbiol Rev, 29,
231-262.
|
|
|
|
|
|
|
N.Suraweera,
E.Meijne,
J.Moody,
L.G.Carvajal-Carmona,
K.Yoshida,
P.Pollard,
J.Fitzgibbon,
A.Riches,
T.van Laar,
R.Huiskamp,
A.Rowan,
I.P.Tomlinson,
and
A.Silver
(2005).
Mutations of the PU.1 Ets domain are specifically associated with murine radiation-induced, but not human therapy-related, acute myeloid leukaemia.
|
| |
Oncogene, 24,
3678-3683.
|
|
|
|
|
|
|
T.L.Tootle,
and
I.Rebay
(2005).
Post-translational modifications influence transcription factor activity: a view from the ETS superfamily.
|
| |
Bioessays, 27,
285-298.
|
|
|
|
|
|
|
C.Meignin,
B.Dastugue,
and
C.Vaury
(2004).
Intercellular communication between germ line and somatic line is utilized to control the transcription of ZAM, an endogenous retrovirus from Drosophila melanogaster.
|
| |
Nucleic Acids Res, 32,
3799-3806.
|
|
|
|
|
|
|
G.R.Miley,
D.Fantz,
D.Glossip,
X.Lu,
R.M.Saito,
R.E.Palmer,
T.Inoue,
S.Van Den Heuvel,
P.W.Sternberg,
and
K.Kornfeld
(2004).
Identification of residues of the Caenorhabditis elegans LIN-1 ETS domain that are necessary for DNA binding and regulation of vulval cell fates.
|
| |
Genetics, 167,
1697-1709.
|
|
|
|
|
|
|
H.H.Xue,
J.Bollenbacher,
V.Rovella,
R.Tripuraneni,
Y.B.Du,
C.Y.Liu,
A.Williams,
J.P.McCoy,
and
W.J.Leonard
(2004).
GA binding protein regulates interleukin 7 receptor alpha-chain gene expression in T cells.
|
| |
Nat Immunol, 5,
1036-1044.
|
|
|
|
|
|
|
S.Ano,
R.Pereira,
M.Pironin,
I.Lesault,
C.Milley,
I.Lebigot,
C.T.Quang,
and
J.Ghysdael
(2004).
Erythroblast transformation by FLI-1 depends upon its specific DNA binding and transcriptional activation properties.
|
| |
J Biol Chem, 279,
2993-3002.
|
|
|
|
|
|
|
V.A.Roberts,
D.A.Case,
and
V.Tsui
(2004).
Predicting interactions of winged-helix transcription factors with DNA.
|
| |
Proteins, 57,
172-187.
|
|
|
|
|
|
|
A.H.Kwan,
R.Czolij,
J.P.Mackay,
and
M.Crossley
(2003).
Pentaprobe: a comprehensive sequence for the one-step detection of DNA-binding activities.
|
| |
Nucleic Acids Res, 31,
e124.
|
|
|
|
|
|
|
J.Dittmer
(2003).
The biology of the Ets1 proto-oncogene.
|
| |
Mol Cancer, 2,
29.
|
|
|
|
|
|
|
P.E.Shaw,
and
J.Saxton
(2003).
Ternary complex factors: prime nuclear targets for mitogen-activated protein kinases.
|
| |
Int J Biochem Cell Biol, 35,
1210-1226.
|
|
|
|
|
|
|
S.R.McKercher,
C.R.Lombardo,
A.Bobkov,
X.Jia,
and
N.Assa-Munt
(2003).
Identification of a PU.1-IRF4 protein interaction surface predicted by chemical exchange line broadening.
|
| |
Proc Natl Acad Sci U S A, 100,
511-516.
|
|
|
|
|
|
|
A.Verger,
and
M.Duterque-Coquillaud
(2002).
When Ets transcription factors meet their partners.
|
| |
Bioessays, 24,
362-370.
|
|
|
|
|
|
|
C.R.Escalante,
A.L.Brass,
J.M.Pongubala,
E.Shatova,
L.Shen,
H.Singh,
and
A.K.Aggarwal
(2002).
Crystal structure of PU.1/IRF-4/DNA ternary complex.
|
| |
Mol Cell, 10,
1097-1105.
|
|
|
|
|
|
|
G.M.Poon,
P.Gross,
and
R.B.Macgregor
(2002).
The sequence-specific association of the ETS domain of murine PU.1 with DNA exhibits unusual energetics.
|
| |
Biochemistry, 41,
2361-2371.
|
|
|
|
|
|
|
J.Iwahara,
M.Iwahara,
G.W.Daughdrill,
J.Ford,
and
R.T.Clubb
(2002).
The structure of the Dead ringer-DNA complex reveals how AT-rich interaction domains (ARIDs) recognize DNA.
|
| |
EMBO J, 21,
1197-1209.
|
|
|
PDB code:
|
|
|
|
|
|
|
|
M.A.Pufall,
and
B.J.Graves
(2002).
Autoinhibitory domains: modular effectors of cellular regulation.
|
| |
Annu Rev Cell Dev Biol, 18,
421-462.
|
|
|
|
|
|
|
M.K.Anderson,
A.H.Weiss,
G.Hernandez-Hoyos,
C.J.Dionne,
and
E.V.Rothenberg
(2002).
Constitutive expression of PU.1 in fetal hematopoietic progenitors blocks T cell development at the pro-T cell stage.
|
| |
Immunity, 16,
285-296.
|
|
|
|
|
|
|
P.P.Liu,
Y.C.Chen,
C.Li,
Y.H.Hsieh,
S.W.Chen,
S.H.Chen,
W.Y.Jeng,
and
W.J.Chuang
(2002).
Solution structure of the DNA-binding domain of interleukin enhancer binding factor 1 (FOXK1a).
|
| |
Proteins, 49,
543-553.
|
|
|
PDB code:
|
|
|
|
|
|
|
|
S.Marecki,
and
M.J.Fenton
(2002).
The role of IRF-4 in transcriptional regulation.
|
| |
J Interferon Cytokine Res, 22,
121-133.
|
|
|
|
|
|
|
C.J.Hu,
S.Rao,
D.L.Ramirez-Bergeron,
L.A.Garrett-Sinha,
S.Gerondakis,
M.R.Clark,
and
M.C.Simon
(2001).
PU.1/Spi-B regulation of c-rel is essential for mature B cell survival.
|
| |
Immunity, 15,
545-555.
|
|
|
|
|
|
|
C.W.Garvie,
and
C.Wolberger
(2001).
Recognition of specific DNA sequences.
|
| |
Mol Cell, 8,
937-946.
|
|
|
|
|
|
|
A.Buijs,
L.van Rompaey,
A.C.Molijn,
J.N.Davis,
A.C.Vertegaal,
M.D.Potter,
C.Adams,
S.van Baal,
E.C.Zwarthoff,
M.F.Roussel,
and
G.C.Grosveld
(2000).
The MN1-TEL fusion protein, encoded by the translocation (12;22)(p13;q11) in myeloid leukemia, is a transcription factor with transforming activity.
|
| |
Mol Cell Biol, 20,
9281-9293.
|
|
|
|
|
|
|
D.O.Cowley,
and
B.J.Graves
(2000).
Phosphorylation represses Ets-1 DNA binding by reinforcing autoinhibition.
|
| |
Genes Dev, 14,
366-376.
|
|
|
|
|
|
|
J.M.Quinn,
P.Barraco,
M.Eriksson,
and
S.Merchant
(2000).
Coordinate copper- and oxygen-responsive Cyc6 and Cpx1 expression in Chlamydomonas is mediated by the same element.
|
| |
J Biol Chem, 275,
6080-6089.
|
|
|
|
|
|
|
K.Kwon,
and
D.Beckett
(2000).
Function of a conserved sequence motif in biotin holoenzyme synthetases.
|
| |
Protein Sci, 9,
1530-1539.
|
|
|
|
|
|
|
L.D.Williams,
and
L.J.Maher
(2000).
Electrostatic mechanisms of DNA deformation.
|
| |
Annu Rev Biophys Biomol Struct, 29,
497-521.
|
|
|
|
|
|
|
T.L.Goetz,
T.L.Gu,
N.A.Speck,
and
B.J.Graves
(2000).
Auto-inhibition of Ets-1 is counteracted by DNA binding cooperativity with core-binding factor alpha2.
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| |
Mol Cell Biol, 20,
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A.L.Brass,
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Assembly requirements of PU.1-Pip (IRF-4) activator complexes: inhibiting function in vivo using fused dimers.
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EMBO J, 18,
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C.Wolberger
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Multiprotein-DNA complexes in transcriptional regulation.
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F.Karantzoulis-Fegaras,
H.Antoniou,
S.L.Lai,
G.Kulkarni,
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G.K.Wong,
T.L.Miller,
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Characterization of the human endothelial nitric-oxide synthase promoter.
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J Biol Chem, 274,
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F.Pio,
N.Assa-Munt,
J.Yguerabide,
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Mutants of ETS domain PU.1 and GGAA/T recognition: free energies and kinetics.
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| |
Protein Sci, 8,
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G.Jayaraman,
R.Srinivas,
C.Duggan,
E.Ferreira,
S.Swaminathan,
K.Somasundaram,
J.Williams,
C.Hauser,
M.Kurkinen,
R.Dhar,
S.Weitzman,
G.Buttice,
and
B.Thimmapaya
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p300/cAMP-responsive element-binding protein interactions with ets-1 and ets-2 in the transcriptional activation of the human stromelysin promoter.
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J Biol Chem, 274,
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H.E.Xu,
M.A.Rould,
W.Xu,
J.A.Epstein,
R.L.Maas,
and
C.O.Pabo
(1999).
Crystal structure of the human Pax6 paired domain-DNA complex reveals specific roles for the linker region and carboxy-terminal subdomain in DNA binding.
|
| |
Genes Dev, 13,
1263-1275.
|
|
|
PDB code:
|
|
|
|
|
|
|
|
H.Singh,
R.P.DeKoter,
and
J.C.Walsh
(1999).
PU.1, a shared transcriptional regulator of lymphoid and myeloid cell fates.
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| |
Cold Spring Harb Symp Quant Biol, 64,
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K.Nadassy,
S.J.Wodak,
and
J.Janin
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Structural features of protein-nucleic acid recognition sites.
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| |
Biochemistry, 38,
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L.A.Dickinson,
J.W.Trauger,
E.E.Baird,
P.B.Dervan,
B.J.Graves,
and
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Inhibition of Ets-1 DNA binding and ternary complex formation between Ets-1, NF-kappaB, and DNA by a designed DNA-binding ligand.
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J Biol Chem, 274,
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L.Amaravadi,
and
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DNA methylation and chromatin structure regulate PU.1 expression.
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| |
DNA Cell Biol, 18,
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L.Van Rompaey,
W.Dou,
A.Buijs,
and
G.Grosveld
(1999).
Tel, a frequent target of leukemic translocations, induces cellular aggregation and influences expression of extracellular matrix components.
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| |
Neoplasia, 1,
526-536.
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|
|
|
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M.Bemark,
A.Mårtensson,
D.Liberg,
and
T.Leanderson
(1999).
Spi-C, a novel Ets protein that is temporally regulated during B lymphocyte development.
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J Biol Chem, 274,
10259-10267.
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N.Wara-aswapati,
Z.Yang,
W.R.Waterman,
Y.Koyama,
S.Tetradis,
B.K.Choy,
A.C.Webb,
and
P.E.Auron
(1999).
Cytomegalovirus IE2 protein stimulates interleukin 1beta gene transcription via tethering to Spi-1/PU.1.
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| |
Mol Cell Biol, 19,
6803-6814.
|
|
|
|
|
|
|
N.Zheng,
E.Fraenkel,
C.O.Pabo,
and
N.P.Pavletich
(1999).
Structural basis of DNA recognition by the heterodimeric cell cycle transcription factor E2F-DP.
|
| |
Genes Dev, 13,
666-674.
|
|
|
PDB code:
|
|
|
|
|
|
|
|
O.Littlefield,
Y.Korkhin,
and
P.B.Sigler
(1999).
The structural basis for the oriented assembly of a TBP/TFB/promoter complex.
|
| |
Proc Natl Acad Sci U S A, 96,
13668-13673.
|
|
|
PDB code:
|
|
|
|
|
|
|
|
P.Zhang,
G.Behre,
J.Pan,
A.Iwama,
N.Wara-Aswapati,
H.S.Radomska,
P.E.Auron,
D.G.Tenen,
and
Z.Sun
(1999).
Negative cross-talk between hematopoietic regulators: GATA proteins repress PU.1.
|
| |
Proc Natl Acad Sci U S A, 96,
8705-8710.
|
|
|
|
|
|
|
S.Rao,
A.Matsumura,
J.Yoon,
and
M.C.Simon
(1999).
SPI-B activates transcription via a unique proline, serine, and threonine domain and exhibits DNA binding affinity differences from PU.1.
|
| |
J Biol Chem, 274,
11115-11124.
|
|
|
|
|
|
|
S.Rao,
L.A.Garrett-Sinha,
J.Yoon,
and
M.C.Simon
(1999).
The Ets factors PU.1 and Spi-B regulate the transcription in vivo of P2Y10, a lymphoid restricted heptahelical receptor.
|
| |
J Biol Chem, 274,
34245-34252.
|
|
|
|
|
|
|
U.Samanta,
D.Pal,
and
P.Chakrabarti
(1999).
Packing of aromatic rings against tryptophan residues in proteins.
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| |
Acta Crystallogr D Biol Crystallogr, 55,
1421-1427.
|
|
|
|
|
|
|
W.Wheat,
D.Fitzsimmons,
H.Lennox,
S.R.Krautkramer,
L.N.Gentile,
L.P.McIntosh,
and
J.Hagman
(1999).
The highly conserved beta-hairpin of the paired DNA-binding domain is required for assembly of Pax-Ets ternary complexes.
|
| |
Mol Cell Biol, 19,
2231-2241.
|
|
|
|
|
|
|
Y.Fujii,
T.Shimizu,
M.Kusumoto,
Y.Kyogoku,
T.Taniguchi,
and
T.Hakoshima
(1999).
Crystal structure of an IRF-DNA complex reveals novel DNA recognition and cooperative binding to a tandem repeat of core sequences.
|
| |
EMBO J, 18,
5028-5041.
|
|
|
PDB code:
|
|
|
|
|
|
|
|
A.G.Bassuk,
K.P.Barton,
R.T.Anandappa,
M.M.Lu,
and
J.M.Leiden
(1998).
Expression pattern of the Ets-related transcription factor Elf-1.
|
| |
Mol Med, 4,
392-401.
|
|
|
|
|
|
|
B.J.Graves,
D.O.Cowley,
T.L.Goetz,
J.M.Petersen,
M.D.Jonsen,
and
M.E.Gillespie
(1998).
Autoinhibition as a transcriptional regulatory mechanism.
|
| |
Cold Spring Harb Symp Quant Biol, 63,
621-629.
|
|
|
|
|
|
|
C.Davies,
R.B.Gerstner,
D.E.Draper,
V.Ramakrishnan,
and
S.W.White
(1998).
The crystal structure of ribosomal protein S4 reveals a two-domain molecule with an extensive RNA-binding surface: one domain shows structural homology to the ETS DNA-binding motif.
|
| |
EMBO J, 17,
4545-4558.
|
|
|
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C.M.Groft,
S.N.Uljon,
R.Wang,
and
M.H.Werner
(1998).
Structural homology between the Rap30 DNA-binding domain and linker histone H5: implications for preinitiation complex assembly.
|
| |
Proc Natl Acad Sci U S A, 95,
9117-9122.
|
|
|
PDB codes:
|
|
|
|
|
|
|
|
C.Wolberger
(1998).
Combinatorial transcription factors.
|
| |
Curr Opin Genet Dev, 8,
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|
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|
|
|
|
H.Yamamoto,
M.L.Flannery,
S.Kupriyanov,
J.Pearce,
S.R.McKercher,
G.W.Henkel,
R.A.Maki,
Z.Werb,
and
R.G.Oshima
(1998).
Defective trophoblast function in mice with a targeted mutation of Ets2.
|
| |
Genes Dev, 12,
1315-1326.
|
|
|
|
|
|
|
I.L.Ross,
X.Yue,
M.C.Ostrowski,
and
D.A.Hume
(1998).
Interaction between PU.1 and another Ets family transcription factor promotes macrophage-specific Basal transcription initiation.
|
| |
J Biol Chem, 273,
6662-6669.
|
|
|
|
|
|
|
J.P.Schneider,
A.Lombardi,
and
W.F.DeGrado
(1998).
Analysis and design of three-stranded coiled coils and three-helix bundles.
|
| |
Fold Des, 3,
R29-R40.
|
|
|
|
|
|
|
K.R.Ely,
and
R.Kodandapani
(1998).
Ankyrin(g) ETS domains to DNA.
|
| |
Nat Struct Biol, 5,
255-259.
|
|
|
|
|
|
|
L.A.Dickinson,
R.J.Gulizia,
J.W.Trauger,
E.E.Baird,
D.E.Mosier,
J.M.Gottesfeld,
and
P.B.Dervan
(1998).
Inhibition of RNA polymerase II transcription in human cells by synthetic DNA-binding ligands.
|
| |
Proc Natl Acad Sci U S A, 95,
12890-12895.
|
|
|
|
|
|
|
L.A.Tomky,
J.K.Strauss-Soukup,
and
L.J.Maher
(1998).
Effects of phosphate neutralization on the shape of the AP-1 transcription factor binding site in duplex DNA.
|
| |
Nucleic Acids Res, 26,
2298-2305.
|
|
|
|
|
|
|
M.A.Markus,
R.B.Gerstner,
D.E.Draper,
and
D.A.Torchia
(1998).
The solution structure of ribosomal protein S4 delta41 reveals two subdomains and a positively charged surface that may interact with RNA.
|
| |
EMBO J, 17,
4559-4571.
|
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|
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M.F.Smith,
V.S.Carl,
T.Lodie,
and
M.J.Fenton
(1998).
Secretory interleukin-1 receptor antagonist gene expression requires both a PU.1 and a novel composite NF-kappaB/PU.1/ GA-binding protein binding site.
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| |
J Biol Chem, 273,
24272-24279.
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M.Hauses,
R.R.Tönjes,
and
M.Grez
(1998).
The transcription factor Sp1 regulates the myeloid-specific expression of the human hematopoietic cell kinase (HCK) gene through binding to two adjacent GC boxes within the HCK promoter-proximal region.
|
| |
J Biol Chem, 273,
31844-31852.
|
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|
|
N.Potier,
L.J.Donald,
I.Chernushevich,
A.Ayed,
W.Ens,
C.H.Arrowsmith,
K.G.Standing,
and
H.W.Duckworth
(1998).
Study of a noncovalent trp repressor: DNA operator complex by electrospray ionization time-of-flight mass spectrometry.
|
| |
Protein Sci, 7,
1388-1395.
|
|
|
|
|
|
|
P.Gross,
A.A.Yee,
C.H.Arrowsmith,
and
R.B.Macgregor
(1998).
Quantitative hydroxyl radical footprinting reveals cooperative interactions between DNA-binding subdomains of PU.1 and IRF4.
|
| |
Biochemistry, 37,
9802-9811.
|
|
|
|
|
|
|
P.Gross,
C.H.Arrowsmith,
and
R.B.Macgregor
(1998).
Hydroxyl radical footprinting of DNA complexes of the ets domain of PU.1 and its comparison to the crystal structure.
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| |
Biochemistry, 37,
5129-5135.
|
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|
|
|
|
R.C.Fisher,
and
E.W.Scott
(1998).
Role of PU.1 in hematopoiesis.
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| |
Stem Cells, 16,
25-37.
|
|
|
|
|
|
|
R.C.Fisher,
M.C.Olson,
J.M.Pongubala,
J.M.Perkel,
M.L.Atchison,
E.W.Scott,
and
M.C.Simon
(1998).
Normal myeloid development requires both the glutamine-rich transactivation domain and the PEST region of transcription factor PU.1 but not the potent acidic transactivation domain.
|
| |
Mol Cell Biol, 18,
4347-4357.
|
|
|
|
|
|
|
S.A.Woodson,
and
N.B.Leontis
(1998).
Structure and dynamics of ribosomal RNA.
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| |
Curr Opin Struct Biol, 8,
294-300.
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S.Tan,
and
T.J.Richmond
(1998).
Eukaryotic transcription factors.
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| |
Curr Opin Struct Biol, 8,
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Y.Chinenov,
T.Schmidt,
X.Y.Yang,
and
M.E.Martin
(1998).
Identification of redox-sensitive cysteines in GA-binding protein-alpha that regulate DNA binding and heterodimerization.
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| |
J Biol Chem, 273,
6203-6209.
|
|
|
|
|
|
|
Y.Mo,
B.Vaessen,
K.Johnston,
and
R.Marmorstein
(1998).
Structures of SAP-1 bound to DNA targets from the E74 and c-fos promoters: insights into DNA sequence discrimination by Ets proteins.
|
| |
Mol Cell, 2,
201-212.
|
|
|
PDB codes:
|
|
|
|
|
|
|
|
A.D.Sharrocks,
A.L.Brown,
Y.Ling,
and
P.R.Yates
(1997).
The ETS-domain transcription factor family.
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| |
Int J Biochem Cell Biol, 29,
1371-1387.
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A.Herbert,
J.Alfken,
Y.G.Kim,
I.S.Mian,
K.Nishikura,
and
A.Rich
(1997).
A Z-DNA binding domain present in the human editing enzyme, double-stranded RNA adenosine deaminase.
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| |
Proc Natl Acad Sci U S A, 94,
8421-8426.
|
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|
|
|
|
|
C.Jousset,
C.Carron,
A.Boureux,
C.T.Quang,
C.Oury,
I.Dusanter-Fourt,
M.Charon,
J.Levin,
O.Bernard,
and
J.Ghysdael
(1997).
A domain of TEL conserved in a subset of ETS proteins defines a specific oligomerization interface essential to the mitogenic properties of the TEL-PDGFR beta oncoprotein.
|
| |
EMBO J, 16,
69-82.
|
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|
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C.T.Quang,
O.Wessely,
M.Pironin,
H.Beug,
and
J.Ghysdael
(1997).
Cooperation of Spi-1/PU.1 with an activated erythropoietin receptor inhibits apoptosis and Epo-dependent differentiation in primary erythroblasts and induces their Kit ligand-dependent proliferation.
|
| |
EMBO J, 16,
5639-5653.
|
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|
|
|
E.Martínez-Hackert,
and
A.M.Stock
(1997).
The DNA-binding domain of OmpR: crystal structures of a winged helix transcription factor.
|
| |
Structure, 5,
109-124.
|
|
|
PDB code:
|
|
|
|
|
|
|
|
E.Rao,
W.Dang,
G.Tian,
and
R.Sen
(1997).
A three-protein-DNA complex on a B cell-specific domain of the immunoglobulin mu heavy chain gene enhancer.
|
| |
J Biol Chem, 272,
6722-6732.
|
|
|
|
|
|
|
G.Patikoglou,
and
S.K.Burley
(1997).
Eukaryotic transcription factor-DNA complexes.
|
| |
Annu Rev Biophys Biomol Struct, 26,
289-325.
|
|
|
|
|
|
|
J.K.Strauss-Soukup,
and
L.J.Maher
(1997).
Role of asymmetric phosphate neutralization in DNA bending by PU.1.
|
| |
J Biol Chem, 272,
31570-31575.
|
|
|
|
|
|
|
J.K.Strauss-Soukup,
and
L.J.Maher
(1997).
DNA bending by GCN4 mutants bearing cationic residues.
|
| |
Biochemistry, 36,
10026-10032.
|
|
|
|
|
|
|
K.Georgopoulos
(1997).
Transcription factors required for lymphoid lineage commitment.
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| |
Curr Opin Immunol, 9,
222-227.
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|
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M.Sunnerhagen,
M.Nilges,
G.Otting,
and
J.Carey
(1997).
Solution structure of the DNA-binding domain and model for the complex of multifunctional hexameric arginine repressor with DNA.
|
| |
Nat Struct Biol, 4,
819-826.
|
|
|
PDB code:
|
|
|
|
|
|
|
|
P.Oettgen,
R.M.Alani,
M.A.Barcinski,
L.Brown,
Y.Akbarali,
J.Boltax,
C.Kunsch,
K.Munger,
and
T.A.Libermann
(1997).
Isolation and characterization of a novel epithelium-specific transcription factor, ESE-1, a member of the ets family.
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| |
Mol Cell Biol, 17,
4419-4433.
|
|
|
|
|
|
|
R.E.Dickerson,
and
T.K.Chiu
(1997).
Helix bending as a factor in protein/DNA recognition.
|
| |
Biopolymers, 44,
361-403.
|
|
|
|
|
|
|
R.M.Xu,
C.Koch,
Y.Liu,
J.R.Horton,
D.Knapp,
K.Nasmyth,
and
X.Cheng
(1997).
Crystal structure of the DNA-binding domain of Mbp1, a transcription factor important in cell-cycle control of DNA synthesis.
|
| |
Structure, 5,
349-358.
|
|
|
PDB code:
|
|
|
|
|
|
|
|
Y.Xing,
D.Guha Thakurta,
and
D.E.Draper
(1997).
The RNA binding domain of ribosomal protein L11 is structurally similar to homeodomains.
|
| |
Nat Struct Biol, 4,
24-27.
|
|
|
|
|
|
|
F.Pio,
R.Kodandapani,
C.Z.Ni,
W.Shepard,
M.Klemsz,
S.R.McKercher,
R.A.Maki,
and
K.R.Ely
(1996).
New insights on DNA recognition by ets proteins from the crystal structure of the PU.1 ETS domain-DNA complex.
|
| |
J Biol Chem, 271,
23329-23337.
|
|
|
|
|
|
|
P.Shore,
A.J.Whitmarsh,
R.Bhaskaran,
R.J.Davis,
J.P.Waltho,
and
A.D.Sharrocks
(1996).
Determinants of DNA-binding specificity of ETS-domain transcription factors.
|
| |
Mol Cell Biol, 16,
3338-3349.
|
|
|
|
|
|
|
R.G.Oshima,
H.Baribault,
and
C.Caulín
(1996).
Oncogenic regulation and function of keratins 8 and 18.
|
| |
Cancer Metastasis Rev, 15,
445-471.
|
|
|
|
|
|
|
S.R.McKercher,
B.E.Torbett,
K.L.Anderson,
G.W.Henkel,
D.J.Vestal,
H.Baribault,
M.Klemsz,
A.J.Feeney,
G.E.Wu,
C.J.Paige,
and
R.A.Maki
(1996).
Targeted disruption of the PU.1 gene results in multiple hematopoietic abnormalities.
|
| |
EMBO J, 15,
5647-5658.
|
|
|
|
|
|
The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
code is
shown on the right.
|
|
Links
