PDBsum entry: 1pp4

Hydrolase

PDB id: 1pp4

Contents

Protein chains

233 a.a. *

Ligands

NAG ×4

Waters ×59

* Residue conservation analysis

PDB id:

1pp4

Name:

Hydrolase

Title:

The crystal structure of rhamnogalacturonan acetylesterase i group p3121

Structure:

Rhamnogalacturonan acetylesterase. Chain: a, b. Synonym: rgae. Engineered: yes

Source:

Aspergillus aculeatus. Organism_taxid: 5053. Gene: rha1. Expressed in: aspergillus oryzae. Expression_system_taxid: 5062.

Resolution:

2.50Å R-factor: 0.182 R-free: 0.222

Authors:

A.Molgaard,S.Larsen

Key ref:

A.Mølgaard and S.Larsen (2004). Crystal packing in two pH-dependent crystal forms of rhamnogalacturonan acetylesterase. Acta Crystallogr D Biol Crystallogr, 60, 472-478. PubMed id: 14993671 DOI: 10.1107/S0907444903029767

Date:

16-Jun-03 Release date: 02-Mar-04

Protein chains

Q00017  (RHA1_ASPAC) -  Rhamnogalacturonan acetylesterase

Seq: 250 a.a.

Struc: 233 a.a.

Enzyme reactions

• Enzyme class: E.C.3.1.1.86 - Rhamnogalacturonan acetylesterase.

 Gene Ontology (GO) functional annotation 

• Biological process: lipid metabolic process (1 term)
• Biochemical function: hydrolase activity (2 terms)

DOI no: 10.1107/S0907444903029767

Acta Crystallogr D Biol Crystallogr 60:472-478 (2004)

PubMed id: 14993671

Crystal packing in two pH-dependent crystal forms of rhamnogalacturonan acetylesterase.

A.Mølgaard, S.Larsen.

ABSTRACT

The glycoprotein rhamnogalacturonan acetylesterase from Aspergillus aculeatus has been crystallized in two crystal forms, an orthorhombic and a trigonal crystal form. In the orthorhombic crystal form, the covalently bound carbohydrate at one of the two N-glycosylation sites is involved in crystal contacts. The orthorhombic crystal form was obtained at pH 5.0 and the trigonal crystal form at pH 4.5. In one case, the two crystal forms were found in the same drop at pH 4.7. The differences in crystal packing in the two crystal forms can be explained by the pH-dependent variation in the protonation state of the glutamic acid residues on the protein surface.

Selected figure(s)

Figure 3.
Figure 3 An overlay of the orthorhombic (yellow) and the trigonal (purple) crystal form at the trigonal AA and BB crystal contacts. The figure was prepared using the program MOLSCRIPT (Kraulis, 1991[Kraulis, P. (1991). J. Appl. Cryst. 24, 946-950.]).

The above figure is reprinted by permission from the IUCr: Acta Crystallogr D Biol Crystallogr (2004, 60, 472-478) copyright 2004.

Figure was selected by an automated process.