spacer
spacer
Go to PDB code: 
protein Protein-protein interface(s) links
Antifungal protein PDB id
1pcv
Jmol
Contents
Protein chains
205 a.a. *
Waters ×140
* Residue conservation analysis
PDB id:
1pcv
Name: Antifungal protein
Title: Crystal structure of osmotin, a plant antifungal protein
Structure: Osmotin. Chain: a, b. Fragment: residues 1-205
Source: Nicotiana tabacum. Common tobacco. Organism_taxid: 4097. Strain: l. Var. Wisconsin 38. Other_details: salt-adapted cultured cell
Resolution:
2.30Å     R-factor:   0.211     R-free:   0.257
Authors: K.Min,S.C.Ha,D.-J.Yun,R.A.Bressan,K.K.Kim
Key ref:
K.Min et al. (2004). Crystal structure of osmotin, a plant antifungal protein. Proteins, 54, 170-173. PubMed id: 14705035 DOI: 10.1002/prot.10571
Date:
16-May-03     Release date:   17-Feb-04    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
P14170  (OSMO_TOBAC) -  Osmotin
Seq:
Struc: 		246 a.a.
205 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     vacuole   1 term 
  Biological process     response to stress   3 terms 

 

 
DOI no: 10.1002/prot.10571 Proteins 54:170-173 (2004)
PubMed id: 14705035  
 
 
Crystal structure of osmotin, a plant antifungal protein.
K.Min, S.C.Ha, P.M.Hasegawa, R.A.Bressan, D.J.Yun, K.K.Kim.
 
  ABSTRACT  
 

 
  Selected figure(s)  
 
Figure 1.
Figure 1. (A) Ribbon diagram of osmotin viewing along the cleft. The three domains are colored red (domain I), blue (domain II), and yellow (domain III). N- and C-termini are labeled. Residues (Glu84, Asp97, Asp102, and Asp185) around the acidic cleft are drawn in green stick models. The figure was drawn using MOLSCRIPT.[16] (B) Stereo diagram of the superposition of the C traces of osmotin (red), PR-5d (magenta), zeamatin (green), and thaumatin (blue) in the same orientation with (A). An arrow indicates the thaumatin loop in domain I. 		Figure 2.
Figure 2. Surface representation of the clefts of osmotin (A), PR-5d (B), zeamatin (C), and thaumatin (D), depicting the electrostatic potential with a color scale that varies from blue to red, representing positive and negative potential, respectively.
 
  The above figures are reprinted by permission from John Wiley & Sons, Inc.: Proteins (2004, 54, 170-173) copyright 2004.  

Literature references that cite this PDB file's key reference

  PubMed id Reference
21324123 B.Petre, I.Major, N.Rouhier, and S.Duplessis (2011).
Genome-wide analysis of eukaryote thaumatin-like proteins (TLPs) with an emphasis on poplar.
  BMC Plant Biol, 11, 33.  
21311758 M.Miele, S.Costantini, and G.Colonna (2011).
Structural and functional similarities between osmotin from Nicotiana tabacum seeds and human adiponectin.
  PLoS One, 6, e16690.  
20563648 V.L.Soares, S.M.Rodrigues, T.M.de Oliveira, T.O.de Queiroz, L.S.Lima, B.T.Hora-Júnior, K.P.Gramacho, F.Micheli, J.C.Cascardo, W.C.Otoni, A.S.Gesteira, and M.G.Costa (2011).
Unraveling new genes associated with seed development and metabolism in Bixa orellana L. by expressed sequence tag (EST) analysis.
  Mol Biol Rep, 38, 1329-1340.  
20383746 X.Ren, Q.Kong, P.Wang, F.Jiang, H.Wang, T.Yu, and X.Zheng (2011).
Molecular cloning of a PR-5 like protein gene from cherry tomato and analysis of the response of this gene to abiotic stresses.
  Mol Biol Rep, 38, 801-807.  
21046196 Y.M.Tzou, T.S.Huang, K.W.Huggins, B.A.Chin, A.H.Simonne, and N.K.Singh (2011).
Expression of truncated tobacco osmotin in Escherichia coli: purification and antifungal activity.
  Biotechnol Lett, 33, 539-543.  
19997927 J.J.Liu, A.Zamani, and A.K.Ekramoddoullah (2010).
Expression profiling of a complex thaumatin-like protein family in western white pine.
  Planta, 231, 637-651.  
20204373 J.J.Liu, R.Sturrock, and A.K.Ekramoddoullah (2010).
The superfamily of thaumatin-like proteins: its origin, evolution, and expression towards biological function.
  Plant Cell Rep, 29, 419-436.  
20645107 J.P.Zhao, and X.H.Su (2010).
Patterns of molecular evolution and predicted function in thaumatin-like proteins of Populus trichocarpa.
  Planta, 232, 949-962.  
20796310 N.J.Dafoe, B.E.Gowen, and C.P.Constabel (2010).
Thaumatin-like proteins are differentially expressed and localized in phloem tissues of hybrid poplar.
  BMC Plant Biol, 10, 191.  
19066337 N.Y.Villa, B.R.Kupchak, I.Garitaonandia, J.L.Smith, E.Alonso, C.Alford, L.A.Cowart, Y.A.Hannun, and T.J.Lyons (2009).
Sphingolipids function as downstream effectors of a fungal PAQR.
  Mol Pharmacol, 75, 866-875.  
17786545 F.Perri, F.Romitelli, F.Rufini, F.Secundo, E.Di Stasio, B.Giardina, and A.Vitali (2008).
Different structural behaviors evidenced in thaumatin-like proteins: a spectroscopic study.
  Protein J, 27, 13-20.  
18651170 R.Ghosh, and C.Chakrabarti (2008).
Crystal structure analysis of NP24-I: a thaumatin-like protein.
  Planta, 228, 883-890.
PDB code: 2i0w
17086398 S.D'Angeli, and M.M.Altamura (2007).
Osmotin induces cold protection in olive trees by affecting programmed cell death and cytoskeleton organization.
  Planta, 225, 1147-1163.  
16736102 R.G.Shatters, L.M.Boykin, S.L.Lapointe, W.B.Hunter, and A.A.Weathersbee (2006).
Phylogenetic and structural relationships of the PR5 gene family reveal an ancient multigene family conserved in plants and select animal taxa.
  J Mol Evol, 63, 12-29.  
16307370 M.J.Kim, B.K.Ham, H.R.Kim, I.J.Lee, Y.J.Kim, K.H.Ryu, Y.I.Park, and K.H.Paek (2005).
In vitro and in planta interaction evidence between Nicotiana tabacum thaumatin-like protein 1 (TLP1) and cucumber mosaic virus proteins.
  Plant Mol Biol, 59, 981-994.  
15664187 M.L.Narasimhan, M.A.Coca, J.Jin, T.Yamauchi, Y.Ito, T.Kadowaki, K.K.Kim, J.M.Pardo, B.Damsz, P.M.Hasegawa, D.J.Yun, and R.A.Bressan (2005).
Osmotin is a homolog of mammalian adiponectin and controls apoptosis in yeast through a homolog of mammalian adiponectin receptor.
  Mol Cell, 17, 171-180.  
  16510989 Y.Dall'Antonia, T.Pavkov, H.Fuchs, H.Breiteneder, and W.Keller (2005).
Crystallization and preliminary structure determination of the plant food allergen Pru av 2.
  Acta Crystallogr Sect F Struct Biol Cryst Commun, 61, 186-188.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.