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Presynaptic neurotoxin PDB id
1omg
Jmol
Contents
Protein chain
26 a.a.
PDB id:
1omg
Name: Presynaptic neurotoxin
Title: Nmr study of omega-conotoxin mviia
Structure: Omega-conotoxin mviia. Chain: a
Source: Conus magus. Magus cone. Organism_taxid: 6492
NMR struc: 13 models
Authors: T.Kohno,J.-I.Kim,K.Kobayashi,Y.Kodera,T.Maeda,K.Sato
Key ref:
T.Kohno et al. (1995). Three-dimensional structure in solution of the calcium channel blocker omega-conotoxin MVIIA. Biochemistry, 34, 10256-10265. PubMed id: 7640281 DOI: 10.1021/bi00032a020
Date:
26-Apr-95     Release date:   03-Apr-96    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P05484  (CO17A_CONMA) -  Omega-conotoxin MVIIA
Seq:
Struc: 		71 a.a.
26 a.a.*
Key:    PfamA domain  Secondary structure
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     extracellular region   1 term 
  Biological process     pathogenesis   1 term 
  Biochemical function     ion channel inhibitor activity     1 term  

 

 
DOI no: 10.1021/bi00032a020 Biochemistry 34:10256-10265 (1995)
PubMed id: 7640281  
 
 
Three-dimensional structure in solution of the calcium channel blocker omega-conotoxin MVIIA.
T.Kohno, J.I.Kim, K.Kobayashi, Y.Kodera, T.Maeda, K.Sato.
 
  ABSTRACT  
 
The three-dimensional solution structure of omega-conotoxin MVIIA, a 25-mer peptide antagonist of N-type calcium channels, was determined by two-dimensional 1H NMR spectroscopy with simulated annealing calculations. A total of 13 converged structures of omega-conotoxin MVIIA were obtained on the basis of 273 experimental constraints, including 232 distance constraints obtained from nuclear Overhauser effect (NOE) connectivities, 22 torsion angle (phi, chi 1) constraints, and 19 constraints associated with hydrogen bonds and disulfide bonds. The atomic root mean square difference about the averaged coordinate positions is 0.47 +/- 0.08 A for the backbone atoms (N, C alpha, C) and 1.27 +/- 0.14 A for all heavy atoms of the entire peptide. The molecular structure of omega-conotoxin MVIIA is composed of a short triple-stranded antiparallel beta-sheet. The overall beta-sheet topology is +2x, -1, which is the same as that reported for omega-conotoxin GVIA, another N-type calcium channel blocker. The orientation of beta-stranded structure is similar to each other, suggesting that the conserved disulfide bond combination is essential for the molecular folding. We have recently determined by using alanine substitution analyses that Tyr 13 is essential for the activity of both toxins. On the basis of functional and structural analysis, it is shown that both omega-conotoxin MVIIA and GVIA retain a similar conformation to locate Tyr 13 in the appropriate position to allow binding to N-type calcium channels. These results provide a molecular basis for understanding the mechanism of calcium channel modulation through the toxin-channel interaction and insight into the discrimination of different subtypes of calcium channels.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
18435757 H.Kolmar (2008).
Alternative binding proteins: biological activity and therapeutic potential of cystine-knot miniproteins.
  FEBS J, 275, 2684-2690.  
19300539 J.G.McGivern (2007).
Ziconotide: a review of its pharmacology and use in the treatment of pain.
  Neuropsychiatr Dis Treat, 3, 69-85.  
16207099 D.P.Wermeling (2005).
Ziconotide, an intrathecally administered N-type calcium channel antagonist for the treatment of chronic pain.
  Pharmacotherapy, 25, 1084-1094.  
16131759 R.Krätzner, J.E.Debreczeni, T.Pape, T.R.Schneider, A.Wentzel, H.Kolmar, G.M.Sheldrick, and I.Uson (2005).
Structure of Ecballium elaterium trypsin inhibitor II (EETI-II): a rigid molecular scaffold.
  Acta Crystallogr D Biol Crystallogr, 61, 1255-1262.
PDB codes: 1h9h 1h9i 1w7z
14696181 C.Bernard, G.Corzo, S.Adachi-Akahane, G.Foures, K.Kanemaru, Y.Furukawa, T.Nakajima, and H.Darbon (2004).
Solution structure of ADO1, a toxin extracted from the saliva of the assassin bug, Agriosphodrus dohrni.
  Proteins, 54, 195-205.
PDB code: 1lmr
15308644 H.W.Tedford, N.Gilles, A.Ménez, C.J.Doering, G.W.Zamponi, and G.F.King (2004).
Scanning mutagenesis of omega-atracotoxin-Hv1a reveals a spatially restricted epitope that confers selective activity against insect calcium channels.
  J Biol Chem, 279, 44133-44140.  
14976206 L.Volpon, H.Lamthanh, J.Barbier, N.Gilles, J.Molgó, A.Ménez, and J.M.Lancelin (2004).
NMR solution structures of delta-conotoxin EVIA from Conus ermineus that selectively acts on vertebrate neuronal Na+ channels.
  J Biol Chem, 279, 21356-21366.
PDB codes: 1g1p 1g1z
12654924 Z.P.Feng, C.J.Doering, R.J.Winkfein, A.M.Beedle, J.D.Spafford, and G.W.Zamponi (2003).
Determinants of inhibition of transiently expressed voltage-gated calcium channels by omega-conotoxins GVIA and MVIIA.
  J Biol Chem, 278, 20171-20178.  
11344322 D.P.Goldenberg, R.E.Koehn, D.E.Gilbert, and G.Wagner (2001).
Solution structure and backbone dynamics of an omega-conotoxin precursor.
  Protein Sci, 10, 538-550.
PDB code: 1feo
11478874 S.Ludvigsen, L.Thim, A.M.Blom, and B.S.Wulff (2001).
Solution structure of the satiety factor, CART, reveals new functionality of a well-known fold.
  Biochemistry, 40, 9082-9088.
PDB code: 1hy9
10822250 K.J.Nielsen, T.Schroeder, and R.Lewis (2000).
Structure-activity relationships of omega-conotoxins at N-type voltage-sensitive calcium channels.
  J Mol Recognit, 13, 55-70.  
11101291 K.Kobayashi, T.Sasaki, K.Sato, and T.Kohno (2000).
Three-dimensional solution structure of omega-conotoxin TxVII, an L-type calcium channel blocker.
  Biochemistry, 39, 14761-14767.
PDB code: 1f3k
10747778 R.A.Atkinson, B.Kieffer, A.Dejaegere, F.Sirockin, and J.F.Lefèvre (2000).
Structural and dynamic characterization of omega-conotoxin MVIIA: the binding loop exhibits slow conformational exchange.
  Biochemistry, 39, 3908-3919.
PDB codes: 1dw4 1dw5
10714496 S.Menzler, J.A.Bikker, N.Suman-Chauhan, and D.C.Horwell (2000).
Design and biological evaluation of non-peptide analogues of omega-conotoxin MVIIA.
  Bioorg Med Chem Lett, 10, 345-347.  
10409654 A.Wentzel, A.Christmann, R.Krätzner, and H.Kolmar (1999).
Sequence requirements of the GPNG beta-turn of the Ecballium elaterium trypsin inhibitor II explored by combinatorial library screening.
  J Biol Chem, 274, 21037-21043.  
10081964 B.Gilquin, A.Lecoq, F.Desné, M.Guenneugues, S.Zinn-Justin, and A.Ménez (1999).
Conformational and functional variability supported by the BPTI fold: solution structure of the Ca2+ channel blocker calcicludine.
  Proteins, 34, 520-532.
PDB code: 1bf0
10346894 K.J.Nielsen, D.A.Adams, P.F.Alewood, R.J.Lewis, L.Thomas, T.Schroeder, and D.J.Craik (1999).
Effects of chirality at Tyr13 on the structure-activity relationships of omega-conotoxins from Conus magus.
  Biochemistry, 38, 6741-6751.  
9657699 M.Price-Carter, M.S.Hull, and D.P.Goldenberg (1998).
Roles of individual disulfide bonds in the stability and folding of an omega-conotoxin.
  Biochemistry, 37, 9851-9861.  
9548922 P.Savarin, M.Guenneugues, B.Gilquin, H.Lamthanh, S.Gasparini, S.Zinn-Justin, and A.Ménez (1998).
Three-dimensional structure of kappa-conotoxin PVIIA, a novel potassium channel-blocking toxin from cone snails.
  Biochemistry, 37, 5407-5416.
PDB code: 1kcp
9188685 A.C.Rigby, J.D.Baleja, B.C.Furie, and B.Furie (1997).
Three-dimensional structure of a gamma-carboxyglutamic acid-containing conotoxin, conantokin G, from the marine snail Conus geographus: the metal-free conformer.
  Biochemistry, 36, 6906-6914.
PDB code: 1ad7
9228949 J.I.Fletcher, R.Smith, S.I.O'Donoghue, M.Nilges, M.Connor, M.E.Howden, M.J.Christie, and G.F.King (1997).
The structure of a novel insecticidal neurotoxin, omega-atracotoxin-HV1, from the venom of an Australian funnel web spider.
  Nat Struct Biol, 4, 559-566.
PDB code: 1axh
9048550 K.H.Han, K.J.Hwang, S.M.Kim, S.K.Kim, W.R.Gray, B.M.Olivera, J.Rivier, and K.J.Shon (1997).
NMR structure determination of a novel conotoxin, [Pro 7,13] alpha A-conotoxin PIVA.
  Biochemistry, 36, 1669-1677.
PDB code: 1p1p
8952508 M.Price-Carter, W.R.Gray, and D.P.Goldenberg (1996).
Folding of omega-conotoxins. 1. Efficient disulfide-coupled folding of mature sequences in vitro.
  Biochemistry, 35, 15537-15546.  
8952509 M.Price-Carter, W.R.Gray, and D.P.Goldenberg (1996).
Folding of omega-conotoxins. 2. Influence of precursor sequences and protein disulfide isomerase.
  Biochemistry, 35, 15547-15557.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.