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Contents |
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127 a.a.
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127 a.a.
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127 a.a.
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128 a.a.
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* Residue conservation analysis
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PDB id:
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Ion channel/receptor
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Title:
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Structure of acetylcholine receptor pore from electron images
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Structure:
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Acetylcholine receptor protein, alpha chain. Chain: a, d. Fragment: membrane-spanning domain, residues 235-461. Synonym: nicotinic acetylcholine receptor. Acetylcholine receptor protein, beta chain. Chain: b. Fragment: membrane-spanning domain, residues 241-490. Synonym: nicotinic acetylcholine receptor. Acetylcholine receptor protein, delta chain.
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Source:
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Torpedo marmorata. Marbled electric ray. Organism_taxid: 7788. Organ: electric organ. Tissue: derived from muscle. Tissue: derived from muscle
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Biol. unit:
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Pentamer (from
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Authors:
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A.Miyazawa,Y.Fujiyoshi,N.Unwin
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Key ref:
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A.Miyazawa
et al.
(2003).
Structure and gating mechanism of the acetylcholine receptor pore.
Nature,
423,
949-955.
PubMed id:
DOI:
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Date:
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24-Mar-03
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Release date:
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26-Jun-03
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PROCHECK
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Headers
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References
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P02711
(ACHA_TORMA) -
Acetylcholine receptor subunit alpha
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Seq:
Struc:
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461 a.a.
127 a.a.*
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P02712
(ACHB_TORCA) -
Acetylcholine receptor subunit beta
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Seq:
Struc:
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493 a.a.
127 a.a.*
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Gene Ontology (GO) functional annotation
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Cellular component
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membrane
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2 terms
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Biological process
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ion transport
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1 term
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DOI no:
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Nature
423:949-955
(2003)
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PubMed id:
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Structure and gating mechanism of the acetylcholine receptor pore.
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A.Miyazawa,
Y.Fujiyoshi,
N.Unwin.
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ABSTRACT
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The nicotinic acetylcholine receptor controls electrical signalling between
nerve and muscle cells by opening and closing a gated, membrane-spanning pore.
Here we present an atomic model of the closed pore, obtained by electron
microscopy of crystalline postsynaptic membranes. The pore is shaped by an inner
ring of 5 alpha-helices, which curve radially to create a tapering path for the
ions, and an outer ring of 15 alpha-helices, which coil around each other and
shield the inner ring from the lipids. The gate is a constricting hydrophobic
girdle at the middle of the lipid bilayer, formed by weak interactions between
neighbouring inner helices. When acetylcholine enters the ligand-binding domain,
it triggers rotations of the protein chains on opposite sides of the entrance to
the pore. These rotations are communicated through the inner helices, and open
the pore by breaking the girdle apart.
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Selected figure(s)
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Figure 1.
Figure 1: Cross-section of a tubular crystal, at low resolution.
The receptor protein projects from either side of the
membrane, visible as two concentric rings of density, 30 Å
apart. A single receptor, cut centrally, is shown at the top.
The membrane-spanning pore and the N-terminal ligand-binding
domain, shaping a large central vestibule, are outlined by red
and green rectangles, respectively. The surfaces encompassing
the hydrophobic core of the membrane are assumed to lie along
the centres of the rings of density48.
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Figure 6.
Figure 6: Proposed model for the gating mechanism. The
ACh-induced rotations in the  -subunits8
are transmitted to the gate--a hydrophobic barrier to ion
permeation--through the M2 helices. The rotations destabilize
the gate, causing the helices to adopt an alternative
configuration which is permeable to the ions. The helices move
freely during gating because they are mainly separated from the
outer protein wall and connected to it by flexible loops,
containing glycine residues (G). S-S is the disulphide-bridge
pivot in the ligand-binding domain, which is anchored to the
fixed outer shell of the pore. The relevant moving parts are
shaded.
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The above figures are
reprinted
by permission from Macmillan Publishers Ltd:
Nature
(2003,
423,
949-955)
copyright 2003.
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Figures were
selected
by the author.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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B.D.Drever,
G.Riedel,
and
B.Platt
(2011).
The cholinergic system and hippocampal plasticity.
|
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Behav Brain Res, 221,
505-514.
|
|
|
|
|
|
|
H.Nury,
C.Van Renterghem,
Y.Weng,
A.Tran,
M.Baaden,
V.Dufresne,
J.P.Changeux,
J.M.Sonner,
M.Delarue,
and
P.J.Corringer
(2011).
X-ray structures of general anaesthetics bound to a pentameric ligand-gated ion channel.
|
| |
Nature, 469,
428-431.
|
|
|
|
|
|
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J.E.Baenziger,
and
P.J.Corringer
(2011).
3D structure and allosteric modulation of the transmembrane domain of pentameric ligand-gated ion channels.
|
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Neuropharmacology, 60,
116-125.
|
|
|
|
|
|
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S.M.Hanson,
and
C.Czajkowski
(2011).
Disulphide trapping of the GABA(A) receptor reveals the importance of the coupling interface in the action of benzodiazepines.
|
| |
Br J Pharmacol, 162,
673-687.
|
|
|
|
|
|
|
T.K.Machu
(2011).
Therapeutics of 5-HT3 receptor antagonists: current uses and future directions.
|
| |
Pharmacol Ther, 130,
338-347.
|
|
|
|
|
|
|
W.Zheng,
and
A.Auerbach
(2011).
Decrypting the sequence of structural events during the gating transition of pentameric ligand-gated ion channels based on an interpolated elastic network model.
|
| |
PLoS Comput Biol, 7,
e1001046.
|
|
|
|
|
|
|
A.B.Waight,
J.Love,
and
D.N.Wang
(2010).
Structure and mechanism of a pentameric formate channel.
|
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Nat Struct Mol Biol, 17,
31-37.
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PDB codes:
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A.J.Thompson,
H.A.Lester,
and
S.C.Lummis
(2010).
The structural basis of function in Cys-loop receptors.
|
| |
Q Rev Biophys, 43,
449-499.
|
|
|
|
|
|
|
A.Lampert,
A.O.O'Reilly,
P.Reeh,
and
A.Leffler
(2010).
Sodium channelopathies and pain.
|
| |
Pflugers Arch, 460,
249-263.
|
|
|
|
|
|
|
G.Brannigan,
D.N.LeBard,
J.Hénin,
R.G.Eckenhoff,
and
M.L.Klein
(2010).
Multiple binding sites for the general anesthetic isoflurane identified in the nicotinic acetylcholine receptor transmembrane domain.
|
| |
Proc Natl Acad Sci U S A, 107,
14122-14127.
|
|
|
|
|
|
|
G.Y.Liu,
X.L.Ju,
and
J.Cheng
(2010).
Selectivity of Imidacloprid for fruit fly versus rat nicotinic acetylcholine receptors by molecular modeling.
|
| |
J Mol Model, 16,
993.
|
|
|
|
|
|
|
H.Leonov,
and
I.T.Arkin
(2010).
pH-driven helix rotations in the influenza M2 H+ channel: a potential gating mechanism.
|
| |
Eur Biophys J, 39,
1043-1049.
|
|
|
|
|
|
|
H.X.Zhou,
and
J.A.McCammon
(2010).
The gates of ion channels and enzymes.
|
| |
Trends Biochem Sci, 35,
179-185.
|
|
|
|
|
|
|
H.Y.Liao,
and
J.Frank
(2010).
Definition and estimation of resolution in single-particle reconstructions.
|
| |
Structure, 18,
768-775.
|
|
|
|
|
|
|
I.Bahar,
T.R.Lezon,
A.Bakan,
and
I.H.Shrivastava
(2010).
Normal mode analysis of biomolecular structures: functional mechanisms of membrane proteins.
|
| |
Chem Rev, 110,
1463-1497.
|
|
|
|
|
|
|
I.McGonigle,
and
S.C.Lummis
(2010).
Molecular characterization of agonists that bind to an insect GABA receptor.
|
| |
Biochemistry, 49,
2897-2902.
|
|
|
|
|
|
|
J.D.Otero-Cruz,
C.A.Báez-Pagán,
L.Dorna-Pérez,
G.E.Grajales-Reyes,
R.T.Ramírez-Ordoñez,
C.A.Luciano,
C.M.Gómez,
and
J.A.Lasalde-Dominicci
(2010).
Decoding pathogenesis of slow-channel congenital myasthenic syndromes using recombinant expression and mice models.
|
| |
P R Health Sci J, 29,
4.
|
|
|
|
|
|
|
J.M.Cederholm,
N.L.Absalom,
S.Sugiharto,
R.Griffith,
P.R.Schofield,
and
T.M.Lewis
(2010).
Conformational changes in extracellular loop 2 associated with signal transduction in the glycine receptor.
|
| |
J Neurochem, 115,
1245-1255.
|
|
|
|
|
|
|
J.P.Changeux
(2010).
Allosteric receptors: from electric organ to cognition.
|
| |
Annu Rev Pharmacol Toxicol, 50,
1.
|
|
|
|
|
|
|
J.Walstab,
G.Rappold,
and
B.Niesler
(2010).
5-HT(3) receptors: role in disease and target of drugs.
|
| |
Pharmacol Ther, 128,
146-169.
|
|
|
|
|
|
|
K.R.Vinothkumar,
and
R.Henderson
(2010).
Structures of membrane proteins.
|
| |
Q Rev Biophys, 43,
65.
|
|
|
|
|
|
|
K.Yoshimura,
and
M.Sokabe
(2010).
Mechanosensitivity of ion channels based on protein-lipid interactions.
|
| |
J R Soc Interface, 7,
S307-S320.
|
|
|
|
|
|
|
M.Aldea,
M.Castillo,
J.Mulet,
S.Sala,
M.Criado,
and
F.Sala
(2010).
Role of the extracellular transmembrane domain interface in gating and pharmacology of a heteromeric neuronal nicotinic receptor.
|
| |
J Neurochem, 113,
1036-1045.
|
|
|
|
|
|
|
M.L.Baker,
J.Zhang,
S.J.Ludtke,
and
W.Chiu
(2010).
Cryo-EM of macromolecular assemblies at near-atomic resolution.
|
| |
Nat Protoc, 5,
1697-1708.
|
|
|
|
|
|
|
N.Ishii
(2010).
Investigation on stability of transporter protein, glucuronide transporter from Escherichia coli.
|
| |
J Membr Biol, 235,
63-72.
|
|
|
|
|
|
|
P.S.Miller,
and
T.G.Smart
(2010).
Binding, activation and modulation of Cys-loop receptors.
|
| |
Trends Pharmacol Sci, 31,
161-174.
|
|
|
|
|
|
|
S.M.Sine,
H.L.Wang,
S.Hansen,
and
P.Taylor
(2010).
On the origin of ion selectivity in the Cys-loop receptor family.
|
| |
J Mol Neurosci, 40,
70-76.
|
|
|
|
|
|
|
S.Majd,
E.C.Yusko,
Y.N.Billeh,
M.X.Macrae,
J.Yang,
and
M.Mayer
(2010).
Applications of biological pores in nanomedicine, sensing, and nanoelectronics.
|
| |
Curr Opin Biotechnol, 21,
439-476.
|
|
|
|
|
|
|
T.Collins,
and
N.S.Millar
(2010).
Nicotinic acetylcholine receptor transmembrane mutations convert ivermectin from a positive to a negative allosteric modulator.
|
| |
Mol Pharmacol, 78,
198-204.
|
|
|
|
|
|
|
T.Fujii,
A.H.Iwane,
T.Yanagida,
and
K.Namba
(2010).
Direct visualization of secondary structures of F-actin by electron cryomicroscopy.
|
| |
Nature, 467,
724-728.
|
|
|
PDB code:
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|
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|
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|
T.Nakagawa
(2010).
The biochemistry, ultrastructure, and subunit assembly mechanism of AMPA receptors.
|
| |
Mol Neurobiol, 42,
161-184.
|
|
|
|
|
|
|
X.L.Ju,
S.Fusazaki,
H.Hishinuma,
X.Qiao,
I.Ikeda,
and
Y.Ozoe
(2010).
Synthesis and structure-activity relationship analysis of bicyclophosphorothionate blockers with selectivity for housefly gamma-aminobutyric acid receptor channels.
|
| |
Pest Manag Sci, 66,
1002-1010.
|
|
|
|
|
|
|
A.Taly,
P.J.Corringer,
D.Guedin,
P.Lestage,
and
J.P.Changeux
(2009).
Nicotinic receptors: allosteric transitions and therapeutic targets in the nervous system.
|
| |
Nat Rev Drug Discov, 8,
733-750.
|
|
|
|
|
|
|
A.V.Pischalnikova,
and
O.S.Sokolova
(2009).
The domain and conformational organization in potassium voltage-gated ion channels.
|
| |
J Neuroimmune Pharmacol, 4,
71-82.
|
|
|
|
|
|
|
B.H.Lee,
S.H.Choi,
M.K.Pyo,
T.J.Shin,
S.H.Hwang,
B.R.Kim,
S.M.Lee,
J.H.Lee,
J.H.Lee,
H.S.Lee,
H.Choe,
K.H.Han,
H.C.Kim,
H.Rhim,
J.H.Yong,
and
S.Y.Nah
(2009).
A role for Leu247 residue within transmembrane domain 2 in ginsenoside-mediated alpha7 nicotinic acetylcholine receptor regulation.
|
| |
Mol Cells, 27,
591-599.
|
|
|
|
|
|
|
C.J.daCosta,
and
J.E.Baenziger
(2009).
A lipid-dependent uncoupled conformation of the acetylcholine receptor.
|
| |
J Biol Chem, 284,
17819-17825.
|
|
|
|
|
|
|
C.Melis,
G.Bussi,
S.C.Lummis,
and
C.Molteni
(2009).
Trans-cis Switching Mechanisms in Proline Analogues and Their Relevance for the Gating of the 5-HT(3) Receptor.
|
| |
J Phys Chem B, 113,
12148-12153.
|
|
|
|
|
|
|
C.Song,
and
B.Corry
(2009).
Computational study of the transmembrane domain of the acetylcholine receptor.
|
| |
Eur Biophys J, 38,
961-970.
|
|
|
|
|
|
|
D.A.Decker,
and
J.J.Galligan
(2009).
Cross-inhibition between nicotinic acetylcholine receptors and P2X receptors in myenteric neurons and HEK-293 cells.
|
| |
Am J Physiol Gastrointest Liver Physiol, 296,
G1267-G1276.
|
|
|
|
|
|
|
D.C.Chiara,
A.K.Hamouda,
M.R.Ziebell,
L.A.Mejia,
G.Garcia,
and
J.B.Cohen
(2009).
[(3)H]chlorpromazine photolabeling of the torpedo nicotinic acetylcholine receptor identifies two state-dependent binding sites in the ion channel.
|
| |
Biochemistry, 48,
10066-10077.
|
|
|
|
|
|
|
E.K.Millers,
M.Trabi,
P.P.Masci,
M.F.Lavin,
J.de Jersey,
and
L.W.Guddat
(2009).
Crystal structure of textilinin-1, a Kunitz-type serine protease inhibitor from the venom of the Australian common brown snake (Pseudonaja textilis).
|
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FEBS J, 276,
3163-3175.
|
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PDB code:
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E.Luttmann,
J.Ludwig,
A.Höffle-Maas,
M.Samochocki,
A.Maelicke,
and
G.Fels
(2009).
Structural model for the binding sites of allosterically potentiating ligands on nicotinic acetylcholine receptors.
|
| |
ChemMedChem, 4,
1874-1882.
|
|
|
|
|
|
|
F.Orsini,
M.Santacroce,
P.Arosio,
M.Castagna,
C.Lenardi,
G.Poletti,
and
F.V.Sacchi
(2009).
Intermittent contact mode AFM investigation of native plasma membrane of Xenopus laevis oocyte.
|
| |
Eur Biophys J, 38,
903-910.
|
|
|
|
|
|
|
F.Zheng,
L.P.Dwoskin,
P.A.Crooks,
and
C.G.Zhan
(2009).
First-Principles Determination of Molecular Conformations of Indolizidine (-)-235B' in Solution.
|
| |
Theor Chem Acc, 124,
269-278.
|
|
|
|
|
|
|
F.Zhu,
and
G.Hummer
(2009).
Gating transition of pentameric ligand-gated ion channels.
|
| |
Biophys J, 97,
2456-2463.
|
|
|
|
|
|
|
G.Spitzmaul,
F.Gumilar,
J.P.Dilger,
and
C.Bouzat
(2009).
The local anaesthetics proadifen and adiphenine inhibit nicotinic receptors by different molecular mechanisms.
|
| |
Br J Pharmacol, 157,
804-817.
|
|
|
|
|
|
|
H.R.Arias,
F.Gumilar,
A.Rosenberg,
K.M.Targowska-Duda,
D.Feuerbach,
K.Jozwiak,
R.Moaddel,
I.W.Wainer,
and
C.Bouzat
(2009).
Interaction of bupropion with muscle-type nicotinic acetylcholine receptors in different conformational states.
|
| |
Biochemistry, 48,
4506-4518.
|
|
|
|
|
|
|
H.Wang,
Y.Sugiyama,
T.Hikima,
E.Sugano,
H.Tomita,
T.Takahashi,
T.Ishizuka,
and
H.Yawo
(2009).
Molecular determinants differentiating photocurrent properties of two channelrhodopsins from chlamydomonas.
|
| |
J Biol Chem, 284,
5685-5696.
|
|
|
|
|
|
|
I.Derler,
M.Fahrner,
O.Carugo,
M.Muik,
J.Bergsmann,
R.Schindl,
I.Frischauf,
S.Eshaghi,
and
C.Romanin
(2009).
Increased hydrophobicity at the N terminus/membrane interface impairs gating of the severe combined immunodeficiency-related ORAI1 mutant.
|
| |
J Biol Chem, 284,
15903-15915.
|
|
|
|
|
|
|
J.A.Paulo,
and
E.Hawrot
(2009).
Effect of homologous serotonin receptor loop substitutions on the heterologous expression in Pichia of a chimeric acetylcholine-binding protein with alpha-bungarotoxin-binding activity.
|
| |
Protein Expr Purif, 67,
76-81.
|
|
|
|
|
|
|
J.Cheng,
X.L.Ju,
X.Y.Chen,
and
G.Y.Liu
(2009).
Homology modeling of human alpha 1 beta 2 gamma 2 and house fly beta 3 GABA receptor channels and Surflex-docking of fipronil.
|
| |
J Mol Model, 15,
1145-1153.
|
|
|
|
|
|
|
J.Manor,
P.Mukherjee,
Y.S.Lin,
H.Leonov,
J.L.Skinner,
M.T.Zanni,
and
I.T.Arkin
(2009).
Gating mechanism of the influenza A M2 channel revealed by 1D and 2D IR spectroscopies.
|
| |
Structure, 17,
247-254.
|
|
|
|
|
|
|
J.Pierson,
M.Sani,
C.Tomova,
S.Godsave,
and
P.J.Peters
(2009).
Toward visualization of nanomachines in their native cellular environment.
|
| |
Histochem Cell Biol, 132,
253-262.
|
|
|
|
|
|
|
J.Taranda,
S.F.Maison,
J.A.Ballestero,
E.Katz,
J.Savino,
D.E.Vetter,
J.Boulter,
M.C.Liberman,
P.A.Fuchs,
and
A.B.Elgoyhen
(2009).
A point mutation in the hair cell nicotinic cholinergic receptor prolongs cochlear inhibition and enhances noise protection.
|
| |
PLoS Biol, 7,
e18.
|
|
|
|
|
|
|
K.Abe,
K.Tani,
T.Nishizawa,
and
Y.Fujiyoshi
(2009).
Inter-subunit interaction of gastric H+,K+-ATPase prevents reverse reaction of the transport cycle.
|
| |
EMBO J, 28,
1637-1643.
|
|
|
PDB code:
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|
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|
|
|
K.Matsuda,
S.Kanaoka,
M.Akamatsu,
and
D.B.Sattelle
(2009).
Diverse actions and target-site selectivity of neonicotinoids: structural insights.
|
| |
Mol Pharmacol, 76,
1.
|
|
|
|
|
|
|
K.Mio,
T.Ogura,
T.Yamamoto,
Y.Hiroaki,
Y.Fujiyoshi,
Y.Kubo,
and
C.Sato
(2009).
Reconstruction of the P2X(2) receptor reveals a vase-shaped structure with lateral tunnels above the membrane.
|
| |
Structure, 17,
266-275.
|
|
|
|
|
|
|
K.R.Gleitsman,
H.A.Lester,
and
D.A.Dougherty
(2009).
Probing the role of backbone hydrogen bonding in a critical beta sheet of the extracellular domain of a cys-loop receptor.
|
| |
Chembiochem, 10,
1385-1391.
|
|
|
|
|
|
|
K.R.Gleitsman,
J.A.Shanata,
S.J.Frazier,
H.A.Lester,
and
D.A.Dougherty
(2009).
Long-range coupling in an allosteric receptor revealed by mutant cycle analysis.
|
| |
Biophys J, 96,
3168-3178.
|
|
|
|
|
|
|
L.S.Vedula,
G.Brannigan,
N.J.Economou,
J.Xi,
M.A.Hall,
R.Liu,
M.J.Rossi,
W.P.Dailey,
K.C.Grasty,
M.L.Klein,
R.G.Eckenhoff,
and
P.J.Loll
(2009).
A unitary anesthetic binding site at high resolution.
|
| |
J Biol Chem, 284,
24176-24184.
|
|
|
PDB codes:
|
|
|
|
|
|
|
|
M.Hattori,
N.Iwase,
N.Furuya,
Y.Tanaka,
T.Tsukazaki,
R.Ishitani,
M.E.Maguire,
K.Ito,
A.Maturana,
and
O.Nureki
(2009).
Mg(2+)-dependent gating of bacterial MgtE channel underlies Mg(2+) homeostasis.
|
| |
EMBO J, 28,
3602-3612.
|
|
|
PDB code:
|
|
|
|
|
|
|
|
M.Samsó,
W.Feng,
I.N.Pessah,
and
P.D.Allen
(2009).
Coordinated movement of cytoplasmic and transmembrane domains of RyR1 upon gating.
|
| |
PLoS Biol, 7,
e85.
|
|
|
|
|
|
|
M.Zouridakis,
P.Zisimopoulou,
K.Poulas,
and
S.J.Tzartos
(2009).
Recent advances in understanding the structure of nicotinic acetylcholine receptors.
|
| |
IUBMB Life, 61,
407-423.
|
|
|
|
|
|
|
M.Zwolak,
J.Lagerqvist,
and
M.Di Ventra
(2009).
Quantized ionic conductance in nanopores.
|
| |
Phys Rev Lett, 103,
128102.
|
|
|
|
|
|
|
N.Bocquet,
H.Nury,
M.Baaden,
C.Le Poupon,
J.P.Changeux,
M.Delarue,
and
P.J.Corringer
(2009).
X-ray structure of a pentameric ligand-gated ion channel in an apparently open conformation.
|
| |
Nature, 457,
111-114.
|
|
|
PDB code:
|
|
|
|
|
|
|
|
N.L.Absalom,
P.R.Schofield,
and
T.M.Lewis
(2009).
Pore structure of the Cys-loop ligand-gated ion channels.
|
| |
Neurochem Res, 34,
1805-1815.
|
|
|
|
|
|
|
R.J.Hilf,
and
R.Dutzler
(2009).
Structure of a potentially open state of a proton-activated pentameric ligand-gated ion channel.
|
| |
Nature, 457,
115-118.
|
|
|
PDB codes:
|
|
|
|
|
|
|
|
R.J.Law,
and
F.C.Lightstone
(2009).
Modeling neuronal nicotinic and GABA receptors: important interface salt-links and protein dynamics.
|
| |
Biophys J, 97,
1586-1594.
|
|
|
|
|
|
|
R.Pantoja,
E.A.Rodriguez,
M.I.Dibas,
D.A.Dougherty,
and
H.A.Lester
(2009).
Single-molecule imaging of a fluorescent unnatural amino Acid incorporated into nicotinic receptors.
|
| |
Biophys J, 96,
226-237.
|
|
|
|
|
|
|
S.A.Pless,
and
J.W.Lynch
(2009).
Distinct conformational changes in activated agonist-bound and agonist-free glycine receptor subunits.
|
| |
J Neurochem, 108,
1585-1594.
|
|
|
|
|
|
|
S.A.Pless,
and
J.W.Lynch
(2009).
Magnitude of a conformational change in the glycine receptor beta1-beta2 loop is correlated with agonist efficacy.
|
| |
J Biol Chem, 284,
27370-27376.
|
|
|
|
|
|
|
S.Maeda,
S.Nakagawa,
M.Suga,
E.Yamashita,
A.Oshima,
Y.Fujiyoshi,
and
T.Tsukihara
(2009).
Structure of the connexin 26 gap junction channel at 3.5 A resolution.
|
| |
Nature, 458,
597-602.
|
|
|
PDB code:
|
|
|
|
|
|
|
|
S.Raunser,
and
T.Walz
(2009).
Electron crystallography as a technique to study the structure on membrane proteins in a lipidic environment.
|
| |
Annu Rev Biophys, 38,
89.
|
|
|
|
|
|
|
S.S.Smith,
C.Aoki,
and
H.Shen
(2009).
Puberty, steroids and GABA(A) receptor plasticity.
|
| |
Psychoneuroendocrinology, 34,
S91.
|
|
|
|
|
|
|
V.H.Ramey,
H.W.Wang,
and
E.Nogales
(2009).
Ab initio reconstruction of helical samples with heterogeneity, disorder and coexisting symmetries.
|
| |
J Struct Biol, 167,
97.
|
|
|
|
|
|
|
V.Y.Moiseenkova-Bell,
and
T.G.Wensel
(2009).
Hot on the trail of TRP channel structure.
|
| |
J Gen Physiol, 133,
239-244.
|
|
|
|
|
|
|
X.Cheng,
I.Ivanov,
H.Wang,
S.M.Sine,
and
J.A.McCammon
(2009).
Molecular-dynamics simulations of ELIC-a prokaryotic homologue of the nicotinic acetylcholine receptor.
|
| |
Biophys J, 96,
4502-4513.
|
|
|
|
|
|
|
Y.C.Chang,
W.Wu,
J.L.Zhang,
and
Y.Huang
(2009).
Allosteric activation mechanism of the cys-loop receptors.
|
| |
Acta Pharmacol Sin, 30,
663-672.
|
|
|
|
|
|
|
Y.Muroi,
C.M.Theusch,
C.Czajkowski,
and
M.B.Jackson
(2009).
Distinct structural changes in the GABAA receptor elicited by pentobarbital and GABA.
|
| |
Biophys J, 96,
499-509.
|
|
|
|
|
|
|
Y.Sugiyama,
H.Wang,
T.Hikima,
M.Sato,
J.Kuroda,
T.Takahashi,
T.Ishizuka,
and
H.Yawo
(2009).
Photocurrent attenuation by a single polar-to-nonpolar point mutation of channelrhodopsin-2.
|
| |
Photochem Photobiol Sci, 8,
328-336.
|
|
|
|
|
|
|
A.B.Thode,
S.W.Kruse,
J.C.Nix,
and
D.N.Jones
(2008).
The role of multiple hydrogen-bonding groups in specific alcohol binding sites in proteins: insights from structural studies of LUSH.
|
| |
J Mol Biol, 376,
1360-1376.
|
|
|
PDB codes:
|
|
|
|
|
|
|
|
A.K.Hamouda,
D.C.Chiara,
M.P.Blanton,
and
J.B.Cohen
(2008).
Probing the structure of the affinity-purified and lipid-reconstituted torpedo nicotinic acetylcholine receptor.
|
| |
Biochemistry, 47,
12787-12794.
|
|
|
|
|
|
|
A.Lampert,
A.O.O'Reilly,
S.D.Dib-Hajj,
L.Tyrrell,
B.A.Wallace,
and
S.G.Waxman
(2008).
A Pore-blocking Hydrophobic Motif at the Cytoplasmic Aperture of the Closed-state Nav1.7 Channel Is Disrupted by the Erythromelalgia-associated F1449V Mutation.
|
| |
J Biol Chem, 283,
24118-24127.
|
|
|
|
|
|
|
A.N.Dickey,
and
R.Faller
(2008).
Behavioral differences between phosphatidic acid and phosphatidylcholine in the presence of the nicotinic acetylcholine receptor.
|
| |
Biophys J, 95,
5637-5647.
|
|
|
|
|
|
|
A.Taly,
and
J.P.Changeux
(2008).
Functional organization and conformational dynamics of the nicotinic receptor: a plausible structural interpretation of myasthenic mutations.
|
| |
Ann N Y Acad Sci, 1132,
42-52.
|
|
|
|
|
|
|
B.E.Erkkila,
A.V.Sedelnikova,
and
D.S.Weiss
(2008).
Stoichiometric pore mutations of the GABAAR reveal a pattern of hydrogen bonding with picrotoxin.
|
| |
Biophys J, 94,
4299-4306.
|
|
|
|
|
|
|
C.D.Fowler,
M.A.Arends,
and
P.J.Kenny
(2008).
Subtypes of nicotinic acetylcholine receptors in nicotine reward, dependence, and withdrawal: evidence from genetically modified mice.
|
| |
Behav Pharmacol, 19,
461-484.
|
|
|
|
|
|
|
D.H.Chen,
J.Jakana,
X.Liu,
M.F.Schmid,
and
W.Chiu
(2008).
Achievable resolution from images of biological specimens acquired from a 4k x 4k CCD camera in a 300-kV electron cryomicroscope.
|
| |
J Struct Biol, 163,
45-52.
|
|
|
|
|
|
|
E.H.Egelman
(2008).
Problems in fitting high resolution structures into electron microscopic reconstructions.
|
| |
HFSP J, 2,
324-331.
|
|
|
|
|
|
|
E.J.Haddadian,
M.H.Cheng,
R.D.Coalson,
Y.Xu,
and
P.Tang
(2008).
In silico models for the human alpha4beta2 nicotinic acetylcholine receptor.
|
| |
J Phys Chem B, 112,
13981-13990.
|
|
|
|
|
|
|
F.Fornasiero,
H.G.Park,
J.K.Holt,
M.Stadermann,
C.P.Grigoropoulos,
A.Noy,
and
O.Bakajin
(2008).
Ion exclusion by sub-2-nm carbon nanotube pores.
|
| |
Proc Natl Acad Sci U S A, 105,
17250-17255.
|
|
|
|
|
|
|
F.Krieger,
A.Mourot,
R.Araoz,
F.Kotzyba-Hibert,
J.Molgó,
E.Bamberg,
and
M.Goeldner
(2008).
Fluorescent agonists for the Torpedo nicotinic acetylcholine receptor.
|
| |
Chembiochem, 9,
1146-1153.
|
|
|
|
|
|
|
G.A.Asmar-Rovira,
A.M.Asseo-García,
O.Quesada,
M.A.Hanson,
A.Cheng,
C.Nogueras,
J.A.Lasalde-Dominicci,
and
R.C.Stevens
(2008).
Biophysical and ion channel functional characterization of the Torpedo californica nicotinic acetylcholine receptor in varying detergent-lipid environments.
|
| |
J Membr Biol, 223,
13-26.
|
|
|
|
|
|
|
G.A.Fernández Nievas,
F.J.Barrantes,
and
S.S.Antollini
(2008).
Modulation of nicotinic acetylcholine receptor conformational state by free fatty acids and steroids.
|
| |
J Biol Chem, 283,
21478-21486.
|
|
|
|
|
|
|
G.Brannigan,
J.Hénin,
R.Law,
R.Eckenhoff,
and
M.L.Klein
(2008).
Embedded cholesterol in the nicotinic acetylcholine receptor.
|
| |
Proc Natl Acad Sci U S A, 105,
14418-14423.
|
|
|
|
|
|
|
G.D.Cymes,
and
C.Grosman
(2008).
Pore-opening mechanism of the nicotinic acetylcholine receptor evinced by proton transfer.
|
| |
Nat Struct Mol Biol, 15,
389-396.
|
|
|
|
|
|
|
G.T.Young,
R.Zwart,
A.S.Walker,
E.Sher,
and
N.S.Millar
(2008).
Potentiation of alpha7 nicotinic acetylcholine receptors via an allosteric transmembrane site.
|
| |
Proc Natl Acad Sci U S A, 105,
14686-14691.
|
|
|
|
|
|
|
H.L.Wang,
X.Cheng,
P.Taylor,
J.A.McCammon,
and
S.M.Sine
(2008).
Control of cation permeation through the nicotinic receptor channel.
|
| |
PLoS Comput Biol, 4,
e41.
|
|
|
|
|
|
|
H.Yang,
J.Shi,
G.Zhang,
J.Yang,
K.Delaloye,
and
J.Cui
(2008).
Activation of Slo1 BK channels by Mg2+ coordinated between the voltage sensor and RCK1 domains.
|
| |
Nat Struct Mol Biol, 15,
1152-1159.
|
|
|
|
|
|
|
I.A.Lobo,
R.A.Harris,
and
J.R.Trudell
(2008).
Cross-linking of sites involved with alcohol action between transmembrane segments 1 and 3 of the glycine receptor following activation.
|
| |
J Neurochem, 104,
1649-1662.
|
|
|
|
|
|
|
J.Krüger,
and
W.B.Fischer
(2008).
Exploring the conformational space of Vpu from HIV-1: a versatile adaptable protein.
|
| |
J Comput Chem, 29,
2416-2424.
|
|
|
|
|
|
|
J.Payandeh,
C.Li,
M.Ramjeesingh,
E.Poduch,
C.E.Bear,
and
E.F.Pai
(2008).
Probing structure-function relationships and gating mechanisms in the CorA Mg2+ transport system.
|
| |
J Biol Chem, 283,
11721-11733.
|
|
|
|
|
|
|
L.M.Sharkey,
and
C.Czajkowski
(2008).
Individually monitoring ligand-induced changes in the structure of the GABAA receptor at benzodiazepine binding site and non-binding-site interfaces.
|
| |
Mol Pharmacol, 74,
203-212.
|
|
|
|
|
|
|
M.Ellison,
Z.P.Feng,
A.J.Park,
X.Zhang,
B.M.Olivera,
J.M.McIntosh,
and
R.S.Norton
(2008).
Alpha-RgIA, a novel conotoxin that blocks the alpha9alpha10 nAChR: structure and identification of key receptor-binding residues.
|
| |
J Mol Biol, 377,
1216-1227.
|
|
|
PDB codes:
|
|
|
|
|
|
|
|
M.Ihara,
T.Okajima,
A.Yamashita,
T.Oda,
K.Hirata,
H.Nishiwaki,
T.Morimoto,
M.Akamatsu,
Y.Ashikawa,
S.Kuroda,
R.Mega,
S.Kuramitsu,
D.B.Sattelle,
and
K.Matsuda
(2008).
Crystal structures of Lymnaea stagnalis AChBP in complex with neonicotinoid insecticides imidacloprid and clothianidin.
|
| |
Invert Neurosci, 8,
71-81.
|
|
|
PDB codes:
|
|
|
|
|
|
|
|
M.Jansen,
M.Bali,
and
M.H.Akabas
(2008).
Modular Design of Cys-loop Ligand-gated Ion Channels: Functional 5-HT3 and GABA {rho}1 Receptors Lacking the Large Cytoplasmic M3M4 Loop.
|
| |
J Gen Physiol, 131,
137-146.
|
|
|
|
|
|
|
M.L.Tierney,
T.Luu,
and
P.W.Gage
(2008).
Functional asymmetry of the conserved cystine loops in alphabetagamma GABA A receptors revealed by the response to GABA activation and drug potentiation.
|
| |
Int J Biochem Cell Biol, 40,
968-979.
|
|
|
|
|
|
|
M.R.Picciotto,
N.A.Addy,
Y.S.Mineur,
and
D.H.Brunzell
(2008).
It is not "either/or": activation and desensitization of nicotinic acetylcholine receptors both contribute to behaviors related to nicotine addiction and mood.
|
| |
Prog Neurobiol, 84,
329-342.
|
|
|
|
|
|
|
O.Henschel,
K.E.Gipson,
and
A.Bordey
(2008).
GABAA receptors, anesthetics and anticonvulsants in brain development.
|
| |
CNS Neurol Disord Drug Targets, 7,
211-224.
|
|
|
|
|
|
|
P.Qian,
P.A.Bullough,
and
C.N.Hunter
(2008).
Three-dimensional reconstruction of a membrane-bending complex: the RC-LH1-PufX core dimer of Rhodobacter sphaeroides.
|
| |
J Biol Chem, 283,
14002-14011.
|
|
|
|
|
|
|
Q.Liu,
K.W.Yu,
Y.C.Chang,
R.J.Lukas,
and
J.Wu
(2008).
Agonist-induced hump current production in heterologously-expressed human alpha4beta2-nicotinic acetylcholine receptors.
|
| |
Acta Pharmacol Sin, 29,
305-319.
|
|
|
|
|
|
|
R.J.Hilf,
and
R.Dutzler
(2008).
X-ray structure of a prokaryotic pentameric ligand-gated ion channel.
|
| |
Nature, 452,
375-379.
|
|
|
PDB code:
|
|
|
|
|
|
|
|
R.M.Glaeser
(2008).
Macromolecular structures without crystals.
|
| |
Proc Natl Acad Sci U S A, 105,
1779-1780.
|
|
|
|
|
|
|
R.W.Olsen,
and
W.Sieghart
(2008).
International Union of Pharmacology. LXX. Subtypes of gamma-aminobutyric acid(A) receptors: classification on the basis of subunit composition, pharmacology, and function. Update.
|
| |
Pharmacol Rev, 60,
243-260.
|
|
|
|
|
|
|
S.A.Pless,
and
J.W.Lynch
(2008).
Illuminating the structure and function of Cys-loop receptors.
|
| |
Clin Exp Pharmacol Physiol, 35,
1137-1142.
|
|
|
|
|
|
|
S.A.Pless,
K.S.Millen,
A.P.Hanek,
J.W.Lynch,
H.A.Lester,
S.C.Lummis,
and
D.A.Dougherty
(2008).
A cation-pi interaction in the binding site of the glycine receptor is mediated by a phenylalanine residue.
|
| |
J Neurosci, 28,
10937-10942.
|
|
|
|
|
|
|
S.B.Hansen,
H.L.Wang,
P.Taylor,
and
S.M.Sine
(2008).
An Ion Selectivity Filter in the Extracellular Domain of Cys-loop Receptors Reveals Determinants for Ion Conductance.
|
| |
J Biol Chem, 283,
36066-36070.
|
|
|
PDB code:
|
|
|
|
|
|
|
|
S.Nirthanan,
G.Garcia,
D.C.Chiara,
S.S.Husain,
and
J.B.Cohen
(2008).
Identification of binding sites in the nicotinic acetylcholine receptor for TDBzl-etomidate, a photoreactive positive allosteric effector.
|
| |
J Biol Chem, 283,
22051-22062.
|
|
|
|
|
|
|
T.Nagata,
S.Iizumi,
K.Satoh,
and
S.Kikuchi
(2008).
Comparative molecular biological analysis of membrane transport genes in organisms.
|
| |
Plant Mol Biol, 66,
565-585.
|
|
|
|
|
|
|
V.Bondarenko,
V.E.Yushmanov,
Y.Xu,
and
P.Tang
(2008).
NMR study of general anesthetic interaction with nAChR beta2 subunit.
|
| |
Biophys J, 94,
1681-1688.
|
|
|
|
|
|
|
V.Vásquez,
M.Sotomayor,
D.M.Cortes,
B.Roux,
K.Schulten,
and
E.Perozo
(2008).
Three-dimensional architecture of membrane-embedded MscS in the closed conformation.
|
| |
J Mol Biol, 378,
55-70.
|
|
|
|
|
|
|
W.H.Bisson,
G.Westera,
P.A.Schubiger,
and
L.Scapozza
(2008).
Homology modeling and dynamics of the extracellular domain of rat and human neuronal nicotinic acetylcholine receptor subtypes alpha4beta2 and alpha7.
|
| |
J Mol Model, 14,
891-899.
|
|
|
|
|
|
|
W.Wang,
S.S.Black,
M.D.Edwards,
S.Miller,
E.L.Morrison,
W.Bartlett,
C.Dong,
J.H.Naismith,
and
I.R.Booth
(2008).
The structure of an open form of an E. coli mechanosensitive channel at 3.45 A resolution.
|
| |
Science, 321,
1179-1183.
|
|
|
PDB code:
|
|
|
|
|
|
|
|
W.Y.Lee,
C.R.Free,
and
S.M.Sine
(2008).
Nicotinic receptor interloop proline anchors beta1-beta2 and Cys loops in coupling agonist binding to channel gating.
|
| |
J Gen Physiol, 132,
265-278.
|
|
|
|
|
|
|
X.Liu,
Y.Xu,
H.Li,
X.Wang,
H.Jiang,
and
F.J.Barrantes
(2008).
Mechanics of channel gating of the nicotinic acetylcholine receptor.
|
| |
PLoS Comput Biol, 4,
e19.
|
|
|
|
|
|
|
X.Q.Hu,
and
D.M.Lovinger
(2008).
The L293 residue in transmembrane domain 2 of the 5-HT3A receptor is a molecular determinant of allosteric modulation by 5-hydroxyindole.
|
| |
Neuropharmacology, 54,
1153-1165.
|
|
|
|
|
|
|
Y.Fujiyoshi,
and
N.Unwin
(2008).
Electron crystallography of proteins in membranes.
|
| |
Curr Opin Struct Biol, 18,
587-592.
|
|
|
|
|
|
|
Y.Gohon,
T.Dahmane,
R.W.Ruigrok,
P.Schuck,
D.Charvolin,
F.Rappaport,
P.Timmins,
D.M.Engelman,
C.Tribet,
J.L.Popot,
and
C.Ebel
(2008).
Bacteriorhodopsin/amphipol complexes: structural and functional properties.
|
| |
Biophys J, 94,
3523-3537.
|
|
|
|
|
|
|
Y.Y.Kim,
D.Y.Kim,
D.Shim,
W.Y.Song,
J.Lee,
J.I.Schroeder,
S.Kim,
N.Moran,
and
Y.Lee
(2008).
Expression of the novel wheat gene TM20 confers enhanced cadmium tolerance to bakers' yeast.
|
| |
J Biol Chem, 283,
15893-15902.
|
|
|
|
|
|
|
A.J.Thompson,
and
S.C.Lummis
(2007).
The 5-HT3 receptor as a therapeutic target.
|
| |
Expert Opin Ther Targets, 11,
527-540.
|
|
|
|
|
|
|
A.Jha,
D.J.Cadugan,
P.Purohit,
and
A.Auerbach
(2007).
Acetylcholine receptor gating at extracellular transmembrane domain interface: the cys-loop and M2-M3 linker.
|
| |
J Gen Physiol, 130,
547-558.
|
|
|
|
|
|
|
A.K.Hamouda,
M.Sanghvi,
D.C.Chiara,
J.B.Cohen,
and
M.P.Blanton
(2007).
Identifying the lipid-protein interface of the alpha4beta2 neuronal nicotinic acetylcholine receptor: hydrophobic photolabeling studies with 3-(trifluoromethyl)-3-(m-[125I]iodophenyl)diazirine.
|
| |
Biochemistry, 46,
13837-13846.
|
|
|
|
|
|
|
A.M.Hosie,
M.E.Wilkins,
and
T.G.Smart
(2007).
Neurosteroid binding sites on GABA(A) receptors.
|
| |
Pharmacol Ther, 116,
7.
|
|
|
|
|
|
|
A.Oshima,
K.Tani,
Y.Hiroaki,
Y.Fujiyoshi,
and
G.E.Sosinsky
(2007).
Three-dimensional structure of a human connexin26 gap junction channel reveals a plug in the vestibule.
|
| |
Proc Natl Acad Sci U S A, 104,
10034-10039.
|
|
|
|
|
|
|
A.Szarecka,
Y.Xu,
and
P.Tang
(2007).
Dynamics of heteropentameric nicotinic acetylcholine receptor: implications of the gating mechanism.
|
| |
Proteins, 68,
948-960.
|
|
|
|
|
|
|
A.Taly
(2007).
Opened by a twist: a gating mechanism for the nicotinic acetylcholine receptor.
|
| |
Eur Biophys J, 36,
911-918.
|
|
|
|
|
|
|
A.V.Kalueff
(2007).
Mapping convulsants' binding to the GABA-A receptor chloride ionophore: a proposed model for channel binding sites.
|
| |
Neurochem Int, 50,
61-68.
|
|
|
|
|
|
|
B.E.McKay,
A.N.Placzek,
and
J.A.Dani
(2007).
Regulation of synaptic transmission and plasticity by neuronal nicotinic acetylcholine receptors.
|
| |
Biochem Pharmacol, 74,
1120-1133.
|
|
|
|
|
|
|
C.D.Dellisanti,
Y.Yao,
J.C.Stroud,
Z.Z.Wang,
and
L.Chen
(2007).
Crystal structure of the extracellular domain of nAChR alpha1 bound to alpha-bungarotoxin at 1.94 A resolution.
|
| |
Nat Neurosci, 10,
953-962.
|
|
|
PDB code:
|
|
|
|
|
|
|
|
C.O.Sorzano,
S.Jonic,
M.Cottevieille,
E.Larquet,
N.Boisset,
and
S.Marco
(2007).
3D electron microscopy of biological nanomachines: principles and applications.
|
| |
Eur Biophys J, 36,
995.
|
|
|
|
|
|
|
D.J.Cadugan,
and
A.Auerbach
(2007).
Conformational dynamics of the alphaM3 transmembrane helix during acetylcholine receptor channel gating.
|
| |
Biophys J, 93,
859-865.
|
|
|
|
|
|
|
D.Kalamida,
K.Poulas,
V.Avramopoulou,
E.Fostieri,
G.Lagoumintzis,
K.Lazaridis,
A.Sideri,
M.Zouridakis,
and
S.J.Tzartos
(2007).
Muscle and neuronal nicotinic acetylcholine receptors. Structure, function and pathogenicity.
|
| |
FEBS J, 274,
3799-3845.
|
|
|
|
|
|
|
D.L.Minor
(2007).
The neurobiologist's guide to structural biology: a primer on why macromolecular structure matters and how to evaluate structural data.
|
| |
Neuron, 54,
511-533.
|
|
|
|
|
|
|
D.S.Anderson,
P.Adhikari,
K.D.Weaver,
A.L.Crumbliss,
and
T.A.Mietzner
(2007).
The Haemophilus influenzae hFbpABC Fe3+ transporter: analysis of the membrane permease and development of a gallium-based screen for mutants.
|
| |
J Bacteriol, 189,
5130-5141.
|
|
|
|
|
|
|
E.H.Egelman
(2007).
Single-particle reconstruction from EM images of helical filaments.
|
| |
Curr Opin Struct Biol, 17,
556-561.
|
|
|
|
|
|
|
E.J.Bertaccini,
J.R.Trudell,
and
E.Lindahl
(2007).
Normal-mode analysis of the glycine alpha1 receptor by three separate methods.
|
| |
J Chem Inf Model, 47,
1572-1579.
|
|
|
|
|
|
|
G.Frugier,
F.Coussen,
M.F.Giraud,
M.F.Odessa,
M.B.Emerit,
E.Boué-Grabot,
and
M.Garret
(2007).
A gamma 2(R43Q) mutation, linked to epilepsy in humans, alters GABAA receptor assembly and modifies subunit composition on the cell surface.
|
| |
J Biol Chem, 282,
3819-3828.
|
|
|
|
|
|
|
G.von Heijne
(2007).
The membrane protein universe: what's out there and why bother?
|
| |
J Intern Med, 261,
543-557.
|
|
|
|
|
|
|
H.Lu,
X.Chen,
and
C.G.Zhan
(2007).
First-principles calculation of pKa for cocaine, nicotine, neurotransmitters, and anilines in aqueous solution.
|
| |
J Phys Chem B, 111,
10599-10605.
|
|
|
|
|
|
|
H.Shen,
Q.H.Gong,
C.Aoki,
M.Yuan,
Y.Ruderman,
M.Dattilo,
K.Williams,
and
S.S.Smith
(2007).
Reversal of neurosteroid effects at alpha4beta2delta GABAA receptors triggers anxiety at puberty.
|
| |
Nat Neurosci, 10,
469-477.
|
|
|
|
|
|
|
J.D.Otero-Cruz,
C.A.Báez-Pagán,
I.M.Caraballo-González,
and
J.A.Lasalde-Dominicci
(2007).
Tryptophan-scanning mutagenesis in the alphaM3 transmembrane domain of the muscle-type acetylcholine receptor. A spring model revealed.
|
| |
J Biol Chem, 282,
9162-9171.
|
|
|
|
|
|
|
J.K.Foskett,
C.White,
K.H.Cheung,
and
D.O.Mak
(2007).
Inositol trisphosphate receptor Ca2+ release channels.
|
| |
Physiol Rev, 87,
593-658.
|
|
|
|
|
|
|
J.Li,
X.Gong,
H.Lu,
D.Li,
H.Fang,
and
R.Zhou
(2007).
Electrostatic gating of a nanometer water channel.
|
| |
Proc Natl Acad Sci U S A, 104,
3687-3692.
|
|
|
|
|
|
|
J.Wang,
H.A.Lester,
and
D.A.Dougherty
(2007).
Establishing an ion pair interaction in the homomeric rho1 gamma-aminobutyric acid type A receptor that contributes to the gating pathway.
|
| |
J Biol Chem, 282,
26210-26216.
|
|
|
|
|
|
|
K.Speranskiy,
M.Cascio,
and
M.Kurnikova
(2007).
Homology modeling and molecular dynamics simulations of the glycine receptor ligand binding domain.
|
| |
Proteins, 67,
950-960.
|
|
|
|
|
|
|
M.Bali,
and
M.H.Akabas
(2007).
The location of a closed channel gate in the GABAA receptor channel.
|
| |
J Gen Physiol, 129,
145-159.
|
|
|
|
|
|
|
M.H.Cheng,
M.Cascio,
and
R.D.Coalson
(2007).
Homology modeling and molecular dynamics simulations of the alpha1 glycine receptor reveals different states of the channel.
|
| |
Proteins, 68,
581-593.
|
|
|
|
|
|
|
M.Ihara,
M.Shimomura,
C.Ishida,
H.Nishiwaki,
M.Akamatsu,
D.B.Sattelle,
and
K.Matsuda
(2007).
A hypothesis to account for the selective and diverse actions of neonicotinoid insecticides at their molecular targets, nicotinic acetylcholine receptors: catch and release in hydrogen bond networks.
|
| |
Invert Neurosci, 7,
47-51.
|
|
|
|
|
|
|
M.J.Gallagher,
L.Ding,
A.Maheshwari,
and
R.L.Macdonald
(2007).
The GABAA receptor alpha1 subunit epilepsy mutation A322D inhibits transmembrane helix formation and causes proteasomal degradation.
|
| |
Proc Natl Acad Sci U S A, 104,
12999-13004.
|
|
|
|
|
|
|
M.N.Romanelli,
P.Gratteri,
L.Guandalini,
E.Martini,
C.Bonaccini,
and
F.Gualtieri
(2007).
Central Nicotinic Receptors: Structure, Function, Ligands, and Therapeutic Potential.
|
| |
ChemMedChem, 2,
746-767.
|
|
|
|
|
|
|
M.S.Alam,
J.Huang,
F.Ozoe,
F.Matsumura,
and
Y.Ozoe
(2007).
Synthesis, 3D-QSAR, and docking studies of 1-phenyl-1H-1,2,3-triazoles as selective antagonists for beta3 over alpha1beta2gamma2 GABA receptors.
|
| |
Bioorg Med Chem, 15,
5090-5104.
|
|
|
|
|
|
|
N.Bocquet,
L.Prado de Carvalho,
J.Cartaud,
J.Neyton,
C.Le Poupon,
A.Taly,
T.Grutter,
J.P.Changeux,
and
P.J.Corringer
(2007).
A prokaryotic proton-gated ion channel from the nicotinic acetylcholine receptor family.
|
| |
Nature, 445,
116-119.
|
|
|
|
|
|
|
P.Purohit,
A.Mitra,
and
A.Auerbach
(2007).
A stepwise mechanism for acetylcholine receptor channel gating.
|
| |
Nature, 446,
930-933.
|
|
|
|
|
|
|
P.T.Williamson,
A.Verhoeven,
K.W.Miller,
B.H.Meier,
and
A.Watts
(2007).
The conformation of acetylcholine at its target site in the membrane-embedded nicotinic acetylcholine receptor.
|
| |
Proc Natl Acad Sci U S A, 104,
18031-18036.
|
|
|
|
|
|
|
R.K.Hite,
S.Raunser,
and
T.Walz
(2007).
Revival of electron crystallography.
|
| |
Curr Opin Struct Biol, 17,
389-395.
|
|
|
|
|
|
|
R.Moaddel,
K.Jozwiak,
and
I.W.Wainer
(2007).
Allosteric modifiers of neuronal nicotinic acetylcholine receptors: new methods, new opportunities.
|
| |
Med Res Rev, 27,
723-753.
|
|
|
|
|
|
|
S.B.Hansen,
and
P.Taylor
(2007).
Galanthamine and non-competitive inhibitor binding to ACh-binding protein: evidence for a binding site on non-alpha-subunit interfaces of heteromeric neuronal nicotinic receptors.
|
| |
J Mol Biol, 369,
895-901.
|
|
|
PDB codes:
|
|
|
|
|
|
|
|
S.Futaki,
and
K.Asami
(2007).
Ligand-induced extramembrane conformation switch controlling alamethicin assembly and the channel current.
|
| |
Chem Biodivers, 4,
1313-1322.
|
|
|
|
|
|
|
S.M.Saparov,
K.Erlandson,
K.Cannon,
J.Schaletzky,
S.Schulman,
T.A.Rapoport,
and
P.Pohl
(2007).
Determining the conductance of the SecY protein translocation channel for small molecules.
|
| |
Mol Cell, 26,
501-509.
|
|
|
|
|
|
|
S.S.Ericksen,
and
A.J.Boileau
(2007).
Tandem couture: Cys-loop receptor concatamer insights and caveats.
|
| |
Mol Neurobiol, 35,
113-128.
|
|
|
|
|
|
|
T.Huyton,
J.Rossjohn,
and
M.Wilce
(2007).
Toll-like receptors: structural pieces of a curve-shaped puzzle.
|
| |
Immunol Cell Biol, 85,
406-410.
|
|
|
|
|
|
|
T.K.Sixma
(2007).
Nicotinic receptor structure emerging slowly.
|
| |
Nat Neurosci, 10,
937-938.
|
|
|
|
|
|
|
V.Bondarenko,
Y.Xu,
and
P.Tang
(2007).
Structure of the first transmembrane domain of the neuronal acetylcholine receptor beta2 subunit.
|
| |
Biophys J, 92,
1616-1622.
|
|
|
PDB code:
|
|
|
|
|
|
|
|
V.Campagna-Slater,
and
D.F.Weaver
(2007).
Anaesthetic binding sites for etomidate and propofol on a GABAA receptor model.
|
| |
Neurosci Lett, 418,
28-33.
|
|
|
|
|
|
|
V.N.Uversky
(2007).
Nanoimaging in protein-misfolding and -conformational diseases.
|
| |
Nanomed, 2,
615-643.
|
|
|
|
|
|
|
W.J.de Jonge,
and
L.Ulloa
(2007).
The alpha7 nicotinic acetylcholine receptor as a pharmacological target for inflammation.
|
| |
Br J Pharmacol, 151,
915-929.
|
|
|
|
|
|
|
X.Cheng,
I.Ivanov,
H.Wang,
S.M.Sine,
and
J.A.McCammon
(2007).
Nanosecond-timescale conformational dynamics of the human alpha7 nicotinic acetylcholine receptor.
|
| |
Biophys J, 93,
2622-2634.
|
|
|
|
|
|
|
X.Yan,
K.A.Dryden,
J.Tang,
and
T.S.Baker
(2007).
Ab initio random model method facilitates 3D reconstruction of icosahedral particles.
|
| |
J Struct Biol, 157,
211-225.
|
|
|
|
|
|
|
X.Zeng,
B.Gipson,
Z.Y.Zheng,
L.Renault,
and
H.Stahlberg
(2007).
Automatic lattice determination for two-dimensional crystal images.
|
| |
J Struct Biol, 160,
353-361.
|
|
|
|
|
|
|
Y.Cheng,
J.K.Suen,
Z.Radić,
S.D.Bond,
M.J.Holst,
and
J.A.McCammon
(2007).
Continuum simulations of acetylcholine diffusion with reaction-determined boundaries in neuromuscular junction models.
|
| |
Biophys Chem, 127,
129-139.
|
|
|
|
|
|
|
Y.Tsai,
M.R.Sawaya,
G.C.Cannon,
F.Cai,
E.B.Williams,
S.Heinhorst,
C.A.Kerfeld,
and
T.O.Yeates
(2007).
Structural analysis of CsoS1A and the protein shell of the Halothiobacillus neapolitanus carboxysome.
|
| |
PLoS Biol, 5,
e144.
|
|
|
PDB codes:
|
|
|
|
|
|
|
|
Z.Yang,
B.A.Cromer,
R.J.Harvey,
M.W.Parker,
and
J.W.Lynch
(2007).
A proposed structural basis for picrotoxinin and picrotin binding in the glycine receptor pore.
|
| |
J Neurochem, 103,
580-589.
|
|
|
|
|
|
|
A.K.Hamouda,
D.C.Chiara,
D.Sauls,
J.B.Cohen,
and
M.P.Blanton
(2006).
Cholesterol interacts with transmembrane alpha-helices M1, M3, and M4 of the Torpedo nicotinic acetylcholine receptor: photolabeling studies using [3H]Azicholesterol.
|
| |
Biochemistry, 45,
976-986.
|
|
|
|
|
|
|
A.K.Hamouda,
M.Sanghvi,
D.Sauls,
T.K.Machu,
and
M.P.Blanton
(2006).
Assessing the lipid requirements of the Torpedo californica nicotinic acetylcholine receptor.
|
| |
Biochemistry, 45,
4327-4337.
|
|
|
|
|
|
|
A.Kapur,
M.Davies,
W.F.Dryden,
and
S.M.Dunn
(2006).
Activation of the Torpedo nicotinic acetylcholine receptor. The contribution of residues alphaArg55 and gammaGlu93.
|
| |
FEBS J, 273,
960-970.
|
|
|
|
|
|
|
A.M.Hosie,
M.E.Wilkins,
H.M.da Silva,
and
T.G.Smart
(2006).
Endogenous neurosteroids regulate GABAA receptors through two discrete transmembrane sites.
|
| |
Nature, 444,
486-489.
|
|
|
|
|
|
|
A.Mourot,
T.Grutter,
M.Goeldner,
and
F.Kotzyba-Hibert
(2006).
Dynamic structural investigations on the torpedo nicotinic acetylcholine receptor by time-resolved photoaffinity labeling.
|
| |
Chembiochem, 7,
570-583.
|
|
|
|
|
|
|
A.P.Yeh,
A.McMillan,
and
M.H.Stowell
(2006).
Rapid and simple protein-stability screens: application to membrane proteins.
|
| |
Acta Crystallogr D Biol Crystallogr, 62,
451-457.
|
|
|
|
|
|
|
A.Sedelnikova,
B.E.Erkkila,
H.Harris,
S.O.Zakharkin,
and
D.S.Weiss
(2006).
Stoichiometry of a pore mutation that abolishes picrotoxin-mediated antagonism of the GABAA receptor.
|
| |
J Physiol, 577,
569-577.
|
|
|
|
|
|
|
B.Corry
(2006).
An energy-efficient gating mechanism in the acetylcholine receptor channel suggested by molecular and Brownian dynamics.
|
| |
Biophys J, 90,
799-810.
|
|
|
|
|
|
|
B.Corry
(2006).
Understanding ion channel selectivity and gating and their role in cellular signalling.
|
| |
Mol Biosyst, 2,
527-535.
|
|
|
|
|
|
|
B.Iorga,
D.Herlem,
E.Barré,
and
C.Guillou
(2006).
Acetylcholine nicotinic receptors: finding the putative binding site of allosteric modulators using the "blind docking" approach.
|
| |
J Mol Model, 12,
366-372.
|
|
|
|
|
|
|
B.L.Jones,
P.J.Whiting,
and
L.P.Henderson
(2006).
Mechanisms of anabolic androgenic steroid inhibition of mammalian epsilon-subunit-containing GABAA receptors.
|
| |
J Physiol, 573,
571-593.
|
|
|
|
|
|
|
B.Lang,
and
N.Willcox
(2006).
Autoantibodies in neuromuscular autoimmune disorders.
|
| |
Expert Rev Clin Immunol, 2,
293-307.
|
|
|
|
|
|
|
B.S.Khakh,
and
R.A.North
(2006).
P2X receptors as cell-surface ATP sensors in health and disease.
|
| |
Nature, 442,
527-532.
|
|
|
|
|
|
|
B.Wardell,
P.S.Marik,
D.Piper,
T.Rutar,
E.M.Jorgensen,
and
B.A.Bamber
(2006).
Residues in the first transmembrane domain of the Caenorhabditis elegans GABA(A) receptor confer sensitivity to the neurosteroid pregnenolone sulfate.
|
| |
Br J Pharmacol, 148,
162-172.
|
|
|
|
|
|
|
C.Campo-Soria,
Y.Chang,
and
D.S.Weiss
(2006).
Mechanism of action of benzodiazepines on GABAA receptors.
|
| |
Br J Pharmacol, 148,
984-990.
|
|
|
|
|
|
|
C.G.Kim,
V.Lemaitre,
A.Watts,
and
W.B.Fischer
(2006).
Drug-protein interaction with Vpu from HIV-1: proposing binding sites for amiloride and one of its derivatives.
|
| |
Anal Bioanal Chem, 386,
2213-2217.
|
|
|
|
|
|
|
D.Colquhoun
(2006).
Agonist-activated ion channels.
|
| |
Br J Pharmacol, 147,
S17-S26.
|
|
|
|
|
|
|
D.G.Hill,
and
J.E.Baenziger
(2006).
The net orientation of nicotinic receptor transmembrane alpha-helices in the resting and desensitized states.
|
| |
Biophys J, 91,
705-714.
|
|
|
|
|
|
|
D.L.Davies,
L.Asatryan,
S.T.Kuo,
J.J.Woodward,
B.F.King,
R.L.Alkana,
C.Xiao,
J.H.Ye,
H.Sun,
L.Zhang,
X.Q.Hu,
V.Hayrapetyan,
D.M.Lovinger,
and
T.K.Machu
(2006).
Effects of ethanol on adenosine 5'-triphosphate-gated purinergic and 5-hydroxytryptamine receptors.
|
| |
Alcohol Clin Exp Res, 30,
349-358.
|
|
|
|
|
|
|
D.V.Mamaev,
D.A.Aliverdieva,
D.I.Bondarenko,
and
K.F.Sholtz
(2006).
Study of active site topography of rat liver mitochondrial dicarboxylate transporter using lipophilic substrate derivatives.
|
| |
Biochemistry (Mosc), 71,
800-809.
|
|
|
|
|
|
|
D.Zhang,
J.Gullingsrud,
and
J.A.McCammon
(2006).
Potentials of mean force for acetylcholine unbinding from the alpha7 nicotinic acetylcholine receptor ligand-binding domain.
|
| |
J Am Chem Soc, 128,
3019-3026.
|
|
|
|
|
|
|
E.O.Oloo,
C.Kandt,
M.L.O'Mara,
and
D.P.Tieleman
(2006).
Computer simulations of ABC transporter components.
|
| |
Biochem Cell Biol, 84,
900-911.
|
|
|
|
|
|
|
G.E.Yevenes,
G.Moraga-Cid,
L.Guzmán,
S.Haeger,
L.Oliveira,
J.Olate,
G.Schmalzing,
and
L.G.Aguayo
(2006).
Molecular determinants for G protein betagamma modulation of ionotropic glycine receptors.
|
| |
J Biol Chem, 281,
39300-39307.
|
|
|
|
|
|
|
G.R.Guzmán,
A.Ortiz-Acevedo,
A.Ricardo,
L.V.Rojas,
and
J.A.Lasalde-Dominicci
(2006).
The polarity of lipid-exposed residues contributes to the functional differences between Torpedo and muscle-type nicotinic receptors.
|
| |
J Membr Biol, 214,
131-138.
|
|
|
|
|
|
|
H.Allain,
A.Hervé,
and
D.Bentué-Ferrer
(2006).
The anti-dementia drugs: myth, hype or reality?
|
| |
Clin Neuropharmacol, 29,
10-14.
|
|
|
|
|
|
|
H.Betz,
and
B.Laube
(2006).
Glycine receptors: recent insights into their structural organization and functional diversity.
|
| |
J Neurochem, 97,
1600-1610.
|
|
|
|
|
|
|
H.R.Arias,
P.Bhumireddy,
and
C.Bouzat
(2006).
Molecular mechanisms and binding site locations for noncompetitive antagonists of nicotinic acetylcholine receptors.
|
| |
Int J Biochem Cell Biol, 38,
1254-1276.
|
|
|
|
|
|
|
J.Grandl,
C.Danelon,
R.Hovius,
and
H.Vogel
(2006).
Functional asymmetry of transmembrane segments in nicotinic acetylcholine receptors.
|
| |
Eur Biophys J, 35,
685-693.
|
|
|
|
|
|
|
K.Hedfalk,
S.Törnroth-Horsefield,
M.Nyblom,
U.Johanson,
P.Kjellbom,
and
R.Neutze
(2006).
Aquaporin gating.
|
| |
Curr Opin Struct Biol, 16,
447-456.
|
|
|
|
| | |
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