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131 a.a.
Key reference
DOI no: 10.1016/S0945-053X(02)00119-1 Matrix Biol 22:145-152 (2003) PubMed id: 12782141
Crystal structure of the collagen alpha1(VIII) NC1 trimer. M.Kvansakul, O.Bogin, E.Hohenester, A.Yayon. ABSTRACT
Collagen VIII is a major component of Descemet's membrane and is also found in vascular subendothelial matrices. The C-terminal non-collagenous domain (NC1) domain of collagen VIII, which is a member of the C1q-like protein family, forms a stable trimer and is thought to direct the assembly of the collagen triple helix, as well as polygonal supramolecular structures. We have solved the crystal structure of the mouse alpha1(VIII)(3) NC1 domain trimer at 1.9 A resolution. Each subunit of the intimate NC1 trimer consists of a ten-stranded beta-sandwich. The surface of the collagen VIII NC1 trimer presents three strips of partially exposed aromatic residues shown to interact with the non-ionic detergent CHAPS, which are likely to be involved in supramolecular assemblies. Equivalent strips exist in the NC1 domain of the closely related collagen X, suggesting a conserved assembly mechanism. Surprisingly, the collagen VIII NC1 trimer lacks the buried calcium cluster of the collagen X NC1 trimer. The mouse alpha1(VIII) and alpha2(VIII) NC1 domains are 71.5% identical in sequence, with the differences being concentrated on the NC1 trimer surface. A few non-conservative substitutions map to the subunit interfaces near the surface, but it is not obvious from the structure to what extent they determine the preferred assembly of collagen VIII alpha1 and alpha2 chains into homotrimers.
Literature references that cite this PDB file's key reference
PubMed id Reference
19023665 G.Verdone, A.Corazza, S.A.Colebrooke, D.Cicero, T.Eliseo, J.Boyd, R.Doliana, F.Fogolari, P.Viglino, A.Colombatti, I.D.Campbell, and G.Esposito (2009).
NMR-based homology model for the solution structure of the C-terminal globular domain of EMILIN1.J Biomol NMR, 43, 79-96.
PDB code: 2ka3
18463100 G.Verdone, R.Doliana, A.Corazza, S.A.Colebrooke, P.Spessotto, S.Bot, F.Bucciotti, A.Capuano, A.Silvestri, P.Viglino, I.D.Campbell, A.Colombatti, and G.Esposito (2008).
The solution structure of EMILIN1 globular C1q domain reveals a disordered insertion necessary for interaction with the alpha4beta1 integrin.J Biol Chem, 283, 18947-18956.
19035365 J.M.Gansner, and J.D.Gitlin (2008).
Essential role for the alpha 1 chain of type VIII collagen in zebrafish notochord formation.Dev Dyn, 237, 3715-3726.
17082192 J.Khoshnoodi, J.P.Cartailler, K.Alvares, A.Veis, and B.G.Hudson (2006).
Molecular recognition in the assembly of collagens: terminal noncollagenous domains are key recognition modules in the formation of triple helical protomers.J Biol Chem, 281, 38117-38121.
16135095 D.Bao, Z.Pang, and J.I.Morgan (2005).
The structure and proteolytic processing of Cbln1 complexes.J Neurochem, 95, 618-629.
16249180 S.Réty, S.Salamitou, I.Garcia-Verdugo, D.J.Hulmes, F.Le Hégarat, R.Chaby, and A.Lewit-Bentley (2005).
The crystal structure of the Bacillus anthracis spore surface protein BclA shows remarkable similarity to mammalian proteins.J Biol Chem, 280, 43073-43078.
PDB code: 1wck
15735346 S.Salamitou, S.Réty, F.Le Hégarat, G.Leblon, and A.Lewit-Bentley (2005).
The use of high halide-ion concentrations and automated phasing procedures for the structural analysis of BclA, the major component of the exosporium of Bacillus anthracis spores.Acta Crystallogr D Biol Crystallogr, 61, 344-349.
14990571 S.Stephan, M.J.Sherratt, N.Hodson, C.A.Shuttleworth, and C.M.Kielty (2004).
Expression and supramolecular assembly of recombinant alpha1(viii) and alpha2(viii) collagen homotrimers.J Biol Chem, 279, 21469-21477. The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.
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