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PDBsum entry 1ni0

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protein Protein-protein interface(s) links
Hydrolase PDB id
1ni0
Jmol
Contents
Protein chains
157 a.a.
Waters ×187
PDB id:
1ni0
Name: Hydrolase
Title: Structure of the y94f mutant of the restriction endonuclease pvuii
Structure: Type ii restriction enzyme pvuii. Chain: a, b, c. Synonym: endonuclease pvuii, r.Pvuii. Engineered: yes. Mutation: yes
Source: Proteus vulgaris. Organism_taxid: 585. Gene: pvuiir. Expressed in: escherichia coli. Expression_system_taxid: 562.
Biol. unit: Dimer (from PDB file)
Resolution:
2.50Å     R-factor:   0.228     R-free:   0.290
Authors: P.A.Tucker,M.Kokkinidis,S.Nicolaki,D.Kotsifaki
Key ref: P.A.Tucker et al. Structure of the y94f mutant of the restriction endonuclease pvuii. To be published, .
Date:
20-Dec-02     Release date:   04-Feb-03    
PROCHECK
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 Headers
 References

Protein chains
Pfam   ArchSchema ?
P23657  (T2P2_PROHU) -  Type-2 restriction enzyme PvuII
Seq:
Struc:
157 a.a.
157 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Enzyme reactions 
   Enzyme class: E.C.3.1.21.4  - Type Ii site-specific deoxyribonuclease.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.
      Cofactor: Mg(2+)
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     nucleic acid phosphodiester bond hydrolysis   3 terms 
  Biochemical function     hydrolase activity     5 terms