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Immune system PDB id
1n1f
Jmol
Contents
Protein chain
153 a.a. *
Ligands
NAG
Waters ×164
* Residue conservation analysis
PDB id:
1n1f
Name: Immune system
Title: Crystal structure of human interleukin-19
Structure: Interleukin-19. Chain: a. Synonym: il-19, melanoma differentiation associated protein protein, ng.1. Engineered: yes
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: drosophila melanogaster. Expression_system_taxid: 7227. Expression_system_cell_line: schneider-2 (s2).
Resolution:
1.95Å     R-factor:   0.156     R-free:   0.243
Authors: C.Chang,E.Magracheva,S.Kozlov,S.Fong,G.Tobin,S.Kotenko,A.Wlo A.Zdanov
Key ref:
C.Chang et al. (2003). Crystal structure of interleukin-19 defines a new subfamily of helical cytokines. J Biol Chem, 278, 3308-3313. PubMed id: 12403790 DOI: 10.1074/jbc.M208602200
Date:
17-Oct-02     Release date:   04-Feb-03    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
Q9UHD0  (IL19_HUMAN) -  Interleukin-19
Seq:
Struc: 		177 a.a.
153 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 

 
DOI no: 10.1074/jbc.M208602200 J Biol Chem 278:3308-3313 (2003)
PubMed id: 12403790  
 
 
Crystal structure of interleukin-19 defines a new subfamily of helical cytokines.
C.Chang, E.Magracheva, S.Kozlov, S.Fong, G.Tobin, S.Kotenko, A.Wlodawer, A.Zdanov.
 
  ABSTRACT  
 
Interleukin-19 (IL-19) is a novel cytokine that was initially identified during a sequence data base search aimed at finding potential IL-10 homologs. IL-19 shares a receptor complex with IL-20, indicating that the biological activities of these two cytokines overlap and that both may play an important role in regulating development and proper functioning of the skin. We determined the crystal structure of human recombinant IL-19 and refined it at 1.95-A resolution to an R-factor of 0.157. Unlike IL-10, which forms an intercalated dimer, the molecule of IL-19 is a monomer made of seven amphipathic helices, A-G, creating a unique helical bundle. On the basis of the observed structure, we propose that IL-19, IL-20, and other putative members of the proposed IL-10 family together form a distinct subfamily of helical cytokines.
 
  Selected figure(s)  
 
Figure 1.
Fig. 1. Crystal structure of IL-19. a, stereo tracing of the C atoms of IL-19. The backbone is shown in green, the disulfide bridges are shown in yellow, and the helices are labeled as A-G. b, stereo ribbon representation of IL-19. 		Figure 4.
Fig. 4. Stereo diagram of the superposition of IL-19 with a single domain of IL-10. Ribbons are cyan, coils are brown, and -strands are green. IL-10 is represented in pink, and disulfide bonds are shown in yellow.
 
  The above figures are reprinted by permission from the ASBMB: J Biol Chem (2003, 278, 3308-3313) copyright 2003.  
  Figures were selected by the author.  

Literature references that cite this PDB file's key reference

  PubMed id Reference
21377040 M.Akdis, S.Burgler, R.Crameri, T.Eiwegger, H.Fujita, E.Gomez, S.Klunker, N.Meyer, L.O'Mahony, O.Palomares, C.Rhyner, N.Quaked, A.Schaffartzik, W.Van De Veen, S.Zeller, M.Zimmermann, and C.A.Akdis (2011).
Interleukins, from 1 to 37, and interferon-γ: receptors, functions, and roles in diseases.
  J Allergy Clin Immunol, 127, 701.  
20846897 A.Zdanov (2010).
Structural analysis of cytokines comprising the IL-10 family.
  Cytokine Growth Factor Rev, 21, 325-330.  
20454917 D.B.Trivella, J.R.Ferreira-Júnior, L.Dumoutier, J.C.Renauld, and I.Polikarpov (2010).
Structure and function of interleukin-22 and other members of the interleukin-10 family.
  Cell Mol Life Sci, 67, 2909-2935.  
20889366 G.Gallagher (2010).
Interleukin-19: multiple roles in immune regulation and disease.
  Cytokine Growth Factor Rev, 21, 345-352.  
20712454 H.H.Gad, O.J.Hamming, and R.Hartmann (2010).
The structure of human interferon lambda and what it has taught us.
  J Interferon Cytokine Res, 30, 565-571.  
20655797 K.Witte, E.Witte, R.Sabat, and K.Wolk (2010).
IL-28A, IL-28B, and IL-29: promising cytokines with type I interferon-like properties.
  Cytokine Growth Factor Rev, 21, 237-251.  
20947410 R.P.Donnelly, F.Sheikh, H.Dickensheets, R.Savan, H.A.Young, and M.R.Walter (2010).
Interleukin-26: an IL-10-related cytokine produced by Th17 cells.
  Cytokine Growth Factor Rev, 21, 393-401.  
21112807 R.Sabat (2010).
IL-10 family of cytokines.
  Cytokine Growth Factor Rev, 21, 315-324.  
19734147 K.L.Fuson, M.Zheng, M.Craxton, A.Pataer, R.Ramesh, S.Chada, and R.B.Sutton (2009).
Structural mapping of post-translational modifications in human interleukin-24: role of N-linked glycosylation and disulfide bonds in secretion and activity.
  J Biol Chem, 284, 30526-30533.  
18480827 K.Kingo, R.Mössner, R.Rätsep, K.Raud, U.Krüger, H.Silm, E.Vasar, K.Reich, and S.Kõks (2008).
Association analysis of IL20RA and IL20RB genes in psoriasis.
  Genes Immun, 9, 445-451.  
18177596 D.McCarey, and I.B.McInnes (2007).
Psoriatic arthritis: current topics.
  Curr Rheumatol Rep, 9, 442-448.  
17238830 J.B.Mumm, S.Ekmekcioglu, N.J.Poindexter, S.Chada, and E.A.Grimm (2006).
Soluble human MDA-7/IL-24: characterization of the molecular form(s) inhibiting tumor growth and stimulating monocytes.
  J Interferon Cytokine Res, 26, 877-886.  
17030002 K.Wolk, and R.Sabat (2006).
Interleukin-22: a novel T- and NK-cell derived cytokine that regulates the biology of tissue cells.
  Cytokine Growth Factor Rev, 17, 367-380.  
16784489 L.Hummelshoj, L.P.Ryder, and L.K.Poulsen (2006).
The role of the interleukin-10 subfamily members in immunoglobulin production by human B cells.
  Scand J Immunol, 64, 40-47.  
15922016 C.D.Krause, and S.Pestka (2005).
Evolution of the Class 2 cytokines and receptors, and discovery of new friends and relatives.
  Pharmacol Ther, 106, 299-346.  
16225599 K.Otkjaer, K.Kragballe, A.T.Funding, J.T.Clausen, P.L.Noerby, T.Steiniche, and L.Iversen (2005).
The dynamics of gene expression of interleukin-19 and interleukin-20 and their receptors in psoriasis.
  Br J Dermatol, 153, 911-918.  
15983417 T.Xu, N.J.Logsdon, and M.R.Walter (2005).
Structure of insect-cell-derived IL-22.
  Acta Crystallogr D Biol Crystallogr, 61, 942-950.
PDB code: 1ykb
15032600 S.Pestka, C.D.Krause, D.Sarkar, M.R.Walter, Y.Shi, and P.B.Fisher (2004).
Interleukin-10 and related cytokines and receptors.
  Annu Rev Immunol, 22, 929-979.  
12974481 J.C.Renauld (2003).
Class II cytokine receptors and their ligands: key antiviral and inflammatory modulators.
  Nat Rev Immunol, 3, 667-676.  
14580208 S.Pletnev, E.Magracheva, S.Kozlov, G.Tobin, S.V.Kotenko, A.Wlodawer, and A.Zdanov (2003).
Characterization of the recombinant extracellular domains of human interleukin-20 receptors and their complexes with interleukin-19 and interleukin-20.
  Biochemistry, 42, 12617-12624.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.