PDBsum entry 1mxm

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protein Protein-protein interface(s) links
Membrane protein PDB id
Protein chains
(+ 1 more) 254 a.a.
Superseded by: 2oau 2oau
PDB id:
Name: Membrane protein
Title: Crystal structure of mscs at 3.9 resolution
Structure: Mechanosensitive channel protein. Chain: a, b, c, d, e, f, g. Fragment: residues 27-280. Synonym: mscs. Engineered: yes
Source: Escherichia coli. Bacteria. Gene: yggb. Expressed in: escherichia coli.
Biol. unit: Heptamer (from PQS)
3.90Å     R-factor:   0.329     R-free:   0.356
Authors: R.B.Bass,P.Strop,M.Barclay,D.C.Rees
Key ref:
R.B.Bass et al. (2002). Crystal structure of Escherichia coli MscS, a voltage-modulated and mechanosensitive channel. Science, 298, 1582-1587. PubMed id: 12446901 DOI: 10.1126/science.1077945
02-Oct-02     Release date:   27-Nov-02    
Go to PROCHECK summary

Protein chains
P11666  (YGGB_ECOLI) - 
Key:    Secondary structure


DOI no: 10.1126/science.1077945 Science 298:1582-1587 (2002)
PubMed id: 12446901  
Crystal structure of Escherichia coli MscS, a voltage-modulated and mechanosensitive channel.
R.B.Bass, P.Strop, M.Barclay, D.C.Rees.
The mechanosensitive channel of small conductance (MscS) responds both to stretching of the cell membrane and to membrane depolarization. The crystal structure at 3.9 angstroms resolution demonstrates that Escherichia coli MscS folds as a membrane-spanning heptamer with a large cytoplasmic region. Each subunit contains three transmembrane helices (TM1, -2, and -3), with the TM3 helices lining the pore, while TM1 and TM2, with membrane-embedded arginines, are likely candidates for the tension and voltage sensors. The transmembrane pore, apparently captured in an open state, connects to a large chamber, formed within the cytoplasmic region, that connects to the cytoplasm through openings that may function as molecular filters. Although MscS is likely to be structurally distinct from other ion channels, similarities in gating mechanisms suggest common structural elements.
  Selected figure(s)  
Figure 2.
Fig. 2. Ribbon diagrams of MscS. (A) The polypeptide fold of a MscS subunit, viewed perpendicular to the sevenfold axis, with a rainbow gradient coloring scheme from the NH[2]- terminus (blue) to the COOH-terminus (red). The membrane-spanning helices in the transmembrane domain are labeled TM1, TM2, and TM3. The two remaining domains of the monomer, located in the cytoplasm, as well as location of the strands, are also indicated. Brackets denote approximate dimensions of the molecule in both (A) and (B). (B) The MscS heptamer viewed down the sevenfold axis, looking into the permeation pathway from outside the cell. The coloring of the subunits is as in (C). (C) Stereo side view of the MscS heptamer, viewed from the same direction as in (A), with each subunit represented in a separate color. The orientation of this view is such that the periplasm would be at the top, and the cytoplasm would be at the bottom of the figure. This figure was prepared with MOLSCRIPT (52) and RASTER-3D (53).
Figure 4.
Fig. 4. Model for gating the MscS channel. The transmembrane region of the channel, in an open state, is shown in an expanded view, with one subunit highlighted in dark blue and the remaining six subunits represented in transparent light blue. The side chains of arginine residues at positions 46, 74, and 88 are depicted as larger, colored ball and sticks in the highlighted subunit and as smaller, blue ball and sticks in the remaining subunits. The inferred position of the membrane bilayer is schematically indicated as a gray box. Arrows drawn normal and parallel to the plane of the membrane denote the likely directions of force when depolarization and mechanical stress are applied to the membrane, respectively. Curved arrow schematically illustrates the motion the TM1 and TM2 helices could exhibit in response to either or both of these forces.
  The above figures are reprinted by permission from the AAAs: Science (2002, 298, 1582-1587) copyright 2002.  
  Figures were selected by an automated process.  

Literature references that cite this PDB file's key reference

  PubMed id Reference
20706981 C.E.Price, A.Otto, F.Fusetti, D.Becher, M.Hecker, and A.J.Driessen (2010).
Differential effect of YidC depletion on the membrane proteome of Escherichia coli under aerobic and anaerobic growth conditions.
  Proteomics, 10, 3235-3247.  
20825352 C.Kung, B.Martinac, and S.Sukharev (2010).
Mechanosensitive channels in microbes.
  Annu Rev Microbiol, 64, 313-329.  
20196071 G.Kefala, C.Ahn, M.Krupa, L.Esquivies, I.Maslennikov, W.Kwiatkowski, and S.Choe (2010).
Structures of the OmpF porin crystallized in the presence of foscholine-12.
  Protein Sci, 19, 1117-1125.
PDB codes: 3k19 3k1b
19940001 J.A.Lundbaek, S.A.Collingwood, H.I.Ingólfsson, R.Kapoor, and O.S.Andersen (2010).
Lipid bilayer regulation of membrane protein function: gramicidin channels as molecular force probes.
  J R Soc Interface, 7, 373-395.  
20192782 J.Arnadóttir, and M.Chalfie (2010).
Eukaryotic mechanosensitive channels.
  Annu Rev Biophys, 39, 111-137.  
19826804 K.McLuskey, A.W.Roszak, Y.Zhu, and N.W.Isaacs (2010).
Crystal structures of all-alpha type membrane proteins.
  Eur Biophys J, 39, 723-755.  
20667175 K.R.Vinothkumar, and R.Henderson (2010).
Structures of membrane proteins.
  Q Rev Biophys, 43, 65.  
20356872 K.Yoshimura, and M.Sokabe (2010).
Mechanosensitivity of ion channels based on protein-lipid interactions.
  J R Soc Interface, 7, S307-S320.  
20798307 P.A.Wright (2010).
Chemistry. Opening the door to peptide-based porous solids.
  Science, 329, 1025-1026.  
20607761 R.Krämer (2010).
Bacterial stimulus perception and signal transduction: response to osmotic stress.
  Chem Rec, 10, 217-229.  
20204450 S.J.Facey, and A.Kuhn (2010).
Biogenesis of bacterial inner-membrane proteins.
  Cell Mol Life Sci, 67, 2343-2362.  
20037156 T.Rasmussen, M.D.Edwards, S.S.Black, A.Rasmussen, S.Miller, and I.R.Booth (2010).
Tryptophan in the pore of the mechanosensitive channel MscS: assessment of pore conformations by fluorescence spectroscopy.
  J Biol Chem, 285, 5377-5384.  
20616037 U.Schumann, M.D.Edwards, T.Rasmussen, W.Bartlett, P.van West, and I.R.Booth (2010).
YbdG in Escherichia coli is a threshold-setting mechanosensitive channel with MscM activity.
  Proc Natl Acad Sci U S A, 107, 12664-12669.  
20208543 V.Belyy, A.Anishkin, K.Kamaraju, N.Liu, and S.Sukharev (2010).
The tension-transmitting 'clutch' in the mechanosensitive channel MscS.
  Nat Struct Mol Biol, 17, 451-458.  
20939098 Y.Shafrir, S.Durell, N.Arispe, and H.R.Guy (2010).
Models of membrane-bound Alzheimer's Abeta peptide assemblies.
  Proteins, 78, 3473-3487.  
19383606 A.Anishkin, and S.Sukharev (2009).
State-stabilizing Interactions in Bacterial Mechanosensitive Channel Gating and Adaptation.
  J Biol Chem, 284, 19153-19157.  
19171967 A.T.Brunger, B.Delabarre, J.M.Davies, and W.I.Weis (2009).
X-ray structure determination at low resolution.
  Acta Crystallogr D Biol Crystallogr, 65, 128-133.  
19581598 B.L.Cook, D.Steuerwald, L.Kaiser, J.Graveland-Bikker, M.Vanberghem, A.P.Berke, K.Herlihy, H.Pick, H.Vogel, and S.Zhang (2009).
Large-scale production and study of a synthetic G protein-coupled receptor: human olfactory receptor 17-4.
  Proc Natl Acad Sci U S A, 106, 11925-11930.  
19240214 C.J.De Feo, S.G.Aller, G.S.Siluvai, N.J.Blackburn, and V.M.Unger (2009).
Three-dimensional structure of the human copper transporter hCTR1.
  Proc Natl Acad Sci U S A, 106, 4237-4242.  
19424690 D.Balleza, and F.Gómez-Lagunas (2009).
Conserved motifs in mechanosensitive channels MscL and MscS.
  Eur Biophys J, 38, 1013-1027.  
19686652 H.Machiyama, H.Tatsumi, and M.Sokabe (2009).
Structural changes in the cytoplasmic domain of the mechanosensitive channel MscS during opening.
  Biophys J, 97, 1048-1057.  
19223978 H.Ren, D.Yu, B.Ge, B.Cook, Z.Xu, and S.Zhang (2009).
High-level production, solubilization and purification of synthetic human GPCR chemokine receptors CCR5, CCR3, CXCR4 and CX3CR1.
  PLoS ONE, 4, e4509.  
19923712 J.E.Lee, M.L.Fusco, D.M.Abelson, A.J.Hessell, D.R.Burton, and E.O.Saphire (2009).
Techniques and tactics used in determining the structure of the trimeric ebolavirus glycoprotein.
  Acta Crystallogr D Biol Crystallogr, 65, 1162-1180.
PDB code: 3inu
  19177357 J.Hammon, D.V.Palanivelu, J.Chen, C.Patel, and D.L.Minor (2009).
A green fluorescent protein screen for identification of well-expressed membrane proteins from a cohort of extremophilic organisms.
  Protein Sci, 18, 121-133.  
18368466 J.Siltberg-Liberles, and A.Martinez (2009).
Searching distant homologs of the regulatory ACT domain in phenylalanine hydroxylase.
  Amino Acids, 36, 235-249.  
18849407 R.G.Coleman, and K.A.Sharp (2009).
Finding and characterizing tunnels in macromolecules with application to ion channels and pores.
  Biophys J, 96, 632-645.  
19458714 R.Phillips, T.Ursell, P.Wiggins, and P.Sens (2009).
Emerging roles for lipids in shaping membrane-protein function.
  Nature, 459, 379-385.  
17981908 A.Anishkin, B.Akitake, and S.Sukharev (2008).
Characterization of the resting MscS: modeling and analysis of the closed bacterial mechanosensitive channel of small conductance.
  Biophys J, 94, 1252-1266.  
  18591417 A.Anishkin, K.Kamaraju, and S.Sukharev (2008).
Mechanosensitive channel MscS in the open state: modeling of the transition, explicit simulations, and experimental measurements of conductance.
  J Gen Physiol, 132, 67-83.  
17466568 A.C.Hurst, E.Petrov, A.Kloda, T.Nguyen, L.Hool, and B.Martinac (2008).
MscS, the bacterial mechanosensitive channel of small conductance.
  Int J Biochem Cell Biol, 40, 581-585.  
18535149 A.L.Davidson, E.Dassa, C.Orelle, and J.Chen (2008).
Structure, function, and evolution of bacterial ATP-binding cassette systems.
  Microbiol Mol Biol Rev, 72, 317.  
  18923187 B.Martinac, Y.Saimi, and C.Kung (2008).
Ion channels in microbes.
  Physiol Rev, 88, 1449-1490.  
18786396 C.S.Crowley, M.R.Sawaya, T.A.Bobik, and T.O.Yeates (2008).
Structure of the PduU shell protein from the Pdu microcompartment of Salmonella.
  Structure, 16, 1324-1332.
PDB code: 3cgi
18563247 D.Olschewski, and C.F.Becker (2008).
Chemical synthesis and semisynthesis of membrane proteins.
  Mol Biosyst, 4, 733-740.  
18539773 F.Fornasiero, H.G.Park, J.K.Holt, M.Stadermann, C.P.Grigoropoulos, A.Noy, and O.Bakajin (2008).
Ion exclusion by sub-2-nm carbon nanotube pores.
  Proc Natl Acad Sci U S A, 105, 17250-17255.  
18515388 I.Iscla, R.Wray, and P.Blount (2008).
On the structure of the N-terminal domain of the MscL channel: helical bundle or membrane interface.
  Biophys J, 95, 2283-2291.  
18723509 J.D.Olsen, J.D.Tucker, J.A.Timney, P.Qian, C.Vassilev, and C.N.Hunter (2008).
The organization of LH2 complexes in membranes from Rhodobacter sphaeroides.
  J Biol Chem, 283, 30772-30779.  
18065458 M.D.Edwards, W.Bartlett, and I.R.Booth (2008).
Pore mutations of the Escherichia coli MscS channel affect desensitization but not ionic preference.
  Biophys J, 94, 3003-3013.  
18234836 R.Roth, D.Gillespie, W.Nonner, and R.E.Eisenberg (2008).
Bubbles, gating, and anesthetics in ion channels.
  Biophys J, 94, 4282-4298.  
18584152 T.Burghardt, M.Saller, S.Gürster, D.Müller, C.Meyer, U.Jahn, E.Hochmuth, R.Deutzmann, F.Siedler, P.Babinger, R.Wirth, H.Huber, and R.Rachel (2008).
Insight into the proteome of the hyperthermophilic Crenarchaeon Ignicoccus hospitalis: the major cytosolic and membrane proteins.
  Arch Microbiol, 190, 379-394.  
18310427 T.Hoffmann, C.Boiangiu, S.Moses, and E.Bremer (2008).
Responses of Bacillus subtilis to hypotonic challenges: physiological contributions of mechanosensitive channels to cellular survival.
  Appl Environ Microbiol, 74, 2454-2460.  
17993482 T.Nomura, M.Sokabe, and K.Yoshimura (2008).
Interaction between the cytoplasmic and transmembrane domains of the mechanosensitive channel MscS.
  Biophys J, 94, 1638-1645.  
18343404 V.Vásquez, M.Sotomayor, D.M.Cortes, B.Roux, K.Schulten, and E.Perozo (2008).
Three-dimensional architecture of membrane-embedded MscS in the closed conformation.
  J Mol Biol, 378, 55-70.  
18755978 V.Vásquez, M.Sotomayor, J.Cordero-Morales, K.Schulten, and E.Perozo (2008).
A structural mechanism for MscS gating in lipid bilayers.
  Science, 321, 1210-1214.  
18723622 Y.Feng, M.Li, H.Zhang, B.Zheng, H.Han, C.Wang, J.Yan, J.Tang, and G.F.Gao (2008).
Functional definition and global regulation of Zur, a zinc uptake regulator in a Streptococcus suis serotype 2 strain causing streptococcal toxic shock syndrome.
  J Bacteriol, 190, 7567-7578.  
18787764 Y.Tang, J.Yoo, A.Yethiraj, Q.Cui, and X.Chen (2008).
Mechanosensitive channels: insights from continuum-based simulations.
  Cell Biochem Biophys, 52, 1.  
  17261841 A.L.Brown, S.M.Fernandez-Illescas, Z.Liao, and M.B.Goodman (2007).
Gain-of-function mutations in the MEC-4 DEG/ENaC sensory mechanotransduction channel alter gating and drug blockade.
  J Gen Physiol, 129, 161-173.  
18000058 A.Nakamura, C.Wada, and K.Miki (2007).
Structural basis for regulation of bifunctional roles in replication initiator protein.
  Proc Natl Acad Sci U S A, 104, 18484-18489.
PDB code: 2z9o
18007608 A.T.Brunger (2007).
Version 1.2 of the Crystallography and NMR system.
  Nat Protoc, 2, 2728-2733.  
18037888 B.Akitake, A.Anishkin, N.Liu, and S.Sukharev (2007).
Straightening and sequential buckling of the pore-lining helices define the gating cycle of MscS.
  Nat Struct Mol Biol, 14, 1141-1149.  
17277184 B.Akitake, R.E.Spelbrink, A.Anishkin, J.A.Killian, Kruijff, and S.Sukharev (2007).
2,2,2-Trifluoroethanol changes the transition kinetics and subunit interactions in the small bacterial mechanosensitive channel MscS.
  Biophys J, 92, 2771-2784.  
17493135 C.Li, M.D.Edwards, H.Jeong, H.Jeong, J.Roth, and I.R.Booth (2007).
Identification of mutations that alter the gating of the Escherichia coli mechanosensitive channel protein, MscK.
  Mol Microbiol, 64, 560-574.  
17562317 C.P.Arthur, and M.H.Stowell (2007).
Structure of synaptophysin: a hexameric MARVEL-domain channel protein.
  Structure, 15, 707-714.  
17490609 G.L.Lorca, R.D.Barabote, V.Zlotopolski, C.Tran, B.Winnen, R.N.Hvorup, A.J.Stonestrom, E.Nguyen, L.W.Huang, D.S.Kim, and M.H.Saier (2007).
Transport capabilities of eleven gram-positive bacteria: comparative genomic analyses.
  Biochim Biophys Acta, 1768, 1342-1366.  
17505523 I.R.Booth, M.D.Edwards, S.Black, U.Schumann, and S.Miller (2007).
Mechanosensitive channels in bacteria: signs of closure?
  Nat Rev Microbiol, 5, 431-440.  
18075585 J.P.Morth, B.P.Pedersen, M.S.Toustrup-Jensen, T.L.Sørensen, J.Petersen, J.P.Andersen, B.Vilsen, and P.Nissen (2007).
Crystal structure of the sodium-potassium pump.
  Nature, 450, 1043-1049.
PDB codes: 3b8e 3kdp
17078969 M.A.White, K.M.Clark, E.J.Grayhack, and M.E.Dumont (2007).
Characteristics affecting expression and solubilization of yeast membrane proteins.
  J Mol Biol, 365, 621-636.  
17114233 M.Sotomayor, V.Vásquez, E.Perozo, and K.Schulten (2007).
Ion conduction through MscS as determined by electrophysiology and simulation.
  Biophys J, 92, 886-902.  
17242512 P.Strop, M.R.Brzustowicz, and A.T.Brunger (2007).
Ab initio molecular-replacement phasing for symmetric helical membrane proteins.
  Acta Crystallogr D Biol Crystallogr, 63, 188-196.  
17011510 S.L.Rusch, and D.A.Kendall (2007).
Oligomeric states of the SecA and SecYEG core components of the bacterial Sec translocon.
  Biochim Biophys Acta, 1768, 5.  
17288559 T.Pisareva, M.Shumskaya, G.Maddalo, L.Ilag, and B.Norling (2007).
Proteomics of Synechocystis sp. PCC 6803. Identification of novel integral plasma membrane proteins.
  FEBS J, 274, 791-804.  
17534481 X.Liang, D.J.Campopiano, and P.J.Sadler (2007).
Metals in membranes.
  Chem Soc Rev, 36, 968-992.  
17389370 Y.Nakayama, K.Fujiu, M.Sokabe, and K.Yoshimura (2007).
Molecular and electrophysiological characterization of a mechanosensitive channel expressed in the chloroplasts of Chlamydomonas.
  Proc Natl Acad Sci U S A, 104, 5883-5888.  
16599805 A.H.Zewail (2006).
4D ultrafast electron diffraction, crystallography, and microscopy.
  Annu Rev Phys Chem, 57, 65.  
16731967 A.L.Lomize, I.D.Pogozheva, M.A.Lomize, and H.I.Mosberg (2006).
Positioning of proteins in membranes: a computational approach.
  Protein Sci, 15, 1318-1333.  
16538695 A.Mourot, T.Grutter, M.Goeldner, and F.Kotzyba-Hibert (2006).
Dynamic structural investigations on the torpedo nicotinic acetylcholine receptor by time-resolved photoaffinity labeling.
  Chembiochem, 7, 570-583.  
16815920 A.Oberai, Y.Ihm, S.Kim, and J.U.Bowie (2006).
A limited universe of membrane protein families and folds.
  Protein Sci, 15, 1723-1734.  
16552147 A.P.Yeh, A.McMillan, and M.H.Stowell (2006).
Rapid and simple protein-stability screens: application to membrane proteins.
  Acta Crystallogr D Biol Crystallogr, 62, 451-457.  
16855310 B.DeLaBarre, and A.T.Brunger (2006).
Considerations for the refinement of low-resolution crystal structures.
  Acta Crystallogr D Biol Crystallogr, 62, 923-932.  
16836758 D.Casciari, M.Seeber, and F.Fanelli (2006).
Quaternary structure predictions of transmembrane proteins starting from the monomer: a docking-based approach.
  BMC Bioinformatics, 7, 340.  
17215877 E.O.Oloo, C.Kandt, M.L.O'Mara, and D.P.Tieleman (2006).
Computer simulations of ABC transporter components.
  Biochem Cell Biol, 84, 900-911.  
16493417 E.Perozo (2006).
Gating prokaryotic mechanosensitive channels.
  Nat Rev Mol Cell Biol, 7, 109-119.  
16401419 E.S.Haswell, and E.M.Meyerowitz (2006).
MscS-like proteins control plastid size and shape in Arabidopsis thaliana.
  Curr Biol, 16, 1.  
17159147 L.Li, D.Mustafi, Q.Fu, V.Tereshko, D.L.Chen, J.D.Tice, and R.F.Ismagilov (2006).
Nanoliter microfluidic hybrid method for simultaneous screening and optimization validated with crystallization of membrane proteins.
  Proc Natl Acad Sci U S A, 103, 19243-19248.
PDB code: 2i5n
16513774 M.Sotomayor, T.A.van der Straaten, U.Ravaioli, and K.Schulten (2006).
Electrostatic properties of the mechanosensitive channel of small conductance MscS.
  Biophys J, 90, 3496-3510.  
16829701 O.Beckstein, and M.S.Sansom (2006).
A hydrophobic gate in an ion channel: the closed state of the nicotinic acetylcholine receptor.
  Phys Biol, 3, 147-159.  
17021800 O.P.Hamill (2006).
Twenty odd years of stretch-sensitive channels.
  Pflugers Arch, 453, 333-351.  
16500980 S.A.Spronk, D.E.Elmore, and D.A.Dougherty (2006).
Voltage-dependent hydration and conduction properties of the hydrophobic pore of the mechanosensitive channel of small conductance.
  Biophys J, 90, 3555-3569.  
16501047 S.G.Aller, and V.M.Unger (2006).
Projection structure of the human copper transporter CTR1 at 6-A resolution reveals a compact trimer with a novel channel-like architecture.
  Proc Natl Acad Sci U S A, 103, 3627-3632.  
16861270 T.Nomura, M.Sokabe, and K.Yoshimura (2006).
Lipid-protein interaction of the MscS mechanosensitive channel examined by scanning mutagenesis.
  Biophys J, 91, 2874-2881.  
16006117 A.Anishkin, and C.Kung (2005).
Microbial mechanosensation.
  Curr Opin Neurobiol, 15, 397-405.  
  15684093 A.Anishkin, C.S.Chiang, S.Sukharev, and S.I.Sukharev (2005).
Gain-of-function mutations reveal expanded intermediate states and a sequential action of two gates in MscL.
  J Gen Physiol, 125, 155-170.  
15834558 A.G.Macdonald, and B.Martinac (2005).
Effect of high hydrostatic pressure on the bacterial mechanosensitive channel MscS.
  Eur Biophys J, 34, 434-441.  
15711166 A.Pohorille, K.Schweighofer, and M.A.Wilson (2005).
The origin and early evolution of membrane channels.
  Astrobiology, 5, 1.  
15698560 A.T.Brunger (2005).
Low-resolution crystallography is coming of age.
  Structure, 13, 171-172.  
  15657299 B.Akitake, A.Anishkin, S.Sukharev, and S.Sukharev (2005).
The "dashpot" mechanism of stretch-dependent gating in MscS.
  J Gen Physiol, 125, 143-154.  
15702067 B.Martinac (2005).
Structural plasticity in MS channels.
  Nat Struct Mol Biol, 12, 104-105.  
16154351 B.Zheng, C.J.Gerdts, and R.F.Ismagilov (2005).
Using nanoliter plugs in microfluidics to facilitate and understand protein crystallization.
  Curr Opin Struct Biol, 15, 548-555.  
16079835 C.Kung (2005).
A possible unifying principle for mechanosensation.
  Nature, 436, 647-654.  
16006629 C.Peter, and G.Hummer (2005).
Ion transport through membrane-spanning nanopores studied by molecular dynamics simulations and continuum electrostatics calculations.
  Biophys J, 89, 2222-2234.  
15974784 G.V.Miloshevsky, and P.C.Jordan (2005).
Permeation and gating in proteins: kinetic Monte Carlo reaction path following.
  J Chem Phys, 122, 214901.  
16043693 K.Oxenoid, and J.J.Chou (2005).
The structure of phospholamban pentamer reveals a channel-like architecture in membranes.
  Proc Natl Acad Sci U S A, 102, 10870-10875.
PDB code: 1zll
15556982 L.Saiz, and M.L.Klein (2005).
The transmembrane domain of the acetylcholine receptor: insights from simulations on synthetic peptide models.
  Biophys J, 88, 959-970.  
15665866 M.D.Edwards, Y.Li, S.Kim, S.Miller, W.Bartlett, S.Black, S.Dennison, I.Iscla, P.Blount, J.U.Bowie, and I.R.Booth (2005).
Pivotal role of the glycine-rich TM3 helix in gating the MscS mechanosensitive channel.
  Nat Struct Mol Biol, 12, 113-119.  
16026885 M.M.Kuo, W.J.Haynes, S.H.Loukin, C.Kung, and Y.Saimi (2005).
Prokaryotic K(+) channels: from crystal structures to diversity.
  FEMS Microbiol Rev, 29, 961-985.  
15858647 M.Nishihara, F.Perret, T.Takeuchi, S.Futaki, A.N.Lazar, A.W.Coleman, N.Sakai, and S.Matile (2005).
Arginine magic with new counterions up the sleeve.
  Org Biomol Chem, 3, 1659-1669.  
16247816 N.Madhavan, E.C.Robert, and M.S.Gin (2005).
A highly active anion-selective aminocyclodextrin ion channel.
  Angew Chem Int Ed Engl, 44, 7584-7587.  
15816168 O.S.Andersen, R.E.Koeppe, and B.Roux (2005).
Gramicidin channels.
  IEEE Trans Nanobioscience, 4, 10-20.  
15542561 P.Wiggins, and R.Phillips (2005).
Membrane-protein interactions in mechanosensitive channels.
  Biophys J, 88, 880-902.  
16179394 S.Kim, T.J.Jeon, A.Oberai, D.Yang, J.J.Schmidt, and J.U.Bowie (2005).
Transmembrane glycine zippers: physiological and pathological roles in membrane proteins.
  Proc Natl Acad Sci U S A, 102, 14278-14283.  
15770478 T.Nguyen, B.Clare, W.Guo, and B.Martinac (2005).
The effects of parabens on the mechanosensitive channels of E. coli.
  Eur Biophys J, 34, 389-395.  
15665126 W.Grajkowski, A.Kubalski, and P.Koprowski (2005).
Surface changes of the mechanosensitive channel MscS upon its activation, inactivation, and closing.
  Biophys J, 88, 3050-3059.  
15111405 A.Anishkin, S.Sukharev, and S.Sukharev (2004).
Water dynamics and dewetting transitions in the small mechanosensitive channel MscS.
  Biophys J, 86, 2883-2895.  
14766176 A.I.Sobolevsky, M.V.Yelshansky, and L.P.Wollmuth (2004).
The outer pore of the glutamate receptor channel has 2-fold rotational symmetry.
  Neuron, 41, 367-378.  
14766985 A.M.Slovic, H.Kono, J.D.Lear, J.G.Saven, and W.F.DeGrado (2004).
Computational design of water-soluble analogues of the potassium channel KcsA.
  Proc Natl Acad Sci U S A, 101, 1828-1833.  
15228520 C.Kung, and P.Blount (2004).
Channels in microbes: so many holes to fill.
  Mol Microbiol, 53, 373-380.  
15041744 D.Clayton, G.Shapovalov, J.A.Maurer, D.A.Dougherty, H.A.Lester, and G.G.Kochendoerfer (2004).
Total chemical synthesis and electrophysiological characterization of mechanosensitive channels from Escherichia coli and Mycobacterium tuberculosis.
  Proc Natl Acad Sci U S A, 101, 4764-4769.  
15525509 F.Guo, A.R.Gooding, and T.R.Cech (2004).
Structure of the Tetrahymena ribozyme: base triple sandwich and metal ion at the active site.
  Mol Cell, 16, 351-362.
PDB code: 1x8w
  15277576 G.Shapovalov, and H.A.Lester (2004).
Gating transitions in bacterial ion channels measured at 3 microns resolution.
  J Gen Physiol, 124, 151-161.  
15458400 K.J.Hellingwerf (2004).
A network of net-workers: report of the Euresco conference on 'Bacterial Neural Networks' held at San Feliu (Spain) from 8 to 14 May 2004.
  Mol Microbiol, 54, 2.  
15550946 K.J.Swartz (2004).
Towards a structural view of gating in potassium channels.
  Nat Rev Neurosci, 5, 905-916.  
15063854 M.D.Edwards, I.R.Booth, and S.Miller (2004).
Gating the bacterial mechanosensitive channels: MscS a new paradigm?
  Curr Opin Microbiol, 7, 163-167.  
15591352 M.Grabe, H.Lecar, Y.N.Jan, and L.Y.Jan (2004).
A quantitative assessment of models for voltage-dependent gating of ion channels.
  Proc Natl Acad Sci U S A, 101, 17640-17645.  
15568015 M.J.Conroy, S.J.Jamieson, D.Blakey, T.Kaufmann, A.Engel, D.Fotiadis, M.Merrick, and P.A.Bullough (2004).
Electron and atomic force microscopy of the trimeric ammonium transporter AmtB.
  EMBO Rep, 5, 1153-1158.  
15502395 M.Sokabe, K.Naruse, and T.Qiong-Yao (2004).
[A new mechanosensitive channel SAKCA and a new MS channel blocker GsTMx-4]
  Nippon Yakurigaku Zasshi, 124, 301-310.  
15339798 M.Sotomayor, and K.Schulten (2004).
Molecular dynamics study of gating in the mechanosensitive channel of small conductance MscS.
  Biophys J, 87, 3050-3065.  
15071758 R.J.Law, and M.S.Sansom (2004).
Homology modelling and molecular dynamics simulations: comparative studies of human aquaporin-1.
  Eur Biophys J, 33, 477-489.  
15385536 S.G.Aller, E.T.Eng, C.J.De Feo, and V.M.Unger (2004).
Eukaryotic CTR copper uptake transporters require two faces of the third transmembrane domain for helix packing, oligomerization, and function.
  J Biol Chem, 279, 53435-53441.  
15067113 S.Kim, A.K.Chamberlain, and J.U.Bowie (2004).
Membrane channel structure of Helicobacter pylori vacuolating toxin: role of multiple GXXXG motifs in cylindrical channels.
  Proc Natl Acad Sci U S A, 101, 5988-5991.
PDB code: 1sew
15257761 V.Anantharaman, and L.Aravind (2004).
Novel conserved domains in proteins with predicted roles in eukaryotic cell-cycle regulation, decapping and RNA stability.
  BMC Genomics, 5, 45.  
16204828 V.S.Markin, and F.Sachs (2004).
Thermodynamics of mechanosensitivity.
  Phys Biol, 1, 110-124.  
12547440 A.Giorgetti, and P.Carloni (2003).
Molecular modeling of ion channels: structural predictions.
  Curr Opin Chem Biol, 7, 150-156.  
14573948 A.González (2003).
Optimizing data collection for structure determination.
  Acta Crystallogr D Biol Crystallogr, 59, 1935-1942.  
12948770 A.R.Curran, and D.M.Engelman (2003).
Sequence motifs, polar interactions and conformational changes in helical membrane proteins.
  Curr Opin Struct Biol, 13, 412-417.  
12949490 B.DeLaBarre, and A.T.Brunger (2003).
Complete structure of p97/valosin-containing protein reveals communication between nucleotide domains.
  Nat Struct Biol, 10, 856-863.
PDB code: 1oz4
12768203 C.Miller (2003).
A charged view of voltage-gated ion channels.
  Nat Struct Biol, 10, 422-424.  
12668760 C.Mura, M.Phillips, A.Kozhukhovsky, and D.Eisenberg (2003).
Structure and assembly of an augmented Sm-like archaeal protein 14-mer.
  Proc Natl Acad Sci U S A, 100, 4539-4544.
PDB code: 1m5q
12885631 C.W.Wolgemuth, O.Igoshin, and G.Oster (2003).
The motility of mollicutes.
  Biophys J, 85, 828-842.  
12948773 E.Perozo, and D.C.Rees (2003).
Structure and mechanism in prokaryotic mechanosensitive channels.
  Curr Opin Struct Biol, 13, 432-442.  
14507677 J.Gullingsrud, and K.Schulten (2003).
Gating of MscL studied by steered molecular dynamics.
  Biophys J, 85, 2087-2099.  
14657351 L.Cristian, J.D.Lear, and W.F.DeGrado (2003).
Use of thiol-disulfide equilibria to measure the energetics of assembly of transmembrane helices in phospholipid bilayers.
  Proc Natl Acad Sci U S A, 100, 14772-14777.  
12815340 M.Machius (2003).
Structural biology: a high-tech tool for biomedical research.
  Curr Opin Nephrol Hypertens, 12, 431-438.  
14671322 N.R.Stokes, H.D.Murray, C.Subramaniam, R.L.Gourse, P.Louis, W.Bartlett, S.Miller, and I.R.Booth (2003).
A role for mechanosensitive channels in survival of stationary phase: regulation of channel expression by RpoS.
  Proc Natl Acad Sci U S A, 100, 15959-15964.  
12740433 O.Beckstein, and M.S.Sansom (2003).
Liquid-vapor oscillations of water in hydrophobic nanopores.
  Proc Natl Acad Sci U S A, 100, 7063-7068.  
12867984 S.P.Kelley, J.I.Dunlop, E.F.Kirkness, J.J.Lambert, and J.A.Peters (2003).
A cytoplasmic region determines single-channel conductance in 5-HT3 receptors.
  Nature, 424, 321-324.  
14581228 T.Zhang, and J.S.Johansson (2003).
An isothermal titration calorimetry study on the binding of four volatile general anesthetics to the hydrophobic core of a four-alpha-helix bundle protein.
  Biophys J, 85, 3279-3285.  
12771382 X.L.Zhou, A.F.Batiza, S.H.Loukin, C.P.Palmer, C.Kung, and Y.Saimi (2003).
The transient receptor potential channel on the yeast vacuole is mechanosensitive.
  Proc Natl Acad Sci U S A, 100, 7105-7110.  
12721619 Y.Jiang, V.Ruta, J.Chen, A.Lee, and R.MacKinnon (2003).
The principle of gating charge movement in a voltage-dependent K+ channel.
  Nature, 423, 42-48.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.