PDBsum entry: 1mp1

RNA binding protein

PDB id: 1mp1

Contents

Protein chain

111 a.a. *

* Residue conservation analysis

PDB id:

1mp1

Name:

RNA binding protein

Title:

Solution structure of the pwi motif from srm160

Structure:

Ser/arg-related nuclear matrix protein. Chain: a. Fragment: pwi motif (residues 27-134). Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: srm160. Expressed in: escherichia coli. Expression_system_taxid: 562.

NMR struc:

20 models

Authors:

B.R.Szymczyna,J.Bowman,S.Mccracken,A.Pineda-Lucena,Y.Lu, B.Cox,M.Lambermon,B.R.Graveley,C.H.Arrowsmith,B.J.Blencowe

Key ref:

B.R.Szymczyna et al. (2003). Structure and function of the PWI motif: a novel nucleic acid-binding domain that facilitates pre-mRNA processing. Genes Dev, 17, 461-475. PubMed id: 12600940 DOI: 10.1101/gad.1060403

Date:

11-Sep-02 Release date: 16-Sep-03

Protein chain

Q8IYB3  (SRRM1_HUMAN) -  Serine/arginine repetitive matrix protein 1

Seq: 904 a.a.

Struc: 111 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

 Gene Ontology (GO) functional annotation 

• Biological process: mRNA processing (1 term)

DOI no: 10.1101/gad.1060403

Genes Dev 17:461-475 (2003)

PubMed id: 12600940

Structure and function of the PWI motif: a novel nucleic acid-binding domain that facilitates pre-mRNA processing.

B.R.Szymczyna, J.Bowman, S.McCracken, A.Pineda-Lucena, Y.Lu, B.Cox, M.Lambermon, B.R.Graveley, C.H.Arrowsmith, B.J.Blencowe.

ABSTRACT

The PWI motif is a highly conserved domain of unknown function in the SRm160 splicing and 3'-end cleavage-stimulatory factor, as well as in several other known or putative pre-mRNA processing components. We show here that the PWI motif is a new type of RNA/DNA-binding domain that has an equal preference for single- and double-stranded nucleic acids. Deletion of the motif prevents SRm160 from binding RNA and stimulating 3'-end cleavage, and its substitution with a heterologous RNA-binding domain restores these functions. The NMR solution structure of the SRm160-PWI motif reveals a novel, four-helix bundle and represents the first example of an alpha-helical fold that can bind single-stranded (ss)RNA. Structure-guided mutagenesis indicates that the same surface is involved in RNA and DNA binding and requires the cooperative action of a highly conserved, adjacent basic region. Thus, the PWI motif is a novel type of nucleic acid-binding domain that likely has multiple important functions in pre-mRNA processing, including SRm160-dependent stimulation of 3'-end formation.