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PDBsum entry 1lox

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protein ligands metals links
Oxidoreductase PDB id
1lox
Jmol
Contents
Protein chain
647 a.a. *
Ligands
RS7
Metals
FE2
Waters ×94
* Residue conservation analysis
PDB id:
1lox
Name: Oxidoreductase
Title: Rabbit reticulocyte 15-lipoxygenase
Structure: 15-lipoxygenase. Chain: a. Synonym: 15lox. Other_details: 15lox is bound to a competitive inhibitor which has the residue number 841
Source: Oryctolagus cuniculus. Rabbit. Organism_taxid: 9986. Cell: reticulocyte. Cellular_location: cytoplasm
Resolution:
2.40Å     R-factor:   0.198     R-free:   0.247
Authors: S.A.Gillmor,A.Villasenor,R.J.Fletterick,E.Sigal,M.F.Browner
Key ref: S.A.Gillmor et al. (1997). The structure of mammalian 15-lipoxygenase reveals similarity to the lipases and the determinants of substrate specificity. Nat Struct Biol, 4, 1003-1009. PubMed id: 9406550
Date:
06-Oct-97     Release date:   04-Nov-98    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P12530  (LOX15_RABIT) -  Arachidonate 15-lipoxygenase
Seq:
Struc:
 
Seq:
Struc:
663 a.a.
647 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Enzyme reactions 
   Enzyme class 2: E.C.1.13.11.31  - Arachidonate 12-lipoxygenase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Arachidonate + O2 = (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa- 5,8,10,14-tetraenoate
Arachidonate
Bound ligand (Het Group name = RS7)
matches with 86.00% similarity
+ O(2)
= (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa- 5,8,10,14-tetraenoate
      Cofactor: Fe cation
   Enzyme class 3: E.C.1.13.11.33  - Arachidonate 15-lipoxygenase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Arachidonate + O2 = (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa- 5,8,11,13-tetraenoate
Arachidonate
+ O(2)
= (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa- 5,8,11,13-tetraenoate
      Cofactor: Fe cation
Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     membrane   6 terms 
  Biological process     regulation of peroxisome proliferator activated receptor signaling pathway   22 terms 
  Biochemical function     lipid binding     9 terms  

 

 
    reference    
 
 
Nat Struct Biol 4:1003-1009 (1997)
PubMed id: 9406550  
 
 
The structure of mammalian 15-lipoxygenase reveals similarity to the lipases and the determinants of substrate specificity.
S.A.Gillmor, A.Villaseñor, R.Fletterick, E.Sigal, M.F.Browner.
 
  ABSTRACT  
 
Here we report the first structure of a mammalian 15-lipoxygenase. The protein is composed of two domains; a catalytic domain and a previously unrecognized beta-barrel domain. The N-terminal beta-barrel domain has topological and sequence identify to a domain in the mammalian lipases, suggesting that these domains may have similar functions in vivo. Within the C-terminal domain, the lipoxygenase substrate binding site is a hydrophobic pocket defined by a bound inhibitor. Arachidonic acid can be docked into this deep hydrophobic pocket with the methyl end extending down into the bottom of the pocket and the acid end tethered by a conserved basic residue on the surface of the enzyme. This structure provides a unifying hypothesis for the positional specificity of mammalian lipoxygenases.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
21257189 L.E.Chohany, K.A.Bishop, H.Camic, S.J.Sup, P.M.Findeis, and C.H.Clapp (2011).
Cationic substrates of soybean lipoxygenase-1.
  Bioorg Chem, 39, 94.  
21233389 N.C.Gilbert, S.G.Bartlett, M.T.Waight, D.B.Neau, W.E.Boeglin, A.R.Brash, and M.E.Newcomer (2011).
The structure of human 5-lipoxygenase.
  Science, 331, 217-219.
PDB code: 3o8y
21360129 S.T.Moin, T.S.Hofer, R.Sattar, and Z.Ul-Haq (2011).
Molecular dynamics simulation of mammalian 15S-lipoxygenase with AMBER force field.
  Eur Biophys J, 40, 715-726.  
20673209 E.V.Osipova, I.R.Chechetkin, Y.V.Gogolev, and N.B.Tarasova (2010).
Recombinant maize 9-lipoxygenase: expression, purification, and properties.
  Biochemistry (Mosc), 75, 861-865.  
20422083 F.Mei, C.Ou, G.Wu, L.Cao, F.Han, X.Meng, J.Li, D.Li, and Z.Liao (2010).
Non-heme iron(II/III) complexes that model the reactivity of lipoxygenase with a redox switch.
  Dalton Trans, 39, 4267-4269.  
19807693 J.Esser, M.Rakonjac, B.Hofmann, L.Fischer, P.Provost, G.Schneider, D.Steinhilber, B.Samuelsson, and O.Rådmark (2010).
Coactosin-like protein functions as a stabilizing chaperone for 5-lipoxygenase: role of tryptophan 102.
  Biochem J, 425, 265-274.  
19683507 J.Rapp, S.Xu, A.M.Sharp, W.P.Griffith, Y.W.Kim, and M.O.Funk (2009).
EPR spectroscopy and electrospray ionization mass spectrometry reveal distinctive features of the iron site in leukocyte 12-lipoxygenase.
  Arch Biochem Biophys, 490, 50-56.  
19131948 K.M.Eckl, S.de Juanes, J.Kurtenbach, M.Nätebus, J.Lugassy, V.Oji, H.Traupe, M.L.Preil, F.Martínez, J.Smolle, A.Harel, P.Krieg, E.Sprecher, and H.C.Hennies (2009).
Molecular analysis of 250 patients with autosomal recessive congenital ichthyosis: evidence for mutation hotspots in ALOXE3 and allelic heterogeneity in ALOX12B.
  J Invest Dermatol, 129, 1421-1428.  
19244215 M.W.Buczynski, D.S.Dumlao, and E.A.Dennis (2009).
Thematic Review Series: Proteomics. An integrated omics analysis of eicosanoid biology.
  J Lipid Res, 50, 1015-1038.  
19732867 N.Grillet, M.Schwander, M.S.Hildebrand, A.Sczaniecka, A.Kolatkar, J.Velasco, J.A.Webster, K.Kahrizi, H.Najmabadi, W.J.Kimberling, D.Stephan, M.Bahlo, T.Wiltshire, L.M.Tarantino, P.Kuhn, R.J.Smith, and U.Müller (2009).
Mutations in LOXHD1, an evolutionarily conserved stereociliary protein, disrupt hair cell function in mice and cause progressive hearing loss in humans.
  Am J Hum Genet, 85, 328-337.  
  18987389 O.Rådmark, and B.Samuelsson (2009).
5-Lipoxygenase: mechanisms of regulation.
  J Lipid Res, 50, S40-S45.  
19141279 R.Recacha, A.Boulet, F.Jollivet, S.Monier, A.Houdusse, B.Goud, and A.R.Khan (2009).
Structural basis for recruitment of Rab6-interacting protein 1 to Golgi via a RUN domain.
  Structure, 17, 21-30.
PDB code: 3cwz
19525377 Y.Chawengsub, K.M.Gauthier, and W.B.Campbell (2009).
Role of arachidonic acid lipoxygenase metabolites in the regulation of vascular tone.
  Am J Physiol Heart Circ Physiol, 297, H495-H507.  
18547056 C.Jacquot, A.T.Wecksler, C.M.McGinley, E.N.Segraves, T.R.Holman, and W.A.van der Donk (2008).
Isotope sensitive branching and kinetic isotope effects in the reaction of deuterated arachidonic acids with human 12- and 15-lipoxygenases.
  Biochemistry, 47, 7295-7303.  
18972057 C.Jacquot, C.M.McGinley, E.Plata, T.R.Holman, and W.A.van der Donk (2008).
Synthesis of 11-thialinoleic acid and 14-thialinoleic acid, inhibitors of soybean and human lipoxygenases.
  Org Biomol Chem, 6, 4242-4252.  
18785202 H.Zhu, W.Glasgow, M.D.George, K.Chrysovergis, K.Olden, J.D.Roberts, and T.Eling (2008).
15-Lipoxygenase-1 activates tumor suppressor p53 independent of enzymatic activity.
  Int J Cancer, 123, 2741-2749.  
17847087 J.Choi, J.K.Chon, S.Kim, and W.Shin (2008).
Conformational flexibility in mammalian 15S-lipoxygenase: Reinterpretation of the crystallographic data.
  Proteins, 70, 1023-1032.
PDB code: 2p0m
  18625039 L.H.Heckmann, R.M.Sibly, M.J.Timmermans, and A.Callaghan (2008).
Outlining eicosanoid biosynthesis in the crustacean Daphnia.
  Front Zool, 5, 11.  
18311922 M.V.Turman, P.J.Kingsley, C.A.Rouzer, B.F.Cravatt, and L.J.Marnett (2008).
Oxidative metabolism of a fatty acid amide hydrolase-regulated lipid, arachidonoyltaurine.
  Biochemistry, 47, 3917-3925.  
18785758 N.C.Gilbert, M.Niebuhr, H.Tsuruta, T.Bordelon, O.Ridderbusch, A.Dassey, A.R.Brash, S.G.Bartlett, and M.E.Newcomer (2008).
A covalent linker allows for membrane targeting of an oxylipin biosynthetic complex.
  Biochemistry, 47, 10665-10676.
PDB code: 3dy5
18658287 S.Horowitz Brown, R.Zarnowski, W.C.Sharpee, and N.P.Keller (2008).
Morphological transitions governed by density dependence and lipoxygenase activity in Aspergillus flavus.
  Appl Environ Microbiol, 74, 5674-5685.  
17959182 T.L.Assimes, J.W.Knowles, J.R.Priest, A.Basu, A.Borchert, K.A.Volcik, M.L.Grove, H.K.Tabor, A.Southwick, R.Tabibiazar, S.Sidney, E.Boerwinkle, A.S.Go, C.Iribarren, M.A.Hlatky, S.P.Fortmann, R.M.Myers, H.Kuhn, N.Risch, and T.Quertermous (2008).
A near null variant of 12/15-LOX encoded by a novel SNP in ALOX15 and the risk of coronary artery disease.
  Atherosclerosis, 198, 136-144.  
18640486 Y.Liu, B.I.Freedman, K.P.Burdon, C.D.Langefeld, T.Howard, D.Herrington, D.C.Goff, D.W.Bowden, L.E.Wagenknecht, C.C.Hedrick, and S.S.Rich (2008).
Association of arachidonate 12-lipoxygenase genotype variation and glycemic control with albuminuria in type 2 diabetes.
  Am J Kidney Dis, 52, 242-250.  
18070874 Y.Zheng, W.E.Boeglin, C.Schneider, and A.R.Brash (2008).
A 49-kDa mini-lipoxygenase from Anabaena sp. PCC 7120 retains catalytically complete functionality.
  J Biol Chem, 283, 5138-5147.  
18078545 C.D.Putnam, M.Hammel, G.L.Hura, and J.A.Tainer (2007).
X-ray solution scattering (SAXS) combined with crystallography and computation: defining accurate macromolecular structures, conformations and assemblies in solution.
  Q Rev Biophys, 40, 191-285.  
17524979 C.Schneider, D.A.Pratt, N.A.Porter, and A.R.Brash (2007).
Control of oxygenation in lipoxygenase and cyclooxygenase catalysis.
  Chem Biol, 14, 473-488.  
  18007054 D.B.Neau, N.C.Gilbert, S.G.Bartlett, A.Dassey, and M.E.Newcomer (2007).
Improving protein crystal quality by selective removal of a Ca(2+)-dependent membrane-insertion loop.
  Acta Crystallogr Sect F Struct Biol Cryst Commun, 63, 972-975.  
17493578 J.J.Prusakiewicz, M.V.Turman, A.Vila, H.L.Ball, A.H.Al-Mestarihi, V.Di Marzo, and L.J.Marnett (2007).
Oxidative metabolism of lipoamino acids and vanilloids by lipoxygenases and cyclooxygenases.
  Arch Biochem Biophys, 464, 260-268.  
17675410 J.Saam, I.Ivanov, M.Walther, H.G.Holzhütter, and H.Kuhn (2007).
Molecular dioxygen enters the active site of 12/15-lipoxygenase via dynamic oxygen access channels.
  Proc Natl Acad Sci U S A, 104, 13319-13324.  
17576065 O.Rådmark, O.Werz, D.Steinhilber, and B.Samuelsson (2007).
5-Lipoxygenase: regulation of expression and enzyme activity.
  Trends Biochem Sci, 32, 332-341.  
17608719 S.Nigam, M.P.Zafiriou, R.Deva, R.Ciccoli, and R.Roux-Van der Merwe (2007).
Structure, biochemistry and biology of hepoxilins: an update.
  FEBS J, 274, 3503-3512.  
17022084 B.Youn, G.E.Sellhorn, R.J.Mirchel, B.J.Gaffney, H.D.Grimes, and C.Kang (2006).
Crystal structures of vegetative soybean lipoxygenase VLX-B and VLX-D, and comparisons with seed isoforms LOX-1 and LOX-3.
  Proteins, 65, 1008-1020.
PDB codes: 2iuj 2iuk
17029406 F.Wu, and B.J.Gaffney (2006).
Dynamic behavior of fatty acid spin labels within a binding site of soybean lipoxygenase-1.
  Biochemistry, 45, 12510-12518.  
16678271 H.Kühn, and V.B.O'Donnell (2006).
Inflammation and immune regulation by 12/15-lipoxygenases.
  Prog Lipid Res, 45, 334-356.  
16641090 M.Cristea, and E.H.Oliw (2006).
A G316A mutation of manganese lipoxygenase augments hydroperoxide isomerase activity: mechanism of biosynthesis of epoxyalcohols.
  J Biol Chem, 281, 17612-17623.  
16924104 M.Rakonjac, L.Fischer, P.Provost, O.Werz, D.Steinhilber, B.Samuelsson, and O.Rådmark (2006).
Coactosin-like protein supports 5-lipoxygenase enzyme activity and up-regulates leukotriene A4 production.
  Proc Natl Acad Sci U S A, 103, 13150-13155.  
16458008 M.Zheng, Z.Zhang, W.Zhu, H.Liu, X.Luo, K.Chen, and H.Jiang (2006).
Essential structural profile of a dual functional inhibitor against cyclooxygenase-2 (COX-2) and 5-lipoxygenase (5-LOX): molecular docking and 3D-QSAR analyses on DHDMBF analogues.
  Bioorg Med Chem, 14, 3428-3437.  
16755628 S.Herre, T.Schadendorf, I.Ivanov, C.Herrberger, W.Steinle, K.Rück-Braun, R.Preissner, and H.Kuhn (2006).
Photoactivation of an inhibitor of the 12/15-lipoxygenase pathway.
  Chembiochem, 7, 1089-1095.  
15778441 A.H.Pande, S.Qin, and S.A.Tatulian (2005).
Membrane fluidity is a key modulator of membrane binding, insertion, and activity of 5-lipoxygenase.
  Biophys J, 88, 4084-4094.  
15923196 C.Hörnig, D.Albert, L.Fischer, M.Hörnig, O.Rådmark, D.Steinhilber, and O.Werz (2005).
1-Oleoyl-2-acetylglycerol stimulates 5-lipoxygenase activity via a putative (phospho)lipid binding site within the N-terminal C2-like domain.
  J Biol Chem, 280, 26913-26921.  
15821095 D.P.Kloer, S.Ruch, S.Al-Babili, P.Beyer, and G.E.Schulz (2005).
The structure of a retinal-forming carotenoid oxygenase.
  Science, 308, 267-269.
PDB codes: 2biw 2bix
15951442 E.Poliakov, S.Gentleman, F.X.Cunningham, N.J.Miller-Ihli, and T.M.Redmond (2005).
Key role of conserved histidines in recombinant mouse beta-carotene 15,15'-monooxygenase-1 activity.
  J Biol Chem, 280, 29217-29223.  
16157595 G.Coffa, A.N.Imber, B.C.Maguire, G.Laxmikanthan, C.Schneider, B.J.Gaffney, and A.R.Brash (2005).
On the relationships of substrate orientation, hydrogen abstraction, and product stereochemistry in single and double dioxygenations by soybean lipoxygenase-1 and its Ala542Gly mutant.
  J Biol Chem, 280, 38756-38766.  
16270276 H.Kühn, I.Römisch, and J.Belkner (2005).
The role of lipoxygenase-isoforms in atherogenesis.
  Mol Nutr Food Res, 49, 1014-1029.  
16293000 H.Kuhn (2005).
Biologic relevance of lipoxygenase isoforms in atherogenesis.
  Expert Rev Cardiovasc Ther, 3, 1099-1110.  
16162493 M.L.Oldham, A.R.Brash, and M.E.Newcomer (2005).
Insights from the X-ray crystal structure of coral 8R-lipoxygenase: calcium activation via a C2-like domain and a structural basis of product chirality.
  J Biol Chem, 280, 39545-39552.
PDB code: 2fnq
16129665 S.Meruvu, M.Walther, I.Ivanov, S.Hammarström, G.Fürstenberger, P.Krieg, P.Reddanna, and H.Kuhn (2005).
Sequence determinants for the reaction specificity of murine (12R)-lipoxygenase: targeted substrate modification and site-directed mutagenesis.
  J Biol Chem, 280, 36633-36641.  
15799828 V.Subbarayan, X.C.Xu, J.Kim, P.Yang, A.Hoque, A.L.Sabichi, N.Llansa, G.Mendoza, C.J.Logothetis, R.A.Newman, S.M.Lippman, and D.G.Menter (2005).
Inverse relationship between 15-lipoxygenase-2 and PPAR-gamma gene expression in normal epithelia compared with tumor epithelia.
  Neoplasia, 7, 280-293.  
15584868 C.N.Serhan (2004).
Clues for new therapeutics in osteoporosis and periodontal disease: new roles for lipoxygenases?
  Expert Opin Ther Targets, 8, 643-652.  
15357019 E.H.Oliw, M.Cristea, and M.Hamberg (2004).
Biosynthesis and isomerization of 11-hydroperoxylinoleates by manganese- and iron-dependent lipoxygenases.
  Lipids, 39, 319-323.  
15496467 G.Coffa, and A.R.Brash (2004).
A single active site residue directs oxygenation stereospecificity in lipoxygenases: stereocontrol is linked to the position of oxygenation.
  Proc Natl Acad Sci U S A, 101, 15579-15584.  
15280375 M.Luo, S.M.Jones, S.M.Phare, M.J.Coffey, M.Peters-Golden, and T.G.Brock (2004).
Protein kinase A inhibits leukotriene synthesis by phosphorylation of 5-lipoxygenase on serine 523.
  J Biol Chem, 279, 41512-41520.  
14705020 O.Y.Borbulevych, J.Jankun, S.H.Selman, and E.Skrzypczak-Jankun (2004).
Lipoxygenase interactions with natural flavonoid, quercetin, reveal a complex with protocatechuic acid in its X-ray structure at 2.1 A resolution.
  Proteins, 54, 13-19.
PDB code: 1n8q
12543246 R.Wiesner, H.Suzuki, M.Walther, S.Yamamoto, and H.Kuhn (2003).
Suicidal inactivation of the rabbit 15-lipoxygenase by 15S-HpETE is paralleled by covalent modification of active site peptides.
  Free Radic Biol Med, 34, 304-315.  
12917400 S.Khanna, S.Roy, H.Ryu, P.Bahadduri, P.W.Swaan, R.R.Ratan, and C.K.Sen (2003).
Molecular basis of vitamin E action: tocotrienol modulates 12-lipoxygenase, a key mediator of glutamate-induced neurodegeneration.
  J Biol Chem, 278, 43508-43515.  
12525477 S.M.Jones, M.Luo, M.Peters-Golden, and T.G.Brock (2003).
Identification of two novel nuclear import sequences on the 5-lipoxygenase protein.
  J Biol Chem, 278, 10257-10263.  
12106812 H.Kühn, and A.Borchert (2002).
Regulation of enzymatic lipid peroxidation: the interplay of peroxidizing and peroxide reducing enzymes.
  Free Radic Biol Med, 33, 154-172.  
12221977 I.Feussner, and C.Wasternack (2002).
The lipoxygenase pathway.
  Annu Rev Plant Biol, 53, 275-297.  
12162745 J.S.Moody, and L.J.Marnett (2002).
Kinetics of inhibition of leukocyte 12-lipoxygenase by the isoform-specific inhibitor 4-(2-oxapentadeca-4-yne)phenylpropanoic acid.
  Biochemistry, 41, 10297-10303.  
12047377 L.Hörnsten, C.Su, A.E.Osbourn, U.Hellman, and E.H.Oliw (2002).
Cloning of the manganese lipoxygenase gene reveals homology with the lipoxygenase gene family.
  Eur J Biochem, 269, 2690-2697.  
12004065 M.Walther, M.Anton, M.Wiedmann, R.Fletterick, and H.Kuhn (2002).
The N-terminal domain of the reticulocyte-type 15-lipoxygenase is not essential for enzymatic activity but contains determinants for membrane binding.
  J Biol Chem, 277, 27360-27366.  
11796736 S.Kulkarni, S.Das, C.D.Funk, D.Murray, and W.Cho (2002).
Molecular basis of the specific subcellular localization of the C2-like domain of 5-lipoxygenase.
  J Biol Chem, 277, 13167-13174.  
11514227 M.Walther, I.Ivanov, G.Myagkova, and H.Kuhn (2001).
Alterations of lipoxygenase specificity by targeted substrate modification and site-directed mutagenesis.
  Chem Biol, 8, 779-790.  
11179969 R.K.Hughes, D.M.Lawson, A.R.Hornostaj, S.A.Fairhurst, and R.Casey (2001).
Mutagenesis and modelling of linoleate-binding to pea seed lipoxygenase.
  Eur J Biochem, 268, 1030-1040.  
11284695 R.Mogul, and T.R.Holman (2001).
Inhibition studies of soybean and human 15-lipoxygenases with long-chain alkenyl sulfate substrates.
  Biochemistry, 40, 4391-4397.  
11371199 T.Hammarberg, S.Kuprin, O.Rådmark, and A.Holmgren (2001).
EPR investigation of the active site of recombinant human 5-lipoxygenase: inhibition by selenide.
  Biochemistry, 40, 6371-6378.  
10672019 C.May, M.Höhne, P.Gnau, K.Schwennesen, and H.Kindl (2000).
The N-terminal beta-barrel structure of lipid body lipoxygenase mediates its binding to liposomes and lipid bodies.
  Eur J Biochem, 267, 1100-1109.  
11004552 E.Sudharshan, S.Srinivasulu, and A.G.Appu Rao (2000).
pH-induced domain interaction and conformational transitions of lipoxygenase-1.
  Biochim Biophys Acta, 1480, 13-22.  
11087405 K.Schwarz, C.Gerth, M.Anton, and H.Kuhn (2000).
Alterations in leukotriene synthase activity of the human 5-lipoxygenase by site-directed mutagenesis affecting its positional specificity.
  Biochemistry, 39, 14515-14521.  
10625675 M.Jisaka, R.B.Kim, W.E.Boeglin, and A.R.Brash (2000).
Identification of amino acid determinants of the positional specificity of mouse 8S-lipoxygenase and human 15S-lipoxygenase-2.
  J Biol Chem, 275, 1287-1293.  
10469604 A.Bateman, and R.Sandford (1999).
The PLAT domain: a new piece in the PKD1 puzzle.
  Curr Biol, 9, R588-R590.  
10446122 A.R.Brash (1999).
Lipoxygenases: occurrence, functions, catalysis, and acquisition of substrate.
  J Biol Chem, 274, 23679-23682.  
10607676 C.J.Schofield, and Z.Zhang (1999).
Structural and mechanistic studies on 2-oxoglutarate-dependent oxygenases and related enzymes.
  Curr Opin Struct Biol, 9, 722-731.  
10469603 C.P.Ponting, K.Hofmann, and P.Bork (1999).
A latrophilin/CL-1-like GPS domain in polycystin-1.
  Curr Biol, 9, R585-R588.  
16429610 C.R.Bowers, V.Storhaug, C.E.Webster, J.Bharatam, A.Cottone, R.Gianna, K.Betsey, and B.J.Gaffney (1999).
Exploring surfaces and cavities in lipoxygenase and other proteins by hyperpolarized xenon-129 NMR.
  J Am Chem Soc, 121, 9370-9377.  
10585402 E.Sudharshan, and A.G.Rao (1999).
Involvement of cysteine residues and domain interactions in the reversible unfolding of lipoxygenase-1.
  J Biol Chem, 274, 35351-35358.  
10570245 H.van Tilbeurgh, S.Bezzine, C.Cambillau, R.Verger, and F.Carrière (1999).
Colipase: structure and interaction with pancreatic lipase.
  Biochim Biophys Acta, 1441, 173-184.  
10542053 I.Kilty, A.Logan, and P.J.Vickers (1999).
Differential characteristics of human 15-lipoxygenase isozymes and a novel splice variant of 15S-lipoxygenase.
  Eur J Biochem, 266, 83-93.  
10464294 P.Christmas, J.W.Fox, S.R.Ursino, and R.J.Soberman (1999).
Differential localization of 5- and 15-lipoxygenases to the nuclear envelope in RAW macrophages.
  J Biol Chem, 274, 25594-25598.  
10436861 R.L.Bell, and R.R.Harris (1999).
The enzymology and pharmacology of 5-lipoxygenase and 5-lipoxygenase activating protein.
  Clin Rev Allergy Immunol, 17, 91.  
10601303 S.Borngräber, M.Browner, S.Gillmor, C.Gerth, M.Anton, R.Fletterick, and H.Kühn (1999).
Shape and specificity in mammalian 15-lipoxygenase active site. The functional interplay of sequence determinants for the reaction specificity.
  J Biol Chem, 274, 37345-37350.  
9922163 C.Pham, J.Jankun, E.Skrzypczak-Jankun, R.A.Flowers, and M.O.Funk (1998).
Structural and thermochemical characterization of lipoxygenase-catechol complexes.
  Biochemistry, 37, 17952-17957.
PDB code: 1byt
9837935 D.Sun, M.McDonnell, X.S.Chen, M.M.Lakkis, H.Li, S.N.Isaacs, S.H.Elsea, P.I.Patel, and C.D.Funk (1998).
Human 12(R)-lipoxygenase and the mouse ortholog. Molecular cloning, expression, and gene chromosomal assignment.
  J Biol Chem, 273, 33540-33547.  
9799493 K.Schwarz, S.Borngräber, M.Anton, and H.Kuhn (1998).
Probing the substrate alignment at the active site of 15-lipoxygenases by targeted substrate modification and site-directed mutagenesis. Evidence for an inverse substrate orientation.
  Biochemistry, 37, 15327-15335.  
9799511 S.A.Tatulian, J.Steczko, and W.Minor (1998).
Uncovering a calcium-regulated membrane-binding mechanism for soybean lipoxygenase-1.
  Biochemistry, 37, 15481-15490.  
9914255 U.Ermler, W.Grabarse, S.Shima, M.Goubeaud, and R.K.Thauer (1998).
Active sites of transition-metal enzymes with a focus on nickel.
  Curr Opin Struct Biol, 8, 749-758.  
9618483 W.E.Boeglin, R.B.Kim, and A.R.Brash (1998).
A 12R-lipoxygenase in human skin: mechanistic evidence, molecular cloning, and expression.
  Proc Natl Acad Sci U S A, 95, 6744-6749.  
9813031 X.S.Chen, Y.Y.Zhang, and C.D.Funk (1998).
Determinants of 5-lipoxygenase nuclear localization using green fluorescent protein/5-lipoxygenase fusion proteins.
  J Biol Chem, 273, 31237-31244.  
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