PDBsum entry 1k81

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protein metals links
Translation PDB id
Protein chain
36 a.a. *
* Residue conservation analysis
PDB id:
Name: Translation
Title: Nmr structure of the zinc-ribbon domain within translation initiation factor 2 subunit beta
Structure: Probable translation initiation factor 2 beta subunit. Chain: a. Synonym: eif-2-beta. Engineered: yes
Source: Methanocaldococcus jannaschii. Organism_taxid: 2190. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
NMR struc: 10 models
Authors: S.Cho,D.W.Hoffman
Key ref:
S.Cho and D.W.Hoffman (2002). Structure of the beta subunit of translation initiation factor 2 from the archaeon Methanococcus jannaschii: a representative of the eIF2beta/eIF5 family of proteins. Biochemistry, 41, 5730-5742. PubMed id: 11980477 DOI: 10.1021/bi011984n
22-Oct-01     Release date:   24-Apr-02    
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Protein chain
Pfam   ArchSchema ?
Q57562  (IF2B_METJA) -  Translation initiation factor 2 subunit beta
143 a.a.
36 a.a.
Key:    PfamA domain  Secondary structure

 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     translational initiation   1 term 
  Biochemical function     translation initiation factor activity     1 term  


DOI no: 10.1021/bi011984n Biochemistry 41:5730-5742 (2002)
PubMed id: 11980477  
Structure of the beta subunit of translation initiation factor 2 from the archaeon Methanococcus jannaschii: a representative of the eIF2beta/eIF5 family of proteins.
S.Cho, D.W.Hoffman.
The beta subunit of archaeal translation initiation factor 2 (aIF2beta) is a representative of a family of proteins whose members include the beta subunit of eukaryotic translation initiation factor 2 (eIF2beta) and the N-terminal domain within translation initiation factor 5 (eIF5); no members of this family of proteins have been structurally characterized up to this time. In the work presented here, aIF2beta from Methanococcus jannaschii was expressed in Escherichia coli, purified, and analyzed using multidimensional NMR methods. The aIF2beta was found to contain two independent structural domains. The N-terminal domain contains a four-stranded antiparallel beta sheet and two alpha helices, and is structurally similar to the DNA-binding domain of a yeast heat shock transcription factor and a domain within ribosomal protein S4. This structural similarity was an unanticipated result, since no significant homology was detected at the level of primary sequence. The C-terminal domain of aIF2beta contains a zinc-binding motif of three antiparallel beta strands, with four conserved cysteines arranged as two CXXC units separated by 17 residues. Conserved residues on the surface of each domain that are likely candidates for direct interaction with other components of the translational apparatus were identified. The significant primary sequence homology between archaeal aIF2beta and the eukaryotic eIF2beta and eIF5, when combined with the structural results in the work presented here, permitted structural features to be predicted for these latter two eukaryotic proteins.

Literature references that cite this PDB file's key reference

  PubMed id Reference
21568863 E.A.Stolboushkina, and M.B.Garber (2011).
Eukaryotic type translation initiation factor 2: structure-functional aspects.
  Biochemistry (Mosc), 76, 283-294.  
19703275 A.Y.Mulkidjanian, and M.Y.Galperin (2009).
On the origin of life in the Zinc world. 2. Validation of the hypothesis on the photosynthesizing zinc sulfide edifices as cradles of life on Earth.
  Biol Direct, 4, 27.  
19548767 T.Bereau, and M.Deserno (2009).
Generic coarse-grained model for protein folding and aggregation.
  J Chem Phys, 130, 235106.  
18000047 L.Yatime, Y.Mechulam, S.Blanquet, and E.Schmitt (2007).
Structure of an archaeal heterotrimeric initiation factor 2 reveals a nucleotide state between the GTP and the GDP states.
  Proc Natl Acad Sci U S A, 104, 18445-18450.
PDB codes: 2qmu 2qn6
16924118 M.Sokabe, M.Yao, N.Sakai, S.Toya, and I.Tanaka (2006).
Structure of archaeal translational initiation factor 2 betagamma-GDP reveals significant conformational change of the beta-subunit and switch 1 region.
  Proc Natl Acad Sci U S A, 103, 13016-13021.
PDB codes: 2d74 2dcu
14978306 P.Gutiérrez, M.J.Osborne, N.Siddiqui, J.F.Trempe, C.Arrowsmith, and K.Gehring (2004).
Structure of the archaeal translation initiation factor aIF2 beta from Methanobacterium thermoautotrophicum: implications for translation initiation.
  Protein Sci, 13, 659-667.
PDB code: 1nee
12581660 N.Sonenberg, and T.E.Dever (2003).
Eukaryotic translation initiation factors and regulators.
  Curr Opin Struct Biol, 13, 56-63.  
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