PDBsum entry 1k1z

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protein links
Signaling protein PDB id
Protein chain
78 a.a. *
* Residue conservation analysis
PDB id:
Name: Signaling protein
Title: Solution structure of n-terminal sh3 domain mutant(p33g) of murine vav
Structure: Vav. Chain: a. Fragment: n-terminal sh3 domain. Synonym: vav proto-oncogene. Engineered: yes. Mutation: yes
Source: Mus musculus. House mouse. Organism_taxid: 10090. Expressed in: escherichia coli. Expression_system_taxid: 562.
NMR struc: 1 models
Authors: K.Ogura,K.Nagata,M.Horiuchi,E.Ebisui,T.Hasuda,S.Yuzawa, M.Nishida,H.Hatanaka,F.Inagaki
Key ref: K.Ogura et al. (2002). Solution structure of N-terminal SH3 domain of Vav and the recognition site for Grb2 C-terminal SH3 domain. J Biomol NMR, 22, 37-46. PubMed id: 11885979
26-Sep-01     Release date:   10-Oct-01    
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Protein chain
Pfam   ArchSchema ?
P27870  (VAV_MOUSE) -  Proto-oncogene vav
845 a.a.
78 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     small GTPase mediated signal transduction   1 term 


J Biomol NMR 22:37-46 (2002)
PubMed id: 11885979  
Solution structure of N-terminal SH3 domain of Vav and the recognition site for Grb2 C-terminal SH3 domain.
K.Ogura, K.Nagata, M.Horiuchi, E.Ebisui, T.Hasuda, S.Yuzawa, M.Nishida, H.Hatanaka, F.Inagaki.
The three-dimensional structure of the N-terminal SH3 domain (residues 583-660) of murine Vav, which contains a tetra-proline sequence (Pro 607-Pro 610), was determined by NMR. The solution structure of the SH3 domain shows a typical SH3 fold, but it exists in two conformations due to cis-trans isomerization at the Gly614-Pro615 bond. The NMR structure of the P615G mutant, where Pro615 is replaced by glycine, reveals that the tetra-proline region is inserted into the RT-loop and binds to its own SH3 structure. The C-terminal SH3 domain of Grb2 specifically binds to the trans form of the N-terminal SH3 domain of Vav. The surface of Vav N-terminal SH3 which binds to Grb2 C-terminal SH3 was elucidated by chemical shift mapping experiments using NMR. The surface does not involve the tetra-proline region but involves the region comprising the n-src loop, the N-terminal and the C-terminal regions. This surface is located opposite to the tetra-proline containing region, consistent with that of our previous mutagenesis studies.

Literature references that cite this PDB file's key reference

  PubMed id Reference
20208353 K.Nagata (2010).
Studies of the structure-activity relationships of peptides and proteins involved in growth and development based on their three-dimensional structures.
  Biosci Biotechnol Biochem, 74, 462-470.  
17894348 I.Hudáky, and A.Perczel (2008).
Prolylproline unit in model peptides and in fragments from databases.
  Proteins, 70, 1389-1407.  
17923698 E.Giurisato, M.Cella, T.Takai, T.Kurosaki, Y.Feng, G.D.Longmore, M.Colonna, and A.S.Shaw (2007).
Phosphatidylinositol 3-kinase activation is required to form the NKG2D immunological synapse.
  Mol Cell Biol, 27, 8583-8599.  
17050525 A.V.Miletic, K.Sakata-Sogawa, M.Hiroshima, M.J.Hamann, T.S.Gomez, N.Ota, T.Kloeppel, O.Kanagawa, M.Tokunaga, D.D.Billadeau, and W.Swat (2006).
Vav1 acidic region tyrosine 174 is required for the formation of T cell receptor-induced microclusters and is essential in T cell development and activation.
  J Biol Chem, 281, 38257-38265.  
16582911 J.L.Upshaw, L.N.Arneson, R.A.Schoon, C.J.Dick, D.D.Billadeau, and P.J.Leibson (2006).
NKG2D-mediated signaling requires a DAP10-bound Grb2-Vav1 intermediate and phosphatidylinositol-3-kinase in human natural killer cells.
  Nat Immunol, 7, 524-532.  
16644733 M.R.Schiller, K.Chakrabarti, G.F.King, N.I.Schiller, B.A.Eipper, and M.W.Maciejewski (2006).
Regulation of RhoGEF activity by intramolecular and intermolecular SH3 domain interactions.
  J Biol Chem, 281, 18774-18786.
PDB code: 1u3o
12773374 M.Harkiolaki, M.Lewitzky, R.J.Gilbert, E.Y.Jones, R.P.Bourette, G.Mouchiroud, H.Sondermann, I.Moarefi, and S.M.Feller (2003).
Structural basis for SH3 domain-mediated high-affinity binding between Mona/Gads and SLP-76.
  EMBO J, 22, 2571-2582.
PDB code: 1oeb
14523014 Q.Cui, Y.Tong, H.Xue, L.Huang, Y.Feng, and J.Wang (2003).
Two conformations of archaeal Ssh10b. The origin of its temperature-dependent interaction with DNA.
  J Biol Chem, 278, 51015-51022.  
12670394 V.L.Tybulewicz, L.Ardouin, A.Prisco, and L.F.Reynolds (2003).
Vav1: a key signal transducer downstream of the TCR.
  Immunol Rev, 192, 42-52.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.