PDBsum entry 1jsa

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Calcium binding PDB id
Protein chain
188 a.a. *
_CA ×2
* Residue conservation analysis
PDB id:
Name: Calcium binding
Title: Myristoylated recoverin with two calciums bound, nmr, 24 structures
Structure: Recoverin. Chain: a. Engineered: yes
Source: Bos taurus. Cattle. Organism_taxid: 9913. Expressed in: escherichia coli. Expression_system_taxid: 562.
NMR struc: 24 models
Authors: J.B.Ames,R.Ishima,T.Tanaka,J.I.Gordon,L.Stryer,M.Ikura
Key ref:
J.B.Ames et al. (1997). Molecular mechanics of calcium-myristoyl switches. Nature, 389, 198-202. PubMed id: 9296500 DOI: 10.1038/38310
04-Jun-97     Release date:   15-Oct-97    
Go to PROCHECK summary

Protein chain
Pfam   ArchSchema ?
P21457  (RECO_BOVIN) -  Recoverin
202 a.a.
188 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     cytosol   1 term 
  Biological process     response to stimulus   4 terms 
  Biochemical function     protein binding     3 terms  


DOI no: 10.1038/38310 Nature 389:198-202 (1997)
PubMed id: 9296500  
Molecular mechanics of calcium-myristoyl switches.
J.B.Ames, R.Ishima, T.Tanaka, J.I.Gordon, L.Stryer, M.Ikura.
Many eukaryotic cellular and viral proteins have a covalently attached myristoyl group at the amino terminus. One such protein is recoverin, a calcium sensor in retinal rod cells, which controls the lifetime of photoexcited rhodopsin by inhibiting rhodopsin kinase. Recoverin has a relative molecular mass of 23,000 23K), and contains an amino-terminal myristoyl group (or related acyl group) and four EF hands. The binding of two Ca2+ ions to recoverin leads to its translocation from the cytosol to the disc membrane. In the Ca2+-free state, the myristoyl group is sequestered in a deep hydrophobic box, where it is clamped by multiple residues contributed by three of the EF hands. We have used nuclear magnetic resonance to show that Ca2+ induces the unclamping and extrusion of the myristoyl group, enabling it to interact with a lipid bilayer membrane. The transition is also accompanied by a 45-degree rotation of the amino-terminal domain relative to the carboxy-terminal domain, and many hydrophobic residues are exposed. The conservation of the myristoyl binding site and two swivels in recoverin homologues from yeast to humans indicates that calcium-myristoyl switches are ancient devices for controlling calcium-sensitive processes.
  Selected figure(s)  
Figure 2.
Figure 2 Space-filling model (a) and ribbon diagram (b) of Ca^2+-free^10 (left, liku.pdb) and Ca^2+-bound (right) myristoylated recoverin. The C-terminal domains of the two forms are aligned to show the Ca^2+-induced 45-deg rotation of the N-terminal domain.
Figure 4.
Figure 4 Schematic ribbon representation of the structure of the N-terminal domain of Ca^2+-free (top) and Ca^2+-bound (bottom) recoverin. Rotation about Gly 42 enables the extrusion of the myristoyl group.
  The above figures are reprinted by permission from Macmillan Publishers Ltd: Nature (1997, 389, 198-202) copyright 1997.  
  Figures were selected by an automated process.  

Literature references that cite this PDB file's key reference

  PubMed id Reference
21568868 I.I.Senin, N.K.Tikhomirova, V.A.Churumova, I.I.Grigoriev, T.A.Kolpakova, D.V.Zinchenko, P.P.Philippov, and E.Y.Zernii (2011).
Amino Acid sequences of two immune-dominant epitopes of recoverin are involved in ca2+/recoverin-dependent inhibition of phosphorylation of rhodopsin.
  Biochemistry (Mosc), 76, 332-338.  
20857168 K.Chandra, V.Ramakrishnan, Y.Sharma, and K.V.Chary (2011).
N-terminal myristoylation alters the calcium binding pathways in neuronal calcium sensor-1.
  J Biol Inorg Chem, 16, 81-95.  
21442718 M.Nullmeier, H.Koliwer-Brandl, S.Kelm, P.Zägel, K.W.Koch, and I.Brand (2011).
Impact of strong and weak lipid-protein interactions on the structure of a lipid bilayer on a gold electrode surface.
  Chemphyschem, 12, 1066-1079.  
21327964 S.Theisgen, L.Thomas, T.Schröder, C.Lange, M.Kovermann, J.Balbach, and D.Huster (2011).
The presence of membranes or micelles induces structural changes of the myristoylated guanylate-cyclase activating protein-2.
  Eur Biophys J, 40, 565-576.  
  21465563 X.Xu, R.Ishima, and J.B.Ames (2011).
Conformational dynamics of recoverin's Ca(2+) -myristoyl switch probed by (15) N NMR relaxation dispersion and chemical shift analysis.
  Proteins, 79, 1910-1922.  
20704590 A.V.Dovgan, V.P.Cherkas, A.R.Stepanyuk, D.J.Fitzgerald, L.P.Haynes, A.V.Tepikin, R.D.Burgoyne, and P.V.Belan (2010).
Decoding glutamate receptor activation by the Ca2+ sensor protein hippocalcin in rat hippocampal neurons.
  Eur J Neurosci, 32, 347-358.  
20835460 D.Dell'Orco, M.Müller, and K.W.Koch (2010).
Quantitative detection of conformational transitions in a calcium sensor protein by surface plasmon resonance.
  Chem Commun (Camb), 46, 7316-7318.  
20662770 D.I.Chan, and H.J.Vogel (2010).
Current understanding of fatty acid biosynthesis and the acyl carrier protein.
  Biochem J, 430, 1.  
20096337 G.Joshi, C.D.Aluise, M.P.Cole, R.Sultana, W.M.Pierce, M.Vore, D.K.St Clair, and D.A.Butterfield (2010).
Alterations in brain antioxidant enzymes and redox proteomic identification of oxidized brain proteins induced by the anti-cancer drug adriamycin: implications for oxidative stress-mediated chemobrain.
  Neuroscience, 166, 796-807.  
19935658 H.Gerlach, V.Laumann, S.Martens, C.F.Becker, R.S.Goody, and M.Geyer (2010).
HIV-1 Nef membrane association depends on charge, curvature, composition and sequence.
  Nat Chem Biol, 6, 46-53.  
20404920 J.M.Glück, S.Hoffmann, B.W.Koenig, and D.Willbold (2010).
Single vector system for efficient N-myristoylation of recombinant proteins in E. coli.
  PLoS One, 5, e10081.  
20974912 J.S.Oakhill, Z.P.Chen, J.W.Scott, R.Steel, L.A.Castelli, N.Ling, S.L.Macaulay, and B.E.Kemp (2010).
β-Subunit myristoylation is the gatekeeper for initiating metabolic stress sensing by AMP-activated protein kinase (AMPK).
  Proc Natl Acad Sci U S A, 107, 19237-19241.  
19854831 K.E.Jarman, P.A.Moretti, J.R.Zebol, and S.M.Pitson (2010).
Translocation of sphingosine kinase 1 to the plasma membrane is mediated by calcium- and integrin-binding protein 1.
  J Biol Chem, 285, 483-492.  
20152148 K.G.Valentine, R.W.Peterson, J.S.Saad, M.F.Summers, X.Xu, J.B.Ames, and A.J.Wand (2010).
Reverse micelle encapsulation of membrane-anchored proteins for solution NMR studies.
  Structure, 18, 9.  
21097705 M.T.Smith, J.Meissner, S.Esmonde, H.J.Wong, and E.M.Meiering (2010).
Energetics and mechanisms of folding and flipping the myristoyl switch in the {beta}-trefoil protein, hisactophilin.
  Proc Natl Acad Sci U S A, 107, 20952-20957.  
  20948784 R.D.Burgoyne, and L.P.Haynes (2010).
Neuronal calcium sensor proteins: emerging roles in membrane traffic and synaptic plasticity.
  F1000 Biol Rep, 2, 0.  
20152158 T.Orban, G.Bereta, M.Miyagi, B.Wang, M.R.Chance, M.C.Sousa, and K.Palczewski (2010).
Conformational changes in guanylate cyclase-activating protein 1 induced by Ca2+ and N-terminal fatty acid acylation.
  Structure, 18, 116-126.  
19063970 C.J.Zhao, C.Noack, M.Brackmann, T.Gloveli, A.Maelicke, U.Heinemann, R.Anand, and K.H.Braunewell (2009).
Neuronal Ca2+ sensor VILIP-1 leads to the upregulation of functional alpha4beta2 nicotinic acetylcholine receptors in hippocampal neurons.
  Mol Cell Neurosci, 40, 280-292.  
18925431 C.Zhao, R.Anand, and K.H.Braunewell (2009).
Nicotine-induced Ca2+-myristoyl switch of neuronal Ca2+ sensor VILIP-1 in hippocampal neurons: a possible crosstalk mechanism for nicotinic receptors.
  Cell Mol Neurobiol, 29, 273-286.  
19413971 H.A.Scheidt, and D.Huster (2009).
Structure and dynamics of the myristoyl lipid modification of SRC peptides determined by 2H solid-state NMR spectroscopy.
  Biophys J, 96, 3663-3672.  
18942727 J.L.Li, C.Y.Geng, Y.Bu, X.R.Huang, and C.C.Sun (2009).
Conformational transition pathway in the allosteric process of calcium-induced recoverin: molecular dynamics simulations.
  J Comput Chem, 30, 1135-1145.  
18989702 K.H.Braunewell, and A.J.Szanto (2009).
Visinin-like proteins (VSNLs): interaction partners and emerging functions in signal transduction of a subfamily of neuronal Ca2+ -sensor proteins.
  Cell Tissue Res, 335, 301-316.  
19720824 S.Dobney, D.Chiasson, P.Lam, S.P.Smith, and W.A.Snedden (2009).
The calmodulin-related calcium sensor CML42 plays a role in trichome branching.
  J Biol Chem, 284, 31647-31657.  
19143494 S.Lim, I.Peshenko, A.Dizhoor, and J.B.Ames (2009).
Effects of Ca2+, Mg2+, and myristoylation on guanylyl cyclase activating protein 1 structure and stability.
  Biochemistry, 48, 850-862.  
19851889 S.Lim, and J.B.Ames (2009).
(1)H, (15)N, and (13)C chemical shift assignments of neuronal calcium sensor-1 homolog from fission yeast.
  Biomol NMR Assign, 3, 269-271.  
19815554 Y.Han, E.Eppinger, I.G.Schuster, L.U.Weigand, X.Liang, E.Kremmer, C.Peschel, and A.M.Krackhardt (2009).
Formin-like 1 (FMNL1) is regulated by N-terminal myristoylation and induces polarized membrane blebbing.
  J Biol Chem, 284, 33409-33417.  
19550036 Y.S.Liao, K.C.Chen, and L.S.Chang (2009).
Functional role of EF-hands 3 and 4 in membrane-binding of KChIP1.
  J Biosci, 34, 203-211.  
18266817 A.Torisawa, D.Arinobu, S.Tachibanaki, and S.Kawamura (2008).
Amino acid residues in GRK1/GRK7 responsible for interaction with S-modulin/recoverin.
  Photochem Photobiol, 84, 823-830.  
17763944 B.R.Souza, B.S.Motta, D.V.Rosa, K.C.Torres, A.A.Castro, C.M.Comim, A.M.Sampaio, F.F.Lima, A.Jeromin, J.Quevedo, and M.A.Romano-Silva (2008).
DARPP-32 and NCS-1 Expression is not Altered in Brains of Rats Treated with Typical or Atypical Antipsychotics.
  Neurochem Res, 33, 533-538.  
18280495 E.Johnson, L.Bruschweiler-Li, S.A.Showalter, G.W.Vuister, F.Zhang, and R.Brüschweiler (2008).
Structure and dynamics of Ca2+-binding domain 1 of the Na+/Ca2+ exchanger in the presence and in the absence of Ca2+.
  J Mol Biol, 377, 945-955.  
18559337 J.N.Wingard, J.Ladner, M.Vanarotti, A.J.Fisher, H.Robinson, K.T.Buchanan, D.M.Engman, and J.B.Ames (2008).
Structural Insights into Membrane Targeting by the Flagellar Calcium-binding Protein (FCaBP), a Myristoylated and Palmitoylated Calcium Sensor in Trypanosoma cruzi.
  J Biol Chem, 283, 23388-23396.
PDB code: 3cs1
18202676 L.P.Haynes, and R.D.Burgoyne (2008).
Unexpected tails of a Ca2+ sensor.
  Nat Chem Biol, 4, 90-91.  
18786382 M.J.Boulware, and J.S.Marchant (2008).
Timing in cellular Ca2+ signaling.
  Curr Biol, 18, R769-R776.  
18634855 O.Markova, D.Fitzgerald, A.Stepanyuk, A.Dovgan, V.Cherkas, A.Tepikin, R.D.Burgoyne, and P.Belan (2008).
Hippocalcin signaling via site-specific translocation in hippocampal neurons.
  Neurosci Lett, 442, 152-157.  
18346093 R.Stephen, S.Filipek, K.Palczewski, and M.C.Sousa (2008).
Ca2+ -dependent regulation of phototransduction.
  Photochem Photobiol, 84, 903-910.  
17596311 E.Stansell, R.Apkarian, S.Haubova, W.E.Diehl, E.M.Tytler, and E.Hunter (2007).
Basic residues in the Mason-Pfizer monkey virus gag matrix domain regulate intracellular trafficking and capsid-membrane interactions.
  J Virol, 81, 8977-8988.  
18034895 I.I.Senin, V.A.Churumova, P.P.Philippov, and K.W.Koch (2007).
Membrane binding of the neuronal calcium sensor recoverin - modulatory role of the charged carboxy-terminus.
  BMC Biochem, 8, 24.  
17545152 I.V.Peshenko, and A.M.Dizhoor (2007).
Activation and inhibition of photoreceptor guanylyl cyclase by guanylyl cyclase activating protein 1 (GCAP-1): the functional role of Mg2+/Ca2+ exchange in EF-hand domains.
  J Biol Chem, 282, 21645-21652.  
17355250 J.Burré, and W.Volknandt (2007).
The synaptic vesicle proteome.
  J Neurochem, 101, 1448-1462.  
17526567 P.Desmeules, S.E.Penney, B.Desbat, and C.Salesse (2007).
Determination of the contribution of the myristoyl group and hydrophobic amino acids of recoverin on its dynamics of binding to lipid monolayers.
  Biophys J, 93, 2069-2082.  
17997965 R.Stephen, G.Bereta, M.Golczak, K.Palczewski, and M.C.Sousa (2007).
Stabilizing function for myristoyl group revealed by the crystal structure of a neuronal calcium sensor, guanylate cyclase-activating protein 1.
  Structure, 15, 1392-1402.
PDB code: 2r2i
17078090 T.Gensch, K.E.Komolov, I.I.Senin, P.P.Philippov, and K.W.Koch (2007).
Ca2+-dependent conformational changes in the neuronal Ca2+-sensor recoverin probed by the fluorescent dye Alexa647.
  Proteins, 66, 492-499.  
17720810 T.Strahl, I.G.Huttner, J.D.Lusin, M.Osawa, D.King, J.Thorner, and J.B.Ames (2007).
Structural insights into activation of phosphatidylinositol 4-kinase (Pik1) by yeast frequenin (Frq1).
  J Biol Chem, 282, 30949-30959.
PDB code: 2ju0
16765896 E.Babini, I.Bertini, F.Capozzi, E.Chirivino, and C.Luchinat (2006).
A structural and dynamic characterization of the EF-hand protein CLSP.
  Structure, 14, 1029-1038.
PDB code: 2b1u
16733800 E.Fik-Rymarkiewicz, T.Duda, and R.K.Sharma (2006).
Novel frequenin-modulated Ca2+-signaling membrane guanylate cyclase (ROS-GC) transduction pathway in bovine hippocampus.
  Mol Cell Biochem, 291, 187-204.  
16847255 E.O.Freed (2006).
HIV-1 Gag: flipped out for PI(4,5)P(2).
  Proc Natl Acad Sci U S A, 103, 11101-11102.  
16731532 F.F.Dai, Y.Zhang, Y.Kang, Q.Wang, H.Y.Gaisano, K.H.Braunewell, C.B.Chan, and M.B.Wheeler (2006).
The neuronal Ca2+ sensor protein visinin-like protein-1 is expressed in pancreatic islets and regulates insulin secretion.
  J Biol Chem, 281, 21942-21953.  
17020884 J.B.Ames, K.Levay, J.N.Wingard, J.D.Lusin, and V.Z.Slepak (2006).
Structural basis for calcium-induced inhibition of rhodopsin kinase by recoverin.
  J Biol Chem, 281, 37237-37245.
PDB code: 2i94
16638749 Barry, A.Janoshazi, J.L.Dupont, O.Procksch, S.Chasserot-Golaz, A.Jeromin, and N.Vitale (2006).
Functional implication of neuronal calcium sensor-1 and phosphoinositol 4-kinase-beta interaction in regulated exocytosis of PC12 cells.
  J Biol Chem, 281, 18098-18111.  
16432210 M.Ikura, and J.B.Ames (2006).
Genetic polymorphism and protein conformational plasticity in the calmodulin superfamily: two ways to promote multifunctionality.
  Proc Natl Acad Sci U S A, 103, 1159-1164.  
17015448 O.H.Weiergräber, I.I.Senin, E.Y.Zernii, V.A.Churumova, N.A.Kovaleva, A.A.Nazipova, S.E.Permyakov, E.A.Permyakov, P.P.Philippov, J.Granzin, and K.W.Koch (2006).
Tuning of a neuronal calcium sensor.
  J Biol Chem, 281, 37594-37602.
PDB code: 2het
16710297 Y.B.Ammar, S.Takeda, T.Hisamitsu, H.Mori, and S.Wakabayashi (2006).
Crystal structure of CHP2 complexed with NHE1-cytosolic region and an implication for pH regulation.
  EMBO J, 25, 2315-2325.
PDB code: 2bec
15898063 B.Blasiole, N.Kabbani, W.Boehmler, B.Thisse, C.Thisse, V.Canfield, and R.Levenson (2005).
Neuronal calcium sensor-1 gene ncs-1a is essential for semicircular canal formation in zebrafish inner ear.
  J Neurobiol, 64, 285-297.  
15699030 D.Muralidhar, M.K.Jobby, K.Krishnan, V.Annapurna, K.V.Chary, A.Jeromin, and Y.Sharma (2005).
Equilibrium unfolding of neuronal calcium sensor-1: N-terminal myristoylation influences unfolding and reduces protein stiffening in the presence of calcium.
  J Biol Chem, 280, 15569-15578.  
15574431 H.R.Gentry, A.U.Singer, L.Betts, C.Yang, J.D.Ferrara, J.Sondek, and L.V.Parise (2005).
Structural and biochemical characterization of CIB1 delineates a new family of EF-hand-containing proteins.
  J Biol Chem, 280, 8407-8415.
PDB code: 1xo5
16147998 J.N.Wingard, J.Chan, I.Bosanac, F.Haeseleer, K.Palczewski, M.Ikura, and J.B.Ames (2005).
Structural analysis of Mg2+ and Ca2+ binding to CaBP1, a neuron-specific regulator of calcium channels.
  J Biol Chem, 280, 37461-37470.  
15843174 K.E.Komolov, D.V.Zinchenko, V.A.Churumova, S.A.Vaganova, O.H.Weiergräber, I.I.Senin, P.P.Philippov, and K.W.Koch (2005).
One of the Ca2+ binding sites of recoverin exclusively controls interaction with rhodopsin kinase.
  Biol Chem, 386, 285-289.  
16148003 K.T.Buchanan, J.B.Ames, S.H.Asfaw, J.N.Wingard, C.L.Olson, P.T.Campana, A.P.Araújo, and D.M.Engman (2005).
A flagellum-specific calcium sensor.
  J Biol Chem, 280, 40104-40111.  
16043511 M.Fivaz, and T.Meyer (2005).
Reversible intracellular translocation of KRas but not HRas in hippocampal neurons regulated by Ca2+/calmodulin.
  J Cell Biol, 170, 429-441.  
16212700 R.D.Hamer, S.C.Nicholas, D.Tranchina, T.D.Lamb, and J.L.Jarvinen (2005).
Toward a unified model of vertebrate rod phototransduction.
  Vis Neurosci, 22, 417-436.  
15858006 S.Dawe, J.A.Corcoran, E.K.Clancy, J.Salsman, and R.Duncan (2005).
Unusual topological arrangement of structural motifs in the baboon reovirus fusion-associated small transmembrane protein.
  J Virol, 79, 6216-6226.  
15987692 Y.Naoe, K.Arita, H.Hashimoto, H.Kanazawa, M.Sato, and T.Shimizu (2005).
Structural characterization of calcineurin B homologous protein 1.
  J Biol Chem, 280, 32372-32378.
PDB code: 2ct9
15102861 A.Jeromin, D.Muralidhar, M.N.Parameswaran, J.Roder, T.Fairwell, S.Scarlata, L.Dowal, S.M.Mustafi, K.V.Chary, and Y.Sharma (2004).
N-terminal myristoylation regulates calcium-induced conformational changes in neuronal calcium sensor-1.
  J Biol Chem, 279, 27158-27167.  
14699046 C.Tang, E.Loeliger, P.Luncsford, I.Kinde, D.Beckett, and M.F.Summers (2004).
Entropic switch regulates myristate exposure in the HIV-1 matrix protein.
  Proc Natl Acad Sci U S A, 101, 517-522.
PDB code: 1uph
14726528 D.W.O'Callaghan, and R.D.Burgoyne (2004).
Identification of residues that determine the absence of a Ca(2+)/myristoyl switch in neuronal calcium sensor-1.
  J Biol Chem, 279, 14347-14354.  
15113883 E.Stansell, E.Tytler, M.R.Walter, and E.Hunter (2004).
An early stage of Mason-Pfizer monkey virus budding is regulated by the hydrophobicity of the Gag matrix domain core.
  J Virol, 78, 5023-5031.  
15448139 I.V.Peshenko, E.V.Olshevskaya, and A.M.Dizhoor (2004).
Ca(2+)-dependent conformational changes in guanylyl cyclase-activating protein 2 (GCAP-2) revealed by site-specific phosphorylation and partial proteolysis.
  J Biol Chem, 279, 50342-50349.  
14657246 J.Andrade, H.Zhao, B.Titus, S.Timm Pearce, and M.Barroso (2004).
The EF-hand Ca2+-binding protein p22 plays a role in microtubule and endoplasmic reticulum organization and dynamics with distinct Ca2+-binding requirements.
  Mol Biol Cell, 15, 481-496.  
14739275 K.Oikawa, S.Kimura, N.Aoki, Y.Atsuta, Y.Takiyama, T.Nagato, M.Yanai, H.Kobayashi, K.Sato, T.Sasajima, and M.Tateno (2004).
Neuronal calcium sensor protein visinin-like protein-3 interacts with microsomal cytochrome b5 in a Ca2+-dependent manner.
  J Biol Chem, 279, 15142-15152.  
14707265 M.D.Resh (2004).
A myristoyl switch regulates membrane binding of HIV-1 Gag.
  Proc Natl Acad Sci U S A, 101, 417-418.  
14765114 M.Matsubara, T.Nakatsu, H.Kato, and H.Taniguchi (2004).
Crystal structure of a myristoylated CAP-23/NAP-22 N-terminal domain complexed with Ca2+/calmodulin.
  EMBO J, 23, 712-718.
PDB code: 1l7z
14722091 N.Hamasaki-Katagiri, T.Molchanova, K.Takeda, and J.B.Ames (2004).
Fission yeast homolog of neuronal calcium sensor-1 (Ncs1p) regulates sporulation and confers calcium tolerance.
  J Biol Chem, 279, 12744-12754.  
15627391 N.K.Tikhomirova, M.A.Goncharskaya, and I.I.Senin (2004).
Preparation and characteristics of Ca(2+)-dependent monoclonal antibodies to recoverin.
  Biochemistry (Mosc), 69, 1360-1364.  
15046879 R.D.Burgoyne, D.W.O'Callaghan, B.Hasdemir, L.P.Haynes, and A.V.Tepikin (2004).
Neuronal Ca2+-sensor proteins: multitalented regulators of neuronal function.
  Trends Neurosci, 27, 203-209.  
14980206 W.Zhou, Y.Qian, K.Kunjilwar, P.J.Pfaffinger, and S.Choe (2004).
Structural insights into the functional interaction of KChIP1 with Shal-type K(+) channels.
  Neuron, 41, 573-586.
PDB code: 1s6c
14638856 D.W.O'Callaghan, A.V.Tepikin, and R.D.Burgoyne (2003).
Dynamics and calcium sensitivity of the Ca2+/myristoyl switch protein hippocalcin in living cells.
  J Cell Biol, 163, 715-721.  
  14739996 I.Sokal, A.Alekseev, and K.Palczewski (2003).
Photoreceptor guanylate cyclase variants: cGMP production under control.
  Acta Biochim Pol, 50, 1075-1095.  
13678959 K.D.Ridge, N.G.Abdulaev, M.Sousa, and K.Palczewski (2003).
Phototransduction: crystal clear.
  Trends Biochem Sci, 28, 479-487.  
14506265 M.Matsubara, K.Titani, H.Taniguchi, and N.Hayashi (2003).
Direct involvement of protein myristoylation in myristoylated alanine-rich C kinase substrate (MARCKS)-calmodulin interaction.
  J Biol Chem, 278, 48898-48902.  
12871972 M.Nagae, A.Nozawa, N.Koizumi, H.Sano, H.Hashimoto, M.Sato, and T.Shimizu (2003).
The crystal structure of the novel calcium-binding protein AtCBL2 from Arabidopsis thaliana.
  J Biol Chem, 278, 42240-42246.
PDB code: 1uhn
12471042 M.Rajebhosale, S.Greenwood, J.Vidugiriene, A.Jeromin, and S.Hilfiker (2003).
Phosphatidylinositol 4-OH kinase is a downstream target of neuronal calcium sensor-1 in enhancing exocytosis in neuroendocrine cells.
  J Biol Chem, 278, 6075-6084.  
12686556 O.H.Weiergräber, I.I.Senin, P.P.Philippov, J.Granzin, and K.W.Koch (2003).
Impact of N-terminal myristoylation on the Ca2+-dependent conformational transition in recoverin.
  J Biol Chem, 278, 22972-22979.
PDB codes: 1omr 1omv
12562756 S.S.Lin, J.K.Manchester, and J.I.Gordon (2003).
Sip2, an N-myristoylated beta subunit of Snf1 kinase, regulates aging in Saccharomyces cerevisiae by affecting cellular histone kinase activity, recombination at rDNA loci, and silencing.
  J Biol Chem, 278, 13390-13397.  
12717707 T.Kawasaki, T.Nishio, H.Kurosawa, J.Roder, and A.Jeromin (2003).
Spatiotemporal distribution of neuronal calcium sensor-1 in the developing rat spinal cord.
  J Comp Neurol, 460, 465-475.  
14512421 T.Strahl, B.Grafelmann, J.Dannenberg, J.Thorner, and O.Pongs (2003).
Conservation of regulatory function in calcium-binding proteins: human frequenin (neuronal calcium sensor-1) associates productively with yeast phosphatidylinositol 4-kinase isoform, Pik1.
  J Biol Chem, 278, 49589-49599.  
12928444 X.Ren, S.H.Shand, and K.Takimoto (2003).
Effective association of Kv channel-interacting proteins with Kv4 channel is mediated with their unique core peptide.
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11836243 D.W.O'Callaghan, L.Ivings, J.L.Weiss, M.C.Ashby, A.V.Tepikin, and R.D.Burgoyne (2002).
Differential use of myristoyl groups on neuronal calcium sensor proteins as a determinant of spatio-temporal aspects of Ca2+ signal transduction.
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12070350 F.J.Quintero, M.Ohta, H.Shi, J.K.Zhu, and J.M.Pardo (2002).
Reconstitution in yeast of the Arabidopsis SOS signaling pathway for Na+ homeostasis.
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11799182 H.Hatanaka, O.Iourin, Z.Rao, E.Fry, A.Kingsman, and D.I.Stuart (2002).
Structure of equine infectious anemia virus matrix protein.
  J Virol, 76, 1876-1883.
PDB code: 1hek
12393897 I.I.Senin, T.Fischer, K.E.Komolov, D.V.Zinchenko, P.P.Philippov, and K.W.Koch (2002).
Ca2+-myristoyl switch in the neuronal calcium sensor recoverin requires different functions of Ca2+-binding sites.
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11980481 J.B.Ames, N.Hamasaki, and T.Molchanova (2002).
Structure and calcium-binding studies of a recoverin mutant (E85Q) in an allosteric intermediate state.
  Biochemistry, 41, 5776-5787.
PDB code: 1la3
12006572 K.Takimoto, E.K.Yang, and L.Conforti (2002).
Palmitoylation of KChIP splicing variants is required for efficient cell surface expression of Kv4.3 channels.
  J Biol Chem, 277, 26904-26911.  
12202488 L.Lin, E.M.Jeanclos, M.Treuil, K.H.Braunewell, E.D.Gundelfinger, and R.Anand (2002).
The calcium sensor protein visinin-like protein-1 modulates the surface expression and agonist sensitivity of the alpha 4beta 2 nicotinic acetylcholine receptor.
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12023256 P.Desmeules, M.Grandbois, V.A.Bondarenko, A.Yamazaki, and C.Salesse (2002).
Measurement of membrane binding between recoverin, a calcium-myristoyl switch protein, and lipid bilayers by AFM-based force spectroscopy.
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12354775 R.Rousset, K.A.Wharton, G.Zimmermann, and M.P.Scott (2002).
Zinc-dependent interaction between dishevelled and the Drosophila Wnt antagonist naked cuticle.
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11832217 S.Liemann, K.Chandran, T.S.Baker, M.L.Nibert, and S.C.Harrison (2002).
Structure of the reovirus membrane-penetration protein, Mu1, in a complex with is protector protein, Sigma3.
  Cell, 108, 283-295.
PDB code: 1jmu
11872749 T.Berggård, S.Miron, P.Onnerfjord, E.Thulin, K.S.Akerfeldt, J.J.Enghild, M.Akke, and S.Linse (2002).
Calbindin D28k exhibits properties characteristic of a Ca2+ sensor.
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12023286 W.T.Barry, C.Boudignon-Proudhon, D.D.Shock, A.McFadden, J.M.Weiss, J.Sondek, and L.V.Parise (2002).
Molecular basis of CIB binding to the integrin alpha IIb cytoplasmic domain.
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11319767 A.Orito, H.Kumanogoh, K.Yasaka, J.Sokawa, H.Hidaka, Y.Sokawa, and S.Maekawa (2001).
Calcium-dependent association of annexin VI, protein kinase C alpha, and neurocalcin alpha on the raft fraction derived from the synaptic plasma membrane of rat brain.
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11141074 A.Tholey, R.Pipkorn, D.Bossemeyer, V.Kinzel, and J.Reed (2001).
Influence of myristoylation, phosphorylation, and deamidation on the structural behavior of the N-terminus of the catalytic subunit of cAMP-dependent protein kinase.
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11732314 C.Plieth (2001).
Plant calcium signaling and monitoring: pros and cons and recent experimental approaches.
  Protoplasma, 218, 1.  
11266596 H.Aitio, T.Laakso, T.Pihlajamaa, M.Torkkeli, I.Kilpeläinen, T.Drakenberg, R.Serimaa, and A.Annila (2001).
Characterization of apo and partially saturated states of calerythrin, an EF-hand protein from S. erythraea: a molten globule when deprived of Ca(2+).
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11524415 I.Sokal, N.Li, C.S.Klug, S.Filipek, W.L.Hubbell, W.Baehr, and K.Palczewski (2001).
Calcium-sensitive regions of GCAP1 as observed by chemical modifications, fluorescence, and EPR spectroscopies.
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11566136 M.Han, V.V.Gurevich, S.A.Vishnivetskiy, P.B.Sigler, and C.Schubert (2001).
Crystal structure of beta-arrestin at 1.9 A: possible mechanism of receptor binding and membrane Translocation.
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PDB codes: 1g4m 1g4r
11527981 T.A.Farazi, G.Waksman, and J.I.Gordon (2001).
The biology and enzymology of protein N-myristoylation.
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10799589 A.Ono, D.Demirov, and E.O.Freed (2000).
Relationship between human immunodeficiency virus type 1 Gag multimerization and membrane binding.
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10733970 B.Z.Peterson, J.S.Lee, J.G.Mulle, Y.Wang, Leon, and D.T.Yue (2000).
Critical determinants of Ca(2+)-dependent inactivation within an EF-hand motif of L-type Ca(2+) channels.
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10625670 F.Haeseleer, I.Sokal, C.L.Verlinde, H.Erdjument-Bromage, P.Tempst, A.N.Pronin, J.L.Benovic, R.N.Fariss, and K.Palczewski (2000).
Five members of a novel Ca(2+)-binding protein (CABP) subfamily with similarity to calmodulin.
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11015193 J.B.Ames, K.B.Hendricks, T.Strahl, I.G.Huttner, N.Hamasaki, and J.Thorner (2000).
Structure and calcium-binding properties of Frq1, a novel calcium sensor in the yeast Saccharomyces cerevisiae.
  Biochemistry, 39, 12149-12161.
PDB code: 1fpw
10723031 K.Palczewski, A.S.Polans, W.Baehr, and J.B.Ames (2000).
Ca(2+)-binding proteins in the retina: structure, function, and the etiology of human visual diseases.
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The binding of myristoylated N-terminal nonapeptide from neuro-specific protein CAP-23/NAP-22 to calmodulin does not induce the globular structure observed for the calmodulin-nonmyristylated peptide complex.
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10652319 S.Tachibanaki, K.Nanda, K.Sasaki, K.Ozaki, and S.Kawamura (2000).
Amino acid residues of S-modulin responsible for interaction with rhodopsin kinase.
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10757969 S.Y.Lee, and R.E.Klevit (2000).
The whole is not the simple sum of its parts in calmodulin from S. cerevisiae.
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Transient-state kinetic analysis of Saccharomyces cerevisiae myristoylCoA:protein N-myristoyltransferase reveals that a step after chemical transformation is rate limiting.
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10652348 T.J.Melia, J.A.Malinski, F.He, and T.G.Wensel (2000).
Enhancement of phototransduction protein interactions by lipid surfaces.
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11087403 T.Ozawa, M.Fukuda, M.Nara, A.Nakamura, Y.Komine, K.Kohama, and Y.Umezawa (2000).
How can Ca2+ selectively activate recoverin in the presence of Mg2+? Surface plasmon resonance and FT-IR spectroscopic studies.
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10679893 V.Z.Slepak (2000).
Application of surface plasmon resonance for analysis of protein-protein interactions in the G protein-mediated signal transduction pathway.
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10734184 W.G.Krebs, and M.Gerstein (2000).
The morph server: a standardized system for analyzing and visualizing macromolecular motions in a database framework.
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10788460 X.Zhu, A.L.Boman, J.Kuai, W.Cieplak, and R.A.Kahn (2000).
Effectors increase the affinity of ADP-ribosylation factor for GTP to increase binding.
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10512975 A.Jeromin, A.J.Shayan, M.Msghina, J.Roder, and H.L.Atwood (1999).
Crustacean frequenins: molecular cloning and differential localization at neuromuscular junctions.
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Binding of human immunodeficiency virus type 1 Gag to membrane: role of the matrix amino terminus.
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Identification of the calmodulin-binding domain of neuron-specific protein kinase C substrate protein CAP-22/NAP-22. Direct involvement of protein myristoylation in calmodulin-target protein interaction.
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Neuronal Ca(2+) sensor 1. Characterization of the myristoylated protein, its cellular effects in permeabilized adrenal chromaffin cells, Ca(2+)-independent membrane association, and interaction with binding proteins, suggesting a role in rapid Ca(2+) signal transduction.
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Crystal structure of Escherichia coli lytic transglycosylase Slt35 reveals a lysozyme-like catalytic domain with an EF-hand.
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PDB codes: 1qus 1qut
10464292 E.V.Olshevskaya, A.N.Ermilov, and A.M.Dizhoor (1999).
Dimerization of guanylyl cyclase-activating protein and a mechanism of photoreceptor guanylyl cyclase activation.
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Mapping functional domains of the guanylate cyclase regulator protein, GCAP-2.
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Conformational changes in guanylyl cyclase-activating protein 1 (GCAP1) and its tryptophan mutants as a function of calcium concentration.
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Three-dimensional structure of guanylyl cyclase activating protein-2, a calcium-sensitive modulator of photoreceptor guanylyl cyclases.
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PDB code: 1jba
10396602 J.C.DeMar, D.R.Rundle, T.G.Wensel, and R.E.Anderson (1999).
Heterogeneous N-terminal acylation of retinal proteins.
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Opposing effects of human immunodeficiency virus type 1 matrix mutations support a myristyl switch model of gag membrane targeting.
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Diversity of conformational states and changes within the EF-hand protein superfamily.
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10026293 L.Chen, G.J.Pielak, and N.L.Thompson (1999).
The cytoplasmic region of mouse Fc gamma RIIb1, but not Fc gamma RIIb2, binds phospholipid membranes.
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10202162 M.Rodríguez-Concepción, S.Yalovsky, M.Zik, H.Fromm, and W.Gruissem (1999).
The prenylation status of a novel plant calmodulin directs plasma membrane or nuclear localization of the protein.
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10470373 R.A.Clegg, P.C.Gordge, and W.R.Miller (1999).
Expression of enzymes of covalent protein modification during regulated and dysregulated proliferation of mammary epithelial cells: PKA, PKC and NMT.
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A myristoylated calcium-binding protein that preferentially interacts with the Alzheimer's disease presenilin 2 protein.
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Role of carboxyl-terminal charges on S-modulin membrane affinity and inhibition of rhodopsin phosphorylation.
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10574794 S.Rothemund, Y.C.Liou, P.L.Davies, E.Krause, and F.D.Sönnichsen (1999).
A new class of hexahelical insect proteins revealed as putative carriers of small hydrophobic ligands.
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PDB codes: 1c3y 1c3z
9860856 A.N.Baldwin, and J.B.Ames (1998).
Core mutations that promote the calcium-induced allosteric transition of bovine recoverin.
  Biochemistry, 37, 17408-17419.  
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Structural basis for activation of ARF GTPase: mechanisms of guanine nucleotide exchange and GTP-myristoyl switching.
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2H NMR studies of a myristoylated peptide in neutral and acidic phospholipid bicelles.
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9668070 M.B.Meyers, T.S.Puri, A.J.Chien, T.Gao, P.H.Hsu, M.M.Hosey, and G.I.Fishman (1998).
Sorcin associates with the pore-forming subunit of voltage-dependent L-type Ca2+ channels.
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Acylation of Escherichia coli hemolysin: a unique protein lipidation mechanism underlying toxin function.
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Ca2+-dependent conformational changes in bovine GCAP-2.
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9685370 S.Matsuda, O.Hisatomi, T.Ishino, Y.Kobayashi, and F.Tokunaga (1998).
The role of calcium-binding sites in S-modulin function.
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A novel calcium-sensitive switch revealed by the structure of human S100B in the calcium-bound form.
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PDB code: 1uwo
9819203 X.Shao, I.Fernandez, T.C.Südhof, and J.Rizo (1998).
Solution structures of the Ca2+-free and Ca2+-bound C2A domain of synaptotagmin I: does Ca2+ induce a conformational change?
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PDB code: 1byn
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Nitric oxide synthases: which, where, how, and why?
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