PDBsum entry: 1jja

Hydrolase

PDB id: 1jja

Contents

Protein chains

309 a.a. *

326 a.a. *

Waters ×444

* Residue conservation analysis

PDB id:

1jja

Name:

Hydrolase

Title:

Crystal structure of orthorhombic form of d90e mutant of escherichia coli l-asparaginase ii

Structure:

L-asparaginase ii. Chain: a, b, c, d, e, f. Synonym: l-asnase ii. Engineered: yes. Mutation: yes

Source:

Escherichia coli. Organism_taxid: 562. Expressed in: escherichia coli. Expression_system_taxid: 562

Biol. unit:

Tetramer (from PDB file)

Resolution:

2.30Å R-factor: 0.187 R-free: 0.204

Authors:

D.Borek,M.Kozak,M.Jaskolski

Key ref:

D.Borek et al. Structure analysis of d90e mutant of e. Coli asparaginase ii in three crystal forms. To be published,

Date:

04-Jul-01 Release date: 09-Sep-03

Protein chains

P00805  (ASPG2_ECOLI) -  L-asparaginase 2

Seq: 348 a.a.

Struc: 309 a.a.*

Protein chains

P00805  (ASPG2_ECOLI) -  L-asparaginase 2

Seq: 348 a.a.

Struc: 326 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chains A, B, C, D, E, F: E.C.3.5.1.1 - Asparaginase.
• Reaction: L-asparagine + H₂O = L-aspartate + NH₃

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

 Gene Ontology (GO) functional annotation 

• Cellular component: periplasmic space (2 terms)
• Biological process: cellular amino acid metabolic process (2 terms)
• Biochemical function: hydrolase activity (2 terms)