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Lipid transport PDB id
1jfn
Jmol
Contents
Protein chain
119 a.a. *
* Residue conservation analysis
PDB id:
1jfn
Name: Lipid transport
Title: Solution structure of human apolipoprotein(a) kringle iv type 6
Structure: Apolipoprotein a, kiv-t6. Chain: a. Fragment: apo(a) kiv-t6. Synonym: apo(a) kiv-t6. Lipoprotein, lp(a). Apolipoprotein lp(a). Apo(a). Lp(a). Engineered: yes
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: apoa. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
NMR struc: 15 models
Authors: B.Maderegger,W.Bermel,A.Hrzenjak,G.M.Kostner,H.Sterk
Key ref:
B.Maderegger et al. (2002). Solution structure of human apolipoprotein(a) kringle IV type 6. Biochemistry, 41, 660-668. PubMed id: 11781107 DOI: 10.1021/bi011430k
Date:
21-Jun-01     Release date:   28-Jun-02    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P08519  (APOA_HUMAN) -  Apolipoprotein(a)
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Struc: 		4548 a.a.
119 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 13 residue positions (black crosses)

 

 
DOI no: 10.1021/bi011430k Biochemistry 41:660-668 (2002)
PubMed id: 11781107  
 
 
Solution structure of human apolipoprotein(a) kringle IV type 6.
B.Maderegger, W.Bermel, A.Hrzenjak, G.M.Kostner, H.Sterk.
 
  ABSTRACT  
 
The structure of apo(a) KIVT6 was investigated by two- and three-dimensional homo- and heteronuclear NMR spectroscopy. The solution structure of apo(a) KIVT6 contains only a small amount of regular secondary structure elements, comprising a short piece of antiparallel beta-sheet formed by residues Trp62-Tyr64 and Trp72-Tyr74, a short piece of parallel beta-sheet formed by the residues Cys1-Tyr2 and Thr78-Gln79, and a small 3(10)-helix within residues Thr38-Tyr40. The backbone as well as the side chains are arranged in a way similar to those of apo(a) KIVT7, apo(a) KIVT10, and plasminogen K4. We determined additionally the K(d) value of 0.31 +/- 0.04 mM for the binding of epsilon-aminocaproic acid (EACA) to apo(a) KIVT6 and mapped the binding region on apo(a) KIVT6 by means of chemical shift perturbation. This lysine binding activity, which was reported to occur within apo(a) KIVT5-8, is functionally different from the lysine binding activity found for apo(a) KIVT10.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
20050964 X.Yang, W.Cai, Z.Xu, J.Chen, C.Li, S.Liu, Z.Yang, Q.Pan, M.Li, J.Ma, and G.Gao (2010).
High efficacy and minimal peptide required for the anti-angiogenic and anti-hepatocarcinoma activities of plasminogen K5.
  J Cell Mol Med, 14, 2519-2530.  
17391015 S.Vucetic, H.Xie, L.M.Iakoucheva, C.J.Oldfield, A.K.Dunker, Z.Obradovic, and V.N.Uversky (2007).
Functional anthology of intrinsic disorder. 2. Cellular components, domains, technical terms, developmental processes, and coding sequence diversities correlated with long disordered regions.
  J Proteome Res, 6, 1899-1916.  
14717962 J.H.Geiger, and S.E.Cnudde (2004).
What the structure of angiostatin may tell us about its mechanism of action.
  J Thromb Haemost, 2, 23-34.  
14581473 L.Becker, P.M.Cook, T.G.Wright, and M.L.Koschinsky (2004).
Quantitative evaluation of the contribution of weak lysine-binding sites present within apolipoprotein(a) kringle IV types 6-8 to lipoprotein(a) assembly.
  J Biol Chem, 279, 2679-2688.  
15017359 M.L.Koschinsky, and S.M.Marcovina (2004).
Structure-function relationships in apolipoprotein(a): insights into lipoprotein(a) assembly and pathogenicity.
  Curr Opin Lipidol, 15, 167-174.  
  18516206 S.P.McCormick (2004).
Lipoprotein(a): Biology and Clinical Importance.
  Clin Biochem Rev, 25, 69-80.  
12151854 K.M.Kostner, and G.M.Kostner (2002).
Lipoprotein(a): still an enigma?
  Curr Opin Lipidol, 13, 391-396.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time.