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PDBsum entry 1j4b

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protein links
Ligase PDB id
1j4b
Jmol
Contents
Protein chain
432 a.a. *
Waters ×96
* Residue conservation analysis
PDB id:
1j4b
Name: Ligase
Title: Recombinant mouse-muscle adenylosuccinate synthetase
Structure: Adenylosuccinate synthetase. Chain: a. Synonym: adss1. Engineered: yes
Source: Mus musculus. House mouse. Organism_taxid: 10090. Tissue: muscle. Cellular_location: cytoplasm. Gene: adss1. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Biol. unit: Dimer (from PDB file)
Resolution:
2.50Å     R-factor:   0.207     R-free:   0.272
Authors: C.V.Iancu,T.Borza,J.Y.Choe,H.J.Fromm,R.B.Honzatko
Key ref:
C.V.Iancu et al. (2001). Recombinant mouse muscle adenylosuccinate synthetase: overexpression, kinetics, and crystal structure. J Biol Chem, 276, 42146-42152. PubMed id: 11560929 DOI: 10.1074/jbc.M106294200
Date:
07-Sep-01     Release date:   21-Nov-01    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
P28650  (PURA1_MOUSE) -  Adenylosuccinate synthetase isozyme 1
Seq:
Struc:
457 a.a.
432 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: E.C.6.3.4.4  - Adenylosuccinate synthase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]

      Pathway:
AMP and GMP Biosynthesis
      Reaction: GTP + IMP + L-aspartate = GDP + phosphate + N6-(1,2-dicarboxyethyl)- AMP
GTP
+ IMP
+ L-aspartate
= GDP
+ phosphate
+ N(6)-(1,2-dicarboxyethyl)- AMP
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     membrane   2 terms 
  Biological process     cellular response to drug   13 terms 
  Biochemical function     nucleotide binding     10 terms  

 

 
    reference    
 
 
DOI no: 10.1074/jbc.M106294200 J Biol Chem 276:42146-42152 (2001)
PubMed id: 11560929  
 
 
Recombinant mouse muscle adenylosuccinate synthetase: overexpression, kinetics, and crystal structure.
C.V.Iancu, T.Borza, J.Y.Choe, H.J.Fromm, R.B.Honzatko.
 
  ABSTRACT  
 
Vertebrates possess two isozymes of adenylosuccinate synthetase. The acidic isozyme is similar to the synthetase from bacteria and plants, being involved in the de novo biosynthesis of AMP, whereas the basic isozyme participates in the purine nucleotide cycle. Reported here is the first instance of overexpression and crystal structure determination of a basic isozyme of adenylosuccinate synthetase. The recombinant mouse muscle enzyme purified to homogeneity in milligram quantities exhibits a specific activity comparable with that of the rat muscle enzyme isolated from tissue and K(m) parameters for GTP, IMP, and l-aspartate (12, 45, and 140 microm, respectively) similar to those of the enzyme from Escherichia coli. The mouse muscle and E. coli enzymes have similar polypeptide folds, differing primarily in the conformation of loops, involved in substrate recognition and stabilization of the transition state. Residues 65-68 of the muscle isozyme adopt a conformation not observed in any previous synthetase structure. In its new conformation, segment 65-68 forms intramolecular hydrogen bonds with residues essential for the recognition of IMP and, in fact, sterically excludes IMP from the active site. Observed differences in ligand recognition among adenylosuccinate synthetases may be due in part to conformational variations in the IMP pocket of the ligand-free enzymes.
 
  Selected figure(s)  
 
Figure 3.
Fig. 3. Subunit interface of mouse muscle adenylosuccinate synthetase. Top panel, overview of the subunits in the dimer as viewed down its molecular 2-fold axis. Bottom panel, a more detailed presentation of side chain interactions. The viewing orientation is the same for the top and bottom panels. This figure was drawn by MOLSCRIPT (47).
Figure 4.
Fig. 4. Stereoview of segment 65-68 of the mouse muscle synthetase. Top panel, residues 65-68 and associated omit electron density contoured at 1 with a cut-off radius of 1 Å. Middle and bottom panels, conformational differences between residues 65-68 of the mouse muscle synthetase (bold lines) and corresponding residues of the ligand-free (middle panel) and ligated (bottom panel) E. coli enzyme. The dashed lines represent donor-acceptor interactions.
 
  The above figures are reprinted by permission from the ASBMB: J Biol Chem (2001, 276, 42146-42152) copyright 2001.  
  Figures were selected by an automated process.  

Literature references that cite this PDB file's key reference

  PubMed id Reference
17634986 E.R.Jefferson, T.P.Walsh, and G.J.Barton (2008).
A comparison of SCOP and CATH with respect to domain-domain interactions.
  Proteins, 70, 54-62.  
15786719 H.Sun, N.Li, X.Wang, T.Chen, L.Shi, L.Zhang, J.Wang, T.Wan, and X.Cao (2005).
Molecular cloning and characterization of a novel muscle adenylosuccinate synthetase, AdSSL1, from human bone marrow stromal cells.
  Mol Cell Biochem, 269, 85-94.  
12169208 H.Y.Wen, Y.Xia, M.E.Young, H.Taegtmeyer, and R.E.Kellems (2002).
The adenylosuccinate synthetase-1 gene is activated in the hypertrophied heart.
  J Cell Mol Med, 6, 235-243.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time.