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Cytokine PDB id
1inr
Jmol
Contents
Protein chain
132 a.a. *
Waters ×42
* Residue conservation analysis
PDB id:
1inr
Name: Cytokine
Title: Cytokine synthesis
Structure: Interleukin-10. Chain: a. Synonym: cytokine synthesis inhibitory factor, csif. Engineered: yes
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 562
Biol. unit: Dimer (from PDB file)
Resolution:
2.00Å     R-factor:   0.221     R-free:   0.337
Authors: M.R.Walter
Key ref:
M.R.Walter and T.L.Nagabhushan (1995). Crystal structure of interleukin 10 reveals an interferon gamma-like fold. Biochemistry, 34, 12118-12125. PubMed id: 7547951 DOI: 10.1021/bi00038a004
Date:
31-Jul-95     Release date:   14-Oct-96    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P22301  (IL10_HUMAN) -  Interleukin-10
Seq:
Struc: 		178 a.a.
132 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     extracellular region   3 terms 
  Biological process     negative regulation of growth of symbiont in host   51 terms 
  Biochemical function     cytokine activity     3 terms  

 

 
DOI no: 10.1021/bi00038a004 Biochemistry 34:12118-12125 (1995)
PubMed id: 7547951  
 
 
Crystal structure of interleukin 10 reveals an interferon gamma-like fold.
M.R.Walter, T.L.Nagabhushan.
 
  ABSTRACT  
 
The crystal structure of recombinant human interleukin 10 (rhIL-10) has been determined by X-ray crystallography at 2.0 A resolution. Interleukin 10 is a dimer composed of identical polypeptide chains related by a 2-fold axis. The molecule is predominantly alpha-helical. The main-chain fold resembles that of interferon gamma (IFN-gamma) in which the structural integrity of each domain is dependent on the intertwining of helices from each peptide chain. Comparison of rhIL-10 and IFN-gamma reveals differences in helix lengths and orientations of the 2-fold related domains. Interleukin 10 and IFN-gamma contain several conserved residues in their internal cores which suggest a possible "fingerprint" for detection of other members of this fold.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
21166540 W.Ouyang, S.Rutz, N.K.Crellin, P.A.Valdez, and S.G.Hymowitz (2011).
Regulation and functions of the IL-10 family of cytokines in inflammation and disease.
  Annu Rev Immunol, 29, 71.  
20846897 A.Zdanov (2010).
Structural analysis of cytokines comprising the IL-10 family.
  Cytokine Growth Factor Rev, 21, 325-330.  
20454917 D.B.Trivella, J.R.Ferreira-Júnior, L.Dumoutier, J.C.Renauld, and I.Polikarpov (2010).
Structure and function of interleukin-22 and other members of the interleukin-10 family.
  Cell Mol Life Sci, 67, 2909-2935.  
20712454 H.H.Gad, O.J.Hamming, and R.Hartmann (2010).
The structure of human interferon lambda and what it has taught us.
  J Interferon Cytokine Res, 30, 565-571.  
20947410 R.P.Donnelly, F.Sheikh, H.Dickensheets, R.Savan, H.A.Young, and M.R.Walter (2010).
Interleukin-26: an IL-10-related cytokine produced by Th17 cells.
  Cytokine Growth Factor Rev, 21, 393-401.  
21112807 R.Sabat (2010).
IL-10 family of cytokines.
  Cytokine Growth Factor Rev, 21, 315-324.  
21115385 R.Sabat, G.Grütz, K.Warszawska, S.Kirsch, E.Witte, K.Wolk, and J.Geginat (2010).
Biology of interleukin-10.
  Cytokine Growth Factor Rev, 21, 331-344.  
20864382 U.M.Wegenka (2010).
IL-20: biological functions mediated through two types of receptor complexes.
  Cytokine Growth Factor Rev, 21, 353-363.  
  18391423 B.C.Jones, N.J.Logsdon, and M.R.Walter (2008).
Crystallization and preliminary X-ray diffraction analysis of human IL-22 bound to the extracellular IL-22R1 chain.
  Acta Crystallogr Sect F Struct Biol Cryst Commun, 64, 266-269.  
18599299 B.C.Jones, N.J.Logsdon, and M.R.Walter (2008).
Structure of IL-22 bound to its high-affinity IL-22R1 chain.
  Structure, 16, 1333-1344.
PDB code: 3dgc
18216121 C.Jenkins, W.Garcia, M.J.Godwin, J.V.Spencer, J.L.Stern, A.Abendroth, and B.Slobedman (2008).
Immunomodulatory properties of a viral homolog of human interleukin-10 expressed by human cytomegalovirus during the latent phase of infection.
  J Virol, 82, 3736-3750.  
18024507 M.de Oliveira Neto, J.R.Ferreira, D.Colau, H.Fischer, A.S.Nascimento, A.F.Craievich, L.Dumoutier, J.C.Renauld, and I.Polikarpov (2008).
Interleukin-22 forms dimers that are recognized by two interleukin-22R1 receptor chains.
  Biophys J, 94, 1754-1765.  
19040572 W.Li, A.Lewis-Antes, J.Huang, M.Balan, and S.V.Kotenko (2008).
Regulation of apoptosis by type III interferons.
  Cell Prolif, 41, 960-979.  
17224278 S.I.Yoon, and M.R.Walter (2007).
Identification and characterization of a +1 frameshift observed during the expression of Epstein-Barr virus IL-10 in Escherichia coli.
  Protein Expr Purif, 53, 132-137.  
17030002 K.Wolk, and R.Sabat (2006).
Interleukin-22: a novel T- and NK-cell derived cytokine that regulates the biology of tissue cells.
  Cytokine Growth Factor Rev, 17, 367-380.  
15837194 S.I.Yoon, B.C.Jones, N.J.Logsdon, and M.R.Walter (2005).
Same structure, different function crystal structure of the Epstein-Barr virus IL-10 bound to the soluble IL-10R1 chain.
  Structure, 13, 551-564.
PDB codes: 1y6k 1y6m 1y6n
15985167 S.Pletnev, E.Magracheva, A.Wlodawer, and A.Zdanov (2005).
A model of the ternary complex of interleukin-10 with its soluble receptors.
  BMC Struct Biol, 5, 10.  
15983417 T.Xu, N.J.Logsdon, and M.R.Walter (2005).
Structure of insect-cell-derived IL-22.
  Acta Crystallogr D Biol Crystallogr, 61, 942-950.
PDB code: 1ykb
15032600 S.Pestka, C.D.Krause, D.Sarkar, M.R.Walter, Y.Shi, and P.B.Fisher (2004).
Interleukin-10 and related cytokines and receptors.
  Annu Rev Immunol, 22, 929-979.  
15213397 T.Xu, N.J.Logsdon, and M.R.Walter (2004).
Crystallization and X-ray diffraction analysis of insect-cell-derived IL-22.
  Acta Crystallogr D Biol Crystallogr, 60, 1295-1298.  
12403790 C.Chang, E.Magracheva, S.Kozlov, S.Fong, G.Tobin, S.Kotenko, A.Wlodawer, and A.Zdanov (2003).
Crystal structure of interleukin-19 defines a new subfamily of helical cytokines.
  J Biol Chem, 278, 3308-3313.
PDB code: 1n1f
12869211 G.Lutfalla, H.Roest Crollius, N.Stange-Thomann, O.Jaillon, K.Mogensen, and D.Monneron (2003).
Comparative genomic analysis reveals independent expansion of a lineage-specific gene family in vertebrates: the class II cytokine receptors and their ligands in mammals and fish.
  BMC Genomics, 4, 29.  
12974481 J.C.Renauld (2003).
Class II cytokine receptors and their ligands: key antiviral and inflammatory modulators.
  Nat Rev Immunol, 3, 667-676.  
12093920 B.C.Jones, N.J.Logsdon, K.Josephson, J.Cook, P.A.Barry, and M.R.Walter (2002).
Crystal structure of human cytomegalovirus IL-10 bound to soluble human IL-10R1.
  Proc Natl Acad Sci U S A, 99, 9404-9409.
PDB code: 1lqs
  11929132 H.Fickenscher, S.Hör, H.Küpers, A.Knappe, S.Wittmann, and H.Sticht (2002).
The interleukin-10 family of cytokines.
  Trends Immunol, 23, 89-96.  
  12121653 K.Josephson, B.C.Jones, L.J.Walter, R.DiGiacomo, S.R.Indelicato, and M.R.Walter (2002).
Noncompetitive antibody neutralization of IL-10 revealed by protein engineering and x-ray crystallography.
  Structure, 10, 981-987.
PDB code: 1lk3
11970958 K.Vandenbroeck, I.Alloza, D.Brehmer, A.Billiau, P.Proost, N.McFerran, S.Rüdiger, and B.Walker (2002).
The conserved helix C region in the superfamily of interferon-gamma /interleukin-10-related cytokines corresponds to a high-affinity binding site for the HSP70 chaperone DnaK.
  J Biol Chem, 277, 25668-25676.  
12513909 N.J.Logsdon, B.C.Jones, K.Josephson, J.Cook, and M.R.Walter (2002).
Comparison of interleukin-22 and interleukin-10 soluble receptor complexes.
  J Interferon Cytokine Res, 22, 1099-1112.  
  12486876 S.V.Kotenko (2002).
The family of IL-10-related cytokines and their receptors: related, but to what extent?
  Cytokine Growth Factor Rev, 13, 223-240.  
11717514 K.Josephson, D.T.McPherson, and M.R.Walter (2001).
Purification, crystallization and preliminary X-ray diffraction of a complex between IL-10 and soluble IL-10R1.
  Acta Crystallogr D Biol Crystallogr, 57, 1908-1911.  
11244051 K.W.Moore, R.de Waal Malefyt, R.L.Coffman, and A.O'Garra (2001).
Interleukin-10 and the interleukin-10 receptor.
  Annu Rev Immunol, 19, 683-765.  
  11250200 M.Randal, and A.A.Kossiakoff (2001).
The structure and activity of a monomeric interferon-gamma:alpha-chain receptor signaling complex.
  Structure, 9, 155-163.
PDB code: 1fyh
11060663 K.Asadullah, W.D.Döcke, R.V.Sabat, H.D.Volk, and W.Sterry (2000).
The treatment of psoriasis with IL-10: rationale and review of the first clinical trials.
  Expert Opin Investig Drugs, 9, 95.  
10788470 K.Josephson, R.DiGiacomo, S.R.Indelicato, A.H.Iyo, T.L.Nagabhushan, M.H.Parker, M.R.Walter, and A.H.Ayo (2000).
Design and analysis of an engineered human interleukin-10 monomer.
  J Biol Chem, 275, 13552-13557.  
10666622 M.Randal, and A.A.Kossiakoff (2000).
The 2.0 A structure of bovine interferon-gamma; assessment of the structural differences between species.
  Acta Crystallogr D Biol Crystallogr, 56, 14-24.
PDB codes: 1d9c 1d9g
10677520 S.V.Kotenko, S.Saccani, L.S.Izotova, O.V.Mirochnitchenko, and S.Pestka (2000).
Human cytomegalovirus harbors its own unique IL-10 homolog (cmvIL-10).
  Proc Natl Acad Sci U S A, 97, 1695-1700.  
10637267 Y.Ding, L.Qin, S.V.Kotenko, S.Pestka, and J.S.Bromberg (2000).
A single amino acid determines the immunostimulatory activity of interleukin 10.
  J Exp Med, 191, 213-224.  
10231374 D.M.Hoover, C.Schalk-Hihi, C.C.Chou, S.Menon, A.Wlodawer, and A.Zdanov (1999).
Purification of receptor complexes of interleukin-10 stoichiometry and the importance of deglycosylation in their crystallization.
  Eur J Biochem, 262, 134-141.  
10547147 K.E.Mogensen, M.Lewerenz, J.Reboul, G.Lutfalla, and G.Uzé (1999).
The type I interferon receptor: structure, function, and evolution of a family business.
  J Interferon Cytokine Res, 19, 1069-1098.  
  9646174 P.Stordeur, and M.Goldman (1998).
Interleukin-10 as a regulatory cytokine induced by cellular stress: molecular aspects.
  Int Rev Immunol, 16, 501-522.  
9463407 S.D.Spencer, F.Di Marco, J.Hooley, S.Pitts-Meek, M.Bauer, A.M.Ryan, B.Sordat, V.C.Gibbs, and M.Aguet (1998).
The orphan receptor CRF2-4 is an essential subunit of the interleukin 10 receptor.
  J Exp Med, 187, 571-578.  
  9568901 U.Reineke, R.Sabat, H.D.Volk, and J.Schneider-Mergener (1998).
Mapping of the interleukin-10/interleukin-10 receptor combining site.
  Protein Sci, 7, 951-960.  
  9144766 R.J.Simpson, A.Hammacher, D.K.Smith, J.M.Matthews, and L.D.Ward (1997).
Interleukin-6: structure-function relationships.
  Protein Sci, 6, 929-955.  
  8897595 A.Zdanov, C.Schalk-Hihi, and A.Wlodawer (1996).
Crystal structure of human interleukin-10 at 1.6 A resolution and a model of a complex with its soluble receptor.
  Protein Sci, 5, 1955-1962.
PDB code: 2ilk
  8994971 R.Radhakrishnan, L.J.Walter, A.Hruza, P.Reichert, P.P.Trotta, T.L.Nagabhushan, and M.R.Walter (1996).
Zinc mediated dimer of human interferon-alpha 2b revealed by X-ray crystallography.
  Structure, 4, 1453-1463.
PDB code: 1rh2
9237205 U.Reineke, R.Sabat, A.Kramer, R.D.Stigler, M.Seifert, T.Michel, H.D.Volk, and J.Schneider-Mergener (1996).
Mapping protein-protein contact sites using cellulose-bound peptide scans.
  Mol Divers, 1, 141-148.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.